FastQCFastQC Report
Thu 26 May 2016
SRR936476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335975
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64890.4857126817492842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51420.3848874417560209No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33890.253672411534647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31490.2357080035180299No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20320.15209865454069127No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.10995714740171036No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14450.10816070660004866No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13730.10277138419506353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15600.030.774662
GTACCTG15750.030.4815711
GTATCAA128700.023.9327721
GAGTACT61300.023.57139212-13
GTACATG83300.023.509791
TACATGG85300.022.5684912
ACATGGG83850.022.3368643
GTACTTT65950.021.6907714-15
ATCGCCG550.002876402321.59343124-25
GTCTACG1353.7785497E-421.125841
GTGTAGC6350.020.9594961
GGTATCA96500.020.9342861
AGTACTT63700.020.70597512-13
GTACCCG1856.5139775E-620.5548731
CATGGGA49400.020.304144
TCAACGC151000.020.1794874
GTATAGG4250.020.1316851
TATCAAC152200.020.1125772
ATCAACG151200.020.0899183
CAACGCA152300.020.0392045
Produced by FastQC (version 0.11.5)