##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936476_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1335975 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.375769756170587 31.0 31.0 34.0 30.0 34.0 2 31.407650592264076 31.0 31.0 34.0 28.0 34.0 3 31.351661520612286 31.0 31.0 34.0 28.0 34.0 4 35.03227605306986 37.0 35.0 37.0 32.0 37.0 5 35.08397911637568 37.0 35.0 37.0 32.0 37.0 6 35.150646531559346 37.0 35.0 37.0 32.0 37.0 7 35.01699507850072 37.0 35.0 37.0 32.0 37.0 8 35.004744100750386 37.0 35.0 37.0 32.0 37.0 9 36.48198506708584 39.0 37.0 39.0 32.0 39.0 10-11 36.36316285858642 39.0 37.0 39.0 32.0 39.0 12-13 36.274001010497955 39.0 35.0 39.0 32.0 39.0 14-15 37.23676453526451 39.5 37.0 41.0 31.5 41.0 16-17 37.16162914725201 39.0 36.0 41.0 31.0 41.0 18-19 37.06992870375568 39.0 36.0 41.0 31.0 41.0 20-21 37.06663859727914 39.0 36.0 41.0 31.0 41.0 22-23 37.063052826587324 39.0 36.0 41.0 31.0 41.0 24-25 37.16119575590861 39.0 36.0 41.0 31.0 41.0 26-27 37.080561013492016 39.0 36.0 41.0 31.0 41.0 28-29 36.960525084675986 39.0 36.0 41.0 30.5 41.0 30-31 36.73507326110144 39.0 36.0 41.0 30.0 41.0 32-33 36.537925110874085 39.0 35.0 41.0 30.0 41.0 34-35 36.329767398342035 39.0 35.0 40.5 30.0 41.0 36-37 36.09823424839537 38.5 35.0 40.0 29.5 41.0 38-39 35.891954190759556 38.0 35.0 40.0 29.0 41.0 40-41 35.641399352532794 38.0 35.0 40.0 27.5 41.0 42-43 35.34018825202567 38.0 34.5 40.0 26.5 41.0 44-45 35.131917139168024 38.0 34.0 40.0 26.0 41.0 46-47 34.895017122326394 38.0 34.0 40.0 24.5 41.0 48-49 34.59926420778832 38.0 34.0 40.0 24.0 41.0 50-51 33.585877729747935 37.5 32.5 39.5 22.5 40.5 52-53 33.67535769756171 37.5 33.0 39.5 22.5 40.5 54-55 34.11065364247085 38.0 34.0 40.0 23.0 41.0 56-57 33.95341866427141 38.0 33.0 40.0 22.0 41.0 58-59 33.72753906323098 37.5 33.0 40.0 21.5 41.0 60-61 33.42781900858923 37.0 33.0 40.0 20.0 41.0 62-63 33.089438799378726 36.5 32.5 40.0 18.5 41.0 64-65 32.730117704298365 36.0 32.0 39.0 18.0 41.0 66-67 32.360690132674634 35.5 32.0 39.0 16.0 41.0 68-69 31.941070379310986 35.0 31.0 39.0 13.0 40.0 70-71 31.541312524560716 35.0 31.0 38.0 9.5 40.0 72-73 31.131171616235335 35.0 31.0 37.0 7.5 39.5 74-75 30.72788300679279 35.0 30.0 37.0 7.0 39.0 76-77 30.281790078407155 34.5 30.0 36.0 3.5 39.0 78-79 29.82707236288104 34.0 29.0 36.0 2.0 38.5 80-81 29.39228204120586 34.0 29.0 35.0 2.0 37.0 82-83 29.000381369411855 34.0 29.0 35.0 2.0 37.0 84-85 28.636617077415373 34.0 29.0 35.0 2.0 36.0 86-87 28.304460787065626 34.0 28.0 35.0 2.0 36.0 88-89 27.964537135799695 34.0 27.0 35.0 2.0 36.0 90-91 27.617562828645745 34.0 27.0 35.0 2.0 35.0 92-93 27.20645446209697 33.0 26.0 35.0 2.0 35.0 94-95 26.7265933120006 33.0 25.0 35.0 2.0 35.0 96-97 26.14120174404461 33.0 24.5 35.0 2.0 35.0 98-99 25.424954434027583 33.0 21.0 35.0 2.0 35.0 100-101 22.920240648215724 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7622038797842912 1101 2 0.5845926367578862 1101 3 0.7713511411852174 1101 4 0.8078265778577105 1101 5 0.6895538631642069 1101 6 0.709859505234796 1101 7 0.7896956995135866 1101 8 0.7167611141514598 1101 9 0.857649438039843 1101 10-11 0.9978153860773205 1101 12-13 1.0142379375922488 1101 14-15 1.2094666485289736 1101 16-17 1.2219460378383573 1101 18-19 1.4956559608410132 1101 20-21 1.2164975748633182 1101 22-23 1.1912409008780074 1101 24-25 1.0368933344656384 1101 26-27 1.1720969613087178 1101 28-29 1.1720442292419122 1101 30-31 0.9511310046311934 1101 32-33 0.9334384386967898 1101 34-35 0.9345615831528633 1101 36-37 1.1168048427599615 1101 38-39 0.9819285529081156 1101 40-41 1.038367235969048 1101 42-43 1.1862771744193736 1101 44-45 1.1618925725384344 1101 46-47 1.0403361052274178 1101 48-49 1.2150719637316882 1101 50-51 0.9446454922203245 1101 52-53 1.0697183114589777 1101 54-55 1.2562772588059374 1101 56-57 1.330168239115487 1101 58-59 1.452079261316051 1101 60-61 1.1406551109133503 1101 62-63 1.243160104661392 1101 64-65 1.2041008198929077 1101 66-67 1.4270958813278298 1101 68-69 1.3079509266852085 1101 70-71 1.2521104506674412 1101 72-73 1.4299944044559112 1101 74-75 1.2942137547295829 1101 76-77 1.322566587792707 1101 78-79 1.210604113033746 1101 80-81 1.3897359821858792 1101 82-83 1.4411379992856972 1101 84-85 1.4094987637399328 1101 86-87 1.2718234688969936 1101 88-89 1.5662058625659583 1101 90-91 1.3294722082027377 1101 92-93 1.4881927945303026 1101 94-95 1.4643670405268203 1101 96-97 1.4667396220710636 1101 98-99 1.4863959908896085 1101 100-101 1.9147714987743036 1102 1 0.4413913361929005 1102 2 0.5752104274118892 1102 3 0.710081759780639 1102 4 0.6225162071296708 1102 5 0.6208605319769092 1102 6 0.5602203078529655 1102 7 0.6240685199438687 1102 8 0.5546883098164699 1102 9 0.6714068821087267 1102 10-11 0.7647456130208212 1102 12-13 0.574293466322338 1102 14-15 0.8082179227389688 1102 16-17 0.4365230001217384 1102 18-19 0.5662941388548575 1102 20-21 0.7481214548997954 1102 22-23 0.4602400424628499 1102 24-25 0.6921792940128242 1102 26-27 0.5169987800758236 1102 28-29 0.44864839255539835 1102 30-31 0.4045284508461151 1102 32-33 0.49196491543910525 1102 34-35 0.40003049242911004 1102 36-37 0.35405938967771533 1102 38-39 0.2817032189309714 1102 40-41 0.5441481255017493 1102 42-43 0.57221526122661 1102 44-45 0.4344326766212703 1102 46-47 0.4552691756711127 1102 48-49 0.4284820179191513 1102 50-51 0.4082164992485886 1102 52-53 0.3148528412084346 1102 54-55 0.5348396816827048 1102 56-57 0.525461039158408 1102 58-59 0.36128509317398283 1102 60-61 0.5402708360058952 1102 62-63 0.5187985509299651 1102 64-65 0.5793221300490288 1102 66-67 0.502166968832519 1102 68-69 0.4377929246464731 1102 70-71 0.223460845001906 1102 72-73 0.5040964217315143 1102 74-75 0.3718097899246047 1102 76-77 0.5274562009693753 1102 78-79 0.4200842353579084 1102 80-81 0.6810069731188726 1102 82-83 0.6648624480222196 1102 84-85 0.5705153150570652 1102 86-87 0.5099364309657766 1102 88-89 0.5393300913335999 1102 90-91 0.6365702284827925 1102 92-93 0.4089738181903684 1102 94-95 0.5257671961145682 1102 96-97 0.7339951078803892 1102 98-99 0.819233052963753 1102 100-101 1.2886582416253134 1103 1 0.43770577990328974 1103 2 0.5501761716189932 1103 3 0.6506296077827933 1103 4 0.571731994156508 1103 5 0.5569581854550947 1103 6 0.5416701842874261 1103 7 0.5076079997451899 1103 8 0.3894928071127026 1103 9 0.5137598880545582 1103 10-11 0.34201842370863744 1103 12-13 0.34410371369809667 1103 14-15 0.8112397730411445 1103 16-17 0.936232437592686 1103 18-19 0.9109135325273172 1103 20-21 0.8496210634050172 1103 22-23 0.7633334629037805 1103 24-25 0.6377339931735619 1103 26-27 0.7627412743869186 1103 28-29 0.6179148292188827 1103 30-31 0.6123724157094657 1103 32-33 0.7197598211133354 1103 34-35 0.7707882795048917 1103 36-37 0.7493605347999335 1103 38-39 0.6030531418554332 1103 40-41 0.7887558966730595 1103 42-43 0.6413140891891231 1103 44-45 0.692299274776353 1103 46-47 0.823055578568173 1103 48-49 0.9087849370020677 1103 50-51 1.0097757495360966 1103 52-53 1.147802326148117 1103 54-55 0.9746901796151164 1103 56-57 0.9510449169141637 1103 58-59 0.9362609306057834 1103 60-61 0.8660847169425452 1103 62-63 0.8247180742850801 1103 64-65 0.9723614752740204 1103 66-67 0.9695046820136639 1103 68-69 0.9905590799230879 1103 70-71 1.0331393299171232 1103 72-73 1.1880699101946561 1103 74-75 1.1132290196586325 1103 76-77 1.0919228505081477 1103 78-79 0.8323924640887306 1103 80-81 0.9245778948443863 1103 82-83 1.0134640776192256 1103 84-85 1.1075589789587994 1103 86-87 0.8519147641767653 1103 88-89 0.8021133133356031 1103 90-91 1.0891871263760642 1103 92-93 1.231860663965893 1103 94-95 1.3075960272579756 1103 96-97 1.2691256469197576 1103 98-99 1.4778006714520906 1103 100-101 1.7424924435876825 1104 1 0.27020340742735627 1104 2 0.3384101766740848 1104 3 0.5339520769406043 1104 4 0.5201225176240598 1104 5 0.46671031511206706 1104 6 0.30223582712162056 1104 7 0.4082695073213216 1104 8 0.4689712152602823 1104 9 0.5207510314301018 1104 10-11 0.4721401396169398 1104 12-13 0.5944449602368778 1104 14-15 0.5776734634994298 1104 16-17 0.49520401802379865 1104 18-19 0.6314061532450239 1104 20-21 0.5668052176800344 1104 22-23 0.3749317595496251 1104 24-25 0.6141420181778798 1104 26-27 0.31906970305571036 1104 28-29 0.21180823401434878 1104 30-31 0.33357724348906714 1104 32-33 0.263170559375844 1104 34-35 0.30345555530823276 1104 36-37 0.23648370112211126 1104 38-39 0.1704305298847828 1104 40-41 0.324288250559178 1104 42-43 0.46365046263447596 1104 44-45 0.38615980847665554 1104 46-47 0.6990790860440228 1104 48-49 0.7863693572172394 1104 50-51 0.6351585492248475 1104 52-53 0.571180174744228 1104 54-55 0.7214485696371611 1104 56-57 0.7476243730368282 1104 58-59 0.6551968171132216 1104 60-61 0.5197032302404878 1104 62-63 0.5745653258343779 1104 64-65 0.7665257416078859 1104 66-67 0.8414796591246017 1104 68-69 0.6206232249867583 1104 70-71 0.5894231876094231 1104 72-73 0.6328803628314894 1104 74-75 0.5750655853163273 1104 76-77 0.5786369852647795 1104 78-79 0.5562915145412184 1104 80-81 0.7565689917918306 1104 82-83 0.5407238725555921 1104 84-85 0.46459757185306927 1104 86-87 0.5181092570611945 1104 88-89 0.4499984205009433 1104 90-91 0.7735396493719016 1104 92-93 0.6226348432563604 1104 94-95 0.6495324876167352 1104 96-97 0.6692081960824723 1104 98-99 0.40541833418722817 1104 100-101 0.9403546152713673 1105 1 0.32948815570032863 1105 2 0.4017052436517936 1105 3 0.467185572401835 1105 4 0.45868572142890685 1105 5 0.5383154508231982 1105 6 0.3161885029272469 1105 7 0.3532132376999684 1105 8 0.49643590116210845 1105 9 0.45496581207071074 1105 10-11 0.4722057614336279 1105 12-13 0.5129643929862553 1105 14-15 0.8572097997334467 1105 16-17 0.7836001686741056 1105 18-19 0.6659975422720166 1105 20-21 0.509606460930975 1105 22-23 0.5420235918498122 1105 24-25 0.4613340128377388 1105 26-27 0.44749898620472095 1105 28-29 0.46491158098774577 1105 30-31 0.35652141192593234 1105 32-33 0.5104878873597656 1105 34-35 0.2731447947209631 1105 36-37 0.3720527248433001 1105 38-39 0.20103810968264924 1105 40-41 0.20328727014315717 1105 42-43 0.10866381298023242 1105 44-45 0.036250809856881006 1105 46-47 0.3290085447639868 1105 48-49 0.43051658649899593 1105 50-51 0.6231593175650545 1105 52-53 0.6517142233408748 1105 54-55 0.5251897954353169 1105 56-57 0.5221391192585756 1105 58-59 0.6376075627508868 1105 60-61 0.5880555229189994 1105 62-63 0.5674468797589967 1105 64-65 0.6134765434977965 1105 66-67 0.48258090619987115 1105 68-69 0.5629742417337908 1105 70-71 0.7478985711711168 1105 72-73 0.741300206868857 1105 74-75 0.678133557350133 1105 76-77 0.8062560345393734 1105 78-79 0.5936046879253567 1105 80-81 0.7544308504798103 1105 82-83 0.7124209657264018 1105 84-85 0.6997361859898348 1105 86-87 0.8904856881382948 1105 88-89 0.9337472427848965 1105 90-91 1.1075690375158196 1105 92-93 0.9707294638318906 1105 94-95 1.0274324504165548 1105 96-97 1.4453039500467284 1105 98-99 1.6296937128816076 1105 100-101 1.9992456326442003 1106 1 0.2014874097100261 1106 2 0.1865512795422184 1106 3 0.12028226103377193 1106 4 0.2500517125599089 1106 5 0.3068379755859283 1106 6 0.2747524715956544 1106 7 0.3300626719906532 1106 8 0.4854694292817996 1106 9 0.4124302739215935 1106 10-11 0.5653324968971845 1106 12-13 0.5957262148603419 1106 14-15 0.40647189099292547 1106 16-17 0.1635864921852317 1106 18-19 0.3315238798740694 1106 20-21 0.09458553175861084 1106 22-23 0.007754662345895724 1106 24-25 -0.19306297833470865 1106 26-27 -0.2707288723886663 1106 28-29 0.042349311519473076 1106 30-31 -0.0936294438907268 1106 32-33 0.14696909254854518 1106 34-35 0.3122527146513363 1106 36-37 -0.10696609068986618 1106 38-39 0.015521096959375313 1106 40-41 0.08898104112413563 1106 42-43 0.2893163472750615 1106 44-45 0.04334662816680179 1106 46-47 0.4517624922960053 1106 48-49 0.12209104047553865 1106 50-51 0.03700096040147827 1106 52-53 0.24394431990517518 1106 54-55 0.32107192896758363 1106 56-57 0.5164948737448825 1106 58-59 0.3712579419626252 1106 60-61 0.543401579531384 1106 62-63 0.34390715577541897 1106 64-65 0.07573399303984019 1106 66-67 0.0668912796094645 1106 68-69 0.21763419448774357 1106 70-71 0.020010552604244225 1106 72-73 -0.005082776263474642 1106 74-75 0.045546214318921585 1106 76-77 0.15476405393512493 1106 78-79 0.2901636004372712 1106 80-81 0.23277806752154717 1106 82-83 0.14269035111328066 1106 84-85 0.46948565758003724 1106 86-87 0.47429106755725314 1106 88-89 0.7169303419601647 1106 90-91 0.7447991340801217 1106 92-93 0.6343810863607935 1106 94-95 0.8458778895147354 1106 96-97 0.7861170794760426 1106 98-99 0.8682556093547298 1106 100-101 1.5123257437138697 1107 1 0.13109256965075744 1107 2 0.19561216645545443 1107 3 0.17368726021058123 1107 4 0.24831856129655705 1107 5 0.2688411327568758 1107 6 0.21188486939919216 1107 7 0.39530570636120643 1107 8 0.4241552326655835 1107 9 0.5678068585599263 1107 10-11 0.43518209547161746 1107 12-13 0.35599738291674754 1107 14-15 0.3378260848974648 1107 16-17 0.35948585009801093 1107 18-19 0.46622777404105165 1107 20-21 0.4367542951354295 1107 22-23 0.2871933444691379 1107 24-25 0.23655496524296638 1107 26-27 0.22005102631069207 1107 28-29 0.1673775420510566 1107 30-31 0.12821043137196853 1107 32-33 0.357586871488472 1107 34-35 0.33859397965031235 1107 36-37 0.37118512423987227 1107 38-39 0.33529815807871444 1107 40-41 0.4246508746463249 1107 42-43 0.3914719396319981 1107 44-45 0.43558424886079905 1107 46-47 0.42445257626125255 1107 48-49 0.43681062365149614 1107 50-51 0.37488914231650483 1107 52-53 0.4284367998338965 1107 54-55 0.45019421607644716 1107 56-57 0.6301290099293482 1107 58-59 0.6854448735992165 1107 60-61 0.5201212208147794 1107 62-63 0.632190952515586 1107 64-65 0.6525522232808925 1107 66-67 0.6313798857088102 1107 68-69 0.5101778477025363 1107 70-71 0.565820328212677 1107 72-73 0.45868045528499124 1107 74-75 0.4102052689108646 1107 76-77 0.5475732581537258 1107 78-79 0.603758223562096 1107 80-81 0.4985099791594969 1107 82-83 0.5280224835595071 1107 84-85 0.7025034675105957 1107 86-87 0.6720924756942317 1107 88-89 0.6039365497717419 1107 90-91 0.7469365194389823 1107 92-93 0.8895453851492512 1107 94-95 0.8764118000486469 1107 96-97 1.2660473505472538 1107 98-99 1.4174137293853342 1107 100-101 1.9151518959359954 1108 1 0.047842902402152276 1108 2 0.09939113495633123 1108 3 0.2065874629825366 1108 4 0.3748293331791146 1108 5 0.17285086258891624 1108 6 0.3391749138398765 1108 7 0.30828049377283406 1108 8 0.27282130732674403 1108 9 -0.24693166227202568 1108 10-11 0.13423710164337166 1108 12-13 0.10281092333119801 1108 14-15 0.4297250734540299 1108 16-17 0.5767595237741006 1108 18-19 0.4856618651011644 1108 20-21 0.4869444819393465 1108 22-23 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>>Per sequence quality scores pass #Quality Count 2 3001.0 3 1167.0 4 1219.0 5 1352.0 6 1470.0 7 1756.0 8 2286.0 9 2942.0 10 3743.0 11 5013.0 12 6410.0 13 7793.0 14 8505.0 15 10563.0 16 13116.0 17 16712.0 18 12963.0 19 9070.0 20 9365.0 21 9694.0 22 10502.0 23 12145.0 24 13880.0 25 16038.0 26 18640.0 27 21651.0 28 26368.0 29 30724.0 30 37430.0 31 46156.0 32 57238.0 33 73193.0 34 95897.0 35 131734.0 36 181604.0 37 232174.0 38 176339.0 39 26117.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.72962987378792 18.306519758842636 14.026111397442198 21.937738969927246 2 15.683026926328289 20.36243207767985 40.05516898496705 23.89937201102481 3 19.829235790860633 25.671602683116728 29.491375696232858 25.007785829789782 4 12.554719933516264 16.39894734083771 37.16912549179622 33.8772072338498 5 13.396675651392634 38.00486672656484 33.2654986522911 15.332958969751424 6 30.993246076434648 35.87374206301665 17.855442074997004 15.277569785551695 7 28.544395117368644 33.475196538348015 20.21486994083248 17.765538403450865 8 27.18022961887222 33.23040004673566 19.643430906499475 19.945939427892647 9 27.507116318091928 16.740351770839577 20.45252292203512 35.30000898903338 10-11 25.219317545590897 26.614111597755237 26.53595309740976 21.630617759244107 12-13 26.87838524954152 24.226723449122314 27.186497717144594 21.708393584191573 14-15 23.47588372908061 25.203652337490002 26.47345592662375 24.847008006805634 16-17 21.867764342680733 28.68506187721622 27.030047158276627 22.417126621826423 18-19 21.76456799119858 26.831039237170273 29.993581673393304 21.410811098237843 20-21 22.994025649280157 25.672900554761135 29.824437939373066 21.508635856585638 22-23 22.362461085368697 25.859565578523053 29.82175536102035 21.9562179750879 24-25 22.30417544670081 26.052378105549078 30.078384850561918 21.565061597188194 26-27 21.908344679469927 26.093506141513682 30.220005862430543 21.778143316585847 28-29 21.600426083623777 26.659645119950177 30.051134526247708 21.688794270178338 30-31 21.79870512154035 26.181261277041042 30.006313833058062 22.01371976836055 32-33 21.499193573440962 26.42069752060795 29.85571159236775 22.22439731358334 34-35 22.422679640606496 26.234685276155368 29.58723048107285 21.755404602165285 36-37 22.47015059721262 25.92029654592416 29.650437958417246 21.95911489844597 38-39 22.09448779405088 26.322899109888915 29.670144990678182 21.912468105382025 40-41 22.211463858197195 26.32921417324426 29.692835191552735 21.76648677700581 42-43 21.850092518848097 26.611968765157823 29.130749492493695 22.40718922350039 44-45 22.081604881532126 26.56956647359904 29.055842404118643 22.292986240750196 46-47 22.586086423444865 26.95853233066498 28.33569803479648 22.119683211093673 48-49 22.615724271529263 27.03997443049657 27.947594494323024 22.39670680365114 50-51 22.39906134914219 27.350931165603757 28.1198913141112 22.13011617114285 52-53 22.737610794841057 27.385576713275693 27.744828388928955 22.13198410295429 54-55 22.126515935190355 27.206742047375172 27.97737830227235 22.689363715162123 56-57 22.540700446658818 27.568587616464768 27.466335908090233 22.424376028786178 58-59 22.78718840538486 27.013980143428117 27.5918545163839 22.606976934803118 60-61 22.314448921662493 27.228106316420625 28.270700105322334 22.18674465659455 62-63 22.373206271691195 27.32324330555388 28.163288831567595 22.140261591187326 64-65 22.05520256622433 27.55539886546896 28.00956308541244 22.37983548289427 66-67 22.072301854422467 27.58163833952672 27.631152980029093 22.71490682602172 68-69 22.234635533333858 27.419407357338304 27.880471416386243 22.46548569294159 70-71 22.281639061415213 27.521515628421028 27.586375961630228 22.61046934853353 72-73 22.29915632442242 27.40246147534295 27.725325165848997 22.573057034385638 74-75 22.145661849202515 28.16191470805119 27.344031322874905 22.348392119871384 76-77 22.365932099142096 27.61742791930601 27.42736900740732 22.589270974144576 78-79 22.31697739725976 27.697359407601724 27.496681572451777 22.48898162268674 80-81 22.435901994684183 27.98557895473623 27.199398424419957 22.37912062615963 82-83 22.422409834169652 27.87620605675442 27.33077403589379 22.370610073182135 84-85 22.226879826936717 27.88841460333738 27.22404471799555 22.660660851730352 86-87 22.40455973954183 27.958720120123942 27.106594653182164 22.530125487152063 88-89 22.380037568785347 27.824554745519947 26.865798445225664 22.929609240469045 90-91 22.259068691758614 27.697981882266546 27.287052254573506 22.755897171401333 92-93 22.446561153922744 27.801337898798195 27.307074424363652 22.44502652291541 94-95 22.081514018936467 27.567961101451772 27.531091326619418 22.819433552992347 96-97 21.978016219457512 27.578018615020326 27.509707644016366 22.934257521505792 98-99 21.903611039048307 27.78584506833987 26.99277986853498 23.317764024076837 100-101 21.909863155534477 27.665850498628636 27.322513398269916 23.101772947566975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 355.0 1 272.5 2 359.5 3 503.5 4 417.0 5 415.5 6 608.0 7 1059.0 8 1681.0 9 2009.5 10 1847.5 11 1658.5 12 1734.0 13 1869.0 14 2013.0 15 2176.5 16 2242.0 17 2318.0 18 2419.0 19 2602.5 20 2886.5 21 3286.5 22 3829.5 23 4449.0 24 5259.5 25 6302.5 26 7940.0 27 9889.5 28 11595.0 29 13544.5 30 16232.5 31 19191.0 32 22800.0 33 25130.5 34 26686.5 35 28725.0 36 31337.0 37 34493.5 38 35898.0 39 36208.5 40 36662.5 41 38121.0 42 39516.0 43 40919.0 44 42795.0 45 45086.5 46 46275.5 47 48743.0 48 57144.0 49 56833.0 50 48782.0 51 45048.5 52 42989.5 53 42885.0 54 42569.5 55 40151.5 56 36821.5 57 33531.0 58 31685.0 59 28852.5 60 24240.5 61 20638.0 62 17657.5 63 13671.5 64 9826.0 65 7491.0 66 5502.5 67 4247.5 68 3182.0 69 2225.5 70 1688.5 71 1347.5 72 1114.0 73 981.0 74 785.5 75 609.5 76 452.0 77 269.0 78 177.0 79 120.0 80 80.0 81 57.0 82 39.0 83 25.0 84 17.5 85 12.0 86 11.0 87 10.5 88 6.5 89 3.0 90 2.5 91 2.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08076498437470761 2 0.03308445143060312 3 0.016093115514886132 4 0.023204027021463725 5 0.028069387525964183 6 0.03405752353150321 7 0.0839087557776156 8 0.06033047025580568 9 0.0759744755702764 10-11 0.06493384981006381 12-13 0.043863096240573364 14-15 0.04573438874230431 16-17 0.0591702689047325 18-19 0.055090851250959035 20-21 0.019835700518348022 22-23 0.014858062463743708 24-25 0.024064821572259962 26-27 0.026310372574337094 28-29 0.006512097906023691 30-31 0.0014596081513501375 32-33 0.010741218959935628 34-35 0.012649937311701193 36-37 0.004528527854188889 38-39 0.009768146859035536 40-41 0.004753082954396602 42-43 0.0023204027021463723 44-45 7.485170006923783E-5 46-47 4.865360504500458E-4 48-49 6.362394505885215E-4 50-51 0.0011227755010385673 52-53 2.6198095024233234E-4 54-55 7.485170006923783E-5 56-57 0.004191695203877317 58-59 0.0033309006530810835 60-61 0.005688729205262075 62-63 0.002657235352457943 64-65 3.368326503115702E-4 66-67 4.865360504500458E-4 68-69 0.0032560489530118453 70-71 0.0020584217519040403 72-73 0.020359662418832688 74-75 0.01571885701453994 76-77 0.02518759707329853 78-79 0.01886262841744793 80-81 0.011190329160351054 82-83 0.004603379554258126 84-85 0.0035554557532887967 86-87 0.0016093115514886132 88-89 0.0019087183517655644 90-91 0.007597447557027639 92-93 0.011152903310316436 94-95 0.014408952263328282 96-97 0.012874492411908906 98-99 0.025037893673160054 100-101 0.01957371956810569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1335975.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.64522457003066 #Duplication Level Percentage of deduplicated Percentage of total 1 81.22318937652494 48.44575370656959 2 11.190812226685875 13.349570167634479 3 3.2566710716804783 5.827346322633136 4 1.352126354754383 3.225915203055285 5 0.741389632931644 2.2110175575050253 6 0.4580071925594004 1.6390765112936831 7 0.30289966597764817 1.264656301959687 8 0.21947763776409016 1.0472634394031202 9 0.15925936550126413 0.8549154558183144 >10 0.9737473681167705 10.976213187668417 >50 0.07700402898076625 3.132498434360819 >100 0.04062218626890147 4.494955351369856 >500 0.003532341660709904 1.4911394479276827 >1k 0.001009240474488544 1.1644988694620182 >5k 2.52310118622136E-4 0.8751800433388947 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6489 0.4857126817492842 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5142 0.3848874417560209 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3389 0.253672411534647 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3149 0.2357080035180299 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2032 0.15209865454069127 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1469 0.10995714740171036 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1445 0.10816070660004866 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1373 0.10277138419506353 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 7.485170006923783E-5 0.0 0.0 0.0 7.485170006923783E-5 14-15 7.485170006923783E-5 0.0 0.0 0.0 7.485170006923783E-5 16-17 7.485170006923783E-5 0.0 0.0 0.0 7.485170006923783E-5 18-19 1.1227755010385674E-4 0.0 0.0 0.0 7.485170006923783E-5 20-21 1.4970340013847566E-4 0.0 0.0 1.1227755010385674E-4 1.4970340013847566E-4 22-23 2.2455510020771347E-4 0.0 0.0 2.619809502423324E-4 1.4970340013847566E-4 24-25 2.2455510020771347E-4 0.0 0.0 4.4911020041542694E-4 1.4970340013847566E-4 26-27 2.2455510020771347E-4 0.0 0.0 5.613877505192837E-4 1.4970340013847566E-4 28-29 4.11684350380808E-4 0.0 0.0 0.0012350530511424241 1.4970340013847566E-4 30-31 5.239619004846648E-4 0.0 0.0 0.0024701061022848483 1.4970340013847566E-4 32-33 5.239619004846648E-4 0.0 0.0 0.004266546903946556 2.2455510020771347E-4 34-35 5.239619004846648E-4 0.0 0.0 0.006399820355919834 2.2455510020771347E-4 36-37 5.239619004846648E-4 0.0 0.0 0.009805572709070155 2.2455510020771347E-4 38-39 5.239619004846648E-4 0.0 0.0 0.0144463781133629 2.2455510020771347E-4 40-41 5.239619004846648E-4 0.0 0.0 0.02028481071876345 2.2455510020771347E-4 42-43 5.613877505192837E-4 0.0 0.0 0.028331368476206514 2.2455510020771347E-4 44-45 5.988136005539026E-4 0.0 0.0 0.0364153520836842 2.2455510020771347E-4 46-47 5.988136005539026E-4 0.0 0.0 0.048391624094762255 2.2455510020771347E-4 48-49 6.736653006231404E-4 0.0 0.0 0.06560751511068695 2.2455510020771347E-4 50-51 6.736653006231404E-4 7.485170006923783E-5 0.0 0.0748142742192032 2.2455510020771347E-4 52-53 6.736653006231404E-4 7.485170006923783E-5 0.0 0.08630401017983122 2.619809502423324E-4 54-55 6.736653006231404E-4 7.485170006923783E-5 0.0 0.10576545219783304 2.994068002769513E-4 56-57 7.485170006923782E-4 7.485170006923783E-5 0.0 0.13780197982746684 2.994068002769513E-4 58-59 7.485170006923782E-4 7.485170006923783E-5 0.0 0.17859615636520143 2.994068002769513E-4 60-61 7.485170006923782E-4 7.485170006923783E-5 0.0 0.22687550290985983 2.994068002769513E-4 62-63 7.485170006923782E-4 7.485170006923783E-5 0.0 0.27698871610621456 3.742585003461891E-4 64-65 7.485170006923782E-4 7.485170006923783E-5 0.0 0.33204214150713895 4.11684350380808E-4 66-67 7.485170006923782E-4 7.485170006923783E-5 0.0 0.4049851232246112 4.4911020041542694E-4 68-69 7.485170006923782E-4 7.485170006923783E-5 0.0 0.4968281592095661 4.4911020041542694E-4 70-71 7.485170006923782E-4 1.4970340013847566E-4 0.0 0.5527423791612867 4.4911020041542694E-4 72-73 7.485170006923782E-4 1.4970340013847566E-4 0.0 0.6239263459271318 4.8653605045004587E-4 74-75 7.485170006923782E-4 1.4970340013847566E-4 0.0 0.699825969797339 5.613877505192837E-4 76-77 7.485170006923782E-4 1.4970340013847566E-4 0.0 0.7817511555231198 5.988136005539026E-4 78-79 7.485170006923782E-4 1.4970340013847566E-4 0.0 0.8687288310035741 5.988136005539026E-4 80-81 8.23368700761616E-4 1.4970340013847566E-4 0.0 1.0009917850259173 6.362394505885215E-4 82-83 8.23368700761616E-4 1.4970340013847566E-4 0.0 1.152566477666124 7.485170006923782E-4 84-85 8.23368700761616E-4 1.4970340013847566E-4 0.0 1.373528696270514 7.485170006923782E-4 86-87 8.60794550796235E-4 1.4970340013847566E-4 0.0 1.6865959318101011 7.485170006923782E-4 88-89 9.356462508654728E-4 1.4970340013847566E-4 0.0 2.0168416325155785 7.485170006923782E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1560 0.0 30.77466 2 GTACCTG 1575 0.0 30.481571 1 GTATCAA 12870 0.0 23.932772 1 GAGTACT 6130 0.0 23.571392 12-13 GTACATG 8330 0.0 23.50979 1 TACATGG 8530 0.0 22.568491 2 ACATGGG 8385 0.0 22.336864 3 GTACTTT 6595 0.0 21.69077 14-15 ATCGCCG 55 0.0028764023 21.593431 24-25 GTCTACG 135 3.7785497E-4 21.12584 1 GTGTAGC 635 0.0 20.959496 1 GGTATCA 9650 0.0 20.934286 1 AGTACTT 6370 0.0 20.705975 12-13 GTACCCG 185 6.5139775E-6 20.554873 1 CATGGGA 4940 0.0 20.30414 4 TCAACGC 15100 0.0 20.179487 4 GTATAGG 425 0.0 20.131685 1 TATCAAC 15220 0.0 20.112577 2 ATCAACG 15120 0.0 20.089918 3 CAACGCA 15230 0.0 20.039204 5 >>END_MODULE