FastQCFastQC Report
Thu 26 May 2016
SRR936476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335975
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83380.624113475177305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75680.5664776661239919No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.35157843522521004No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40520.30329908868055167No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20800.15569153614401468No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19130.14319130223245197No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15300.11452310110593386No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15110.11310091880461835No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13820.10344504949568667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC352.7896764E-454.279533
TACCTGG13200.030.2317522
GTACCTG14150.028.5410021
TATAGCG950.001228545224.9934125
GTATCAA142350.023.4307611
GTACATG82150.023.1344491
GAGTACT85100.023.04880312-13
GGTATCA107100.023.0242061
GTACACG2503.8198777E-1022.8059431
TACATGG83800.022.7330822
GTAAGGT5100.022.3470524
ACATGGG81250.021.9790343
TAAGGTG6300.021.1055475
GTACTTT92700.021.0302814-15
AGTACTT87700.020.90333612-13
CATGGGG31750.020.7897954
TCAACGC166850.020.207444
ATCAACG167250.020.1053353
CAACGCA168450.020.0155015
TATCAAC169450.019.8495032
Produced by FastQC (version 0.11.5)