FastQCFastQC Report
Thu 26 May 2016
SRR936475_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936475_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755395
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75820.43192557800381115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64400.3668689952973547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35660.203145161060616No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24480.13945579200123048No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21570.12287832653049599No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18630.10612995935387762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15550.027.8040352
GTACATG94400.026.7243371
GTACCTG16850.026.5040591
GTATCAA130600.025.9376581
TACATGG98000.025.5009572
GGTATCA94800.025.2083321
ACATGGG96700.024.5178993
GAGTACT71250.023.57327512-13
ATCAACG153250.022.1053943
GTACTTT76200.022.07311814-15
TCAACGC153550.022.0634634
TATCAAC153850.022.0494372
CAACGCA154500.021.7746495
AACGCAG157350.021.3500546
CATGGGG38400.021.1591824
GTATAGG5650.021.0220951
AGTACTT74850.020.56688912-13
CATGGGA56650.020.130074
TATAACG1456.137976E-419.6598432
ACGCAGA171800.019.470237
Produced by FastQC (version 0.11.5)