FastQCFastQC Report
Thu 26 May 2016
SRR936475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936475_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755395
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93300.5315043052988074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87990.501254703357364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52220.2974829027085072No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.13877218517769505No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23680.13489841317766085No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23190.13210701864822447No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22550.12846111558936876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15000.028.1853282
GGTATCA110750.026.6836511
GTATCAA148450.024.259821
GTACATG97700.023.9259471
TACACCG1201.7124797E-423.7476225
GTACCTG18500.023.6273331
TACATGG100500.022.6881712
GAGTACT95000.022.12467612-13
ACATGGG100700.021.838683
GTGTACG1553.9006518E-521.45681
GTACTTT99150.021.07946414-15
CCTACAC8050.020.6512813
AGTACTT99750.020.07113312-13
ATCAACG179000.020.0075173
CATGGGA59200.019.9774534
TATCAAC179600.019.9164962
TCAACGC180200.019.8737144
TACCGTG1200.00476881519.7885577
CAACGCA181700.019.7613685
GTAAGGT6850.019.4146674
Produced by FastQC (version 0.11.5)