##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936475_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1755395 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.913869527941003 31.0 31.0 34.0 30.0 34.0 2 32.017116945188974 33.0 31.0 34.0 30.0 34.0 3 32.13550397488884 33.0 31.0 34.0 30.0 34.0 4 35.8779909934801 37.0 35.0 37.0 35.0 37.0 5 35.750440783983095 37.0 35.0 37.0 35.0 37.0 6 35.80452946487828 37.0 35.0 37.0 35.0 37.0 7 35.728265148299954 37.0 35.0 37.0 33.0 37.0 8 35.69951435431911 37.0 35.0 37.0 33.0 37.0 9 37.30451436856092 39.0 37.0 39.0 34.0 39.0 10-11 37.207063082668 39.0 37.0 39.0 33.0 39.0 12-13 37.07633153791597 39.0 37.0 39.0 33.0 39.0 14-15 38.14869388371278 40.0 37.5 41.0 33.0 41.0 16-17 38.13220072975028 40.0 37.5 41.0 33.0 41.0 18-19 38.303418034117676 40.0 38.0 41.0 33.5 41.0 20-21 38.3257548870767 40.0 38.0 41.0 34.0 41.0 22-23 38.23042050364732 40.0 38.0 41.0 33.5 41.0 24-25 38.11449417367601 40.0 37.0 41.0 33.0 41.0 26-27 37.90875842759037 40.0 37.0 41.0 33.0 41.0 28-29 37.90297055648444 40.0 37.0 41.0 33.0 41.0 30-31 37.750266749079266 40.0 37.0 41.0 33.0 41.0 32-33 37.53594148325591 40.0 36.0 41.0 32.5 41.0 34-35 37.34680883789689 39.0 36.0 41.0 32.0 41.0 36-37 37.14778554114601 39.0 36.0 41.0 31.0 41.0 38-39 36.938503869499456 39.0 35.5 41.0 31.0 41.0 40-41 36.69131933268581 39.0 35.0 41.0 30.5 41.0 42-43 36.40676201082947 39.0 35.0 40.0 30.0 41.0 44-45 36.08565080793782 39.0 35.0 40.0 30.0 41.0 46-47 35.64675016164453 38.5 35.0 40.0 28.5 41.0 48-49 35.196206266965554 38.0 34.5 40.0 26.5 41.0 50-51 34.87263407950917 38.0 34.0 40.0 26.0 41.0 52-53 34.58501676260899 38.0 34.0 40.0 24.5 41.0 54-55 34.2431051130942 38.0 33.5 40.0 24.0 41.0 56-57 33.91959530476046 37.5 33.0 40.0 23.0 41.0 58-59 33.54773768866836 37.0 33.0 40.0 22.5 41.0 60-61 33.17934538949923 36.5 32.0 39.0 22.0 40.5 62-63 32.83897214017358 36.0 32.0 39.0 21.0 40.0 64-65 32.50179760110972 36.0 31.5 39.0 19.5 40.0 66-67 32.05020465479279 35.0 31.0 38.5 18.0 40.0 68-69 31.613789204139238 35.0 31.0 38.0 17.5 40.0 70-71 31.11201410508746 35.0 30.0 37.0 13.5 39.5 72-73 30.551191896980452 34.0 29.5 36.5 9.0 39.0 74-75 30.010241569561266 34.0 29.0 36.0 7.5 39.0 76-77 28.642782963378615 32.5 27.0 34.5 7.0 36.5 78-79 29.409820866528616 34.0 29.0 35.0 4.5 37.0 80-81 29.249712457879852 34.0 29.0 35.0 2.0 37.0 82-83 28.84567604442305 34.0 29.0 35.0 2.0 36.5 84-85 28.39617265629673 33.0 27.5 35.0 2.0 36.0 86-87 27.95723640548139 33.0 27.0 35.0 2.0 35.5 88-89 27.443900375698917 33.0 26.0 35.0 2.0 35.0 90-91 26.862052415553194 32.0 25.5 34.0 2.0 35.0 92-93 26.27223929656858 32.0 25.0 34.0 2.0 35.0 94-95 25.524093722495508 31.5 23.0 34.0 2.0 35.0 96-97 24.679633928545996 31.0 19.0 34.0 2.0 35.0 98-99 23.5272246417473 31.0 2.0 34.0 2.0 35.0 100-101 19.695579627377313 26.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33836568820986557 1101 2 0.4892437980765436 1101 3 0.43867573689212946 1101 4 0.2529984954783089 1101 5 0.3126773493486894 1101 6 0.33874060962170205 1101 7 0.34376291650863067 1101 8 0.2829152482249171 1101 9 0.35169777520454915 1101 10-11 0.4225037926981372 1101 12-13 0.5665513632770072 1101 14-15 0.49212444027450175 1101 16-17 0.5920673261393219 1101 18-19 0.6336838257292285 1101 20-21 0.45628054662571316 1101 22-23 0.5191733291948708 1101 24-25 0.536440419835273 1101 26-27 0.4360065609003456 1101 28-29 0.5233923929010871 1101 30-31 0.6968977040930397 1101 32-33 0.6985716751587105 1101 34-35 0.619587868317474 1101 36-37 0.6712638632600729 1101 38-39 0.5627487432405545 1101 40-41 0.6434769788082519 1101 42-43 0.6743408631902668 1101 44-45 0.564120827530914 1101 46-47 0.4911643199163862 1101 48-49 0.5768934900985627 1101 50-51 0.605027288088678 1101 52-53 0.39616446676454586 1101 54-55 0.5176788116937203 1101 56-57 0.5018165884258252 1101 58-59 0.6556121004993045 1101 60-61 0.7576282662010811 1101 62-63 0.7416460663685314 1101 64-65 0.6895693632720707 1101 66-67 0.6845226714897095 1101 68-69 0.62758128250141 1101 70-71 0.5722730598891452 1101 72-73 0.6738361409525879 1101 74-75 0.8185758554139859 1101 76-77 0.6838699403587221 1101 78-79 0.7283698395475575 1101 80-81 0.7742261318366168 1101 82-83 0.9052679723266941 1101 84-85 0.8238930542106111 1101 86-87 0.7632947454546581 1101 88-89 0.5789675631909752 1101 90-91 0.7030911398314075 1101 92-93 0.8526702969737343 1101 94-95 0.9199816086989614 1101 96-97 0.9956033340975772 1101 98-99 0.5809151802624832 1101 100-101 0.8392378473499171 1102 1 0.2626894143558438 1102 2 0.40256516341197823 1102 3 0.32755779473198743 1102 4 0.2286428000228682 1102 5 0.17148858287220037 1102 6 0.23703394033191216 1102 7 0.26688410478327285 1102 8 0.18168320022176943 1102 9 0.315299486792199 1102 10-11 0.3848882113485388 1102 12-13 0.4044313062240832 1102 14-15 0.4212853688599907 1102 16-17 0.47987595293452046 1102 18-19 0.48736644426158904 1102 20-21 0.46038737330287205 1102 22-23 0.3288305198303263 1102 24-25 0.3119654542637633 1102 26-27 0.27209745210632974 1102 28-29 0.3797260049412188 1102 30-31 0.6156772142248528 1102 32-33 0.4746524837363211 1102 34-35 0.49367059505365063 1102 36-37 0.3994607942063624 1102 38-39 0.3963300436536912 1102 40-41 0.2351182339725142 1102 42-43 0.3209176879000779 1102 44-45 0.3721770935155675 1102 46-47 0.37704249225555486 1102 48-49 0.2478887192939183 1102 50-51 0.342711726412503 1102 52-53 0.32921335521625394 1102 54-55 0.25080270506378355 1102 56-57 0.5250287662735502 1102 58-59 0.37917594075112504 1102 60-61 0.2404964050954277 1102 62-63 0.17289483677971162 1102 64-65 0.31923688245365867 1102 66-67 0.37315635238682177 1102 68-69 0.4368782036109877 1102 70-71 0.149209662289298 1102 72-73 0.10879581043911202 1102 74-75 0.06592363231738929 1102 76-77 0.31032085517280805 1102 78-79 0.38101345552237476 1102 80-81 0.3791949002804458 1102 82-83 0.38825661092594643 1102 84-85 0.3842363554106889 1102 86-87 0.4000139565504668 1102 88-89 0.08533314454057006 1102 90-91 0.1503774372935318 1102 92-93 0.36556253580906883 1102 94-95 0.4840542528067715 1102 96-97 0.5510774635486904 1102 98-99 0.419154163146608 1102 100-101 0.5258623801061653 1103 1 0.2936011874425368 1103 2 0.3440075327516112 1103 3 0.27631916295558057 1103 4 0.1855011335540837 1103 5 0.23822955359382547 1103 6 0.2131956956093788 1103 7 0.1767640705895559 1103 8 0.3193114266601569 1103 9 0.18568125726687157 1103 10-11 0.4099431231335018 1103 12-13 0.26923389093664696 1103 14-15 0.3910589542136833 1103 16-17 0.36848561622138476 1103 18-19 0.2840068818936743 1103 20-21 0.25590479072741346 1103 22-23 0.3158612466842641 1103 24-25 0.33298345939281404 1103 26-27 0.32963372356644527 1103 28-29 0.41478213369006056 1103 30-31 0.5315085786491451 1103 32-33 0.5364622277583564 1103 34-35 0.4410437339893676 1103 36-37 0.4656164349823726 1103 38-39 0.4682667081041032 1103 40-41 0.5699406166097063 1103 42-43 0.6949176997729012 1103 44-45 0.5419174349778189 1103 46-47 0.5703294523391449 1103 48-49 0.6207847074685873 1103 50-51 0.7270832387721029 1103 52-53 0.5714900868668096 1103 54-55 0.7433502853833929 1103 56-57 0.5733603384721562 1103 58-59 0.40308631284525376 1103 60-61 0.14859233153144658 1103 62-63 0.34049807093543194 1103 64-65 0.5126931786092328 1103 66-67 0.2989821069044254 1103 68-69 0.4973754075622665 1103 70-71 0.24915341481227316 1103 72-73 0.47614912176815594 1103 74-75 0.36382704100354246 1103 76-77 0.37438658309530126 1103 78-79 0.3405387208798665 1103 80-81 0.5154534487096747 1103 82-83 0.47776207835880413 1103 84-85 0.6465802278491317 1103 86-87 0.5753812718687783 1103 88-89 0.4895006521985401 1103 90-91 0.4185162839679535 1103 92-93 0.420532942809281 1103 94-95 0.40914433781615145 1103 96-97 0.5695046712992671 1103 98-99 0.5796193104882867 1103 100-101 0.5754628597680274 1104 1 0.2585257420597564 1104 2 0.24030770742922414 1104 3 0.23092795882350003 1104 4 0.17233366120384375 1104 5 0.12619124187327913 1104 6 0.16484565430963727 1104 7 0.1406983340322867 1104 8 0.1448868773088421 1104 9 0.16915473891820199 1104 10-11 0.17548439053167897 1104 12-13 0.22805164997782867 1104 14-15 0.2528669954020941 1104 16-17 0.2798213056713692 1104 18-19 0.23329335167564835 1104 20-21 0.2177984404965443 1104 22-23 0.08590621751866934 1104 24-25 0.09740158433921664 1104 26-27 0.35729627831305777 1104 28-29 0.18907368130965096 1104 30-31 0.15887774722282444 1104 32-33 0.3002625867426687 1104 34-35 0.2362176066718078 1104 36-37 0.30813997734207277 1104 38-39 0.3692961174349847 1104 40-41 0.35372824714774964 1104 42-43 0.19440103919964002 1104 44-45 0.26029243667044 1104 46-47 0.28455461945642213 1104 48-49 0.37867010029763293 1104 50-51 0.524444568669054 1104 52-53 0.4669064921188735 1104 54-55 0.3163592088366869 1104 56-57 0.42667417290907395 1104 58-59 0.3259465120770315 1104 60-61 0.22767675603566317 1104 62-63 0.4616330090159053 1104 64-65 0.3026750187574052 1104 66-67 0.42474336879597274 1104 68-69 0.49122528110530084 1104 70-71 0.33760423050106425 1104 72-73 0.4341461517973606 1104 74-75 0.5536205121832403 1104 76-77 0.4359167349188837 1104 78-79 0.5421869493539724 1104 80-81 0.5712815274214229 1104 82-83 0.5748261102671854 1104 84-85 0.484192937037232 1104 86-87 0.46529946978558456 1104 88-89 0.3696164419414565 1104 90-91 0.42105730913447204 1104 92-93 0.6063810470080178 1104 94-95 0.41928526849727277 1104 96-97 0.6903186900428828 1104 98-99 0.6348439164104711 1104 100-101 0.4216972106247887 1105 1 0.18265414225679066 1105 2 0.2600502867443524 1105 3 0.1614490031013176 1105 4 0.08798870653981083 1105 5 0.20157664314669432 1105 6 0.06545317442347454 1105 7 0.11620505689000282 1105 8 0.09276511787221153 1105 9 0.2808827961309035 1105 10-11 0.07844445475002004 1105 12-13 0.026040265817137254 1105 14-15 0.22180365276438607 1105 16-17 0.2669890396031036 1105 18-19 0.25101998586827534 1105 20-21 0.13426442484332313 1105 22-23 0.21450572858356054 1105 24-25 0.11626709069719965 1105 26-27 0.23884809996120993 1105 28-29 0.15641582642120966 1105 30-31 0.23242006382654523 1105 32-33 0.28482920415094526 1105 34-35 0.2647906641581699 1105 36-37 0.37350220892946595 1105 38-39 0.27933525048736385 1105 40-41 0.26471560416159434 1105 42-43 0.3436202025367976 1105 44-45 0.20626145861778156 1105 46-47 0.27537066674447175 1105 48-49 0.4067145423095653 1105 50-51 0.49895152869678583 1105 52-53 0.40856358076572974 1105 54-55 0.43849766139356205 1105 56-57 0.5702742312893818 1105 58-59 0.442539747258472 1105 60-61 0.3297018230818054 1105 62-63 0.4693556295621235 1105 64-65 0.26212487554320063 1105 66-67 0.21340253724141434 1105 68-69 0.0910706644997532 1105 70-71 0.07209513594320427 1105 72-73 0.2495530808105464 1105 74-75 0.143456864873297 1105 76-77 0.18884806507778862 1105 78-79 0.12273171063322863 1105 80-81 0.28627751377502264 1105 82-83 0.20029771371892124 1105 84-85 0.10050111210518864 1105 86-87 0.13466759413564944 1105 88-89 0.20986464948346395 1105 90-91 0.3501224944007433 1105 92-93 0.4216529715514632 1105 94-95 0.6361279882900206 1105 96-97 0.6713697180836711 1105 98-99 0.4765019172496885 1105 100-101 0.5284633974782729 1106 1 0.14941646749594284 1106 2 0.23356757887091106 1106 3 0.2089949927996244 1106 4 0.14057516717262786 1106 5 0.13896442828282574 1106 6 0.14845906110485885 1106 7 0.12852661686056877 1106 8 0.17809624370002552 1106 9 0.2581859602221499 1106 10-11 0.215661158208448 1106 12-13 0.2793165719245678 1106 14-15 0.36957039301457684 1106 16-17 0.38551001090552717 1106 18-19 0.21112852155627593 1106 20-21 0.22456611726422437 1106 22-23 0.19930153432307662 1106 24-25 0.2468024107854987 1106 26-27 0.19444044727541154 1106 28-29 0.2777513672600591 1106 30-31 0.18126296301712586 1106 32-33 0.06285900547545253 1106 34-35 0.08031310713094086 1106 36-37 0.22685209855418265 1106 38-39 0.20899308713195808 1106 40-41 0.05375349967300025 1106 42-43 0.27241527244114394 1106 44-45 0.24916984713875934 1106 46-47 0.16666205747294782 1106 48-49 0.22566045842435045 1106 50-51 -0.013314843635811258 1106 52-53 -0.10675282835862987 1106 54-55 0.0075116422618464185 1106 56-57 0.04446113342330449 1106 58-59 0.026410240268035068 1106 60-61 -0.017408184276547445 1106 62-63 -0.042829800901451165 1106 64-65 -0.018927368754070528 1106 66-67 -0.19678326113974975 1106 68-69 0.019335933079588585 1106 70-71 -0.11712487877349886 1106 72-73 0.06086102783041625 1106 74-75 0.0644079318342996 1106 76-77 -0.07599556994796686 1106 78-79 -0.17684886331820238 1106 80-81 -0.180738674598782 1106 82-83 0.05164429208536703 1106 84-85 0.17405519599040176 1106 86-87 0.07723014012534435 1106 88-89 0.1319213087917781 1106 90-91 0.30485207497469347 1106 92-93 0.36602751165447955 1106 94-95 0.5148936247874474 1106 96-97 0.6677199756259853 1106 98-99 0.6967447428567546 1106 100-101 0.6740870177873255 1107 1 -0.05843669178578992 1107 2 0.04699038943657996 1107 3 -0.05658660921055514 1107 4 0.005268738294766706 1107 5 -0.1662896012569135 1107 6 -0.00948755898461684 1107 7 0.016454238359614237 1107 8 0.010691389866600787 1107 9 -0.186627118162356 1107 10-11 -0.038373437611866734 1107 12-13 -0.011530857137145745 1107 14-15 0.007036226194692574 1107 16-17 -0.11878213838661367 1107 18-19 -0.0347262617985038 1107 20-21 -0.0011254927131076897 1107 22-23 0.06449504415944318 1107 24-25 0.07696926130949322 1107 26-27 -0.0513737342530618 1107 28-29 3.6195123129800777E-6 1107 30-31 0.002969925081231395 1107 32-33 0.0336822266558201 1107 34-35 -0.0535325752861624 1107 36-37 0.05218717853212951 1107 38-39 0.06243489628805321 1107 40-41 0.032790240495458534 1107 42-43 -0.0580773119800142 1107 44-45 -0.09258644997468934 1107 46-47 -0.07457070590267278 1107 48-49 -0.03792613837653391 1107 50-51 -0.15654215257740134 1107 52-53 -0.03602322120045187 1107 54-55 -0.16228948539642118 1107 56-57 -0.07812328773253796 1107 58-59 -0.12118827661619491 1107 60-61 -0.18935967658518393 1107 62-63 -0.34991566227302684 1107 64-65 -0.3006442897567112 1107 66-67 -0.263599924742131 1107 68-69 -0.20553153643073685 1107 70-71 -0.22255479848913495 1107 72-73 -0.2335253483416757 1107 74-75 -0.31334640413432524 1107 76-77 -0.40980432768529695 1107 78-79 -0.2727801006780126 1107 80-81 -0.1641210365525687 1107 82-83 -0.041057491330704465 1107 84-85 -0.20867324673804077 1107 86-87 -0.18697299157778602 1107 88-89 -0.11676717561099181 1107 90-91 0.027865956202859365 1107 92-93 -0.30848465357196986 1107 94-95 -0.3226088266435738 1107 96-97 -0.2392034784403272 1107 98-99 0.055356620040058147 1107 100-101 0.14635627684229746 1108 1 0.044626201725847636 1108 2 0.1565198683361757 1108 3 -0.0016694324325285947 1108 4 0.036571004508523686 1108 5 0.06583922815474352 1108 6 0.06418592629858466 1108 7 0.010910542573022042 1108 8 0.13401843390175827 1108 9 0.04045141011007303 1108 10-11 -0.009512552966711496 1108 12-13 0.02832633817496344 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2316 88-89 -2.118103151551889 2316 90-91 -2.536369260560072 2316 92-93 -2.746039693139746 2316 94-95 -3.0011583228674183 2316 96-97 -2.442194413171176 2316 98-99 -1.8587661725716096 2316 100-101 -1.4672994250671998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 21.0 5 65.0 6 131.0 7 355.0 8 782.0 9 1378.0 10 2443.0 11 3862.0 12 5619.0 13 7606.0 14 9877.0 15 13321.0 16 17185.0 17 22212.0 18 19767.0 19 12997.0 20 11712.0 21 12290.0 22 13167.0 23 14715.0 24 16935.0 25 20497.0 26 24819.0 27 30214.0 28 37390.0 29 45632.0 30 56398.0 31 69145.0 32 86161.0 33 109345.0 34 143809.0 35 193654.0 36 266740.0 37 320409.0 38 158972.0 39 5760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60428811697516 19.314815152440552 14.830706436277616 22.25019029430667 2 15.857660490873043 20.925058681433878 39.254631845217745 23.962648982475333 3 19.568722312362134 25.021363918108396 30.20550380106831 25.20440996846116 4 13.007209802049461 17.065514860532257 36.654289535988624 33.272985801429655 5 13.225465916710677 38.03138261599279 33.905143442471186 14.838008024825344 6 31.5433848222195 36.38787851167401 17.05650295232697 15.01223371377952 7 28.445636706061432 33.55827808457816 20.200137405049805 17.7959478043106 8 27.154419300461385 34.142627397161945 19.325128734496744 19.377824567879927 9 26.92801335311995 16.300832576143833 20.207531638178303 36.563622432557914 10-11 25.192278660928167 26.777249564912744 26.621529627234896 21.408942146924197 12-13 26.641639061293898 24.12548172918346 27.560947820860832 21.67193138866181 14-15 23.115842831238922 25.286788611286287 26.47148463558178 25.125883921893006 16-17 21.75358209609947 28.80434813570349 27.231364868198348 22.21070489999869 18-19 21.638826785646636 26.603946585483197 30.385010984891753 21.37221564397842 20-21 22.48538069996041 25.631755996547767 30.535495062336754 21.347368241155074 22-23 22.121978591652 25.731917123830033 30.44443115205168 21.70167313246629 24-25 22.058389989896813 25.90438060222065 30.68210334305294 21.355126064829598 26-27 21.87823467451448 26.087833322794385 30.511173281424732 21.522758721266406 28-29 21.516366126351123 26.169765875923012 30.88537388187495 21.428494115850917 30-31 21.633487810366386 26.01101752606978 30.70676803094478 21.648726632619056 32-33 21.364415807853792 26.25053408760746 30.53260640449374 21.852443700045004 34-35 22.137030158712072 26.00234707015005 30.348642459182628 21.511980311955245 36-37 22.25237727387699 25.740583762302236 30.24586811228617 21.761170851534605 38-39 21.941804819688944 25.883524183900185 30.47376516834729 21.70090582806358 40-41 21.97716183537039 26.002552132141197 30.465963501092347 21.554322531396068 42-43 21.87360516861313 26.195528051296467 29.980041182329938 21.950825597760463 44-45 21.923714768983434 26.359947291271556 29.658088300223266 22.058249639521748 46-47 22.360921615932597 26.58908678673461 28.994072559167595 22.0559190381652 48-49 22.548622504785342 26.831960395588368 28.54123940388296 22.078177695743324 50-51 22.478428441501364 27.00699592708923 28.39011643357828 22.124459197831122 52-53 22.64358229837637 27.136238333769136 28.332014535826005 21.888164832028494 54-55 22.182740740825135 27.033759942212406 28.398211455661805 22.385287861300654 56-57 22.515160630901025 27.324459151186986 27.953303433674947 22.207076784237046 58-59 22.666861877562322 26.812976093596408 28.06992158047906 22.45024044836221 60-61 22.350120218292837 26.91664931493661 28.639156270108185 22.094074196662373 62-63 22.582990010208544 26.80637715108648 28.65741669097273 21.953216147732242 64-65 22.341609754874817 26.918878763641167 28.5213245464717 22.218186935012316 66-67 22.217088779940912 27.205940158621466 28.185987715438422 22.3909833459992 68-69 22.33369935355417 27.05826557686638 28.382062445613244 22.225972623966207 70-71 22.26159451519905 27.079481360558322 28.248012403411675 22.41091172083095 72-73 22.330253335714616 27.134628054611063 28.000861362508 22.53425724716632 74-75 22.28213682634526 27.55016874839057 27.73361568209216 22.434078743172016 76-77 22.45392300343459 27.205466404082994 28.00777268928555 22.332837903196864 78-79 22.38546540924754 27.06472468770803 27.99354636004281 22.55626354300162 80-81 22.339466762525827 27.52278316669069 27.757009558761116 22.380740512022367 82-83 22.427047712910728 27.425530914906922 27.716556740351244 22.43086463183111 84-85 22.33385810712848 27.33254925842826 27.541053556265826 22.792539078177434 86-87 22.463422595701118 27.22223076734646 27.634561495556248 22.679785141396174 88-89 22.503125445958197 27.22691407038883 27.523721059538286 22.746239424114684 90-91 22.28586550384428 27.24442030126998 27.76069429794114 22.7090198969446 92-93 22.268958787066033 27.12381084765576 27.815782292617925 22.791448072660277 94-95 22.082769118411928 26.943486600405954 27.796744767114294 23.176999514067823 96-97 22.199973339318692 26.803982467753677 27.744967796403543 23.251076396524084 98-99 22.00918886739946 26.93773730549139 27.500206507404357 23.55286731970479 100-101 21.843605176131458 27.054173918244924 27.62755894073312 23.47466196489049 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 586.0 1 466.5 2 495.0 3 628.0 4 508.0 5 442.5 6 753.5 7 1497.0 8 2293.0 9 2788.5 10 2671.0 11 2314.5 12 2276.0 13 2410.0 14 2587.0 15 2787.0 16 2980.0 17 3148.5 18 3424.0 19 3675.0 20 3965.0 21 4422.0 22 5043.5 23 5928.0 24 6948.5 25 8270.5 26 10259.5 27 12835.5 28 15269.5 29 17836.5 30 21182.0 31 24536.5 32 28786.0 33 32044.5 34 34749.0 35 37956.5 36 42007.5 37 46452.0 38 48269.5 39 48888.5 40 50032.0 41 52375.0 42 54308.5 43 56309.5 44 59456.0 45 62013.5 46 61969.5 47 63793.0 48 73569.0 49 73287.0 50 63842.0 51 59344.0 52 55752.0 53 55024.0 54 54765.0 55 51590.0 56 47435.0 57 43474.5 58 39817.0 59 35279.5 60 29979.0 61 25413.5 62 21490.0 63 17123.0 64 12760.0 65 9863.5 66 7471.0 67 5776.5 68 4340.0 69 2996.5 70 2281.0 71 1829.5 72 1498.5 73 1315.0 74 1098.5 75 887.5 76 629.5 77 392.5 78 267.0 79 173.5 80 102.0 81 67.0 82 46.5 83 22.0 84 18.0 85 13.5 86 5.5 87 3.0 88 2.0 89 2.0 90 2.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012475824529521845 2 0.008829921470666148 3 0.005639756294167409 4 3.9877064706234214E-4 5 0.0052409856471050675 6 0.0 7 5.696723529462031E-5 8 0.002164754941195571 9 0.0 10-11 0.0 12-13 0.0 14-15 4.5573788235696246E-4 16-17 0.0038737720000341803 18-19 0.0016805334411912989 20-21 5.69672352946203E-4 22-23 2.848361764731015E-4 24-25 4.8422150000427253E-4 26-27 1.1393447058924061E-4 28-29 5.696723529462031E-5 30-31 5.69672352946203E-4 32-33 0.0037028702941503196 34-35 8.545085294193044E-4 36-37 0.0013102464117762669 38-39 0.005411887352988929 40-41 0.0 42-43 0.001737500676485919 44-45 0.005013116705926586 46-47 0.0 48-49 0.0019938532353117105 50-51 9.399593823612349E-4 52-53 4.5573788235696246E-4 54-55 5.696723529462031E-5 56-57 0.010880741941272478 58-59 0.0016235662058966785 60-61 0.002648976441199844 62-63 1.7090170588386088E-4 64-65 0.002677460058847154 66-67 0.0011963119411870264 68-69 0.0015665989706020581 70-71 0.004073157323565351 72-73 0.002079304088253641 74-75 0.006779101000059816 76-77 0.0010254102353031654 78-79 0.006067010558877062 80-81 0.0023926238823740527 82-83 0.0029907798529675658 84-85 0.0020223368529590207 86-87 4.5573788235696246E-4 88-89 0.0025920092059052236 90-91 0.0071493880294748475 92-93 0.002734427294141774 94-95 3.4180341176772176E-4 96-97 5.696723529462031E-5 98-99 5.69672352946203E-4 100-101 0.0017090170588386089 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1755395.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.38981291974609 #Duplication Level Percentage of deduplicated Percentage of total 1 76.29152960317144 40.73190492874594 2 13.670572923723848 14.597386618067251 3 4.3527553983803875 6.971783892148313 4 1.8983544293330632 4.054111513498544 5 1.008315641762386 2.6916891738873754 6 0.5878294583270935 1.8830462885279526 7 0.39210751389980836 1.4654182768076254 8 0.26927359110742516 1.1501173322762908 9 0.21206054782383085 1.0189685678375877 >10 1.1384754521977583 11.396778105039402 >50 0.11098535009069221 4.093050769711392 >100 0.06205983603433454 6.145914614172712 >500 0.00428698426264773 1.5256654439705946 >1k 0.0010717460656619324 0.9380127376142954 >5k 3.2152381969857973E-4 1.336151737694834 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9330 0.5315043052988074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8799 0.501254703357364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5222 0.2974829027085072 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2436 0.13877218517769505 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2368 0.13489841317766085 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2319 0.13210701864822447 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2255 0.12846111558936876 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.848361764731015E-5 0.0 0.0 0.0 0.0 14-15 5.69672352946203E-5 0.0 0.0 0.0 0.0 16-17 5.69672352946203E-5 0.0 0.0 5.69672352946203E-5 0.0 18-19 5.69672352946203E-5 0.0 0.0 5.69672352946203E-5 0.0 20-21 5.69672352946203E-5 0.0 0.0 5.69672352946203E-5 0.0 22-23 5.69672352946203E-5 0.0 0.0 1.9938532353117104E-4 0.0 24-25 5.69672352946203E-5 0.0 0.0 3.418034117677218E-4 0.0 26-27 5.69672352946203E-5 0.0 0.0 4.8422150000427253E-4 0.0 28-29 1.4241808823655075E-4 0.0 0.0 0.0011963119411870264 0.0 30-31 1.709017058838609E-4 0.0 0.0 0.0025920092059052236 0.0 32-33 1.709017058838609E-4 0.0 0.0 0.0046428296765115545 0.0 34-35 1.709017058838609E-4 0.0 0.0 0.007519675058889879 0.0 36-37 1.709017058838609E-4 0.0 0.0 0.009940782558911242 0.0 38-39 1.709017058838609E-4 0.0 0.0 0.013615169235414252 0.0 40-41 1.709017058838609E-4 0.0 0.0 0.019140991058992418 0.0 42-43 1.709017058838609E-4 0.0 0.0 0.026176444617878027 0.0 44-45 1.709017058838609E-4 0.0 0.0 0.03241435688263895 0.0 46-47 1.709017058838609E-4 0.0 0.0 0.04044673705918041 0.0 48-49 1.709017058838609E-4 0.0 0.0 0.047453707000418704 0.0 50-51 1.709017058838609E-4 0.0 0.0 0.05765084211815574 0.0 52-53 1.709017058838609E-4 0.0 0.0 0.08066560517718235 0.0 54-55 1.709017058838609E-4 0.0 0.0 0.11251028970687509 0.0 56-57 1.709017058838609E-4 0.0 0.0 0.13939882476593587 0.0 58-59 1.709017058838609E-4 0.0 0.0 0.18628285941340839 0.0 60-61 1.709017058838609E-4 0.0 0.0 0.24416157047274262 0.0 62-63 1.709017058838609E-4 0.0 0.0 0.2839816679436822 0.0 64-65 1.709017058838609E-4 0.0 0.0 0.315285163738076 0.0 66-67 2.278689411784812E-4 0.0 0.0 0.39942577026823023 0.0 68-69 2.278689411784812E-4 0.0 0.0 0.49601371771025893 0.0 70-71 2.848361764731015E-4 0.0 0.0 0.5366028728576759 0.0 72-73 2.848361764731015E-4 0.0 0.0 0.6129389681524671 0.0 74-75 2.848361764731015E-4 0.0 0.0 0.7267879878887658 0.0 76-77 2.848361764731015E-4 0.0 0.0 0.8462197966839372 0.0 78-79 2.848361764731015E-4 0.0 0.0 0.9482765987142495 0.0 80-81 3.418034117677218E-4 0.0 0.0 1.0874760381566542 0.0 82-83 3.418034117677218E-4 0.0 0.0 1.2515131921875133 0.0 84-85 3.418034117677218E-4 0.0 0.0 1.4281401052184837 0.0 86-87 3.418034117677218E-4 0.0 0.0 1.6803910231030623 0.0 88-89 3.418034117677218E-4 0.0 0.0 1.98453909234104 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1500 0.0 28.185328 2 GGTATCA 11075 0.0 26.683651 1 GTATCAA 14845 0.0 24.25982 1 GTACATG 9770 0.0 23.925947 1 TACACCG 120 1.7124797E-4 23.747622 5 GTACCTG 1850 0.0 23.627333 1 TACATGG 10050 0.0 22.688171 2 GAGTACT 9500 0.0 22.124676 12-13 ACATGGG 10070 0.0 21.83868 3 GTGTACG 155 3.9006518E-5 21.4568 1 GTACTTT 9915 0.0 21.079464 14-15 CCTACAC 805 0.0 20.651281 3 AGTACTT 9975 0.0 20.071133 12-13 ATCAACG 17900 0.0 20.007517 3 CATGGGA 5920 0.0 19.977453 4 TATCAAC 17960 0.0 19.916496 2 TCAACGC 18020 0.0 19.873714 4 TACCGTG 120 0.004768815 19.788557 7 CAACGCA 18170 0.0 19.761368 5 GTAAGGT 685 0.0 19.414667 4 >>END_MODULE