FastQCFastQC Report
Thu 26 May 2016
SRR936474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769713
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78000.4407494322525743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64680.365482990744827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37740.21325491760528403No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22540.12736528465350033No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20810.11758968827148808No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18910.10685348415251514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG94300.025.7514971
TACCTGG14400.024.7488612
GTATCAA133850.024.1070841
TACATGG99400.023.9979292
ACATGGG97350.023.9175423
GTACCTG15850.023.6859651
GAGTACT68850.023.42975212-13
GGTATCA99200.023.4255851
GTGTAGC8150.022.1574251
GTACTTT73450.021.76617814-15
GTACACG2901.05501385E-1021.3028951
CATGGGA59000.020.4579774
ATCAACG156250.020.4364593
TCAACGC157350.020.3249424
TATCAAC158150.020.2810762
CATGGGG36750.020.1719674
CAACGCA159350.019.9218625
AGTACTT73100.019.85755212-13
AACGCAG161650.019.7260486
AGTACAT73750.019.0715562
Produced by FastQC (version 0.11.5)