##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936474_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1769713 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30617506906487 31.0 31.0 34.0 28.0 34.0 2 31.343111001614385 31.0 31.0 34.0 28.0 34.0 3 31.377737520151573 31.0 31.0 34.0 28.0 34.0 4 35.023025767454946 37.0 35.0 37.0 32.0 37.0 5 34.94742989399976 37.0 35.0 37.0 32.0 37.0 6 35.07394362814762 37.0 35.0 37.0 32.0 37.0 7 34.90659445910156 37.0 35.0 37.0 32.0 37.0 8 34.89485470242915 37.0 35.0 37.0 32.0 37.0 9 36.38316099842178 39.0 37.0 39.0 32.0 39.0 10-11 36.2566387883233 39.0 35.0 39.0 32.0 39.0 12-13 36.15945410357499 39.0 35.0 39.0 31.5 39.0 14-15 37.21604237523259 39.5 36.5 41.0 31.0 41.0 16-17 37.15539158044271 39.0 36.0 41.0 31.0 41.0 18-19 37.13477157030547 39.0 36.0 41.0 31.0 41.0 20-21 37.0620835129764 39.0 36.0 41.0 31.0 41.0 22-23 36.95078100234332 39.0 36.0 41.0 31.0 41.0 24-25 36.80261064929737 39.0 36.0 41.0 30.0 41.0 26-27 36.595845484550324 39.0 36.0 40.5 30.0 41.0 28-29 36.70089500387916 39.0 36.0 41.0 30.0 41.0 30-31 36.62744128567739 39.0 36.0 41.0 30.0 41.0 32-33 36.480410100394806 39.0 35.0 41.0 30.0 41.0 34-35 36.313540670153856 39.0 35.0 40.5 30.0 41.0 36-37 36.06705663573698 38.5 35.0 40.0 29.5 41.0 38-39 35.87437963104752 38.0 35.0 40.0 28.5 41.0 40-41 35.72245951744718 38.0 35.0 40.0 28.0 41.0 42-43 35.455004286005696 38.0 35.0 40.0 27.0 41.0 44-45 35.17938869183874 38.0 34.0 40.0 26.0 41.0 46-47 34.81642673134005 38.0 34.0 40.0 24.5 41.0 48-49 34.52190129133933 38.0 33.5 40.0 24.0 41.0 50-51 33.80554813125066 37.5 33.0 39.5 23.5 40.5 52-53 33.97525841760783 37.5 33.0 39.5 23.0 40.5 54-55 34.31979196626797 38.0 34.0 40.0 23.0 41.0 56-57 34.25403328110264 38.0 34.0 40.0 23.0 41.0 58-59 34.023578681967074 38.0 33.0 40.0 22.5 41.0 60-61 33.710115425495545 37.0 33.0 40.0 22.0 41.0 62-63 33.381260125229346 37.0 33.0 40.0 21.5 41.0 64-65 33.060630452508406 36.0 32.0 39.0 20.0 41.0 66-67 32.69472366423256 36.0 32.0 39.0 19.5 41.0 68-69 32.32599184161499 35.0 31.5 39.0 18.0 40.5 70-71 31.936115064985113 35.0 31.0 38.5 17.0 40.0 72-73 31.518719984539864 35.0 31.0 37.5 13.5 40.0 74-75 31.09266813319448 35.0 31.0 37.0 10.0 39.0 76-77 30.67214655709711 35.0 30.0 36.5 7.5 39.0 78-79 30.231638971968902 34.0 30.0 36.0 7.0 38.5 80-81 29.83018376426008 34.0 29.5 35.5 2.0 37.0 82-83 29.431523925065818 34.0 29.0 35.0 2.0 37.0 84-85 29.050824342704157 34.0 29.0 35.0 2.0 36.5 86-87 28.704574414043407 34.0 29.0 35.0 2.0 36.0 88-89 28.333934654941224 34.0 28.5 35.0 2.0 36.0 90-91 27.981968545182184 34.0 27.0 35.0 2.0 35.0 92-93 27.6198168855628 34.0 27.0 35.0 2.0 35.0 94-95 27.20619981884068 33.0 26.0 35.0 2.0 35.0 96-97 26.630757077560034 33.0 25.0 35.0 2.0 35.0 98-99 25.96959704765688 33.0 24.5 35.0 2.0 35.0 100-101 23.873302055192 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32541983442857614 1101 2 0.38901272528959296 1101 3 0.45398493568873377 1101 4 0.38196115048330626 1101 5 0.4919754053672136 1101 6 0.5085695858732038 1101 7 0.471747701988356 1101 8 0.48850990209039935 1101 9 0.6739140232675851 1101 10-11 0.5900201431925751 1101 12-13 0.7004549943606193 1101 14-15 0.7732091410306836 1101 16-17 0.8081415610658524 1101 18-19 0.9269622584271247 1101 20-21 0.8628826402405778 1101 22-23 0.6607055683138086 1101 24-25 0.8090048641635335 1101 26-27 0.63435812461924 1101 28-29 0.8234983653986987 1101 30-31 0.6501235101413556 1101 32-33 0.5693380313481811 1101 34-35 0.48420207039117 1101 36-37 0.6782740688957389 1101 38-39 0.5608670692995688 1101 40-41 0.47242639395773267 1101 42-43 0.42433248627327913 1101 44-45 0.5258865777707413 1101 46-47 0.5250161518516379 1101 48-49 0.6381963551802556 1101 50-51 0.6812791141171815 1101 52-53 0.6125149253109043 1101 54-55 0.6598200438599591 1101 56-57 0.6837265649542061 1101 58-59 0.6338694310774073 1101 60-61 0.7080465253673296 1101 62-63 0.8250842923752089 1101 64-65 0.7871721847070461 1101 66-67 0.5843406990512534 1101 68-69 0.4564633817094901 1101 70-71 0.5707695143276119 1101 72-73 0.5535427637304373 1101 74-75 0.2674950068674562 1101 76-77 0.1919937720319176 1101 78-79 0.32443267546630494 1101 80-81 0.22921058571499842 1101 82-83 0.2164689247916698 1101 84-85 0.11989359252347143 1101 86-87 0.3427968097708991 1101 88-89 0.33971316128031503 1101 90-91 0.3364970913072334 1101 92-93 0.2723106173656795 1101 94-95 0.09303910797441262 1101 96-97 0.07152834304396194 1101 98-99 0.15480696223835722 1101 100-101 0.5806430708254915 1102 1 0.19454037377142086 1102 2 0.2560569814247735 1102 3 0.2778216724346976 1102 4 0.2853418590650776 1102 5 0.31931770300890605 1102 6 0.3027775397915704 1102 7 0.19546856429528958 1102 8 0.1913386509217787 1102 9 0.47735183113388047 1102 10-11 0.48573434511336444 1102 12-13 0.49178434231551904 1102 14-15 0.3950065834136751 1102 16-17 0.44421951056709474 1102 18-19 0.5271738968653636 1102 20-21 0.2796688123979152 1102 22-23 0.034607723841723725 1102 24-25 0.34015898794089594 1102 26-27 0.36488451538184563 1102 28-29 0.20183738671457263 1102 30-31 0.15745424428013877 1102 32-33 0.16648432402450908 1102 34-35 0.09916256175625193 1102 36-37 0.17669575848391617 1102 38-39 0.15320211880356993 1102 40-41 -0.15774231826785723 1102 42-43 0.261381363021421 1102 44-45 0.15456406497128228 1102 46-47 0.48032518826230586 1102 48-49 0.6222127451887118 1102 50-51 0.45862093293887796 1102 52-53 0.343375891545719 1102 54-55 0.27561428054307413 1102 56-57 0.49651363250493574 1102 58-59 0.567885043003379 1102 60-61 0.24734418022669047 1102 62-63 0.27988847295259234 1102 64-65 0.43141401514012756 1102 66-67 0.3287124692607506 1102 68-69 0.4261801117998587 1102 70-71 0.5328828821439693 1102 72-73 0.48831102083762445 1102 74-75 0.38949235659877957 1102 76-77 0.4492891931960017 1102 78-79 0.46269045299935385 1102 80-81 0.4763463443514162 1102 82-83 0.557350755607807 1102 84-85 0.42576590080693677 1102 86-87 0.340380863628571 1102 88-89 0.06532266920897456 1102 90-91 0.0074489974297868855 1102 92-93 -0.07079533233188684 1102 94-95 0.0872833923599714 1102 96-97 0.016020536482454162 1102 98-99 0.06281995501894855 1102 100-101 0.37020407668265776 1103 1 0.2794217719580345 1103 2 0.3936437801416197 1103 3 0.3485695930295165 1103 4 0.3407993748900324 1103 5 0.5168455845162185 1103 6 0.4242525592098545 1103 7 0.5269881626125041 1103 8 0.2307552911819144 1103 9 0.6434075001937671 1103 10-11 0.6253032387408339 1103 12-13 0.7319011496516907 1103 14-15 0.6891225655821103 1103 16-17 0.7654604017715911 1103 18-19 0.7263808546516657 1103 20-21 0.667172598307161 1103 22-23 0.6232814505361333 1103 24-25 0.7011122434143431 1103 26-27 0.6841367465151791 1103 28-29 0.7704377703533751 1103 30-31 0.6277169849224506 1103 32-33 0.55659987668556 1103 34-35 0.5434557157235602 1103 36-37 0.6433262146429399 1103 38-39 0.521791239544747 1103 40-41 0.5778830439101696 1103 42-43 0.6728552794927438 1103 44-45 0.5829852868381096 1103 46-47 0.6228615344499318 1103 48-49 0.5545414047113226 1103 50-51 0.10723209511234444 1103 52-53 0.36375327448015327 1103 54-55 0.595806059094663 1103 56-57 0.5658943948922186 1103 58-59 0.5121481431246977 1103 60-61 0.6182133082741643 1103 62-63 0.5157319443527086 1103 64-65 0.30930115390310675 1103 66-67 0.3460573315995461 1103 68-69 0.22678845416393045 1103 70-71 0.3515325774329874 1103 72-73 0.2034433073634041 1103 74-75 0.1889409998281124 1103 76-77 0.27715520673131877 1103 78-79 0.4301802157256951 1103 80-81 0.5266045448204864 1103 82-83 0.5616499145086173 1103 84-85 0.4239619405853894 1103 86-87 0.6118641113813901 1103 88-89 0.501535569486812 1103 90-91 0.6546215140921632 1103 92-93 0.5641338894018872 1103 94-95 0.3990990473208136 1103 96-97 0.48444503217600143 1103 98-99 0.39083900974087626 1103 100-101 0.312998769231811 1104 1 0.26848714251907424 1104 2 0.29289865399676174 1104 3 0.4371999000975322 1104 4 0.2608509903113969 1104 5 0.2963049012007204 1104 6 0.29263659947930165 1104 7 0.3953410788501657 1104 8 0.43812546190984136 1104 9 0.5455055964124469 1104 10-11 0.45308892370507436 1104 12-13 0.5510734691154866 1104 14-15 0.5437468396351051 1104 16-17 0.2368990554637449 1104 18-19 0.5168749023908816 1104 20-21 0.5394458807653137 1104 22-23 0.46294347719848616 1104 24-25 0.5146881254862308 1104 26-27 0.5359850934084989 1104 28-29 0.3471143462388113 1104 30-31 0.36908847800113875 1104 32-33 0.15921875366319682 1104 34-35 0.36375565044721725 1104 36-37 0.38336778625512835 1104 38-39 0.37993631454525456 1104 40-41 0.3136437993661261 1104 42-43 0.38884596583652353 1104 44-45 0.06878726060643459 1104 46-47 0.13100551590337517 1104 48-49 0.022319619907925414 1104 50-51 -0.11516257106979566 1104 52-53 0.11550463216089213 1104 54-55 0.3165280188655899 1104 56-57 0.10282474099145844 1104 58-59 0.1965391047058631 1104 60-61 0.05886314441034557 1104 62-63 0.10662000551059236 1104 64-65 0.11272008673576295 1104 66-67 0.02328177456349323 1104 68-69 -0.037562668656548226 1104 70-71 0.06689405362057599 1104 72-73 -0.050543092275891155 1104 74-75 -0.11178191889947087 1104 76-77 0.015212831614395128 1104 78-79 0.17538417066483092 1104 80-81 0.30834895427142683 1104 82-83 0.04837930030529236 1104 84-85 -0.044556101258766745 1104 86-87 0.13182260706440374 1104 88-89 0.10708515259302231 1104 90-91 -0.04717955792043327 1104 92-93 -0.2846680877312018 1104 94-95 -0.2725028813597312 1104 96-97 0.13026532706358296 1104 98-99 0.1834734655582082 1104 100-101 0.1704826688542873 1105 1 0.11697918696618714 1105 2 0.19763782384631412 1105 3 0.23580059600010728 1105 4 0.2993572660994559 1105 5 0.4986390744164666 1105 6 0.3362838222014588 1105 7 0.49490630638497635 1105 8 0.1284954767744253 1105 9 0.3618138132620885 1105 10-11 0.343248468035533 1105 12-13 0.1371043414600237 1105 14-15 0.1955160086181138 1105 16-17 0.29869748701666055 1105 18-19 0.24730347572395317 1105 20-21 -0.05231335627483702 1105 22-23 -0.1264294272615487 1105 24-25 -0.1498367576967894 1105 26-27 -0.12717164798104363 1105 28-29 -0.04185578127666645 1105 30-31 0.11158661159576866 1105 32-33 0.12609880712238208 1105 34-35 -0.08306012867704027 1105 36-37 0.1169663324586665 1105 38-39 -0.035422201929101504 1105 40-41 -0.29910333270204603 1105 42-43 -0.09327320414634954 1105 44-45 0.1047318117026208 1105 46-47 0.07500960533085532 1105 48-49 0.28976467759984104 1105 50-51 0.1633709342394596 1105 52-53 0.38917965099158636 1105 54-55 0.3910596677680829 1105 56-57 0.4068759238258579 1105 58-59 0.2857270350622869 1105 60-61 0.3504231593415881 1105 62-63 0.09687386996957059 1105 64-65 0.14270755515685352 1105 66-67 -0.16504269334433275 1105 68-69 0.034923433289179684 1105 70-71 0.318878775759476 1105 72-73 0.22257450459387584 1105 74-75 0.06281947023938272 1105 76-77 0.09049312570655488 1105 78-79 0.001527260632233407 1105 80-81 0.24999837335275643 1105 82-83 0.17982089848154814 1105 84-85 0.0955405352121268 1105 86-87 0.21587125692009224 1105 88-89 0.11672588622364799 1105 90-91 -0.07046421473514641 1105 92-93 0.11400526466612959 1105 94-95 0.0947132083877058 1105 96-97 0.19153841549787032 1105 98-99 -0.013372725974978295 1105 100-101 0.1871153402802861 1106 1 0.05855782042257829 1106 2 0.11728476911836339 1106 3 0.12314686089172255 1106 4 0.265739125601101 1106 5 0.17472072006830786 1106 6 0.26244264095710435 1106 7 0.37645194746649224 1106 8 0.32864018507406456 1106 9 0.3435847579027822 1106 10-11 0.3486605025699703 1106 12-13 0.42550337319947573 1106 14-15 0.45669004549078807 1106 16-17 0.4179056979555469 1106 18-19 0.4927506816385545 1106 20-21 0.4364854391263435 1106 22-23 0.4157128142923554 1106 24-25 0.27379692916618126 1106 26-27 0.33477928808331114 1106 28-29 0.13388658207804838 1106 30-31 0.13778104870701924 1106 32-33 0.06396544920539782 1106 34-35 0.08144527204343177 1106 36-37 0.12109722362992414 1106 38-39 0.05516603172171841 1106 40-41 0.1390504911030206 1106 42-43 0.11740252494742975 1106 44-45 0.15404884719386303 1106 46-47 0.05217237283518017 1106 48-49 -0.243225881440722 1106 50-51 -0.0018621983739635084 1106 52-53 0.013418430860369313 1106 54-55 0.058810668923236165 1106 56-57 0.08269650788307814 1106 58-59 0.1099702846854953 1106 60-61 -0.05305500800321994 1106 62-63 0.0814364556107563 1106 64-65 0.021489630882875588 1106 66-67 0.04094929182726048 1106 68-69 0.17947319632235548 1106 70-71 0.16314359194781147 1106 72-73 0.19133958128508866 1106 74-75 -0.02278959665747493 1106 76-77 -0.006808399736822679 1106 78-79 0.019008749563305116 1106 80-81 -0.19741845778690603 1106 82-83 0.030132873372703983 1106 84-85 0.20257411284547544 1106 86-87 0.1794455519210878 1106 88-89 0.2950806813622293 1106 90-91 0.2841012902144051 1106 92-93 -0.04388783277591557 1106 94-95 -0.016406842232264296 1106 96-97 0.1996846826243619 1106 98-99 0.29230106855320415 1106 100-101 0.47960028628704876 1107 1 0.553308707259049 1107 2 0.4901827269976238 1107 3 0.5873841815270495 1107 4 0.5668496685687288 1107 5 0.5356839363285744 1107 6 0.45084665489082454 1107 7 0.5873991846912503 1107 8 0.6626259956905329 1107 9 0.7971209184601307 1107 10-11 0.8080162278583956 1107 12-13 0.7991779122042999 1107 14-15 1.033610725558546 1107 16-17 1.0622339393028994 1107 18-19 1.1715353161980175 1107 20-21 1.04560787049639 1107 22-23 1.148429090537924 1107 24-25 1.110095705172057 1107 26-27 1.102446508689873 1107 28-29 0.989364994401079 1107 30-31 0.8981178867754807 1107 32-33 1.065276529068619 1107 34-35 1.0074799145924018 1107 36-37 1.1070708262632607 1107 38-39 1.2867102457129604 1107 40-41 1.0750304853505668 1107 42-43 1.1949615674836167 1107 44-45 1.084037502078651 1107 46-47 1.3756543644621715 1107 48-49 1.3880331067667484 1107 50-51 1.3335944824607893 1107 52-53 1.303397977694928 1107 54-55 1.2298007299631522 1107 56-57 1.2603961659317235 1107 58-59 1.4213745223257135 1107 60-61 1.3580192625538103 1107 62-63 1.6937419108534826 1107 64-65 1.353723340256181 1107 66-67 1.3804598539697324 1107 68-69 1.4767623529488603 1107 70-71 1.4286448542916226 1107 72-73 1.4510684170525643 1107 74-75 1.1792708999709447 1107 76-77 1.2990646947631923 1107 78-79 1.1755665457347284 1107 80-81 1.283776614278544 1107 82-83 1.2116539177624652 1107 84-85 1.2789842627288301 1107 86-87 1.3725697729431054 1107 88-89 1.2681032757184347 1107 90-91 1.357809385193793 1107 92-93 1.3760659878114723 1107 94-95 1.3952618484455677 1107 96-97 1.5420339204243696 1107 98-99 1.633147478063929 1107 100-101 1.8459516333197428 1108 1 0.18901307636124187 1108 2 0.04964808414784727 1108 3 -0.024790816239260494 1108 4 0.037681426862604894 1108 5 0.30633321914151423 1108 6 0.20598345995477274 1108 7 0.2898772085951933 1108 8 0.1837405737975999 1108 9 0.2950377426307469 1108 10-11 0.09939898650278423 1108 12-13 0.3544989109740442 1108 14-15 0.4594189785362204 1108 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100-101 -5.273184149231998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4161.0 3 1720.0 4 1704.0 5 1803.0 6 1996.0 7 2438.0 8 3026.0 9 3874.0 10 4877.0 11 6156.0 12 7631.0 13 9266.0 14 10312.0 15 12191.0 16 14967.0 17 18454.0 18 16950.0 19 11678.0 20 11802.0 21 12619.0 22 13912.0 23 15597.0 24 17956.0 25 20761.0 26 24530.0 27 28880.0 28 34259.0 29 41336.0 30 49901.0 31 61266.0 32 75587.0 33 97011.0 34 127281.0 35 172987.0 36 238433.0 37 308990.0 38 245743.0 39 37643.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.91466801398407 18.530531093983303 13.907096135391836 22.647704756640792 2 15.61316820336393 20.239139328976783 39.9573122904889 24.190380177170383 3 19.431081162792932 25.030699600523743 29.61238540275672 25.925833833926603 4 12.632306647877357 16.425497614311528 36.961919991679345 33.980275746131774 5 13.439965718440074 37.93285133369628 33.743762299442416 14.883420648421234 6 31.655825798251286 35.83310339836346 17.561528336281025 14.949542467104221 7 28.867355146045163 33.176954951156254 20.252394708833815 17.703295193964774 8 27.006322338666195 33.937608589246935 19.66402190267012 19.392047169416745 9 27.22888747351592 16.53149352429189 20.21301426029086 36.02660474190134 10-11 25.195724185847595 26.630559062954823 26.759001955757867 21.414714795439714 12-13 26.481711584490995 24.07503615171623 27.39782829658992 22.045423967202847 14-15 23.25460473162862 25.23818795449285 26.213405668809735 25.293801645068793 16-17 22.059059586359073 28.83676489206811 26.78291651217681 22.321259009395998 18-19 21.95182129195359 27.009412752223184 29.463680851033764 21.57508510478947 20-21 22.842585655775117 26.12200523773418 29.342982852030193 21.69242625446051 22-23 22.486839167992404 26.19351721292714 29.27090544828775 22.0487381707927 24-25 22.397585970146725 26.354515428148424 29.44904515286878 21.798853448836073 26-27 22.145352574290296 26.505392977474518 29.411907637755785 21.9373468104794 28-29 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27.430139896790273 27.725416349404348 22.177151744860353 54-55 22.179370360167546 27.349536987984997 27.86404187239092 22.607050779456532 56-57 22.42788784827036 27.744545040498984 27.36552042918639 22.462046682044264 58-59 22.597960188125153 27.206523465069537 27.575631204182923 22.619885142622394 60-61 22.415450311041596 27.199851500170368 28.08113131103086 22.303566877757177 62-63 22.560668954831538 27.30597405415424 28.014291800918357 22.11906519009587 64-65 22.313279019468514 27.401763905625536 27.844562703694653 22.440394371211298 66-67 22.195453279168838 27.567242724777657 27.631972270551984 22.60533172550152 68-69 22.256818925438957 27.516575807938192 27.849193912512284 22.377411354110567 70-71 22.174387624691054 27.524415471170627 27.683639126578424 22.61755777755989 72-73 22.26199497411051 27.573055943111495 27.456845669479108 22.708103413298886 74-75 22.174344102566856 27.909725932533195 27.40437333707041 22.511556627829542 76-77 22.43466349996652 27.53771828630891 27.553907786528022 22.473710427196554 78-79 22.366716738712945 27.589575028227355 27.44345697784347 22.60025125521623 80-81 22.25135461875967 28.005298089601805 27.330489674829327 22.412857616809198 82-83 22.336064763198536 27.932576383877688 27.308101559237517 22.423257293686255 84-85 22.246377753452713 27.779843587441107 27.218938589506603 22.754840069599577 86-87 22.479415249259073 27.704484891707015 27.28356449530664 22.53253536372727 88-89 22.450304106039003 27.673847332721902 27.10075592799103 22.775092633248057 90-91 22.27681422330728 27.696680964571712 27.389473803186227 22.637031008934777 92-93 22.33681763157389 27.705539875962298 27.392789446735787 22.56485304572802 94-95 22.144341746572092 27.44641137618163 27.448134937318887 22.961111939927395 96-97 22.203028127448412 27.348364061308462 27.412896905955247 23.03571090528788 98-99 21.98510831064557 27.591166947096298 27.204801422476233 23.218923319781904 100-101 22.059363056316666 27.515702560629606 27.491620872831163 22.933313510222565 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 333.0 1 269.0 2 339.0 3 493.5 4 441.0 5 418.0 6 691.5 7 1313.5 8 2097.0 9 2546.5 10 2363.0 11 2060.0 12 2094.5 13 2238.0 14 2344.5 15 2500.5 16 2627.5 17 2674.5 18 2829.0 19 3065.5 20 3345.5 21 3847.5 22 4501.0 23 5330.0 24 6385.5 25 7851.5 26 9819.5 27 12240.0 28 14558.5 29 17263.0 30 20714.0 31 24236.5 32 28593.5 33 31876.5 34 34821.0 35 38207.0 36 42257.0 37 46775.5 38 48741.0 39 49478.5 40 50590.5 41 52890.5 42 55038.0 43 57390.0 44 60438.0 45 63544.0 46 63899.0 47 64606.5 48 74244.5 49 74301.5 50 64714.5 51 60303.5 52 56873.0 53 56254.0 54 55827.0 55 52728.5 56 48679.0 57 44241.0 58 40474.0 59 36288.5 60 30853.0 61 25931.5 62 22204.0 63 17669.5 64 13054.0 65 10230.0 66 7706.0 67 5921.5 68 4419.0 69 3050.5 70 2367.5 71 1880.5 72 1515.0 73 1369.5 74 1111.5 75 873.0 76 671.5 77 399.0 78 250.5 79 163.0 80 97.5 81 68.0 82 50.0 83 30.0 84 19.0 85 15.5 86 13.0 87 8.0 88 6.5 89 5.5 90 4.0 91 2.5 92 2.5 93 3.5 94 3.0 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015313217453903544 2 0.0066112414837886145 3 0.008249925270368698 4 0.03514694190526938 5 0.04701327277360792 6 0.06198745220270179 7 0.02825316873413938 8 0.024354231448828145 9 0.014352609716942804 10-11 0.04709803227981034 12-13 0.04401843688778915 14-15 0.020596560007187608 16-17 0.02537134552325716 18-19 0.03223686552565303 20-21 0.02466501630490368 22-23 0.014578635066815919 24-25 0.0032773675731601675 26-27 0.0028535700421480773 28-29 0.0015821774491118052 30-31 0.006865520002395868 32-33 0.0014409116054411083 34-35 0.0019212154739214778 36-37 0.006074431277839966 38-39 0.0064982288088520565 40-41 0.003588152429235701 42-43 7.063292183534845E-4 44-45 0.001214886255567993 46-47 0.0027123041984773804 48-49 6.780760496193451E-4 50-51 0.002034228148858035 52-53 8.758482307583206E-4 54-55 0.0028535700421480773 56-57 0.0022037471612628714 58-59 0.0022602534987311504 60-61 7.910887245559026E-4 62-63 0.0015256711116435264 64-65 0.0034186334168308644 66-67 0.0023732661736677076 68-69 0.006554735146320335 70-71 0.010707950950238824 72-73 0.004294481647589186 74-75 0.0032491144044260283 76-77 0.002684051029743241 78-79 0.03364952396236 80-81 0.02237650963743839 82-83 0.0037294182729063975 84-85 0.009154026669861159 86-87 0.007826127739356608 88-89 0.005650633746827876 90-91 0.012600913255426163 92-93 0.014183090704537969 94-95 0.006837266833661729 96-97 0.004040203128981931 98-99 0.022828560337184618 100-101 0.041475651701716604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1769713.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.950495290787735 #Duplication Level Percentage of deduplicated Percentage of total 1 78.0335148813527 43.66013806892738 2 12.774012050602057 14.294246021633523 3 4.061625639617159 6.817498986670276 4 1.6945641996269831 3.7924682508466803 5 0.9043481054524963 2.5299362207676355 6 0.5377263894239556 1.8051634691518392 7 0.34692833075883206 1.358756835545402 8 0.2462857671794332 1.102384852540874 9 0.17853327413468104 0.899012260234925 >10 1.0646421887256183 11.266467050548368 >50 0.09844895899861059 3.857848283690499 >100 0.05478804548532124 5.608406777467794 >500 0.003360257004223698 1.2144180179727249 >1k 0.0010182596982496056 0.9803764896450857 >5k 2.036519396499211E-4 0.8128784143570482 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7800 0.4407494322525743 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6468 0.365482990744827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3774 0.21325491760528403 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2254 0.12736528465350033 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2081 0.11758968827148808 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1891 0.10685348415251514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 5.6506337468278756E-5 12-13 0.0 0.0 0.0 0.0 5.6506337468278756E-5 14-15 2.8253168734139378E-5 0.0 0.0 0.0 5.6506337468278756E-5 16-17 5.6506337468278756E-5 0.0 0.0 2.8253168734139378E-5 5.6506337468278756E-5 18-19 8.475950620241814E-5 0.0 0.0 5.6506337468278756E-5 5.6506337468278756E-5 20-21 1.1301267493655751E-4 0.0 0.0 1.1301267493655751E-4 5.6506337468278756E-5 22-23 1.1301267493655751E-4 0.0 0.0 1.412658436706969E-4 5.6506337468278756E-5 24-25 1.1301267493655751E-4 0.0 0.0 2.2602534987311505E-4 5.6506337468278756E-5 26-27 1.1301267493655751E-4 0.0 0.0 3.672911935438119E-4 5.6506337468278756E-5 28-29 1.6951901240483627E-4 0.0 0.0 0.0013844052679728297 5.6506337468278756E-5 30-31 1.6951901240483627E-4 0.0 0.0 0.002825316873413938 5.6506337468278756E-5 32-33 1.6951901240483627E-4 0.0 0.0 0.005057317203410948 5.6506337468278756E-5 34-35 2.2602534987311505E-4 0.0 0.0 0.0077978745706224675 5.6506337468278756E-5 36-37 2.825316873413938E-4 0.0 0.0 0.010284153419226734 5.6506337468278756E-5 38-39 3.3903802480967255E-4 0.0 0.0 0.013222482967577229 5.6506337468278756E-5 40-41 3.3903802480967255E-4 0.0 0.0 0.016641116384408093 5.6506337468278756E-5 42-43 3.3903802480967255E-4 0.0 0.0 0.02257428181857736 5.6506337468278756E-5 44-45 3.3903802480967255E-4 0.0 0.0 0.028761725771353885 5.6506337468278756E-5 46-47 3.3903802480967255E-4 0.0 0.0 0.0363053218233691 5.6506337468278756E-5 48-49 3.3903802480967255E-4 0.0 0.0 0.042973069644625994 5.6506337468278756E-5 50-51 3.3903802480967255E-4 0.0 0.0 0.05204233680828473 5.6506337468278756E-5 52-53 3.3903802480967255E-4 0.0 0.0 0.07255413730926992 5.6506337468278756E-5 54-55 3.3903802480967255E-4 0.0 0.0 0.09956416661910716 5.6506337468278756E-5 56-57 3.3903802480967255E-4 0.0 0.0 0.12530280333590815 5.6506337468278756E-5 58-59 3.3903802480967255E-4 0.0 0.0 0.1678803286182562 5.6506337468278756E-5 60-61 3.3903802480967255E-4 0.0 0.0 0.2196966400766678 5.6506337468278756E-5 62-63 3.3903802480967255E-4 0.0 0.0 0.251763986589916 5.6506337468278756E-5 64-65 3.3903802480967255E-4 0.0 0.0 0.28004540849278947 5.6506337468278756E-5 66-67 3.3903802480967255E-4 0.0 0.0 0.361216762265972 5.6506337468278756E-5 68-69 3.3903802480967255E-4 0.0 0.0 0.4476714585924384 5.6506337468278756E-5 70-71 3.3903802480967255E-4 0.0 0.0 0.48485262864656586 5.6506337468278756E-5 72-73 3.3903802480967255E-4 0.0 0.0 0.5507390181345788 5.6506337468278756E-5 74-75 3.3903802480967255E-4 0.0 0.0 0.6531285016271 5.6506337468278756E-5 76-77 3.3903802480967255E-4 2.8253168734139378E-5 2.8253168734139378E-5 0.7614511505537904 5.6506337468278756E-5 78-79 3.3903802480967255E-4 5.6506337468278756E-5 5.6506337468278756E-5 0.8586137978304957 5.6506337468278756E-5 80-81 3.3903802480967255E-4 5.6506337468278756E-5 5.6506337468278756E-5 0.992251285942975 5.6506337468278756E-5 82-83 3.955443622779513E-4 5.6506337468278756E-5 5.6506337468278756E-5 1.1480392583430195 5.6506337468278756E-5 84-85 3.955443622779513E-4 5.6506337468278756E-5 5.6506337468278756E-5 1.3126139662193812 5.6506337468278756E-5 86-87 3.955443622779513E-4 5.6506337468278756E-5 5.6506337468278756E-5 1.5536417486903242 5.6506337468278756E-5 88-89 3.955443622779513E-4 5.6506337468278756E-5 5.6506337468278756E-5 1.8510063496171414 5.6506337468278756E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9430 0.0 25.751497 1 TACCTGG 1440 0.0 24.748861 2 GTATCAA 13385 0.0 24.107084 1 TACATGG 9940 0.0 23.997929 2 ACATGGG 9735 0.0 23.917542 3 GTACCTG 1585 0.0 23.685965 1 GAGTACT 6885 0.0 23.429752 12-13 GGTATCA 9920 0.0 23.425585 1 GTGTAGC 815 0.0 22.157425 1 GTACTTT 7345 0.0 21.766178 14-15 GTACACG 290 1.05501385E-10 21.302895 1 CATGGGA 5900 0.0 20.457977 4 ATCAACG 15625 0.0 20.436459 3 TCAACGC 15735 0.0 20.324942 4 TATCAAC 15815 0.0 20.281076 2 CATGGGG 3675 0.0 20.171967 4 CAACGCA 15935 0.0 19.921862 5 AGTACTT 7310 0.0 19.857552 12-13 AACGCAG 16165 0.0 19.726048 6 AGTACAT 7375 0.0 19.071556 2 >>END_MODULE