FastQCFastQC Report
Thu 26 May 2016
SRR936474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769713
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95350.5387879277600379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87430.49403490848516113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53320.3012917913808623No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23540.13301591840032817No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22810.12889095576514384No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22770.12866493041527072No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21900.12374887905553048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98150.027.7784441
GTATCAA136200.027.066621
GTACATG95550.025.3694231
TACATGG98750.024.3422012
ACATGGG96600.023.556183
GAGTACT96900.022.3970912-13
ATCAACG167100.022.0044733
TATCAAC168150.021.8388142
TCAACGC169050.021.7769054
CAACGCA171050.021.518635
GTACTTT102250.021.2019914-15
AACGCAG175100.021.075166
AGTACTT101550.020.64666712-13
TACACCG1856.5629174E-620.5378155
CATGGGG39100.020.5314464
CATGGGA57850.019.95324
ACACCGT3102.6011548E-1019.9167126
TACCTGG14150.019.4723342
CCTATAC4400.019.4341813
ACGCAGA191550.019.2156727
Produced by FastQC (version 0.11.5)