##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936474_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1769713 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.501472837686112 31.0 31.0 34.0 30.0 34.0 2 31.7042554357684 31.0 31.0 34.0 30.0 34.0 3 31.975680237417027 31.0 31.0 34.0 30.0 34.0 4 35.78548103562555 37.0 35.0 37.0 35.0 37.0 5 35.6042290473088 37.0 35.0 37.0 33.0 37.0 6 35.715176980674265 37.0 35.0 37.0 33.0 37.0 7 35.650412241985 37.0 35.0 37.0 33.0 37.0 8 35.6314475850039 37.0 35.0 37.0 33.0 37.0 9 37.25985343386188 39.0 37.0 39.0 34.0 39.0 10-11 37.150371839953706 39.0 37.0 39.0 33.0 39.0 12-13 37.01631818266578 39.0 37.0 39.0 33.0 39.0 14-15 38.12251845355716 40.0 37.0 41.0 33.0 41.0 16-17 38.03444626332066 40.0 37.0 41.0 33.0 41.0 18-19 38.127889663465204 40.0 37.5 41.0 33.0 41.0 20-21 38.21383552022277 40.0 38.0 41.0 33.0 41.0 22-23 38.13098847101197 40.0 37.0 41.0 33.0 41.0 24-25 38.022812456031005 40.0 37.0 41.0 33.0 41.0 26-27 37.81430011532943 40.0 37.0 41.0 33.0 41.0 28-29 37.72542327484739 40.0 37.0 41.0 32.5 41.0 30-31 37.6876230778663 40.0 37.0 41.0 33.0 41.0 32-33 37.49026085020566 39.5 36.0 41.0 32.5 41.0 34-35 37.31325559568133 39.0 36.0 41.0 32.0 41.0 36-37 37.06139865616628 39.0 36.0 41.0 31.0 41.0 38-39 36.90107322486753 39.0 35.0 41.0 31.0 41.0 40-41 36.655514764258385 39.0 35.0 41.0 30.5 41.0 42-43 36.40069971797686 39.0 35.0 40.5 30.0 41.0 44-45 36.09433846053004 39.0 35.0 40.0 30.0 41.0 46-47 35.63783845177156 38.5 35.0 40.0 28.5 41.0 48-49 35.14664326927587 38.0 34.5 40.0 26.0 41.0 50-51 34.931357231370285 38.0 34.0 40.0 26.0 41.0 52-53 34.64668988700427 38.0 34.0 40.0 25.0 41.0 54-55 34.370469109963025 38.0 34.0 40.0 24.0 41.0 56-57 34.09906465059589 38.0 33.0 40.0 23.5 41.0 58-59 33.73646235293519 37.0 33.0 40.0 22.5 41.0 60-61 33.37086013381831 37.0 32.5 39.5 22.0 41.0 62-63 33.07275077936366 36.0 32.0 39.0 22.0 40.5 64-65 32.622134492994064 36.0 31.5 39.0 20.0 40.0 66-67 32.222166814619094 35.0 31.0 38.5 19.5 40.0 68-69 31.77516128321372 35.0 31.0 38.0 18.0 40.0 70-71 31.310993364460792 35.0 30.5 37.5 16.5 39.5 72-73 30.888693816454985 34.0 30.0 37.0 13.0 39.0 74-75 30.521385388478244 34.0 30.0 36.0 9.5 39.0 76-77 29.109155552340972 32.5 28.0 35.0 8.0 37.0 78-79 29.90535103714557 34.0 29.0 35.0 7.5 37.0 80-81 29.747325131250093 34.0 29.0 35.0 7.0 37.0 82-83 29.409930310733998 34.0 29.0 35.0 2.0 36.5 84-85 28.958206500150023 34.0 29.0 35.0 2.0 36.0 86-87 28.568100872853396 34.0 29.0 35.0 2.0 36.0 88-89 28.13481592778038 33.0 27.5 35.0 2.0 35.0 90-91 27.690483993732315 33.0 27.0 35.0 2.0 35.0 92-93 27.229791497265374 33.0 26.0 35.0 2.0 35.0 94-95 26.69314148678345 32.5 25.0 34.0 2.0 35.0 96-97 25.996613292663838 32.0 24.5 34.0 2.0 35.0 98-99 25.12838578910818 32.0 21.0 34.0 2.0 35.0 100-101 22.26086998287293 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3684061796768212 1101 2 0.4754283903184735 1101 3 0.2192484873517344 1101 4 0.1528729325222784 1101 5 0.18027371671592363 1101 6 0.16132221751584552 1101 7 0.13414781572122791 1101 8 0.24066876881030197 1101 9 0.24469470489146516 1101 10-11 0.30297776911657337 1101 12-13 0.3340825962193392 1101 14-15 0.3534885821438465 1101 16-17 0.31941935231656515 1101 18-19 0.3288737583676138 1101 20-21 0.1354899555923268 1101 22-23 0.17785953680629518 1101 24-25 0.06220304965135881 1101 26-27 0.09462603194612029 1101 28-29 0.16848451415977905 1101 30-31 0.10616766097423636 1101 32-33 0.20732988662835794 1101 34-35 0.2563892614195069 1101 36-37 0.22822096947194837 1101 38-39 0.1418023866756286 1101 40-41 0.11431099433661274 1101 42-43 0.2421360459426083 1101 44-45 0.2686578049912356 1101 46-47 0.2778895918720039 1101 48-49 0.1671691944070517 1101 50-51 0.13667661705422773 1101 52-53 0.057046248328035176 1101 54-55 0.06048125715451391 1101 56-57 0.04307524841359367 1101 58-59 0.10293365603713767 1101 60-61 0.2069437633243396 1101 62-63 0.09267794371145754 1101 64-65 0.08741582800784187 1101 66-67 -0.06392838108629206 1101 68-69 -0.10539222119997405 1101 70-71 -0.19987823843447572 1101 72-73 0.029116007372145702 1101 74-75 -0.013915605013188781 1101 76-77 0.30644481457029116 1101 78-79 0.1899898400856621 1101 80-81 -0.08971595660526077 1101 82-83 0.04802405496231543 1101 84-85 0.189079902100449 1101 86-87 0.22331313376499295 1101 88-89 0.18225399897386296 1101 90-91 0.08047644758945083 1101 92-93 0.05034221257128735 1101 94-95 -0.051479906351364235 1101 96-97 -0.17193451309413987 1101 98-99 -0.2813165853737125 1101 100-101 -0.1920411046148658 1102 1 0.561150979392405 1102 2 0.6011659227435651 1102 3 0.3423315614239897 1102 4 0.21006379136973408 1102 5 0.2288146923356038 1102 6 0.27196973776693767 1102 7 0.3050350811443181 1102 8 0.11958776084632916 1102 9 0.24145189646853993 1102 10-11 0.26784225707245213 1102 12-13 0.28151235033330124 1102 14-15 0.39836658479411824 1102 16-17 0.5014885609587836 1102 18-19 0.4001248671351618 1102 20-21 0.3688315086689258 1102 22-23 0.14198035745588555 1102 24-25 0.26170250319803046 1102 26-27 0.09373393044107559 1102 28-29 0.17648140661800937 1102 30-31 0.3630465087418031 1102 32-33 0.4507308492959652 1102 34-35 0.5392309264202808 1102 36-37 0.5550599075928417 1102 38-39 0.4958236367070512 1102 40-41 0.6075276113057484 1102 42-43 0.4864674157149409 1102 44-45 0.46452002214329013 1102 46-47 0.5125769283998665 1102 48-49 0.6791564530069607 1102 50-51 0.5148330882466254 1102 52-53 0.40037514858623524 1102 54-55 0.7331937245498636 1102 56-57 0.6902624203470822 1102 58-59 0.817450999495918 1102 60-61 0.7492253645462839 1102 62-63 0.5244266422188559 1102 64-65 0.42362766755046977 1102 66-67 0.7062490935452246 1102 68-69 0.6094576028935315 1102 70-71 0.46943490625175954 1102 72-73 0.4589365576081441 1102 74-75 -0.041190987560437264 1102 76-77 0.3614528527609693 1102 78-79 0.41622331032160886 1102 80-81 0.5826251260306918 1102 82-83 0.7069922936349293 1102 84-85 0.7288761282806497 1102 86-87 0.498599917016346 1102 88-89 0.5662951679171222 1102 90-91 0.6900505132620047 1102 92-93 0.8430430805522349 1102 94-95 0.5440635363883466 1102 96-97 0.6804009912888596 1102 98-99 0.3666702159530999 1102 100-101 0.043766371441140706 1103 1 0.53806291462082 1103 2 0.5098778032331346 1103 3 0.304925610995916 1103 4 0.23268500028131456 1103 5 0.12977939161331875 1103 6 0.14828882441013747 1103 7 0.2124855754571442 1103 8 0.27910803143598173 1103 9 0.1841637221025465 1103 10-11 0.2960795960874165 1103 12-13 0.26469576457859034 1103 14-15 0.23834225576811008 1103 16-17 0.211196128283774 1103 18-19 0.33108811915970904 1103 20-21 0.30096175445103057 1103 22-23 0.19688654568867037 1103 24-25 0.17737068189411787 1103 26-27 0.21180192332852243 1103 28-29 0.35088723041143766 1103 30-31 0.4169924328275414 1103 32-33 0.295740393580445 1103 34-35 0.19855818129031633 1103 36-37 0.33257478716445377 1103 38-39 0.341396763550037 1103 40-41 0.3145762886155623 1103 42-43 0.18735257442551045 1103 44-45 0.2877538043001664 1103 46-47 0.23404255234396487 1103 48-49 0.5323775541915552 1103 50-51 0.1648951508975145 1103 52-53 0.3287267797514346 1103 54-55 0.24134429840325566 1103 56-57 0.12890165806484077 1103 58-59 0.216127959167153 1103 60-61 0.24332766200233635 1103 62-63 0.12834744362363182 1103 64-65 0.1349453040226507 1103 66-67 0.07700384190700049 1103 68-69 0.1334900295344248 1103 70-71 0.24704884680116734 1103 72-73 0.29354747118086877 1103 74-75 0.3743397604418526 1103 76-77 0.3582985119643638 1103 78-79 0.44400911218448513 1103 80-81 0.2319987221805313 1103 82-83 0.43739645658863324 1103 84-85 0.646088140948784 1103 86-87 0.4287930027643476 1103 88-89 0.3278777159113595 1103 90-91 0.15420648747055665 1103 92-93 0.2906777553375264 1103 94-95 0.13661741884921597 1103 96-97 -0.044604129536356396 1103 98-99 0.1622328677177265 1103 100-101 0.49694802330555987 1104 1 0.30427012328305736 1104 2 0.331537101074975 1104 3 0.15982705716984924 1104 4 0.06063892325522602 1104 5 0.02216090158310635 1104 6 0.09709988639574618 1104 7 0.0522161591084469 1104 8 0.12796636830231733 1104 9 0.12313706647229594 1104 10-11 0.047529807730832374 1104 12-13 0.026705014304361896 1104 14-15 0.1568641670864892 1104 16-17 0.06738585701680933 1104 18-19 0.18542218264600763 1104 20-21 0.05693883033983127 1104 22-23 0.08727653821889447 1104 24-25 0.055291293742108394 1104 26-27 0.12801981510438054 1104 28-29 -0.030678678511236512 1104 30-31 -0.044810809455384515 1104 32-33 0.06503266674513952 1104 34-35 0.049465657114517114 1104 36-37 0.09974726140721657 1104 38-39 -0.02181648149198878 1104 40-41 -0.020302310836655124 1104 42-43 0.006040368421430742 1104 44-45 -0.09106351613939978 1104 46-47 0.030868658172408914 1104 48-49 0.06361220247316623 1104 50-51 -0.0720113054043523 1104 52-53 -0.26027995986323305 1104 54-55 -0.28934694110710524 1104 56-57 -0.19461162264364873 1104 58-59 -0.4510720219901856 1104 60-61 -0.4854920137693739 1104 62-63 -0.62255851805849 1104 64-65 -0.4320037501432594 1104 66-67 -0.4553369712882436 1104 68-69 -0.3530757447978381 1104 70-71 -0.4803058965012781 1104 72-73 -0.34563120767968414 1104 74-75 -0.3739219203231592 1104 76-77 -0.185113203457675 1104 78-79 -0.07540387175215457 1104 80-81 -0.037691433877046876 1104 82-83 -0.010858158179971156 1104 84-85 0.056496353655465015 1104 86-87 -0.11316261268394001 1104 88-89 -0.27514871764472204 1104 90-91 -0.232943240993297 1104 92-93 -0.10395898515197644 1104 94-95 0.008823826698527881 1104 96-97 -0.27474867963426775 1104 98-99 0.20557770853092094 1104 100-101 0.1376988863665467 1105 1 0.3267278125398967 1105 2 0.2956804543388536 1105 3 0.07431569542715977 1105 4 0.055623792040258024 1105 5 -0.03412455178676055 1105 6 0.0015457639728850836 1105 7 0.11430496667538392 1105 8 0.06147900986582755 1105 9 0.19572357102832427 1105 10-11 0.12883342522437857 1105 12-13 -0.009172879457175043 1105 14-15 0.07847972424182359 1105 16-17 0.054062305647327946 1105 18-19 0.05014070538615556 1105 20-21 0.07942359913887032 1105 22-23 0.13012262310035538 1105 24-25 -0.0024897835737291985 1105 26-27 0.1278519219427494 1105 28-29 0.22732811720403134 1105 30-31 0.1211026293298545 1105 32-33 0.13602147831151967 1105 34-35 0.15394769629569538 1105 36-37 0.1832176588849066 1105 38-39 0.1010688145665668 1105 40-41 -0.04025035144430689 1105 42-43 0.18813112571540813 1105 44-45 -0.14662490421173402 1105 46-47 -0.07920718121008008 1105 48-49 -0.25031593092148796 1105 50-51 -0.2761314785801048 1105 52-53 -0.16453711386166958 1105 54-55 -0.14755970798641016 1105 56-57 -0.16760040421423383 1105 58-59 -0.09051416675659141 1105 60-61 -0.32234402115501126 1105 62-63 -0.13908870383247773 1105 64-65 -0.028942262084918013 1105 66-67 0.03372747635575024 1105 68-69 -0.0780604969325367 1105 70-71 -0.16173341331559143 1105 72-73 -0.1940561781838177 1105 74-75 -0.3215238393878188 1105 76-77 -0.21240729895097488 1105 78-79 -0.07198268405176478 1105 80-81 -0.24419877284958957 1105 82-83 0.06458502977371694 1105 84-85 6.225093270053605E-4 1105 86-87 -0.05991548394862889 1105 88-89 -0.1706508473465611 1105 90-91 -0.25603209188750853 1105 92-93 -0.16207501557438775 1105 94-95 -0.1391887848691873 1105 96-97 -0.22034129666788616 1105 98-99 -0.23886340304679266 1105 100-101 0.1792209899387167 1106 1 0.3597752212696044 1106 2 0.35134426175948263 1106 3 0.17437114831803768 1106 4 0.028412765156083708 1106 5 -0.11899792504058837 1106 6 -0.040295327876378906 1106 7 0.01013426675374518 1106 8 0.02944372115461391 1106 9 -3.394133987697501E-4 1106 10-11 -0.03299207835630824 1106 12-13 -0.01786557406260414 1106 14-15 0.10812369433357816 1106 16-17 0.024205603180725177 1106 18-19 0.015553885295169323 1106 20-21 -0.00914151191357604 1106 22-23 -0.11626225348553731 1106 24-25 -0.1835258726503497 1106 26-27 -0.15668272773326208 1106 28-29 -0.09672712337351896 1106 30-31 -0.1970240407170678 1106 32-33 -0.10727242340695398 1106 34-35 -0.2982841778024934 1106 36-37 -0.017072037074022717 1106 38-39 -0.041878541503855615 1106 40-41 -0.0439637396159398 1106 42-43 -0.18789311152797694 1106 44-45 -0.13984833745804792 1106 46-47 -0.11583793619350757 1106 48-49 -0.1682808517300174 1106 50-51 -0.12181390532152392 1106 52-53 0.029026439760713174 1106 54-55 -0.0689744411350901 1106 56-57 -0.21030346026913804 1106 58-59 -0.1566764495719184 1106 60-61 -0.017675642013678328 1106 62-63 -0.4498265904487724 1106 64-65 -0.3544708454660892 1106 66-67 -0.2103444419930085 1106 68-69 -0.18221694951193612 1106 70-71 -0.22312828399302376 1106 72-73 -0.16415477817292157 1106 74-75 -0.10797028684097043 1106 76-77 -0.2589157921827905 1106 78-79 -0.14294855607990442 1106 80-81 -0.14118713612329614 1106 82-83 0.13769484744963734 1106 84-85 0.2330920626169508 1106 86-87 0.13621328967646562 1106 88-89 0.030889122734020447 1106 90-91 -0.1165324529598557 1106 92-93 0.12088376630578779 1106 94-95 0.1953552931950746 1106 96-97 0.31384268680519867 1106 98-99 0.4144071302368175 1106 100-101 0.5635423485205742 1107 1 0.5928789891261808 1107 2 0.588898191004315 1107 3 0.45221941139688226 1107 4 0.2193941975168272 1107 5 0.2115412076173655 1107 6 0.271982963005982 1107 7 0.33937110801800685 1107 8 0.3151632571234231 1107 9 0.4045309966232793 1107 10-11 0.47165542412044204 1107 12-13 0.5142914359402724 1107 14-15 0.5306765050459248 1107 16-17 0.504561775882145 1107 18-19 0.48919486832543413 1107 20-21 0.45455651627343485 1107 22-23 0.5029245733975927 1107 24-25 0.5699295937776938 1107 26-27 0.5344944478124205 1107 28-29 0.662924214071758 1107 30-31 0.6722714436074995 1107 32-33 0.6651070147701574 1107 34-35 0.5881024828986625 1107 36-37 0.6581956117707009 1107 38-39 0.8307405822058413 1107 40-41 0.8405397917509063 1107 42-43 0.6148712616144607 1107 44-45 0.6302445007382289 1107 46-47 0.7552719676126998 1107 48-49 0.6790314744979753 1107 50-51 0.7643969859890731 1107 52-53 0.43530300490724017 1107 54-55 0.5012286524061906 1107 56-57 0.6631281973960057 1107 58-59 0.4456045577464778 1107 60-61 0.3238338974705215 1107 62-63 0.24967726049877825 1107 64-65 0.39306678516252447 1107 66-67 0.37888409017278946 1107 68-69 0.700250852731525 1107 70-71 0.40438921305085174 1107 72-73 0.4061817403148247 1107 74-75 0.6101275687724659 1107 76-77 0.8084070802086316 1107 78-79 0.8871958944010672 1107 80-81 0.8588256471051103 1107 82-83 0.7897601377887113 1107 84-85 0.7177705644225831 1107 86-87 1.0277116438358114 1107 88-89 1.1729067955472878 1107 90-91 1.0961916530131042 1107 92-93 1.2623086169018052 1107 94-95 1.2117202224079442 1107 96-97 1.1972532521434722 1107 98-99 1.648450465381103 1107 100-101 1.739764744736739 1108 1 0.38479061738479103 1108 2 0.334032521824458 1108 3 0.09217329424143372 1108 4 0.03000457971876358 1108 5 -0.1587273165649279 1108 6 -0.07909505127589966 1108 7 -0.010657070869818597 1108 8 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-1.3669425662438677 2316 80-81 -1.2638000458111698 2316 82-83 -1.1389123991721988 2316 84-85 -1.3687797611432053 2316 86-87 -1.2798065381583683 2316 88-89 -1.3797375794890137 2316 90-91 -1.374609709910466 2316 92-93 -1.579854733876779 2316 94-95 -1.814385862348864 2316 96-97 -1.8194315911396721 2316 98-99 -2.188045491666152 2316 100-101 -3.0885399577367885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 16.0 4 19.0 5 65.0 6 135.0 7 329.0 8 799.0 9 1503.0 10 2532.0 11 3983.0 12 5682.0 13 7918.0 14 10331.0 15 13308.0 16 16951.0 17 21940.0 18 18832.0 19 12191.0 20 10750.0 21 11097.0 22 11760.0 23 13322.0 24 15539.0 25 18824.0 26 23024.0 27 28639.0 28 34873.0 29 43292.0 30 53666.0 31 66358.0 32 82643.0 33 105099.0 34 137092.0 35 184376.0 36 257415.0 37 334971.0 38 208031.0 39 12406.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.503496371754316 19.371823393978215 14.894446623313826 22.230233610953643 2 15.77442342795463 20.99779034874061 39.30410113534255 23.923685087962205 3 19.56706375375289 25.080849914417474 30.29954730828978 25.052539023539854 4 13.027391345627828 17.13151553238721 36.66291035025296 33.178182771732004 5 13.221070309140522 38.03192947104982 33.959969780410724 14.787030439398933 6 31.57822765612277 36.37160375721939 17.064066320358158 14.986102266299678 7 28.354654116232407 33.545665314093306 20.264811299911344 17.83486926976295 8 27.13157444173151 34.18452596550966 19.33940701119334 19.344492581565486 9 27.014373609880472 16.276547892443176 20.2227920833258 36.48628641435055 10-11 25.261801442553143 26.775128912400593 26.57958834486677 21.383481300179493 12-13 26.605249789654685 24.144845282472993 27.555010456521384 21.69489447135094 14-15 23.130960537375422 25.25020306970765 26.512865790347302 25.105970602569627 16-17 21.780678562004123 28.78862278798879 27.23619592555403 22.19450272445306 18-19 21.61950034256977 26.570389258142214 30.410080733452467 21.40002966583555 20-21 22.397021437939372 25.664472148873855 30.60281525874535 21.335691154441427 22-23 22.111336480213055 25.649047474147302 30.50735736410257 21.732258681537072 24-25 22.047642753373005 25.895300537431776 30.72481244133936 21.332244267855863 26-27 21.874479082201464 26.06377418259345 30.54229697131682 21.519449763888268 28-29 21.5077246988636 26.1311014836869 30.95564082989728 21.40553298755222 30-31 21.572232777922967 25.962267882495038 30.789097099105078 21.676402240476914 32-33 21.319665435817512 26.18886098939025 30.63393205023266 21.85754152455958 34-35 22.172502586595137 25.98210093117425 30.32111074130602 21.524285740924597 36-37 22.228711245977323 25.755359095416903 30.253891295195956 21.762038363409815 38-39 21.888194237254424 25.86858765720072 30.523695380943337 21.719522724601518 40-41 21.964650597180906 25.960049682196683 30.5275116859398 21.547788034682604 42-43 21.82904958445333 26.160777130056374 30.08827452838639 21.921898757103907 44-45 21.998759089275502 26.288179477732548 29.691812249529292 22.02124918346266 46-47 22.409141495805617 26.563855729412268 29.016264726814917 22.010738047967198 48-49 22.54434188204206 26.788396571776037 28.57009651063041 22.09716503555149 50-51 22.511454602423512 26.99455021702788 28.43490449106305 22.059090689485554 52-53 22.65920052710139 27.091107226538607 28.36289190381191 21.886800342548096 54-55 22.198264352658583 26.990861757127483 28.464463817322173 22.34641007289176 56-57 22.49649506766596 27.30860752612524 27.993037037288186 22.201860368920617 58-59 22.72903343377451 26.835537812360055 28.058603401438386 22.376825352427048 60-61 22.394049305255937 26.85625097302595 28.629439788518198 22.120259933199915 62-63 22.58910133124705 26.824895113825765 28.64445727179258 21.94154628313461 64-65 22.369729448682282 26.908644954554894 28.52121501546391 22.20041058129891 66-67 22.22847893276403 27.18048795372885 28.20537669619878 22.38565641730834 68-69 22.256936027335566 27.10601008414378 28.41916382862945 22.2178900598912 70-71 22.27139589018764 27.102379143483486 28.201779801733807 22.42444516459507 72-73 22.35989677422271 27.08035144681652 28.064635339176462 22.495116439784304 74-75 22.31195114688088 27.52217449948099 27.77636825858204 22.389506095056092 76-77 22.434226140023732 27.19073854325592 28.027716562129175 22.347318754591175 78-79 22.394758874252034 27.085205873718277 27.98925259537276 22.53078265665693 80-81 22.358390146629027 27.588302795392167 27.729221055736886 22.32408600224192 82-83 22.4798666526892 27.371971238438565 27.739021070468766 22.409141038403472 84-85 22.286550158695984 27.304326990862513 27.627172233387782 22.781950617053724 86-87 22.490968078451274 27.216698868699908 27.686844786576525 22.605488266272292 88-89 22.567793956562582 27.244585018126244 27.496385490414628 22.69123553489655 90-91 22.301970419127358 27.218918691315697 27.817171465405615 22.661939424151324 92-93 22.323267083918186 27.162994808089163 27.796783139964603 22.71695496802805 94-95 22.0719106863534 26.936048246367505 27.850274414777708 23.141766652501378 96-97 22.25816031201056 26.779408727219916 27.771267982270125 23.191162978499403 98-99 21.99582269274696 26.93362930127868 27.56370082749337 23.506847178480992 100-101 21.82449430195204 27.06388408765254 27.735595655180152 23.376025955215272 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 625.0 1 492.0 2 537.0 3 682.5 4 522.0 5 468.5 6 803.0 7 1553.5 8 2370.0 9 2889.0 10 2757.5 11 2319.0 12 2319.5 13 2514.5 14 2733.0 15 2959.5 16 3068.0 17 3202.0 18 3460.5 19 3699.0 20 4003.0 21 4494.0 22 5101.5 23 5869.5 24 6899.0 25 8373.0 26 10484.5 27 13021.5 28 15202.5 29 17907.5 30 21461.0 31 24817.0 32 29065.0 33 32514.5 34 35092.0 35 38013.5 36 42144.5 37 46823.5 38 48979.5 39 49473.5 40 50407.5 41 52753.0 42 54822.5 43 57189.0 44 60294.0 45 62475.5 46 62243.0 47 64013.5 48 74016.5 49 73786.0 50 64000.5 51 59378.5 52 55915.5 53 55361.5 54 55307.5 55 52148.0 56 47935.0 57 43704.0 58 39946.0 59 35733.0 60 30367.5 61 25572.0 62 21719.0 63 17167.5 64 12815.0 65 9996.0 66 7452.0 67 5840.5 68 4361.5 69 2946.0 70 2277.0 71 1817.5 72 1489.0 73 1297.5 74 1068.0 75 882.5 76 631.5 77 389.5 78 269.0 79 185.5 80 119.5 81 83.0 82 58.5 83 34.0 84 16.5 85 9.5 86 5.5 87 3.0 88 1.0 89 1.5 90 2.0 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0193743279277487 2 0.011470786506060588 3 0.004294481647589186 4 0.014070078029601408 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004577013334930579 10-11 1.9777218113897565E-4 12-13 2.2602534987311502E-4 14-15 2.8253168734139378E-5 16-17 0.0 18-19 2.8253168734139378E-5 20-21 0.0 22-23 0.0013278989305045507 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.8253168734139378E-5 32-33 0.0038141777791088163 34-35 6.780760496193451E-4 36-37 0.0032773675731601675 38-39 5.6506337468278756E-5 40-41 0.003672911935438119 42-43 0.0099733685631512 44-45 0.0025427851860725438 46-47 0.0013844052679728295 48-49 0.009436558357202552 50-51 5.368102059486482E-4 52-53 0.001949468642655617 54-55 0.009690836875809806 56-57 0.0036164055979698404 58-59 0.0011583799180997145 60-61 0.00590491226543513 62-63 0.0031926080669577497 64-65 0.015284964285169404 66-67 0.0032491144044260283 68-69 2.2602534987311502E-4 70-71 0.001214886255567993 72-73 0.0 74-75 0.0 76-77 7.345823870876238E-4 78-79 0.009577824200873249 80-81 0.013985318523398991 82-83 0.011216507987453333 84-85 0.007600102389483493 86-87 0.008814988645051485 88-89 0.02571038354806683 90-91 0.02226349696250183 92-93 0.0020907344863263138 94-95 0.007289317533407959 96-97 0.008899748151253904 98-99 0.008588963295178371 100-101 0.011499039674794726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1769713.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.50114449078842 #Duplication Level Percentage of deduplicated Percentage of total 1 76.31216070496211 40.827879362804445 2 13.609001038302651 14.561942618510395 3 4.393321793999623 7.0514323228591085 4 1.918196535034828 4.105028399305121 5 0.9938913789787794 2.6587163137446317 6 0.6124853065759283 1.966119893136215 7 0.37921651855170846 1.4201962426630093 8 0.26708390339648486 1.1431435605423292 9 0.2000397193487301 0.9632118535895856 >10 1.1412676257388212 11.568548989180654 >50 0.1071972218735521 3.977501346928668 >100 0.06028110142809026 5.9166545074038055 >500 0.004366240439276376 1.5115555542279986 >1k 0.0011714303617570765 0.9828814499052848 >5k 3.1948100775193E-4 1.3451875851987578 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9535 0.5387879277600379 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8743 0.49403490848516113 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5332 0.3012917913808623 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2354 0.13301591840032817 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2281 0.12889095576514384 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2277 0.12866493041527072 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2190 0.12374887905553048 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 5.6506337468278756E-5 0.0 0.0 0.0 0.0 16-17 5.6506337468278756E-5 0.0 0.0 2.8253168734139378E-5 0.0 18-19 5.6506337468278756E-5 0.0 0.0 5.6506337468278756E-5 0.0 20-21 5.6506337468278756E-5 0.0 0.0 1.1301267493655751E-4 0.0 22-23 5.6506337468278756E-5 0.0 0.0 1.412658436706969E-4 0.0 24-25 5.6506337468278756E-5 0.0 0.0 2.2602534987311505E-4 0.0 26-27 5.6506337468278756E-5 0.0 0.0 3.3903802480967255E-4 0.0 28-29 1.9777218113897565E-4 0.0 0.0 0.0013278989305045507 0.0 30-31 2.825316873413938E-4 0.0 0.0 0.0027405573672115197 0.0 32-33 3.107848560755332E-4 0.0 0.0 0.00500081086594267 0.0 34-35 3.672911935438119E-4 0.0 0.0 0.00768486189568591 0.0 36-37 3.955443622779513E-4 0.0 0.0 0.010255900250492594 0.0 38-39 3.955443622779513E-4 0.0 0.0 0.013278989305045508 0.0 40-41 3.955443622779513E-4 0.0 0.0 0.016528103709471536 0.0 42-43 3.955443622779513E-4 0.0 0.0 0.02232000329997011 0.0 44-45 4.5205069974623005E-4 0.0 0.0 0.02853570042148077 0.0 46-47 4.5205069974623005E-4 0.0 0.0 0.03613580281096426 0.0 48-49 4.5205069974623005E-4 0.0 0.0 0.04294481647589185 0.0 50-51 4.5205069974623005E-4 0.0 0.0 0.05221185582068957 0.0 52-53 4.5205069974623005E-4 0.0 0.0 0.0727519094904089 0.0 54-55 4.5205069974623005E-4 0.0 0.0 0.099733685631512 0.0 56-57 4.5205069974623005E-4 0.0 0.0 0.12499201847983261 0.0 58-59 4.5205069974623005E-4 0.0 0.0 0.1673152652435734 0.0 60-61 4.5205069974623005E-4 5.6506337468278756E-5 0.0 0.21952712106426298 0.0 62-63 5.085570372145088E-4 5.6506337468278756E-5 0.0 0.25142494856510633 0.0 64-65 5.085570372145088E-4 5.6506337468278756E-5 0.0 0.2794238387806384 0.0 66-67 5.085570372145088E-4 5.6506337468278756E-5 0.0 0.3601713950228088 0.0 68-69 5.085570372145088E-4 5.6506337468278756E-5 0.0 0.4466260913492753 0.0 70-71 5.085570372145088E-4 5.6506337468278756E-5 0.0 0.48375075506593446 0.0 72-73 5.085570372145088E-4 5.6506337468278756E-5 0.0 0.5499479294100229 0.0 74-75 5.085570372145088E-4 5.6506337468278756E-5 0.0 0.6523374129025441 0.0 76-77 5.085570372145088E-4 5.6506337468278756E-5 0.0 0.7614511505537904 0.0 78-79 5.085570372145088E-4 5.6506337468278756E-5 0.0 0.8587550636741663 0.0 80-81 5.085570372145088E-4 5.6506337468278756E-5 0.0 0.9924490581241139 0.0 82-83 5.085570372145088E-4 5.6506337468278756E-5 0.0 1.148915106573778 0.0 84-85 5.650633746827876E-4 5.6506337468278756E-5 0.0 1.313574573956342 0.0 86-87 6.215697121510663E-4 5.6506337468278756E-5 0.0 1.5546306095960192 0.0 88-89 6.215697121510663E-4 5.6506337468278756E-5 0.0 1.8519387041853679 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9815 0.0 27.778444 1 GTATCAA 13620 0.0 27.06662 1 GTACATG 9555 0.0 25.369423 1 TACATGG 9875 0.0 24.342201 2 ACATGGG 9660 0.0 23.55618 3 GAGTACT 9690 0.0 22.39709 12-13 ATCAACG 16710 0.0 22.004473 3 TATCAAC 16815 0.0 21.838814 2 TCAACGC 16905 0.0 21.776905 4 CAACGCA 17105 0.0 21.51863 5 GTACTTT 10225 0.0 21.20199 14-15 AACGCAG 17510 0.0 21.07516 6 AGTACTT 10155 0.0 20.646667 12-13 TACACCG 185 6.5629174E-6 20.537815 5 CATGGGG 3910 0.0 20.531446 4 CATGGGA 5785 0.0 19.9532 4 ACACCGT 310 2.6011548E-10 19.916712 6 TACCTGG 1415 0.0 19.472334 2 CCTATAC 440 0.0 19.434181 3 ACGCAGA 19155 0.0 19.215672 7 >>END_MODULE