FastQCFastQC Report
Thu 26 May 2016
SRR936473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775105
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74850.4216651972700206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63170.35586627269936144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36490.20556530458761593No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23860.13441458392602126No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22200.12506302444080772No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19720.11109201990868146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG96800.026.1155191
GGTATCA97400.024.9301191
GTATCAA132150.024.7750451
TACATGG100050.024.7475382
TACCTGG15500.024.221822
ACATGGG98650.023.8943923
GTATAGG6400.023.7592321
GTACCTG16650.023.6878831
GAGTACT70100.023.22348612-13
TACACCG1852.5309782E-723.1196985
GTACTTT74350.021.83082214-15
CATGGGA59100.021.7126144
GGACCGT2901.05501385E-1021.3025826
ATCAACG154100.020.724213
TCAACGC155100.020.6223964
TATCAAC156850.020.603252
CAACGCA156800.020.4885865
AGTACTT73600.020.2462512-13
AACGCAG159900.020.0308026
TCGCGCT2550.019.55512660-61
Produced by FastQC (version 0.11.5)