##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936473_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1775105 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.304007931925153 31.0 31.0 34.0 28.0 34.0 2 31.324632627365705 31.0 31.0 34.0 28.0 34.0 3 31.372699643119702 31.0 31.0 34.0 28.0 34.0 4 35.003478667459106 37.0 35.0 37.0 32.0 37.0 5 34.97607916151439 37.0 35.0 37.0 32.0 37.0 6 35.11868086676563 37.0 35.0 37.0 32.0 37.0 7 34.97777708924261 37.0 35.0 37.0 32.0 37.0 8 34.918652136070826 37.0 35.0 37.0 32.0 37.0 9 36.37877195996857 39.0 37.0 39.0 32.0 39.0 10-11 36.292962388140424 39.0 36.0 39.0 32.0 39.0 12-13 36.174808250779535 39.0 35.0 39.0 32.0 39.0 14-15 37.20581345892215 39.5 36.5 41.0 31.0 41.0 16-17 37.085659439864116 39.0 36.0 41.0 31.0 41.0 18-19 37.056684534154314 39.0 36.0 41.0 31.0 41.0 20-21 36.97816946039812 39.0 36.0 41.0 30.5 41.0 22-23 36.845353936809374 39.0 36.0 41.0 30.0 41.0 24-25 36.98314156064008 39.0 36.0 41.0 31.0 41.0 26-27 36.96662760794432 39.0 36.0 41.0 30.5 41.0 28-29 36.86070880314122 39.0 36.0 41.0 30.0 41.0 30-31 36.67623718033581 39.0 36.0 41.0 30.0 41.0 32-33 36.49526309711257 39.0 35.0 41.0 30.0 41.0 34-35 36.271350990504786 39.0 35.0 40.5 30.0 41.0 36-37 36.07475923959427 38.5 35.0 40.0 29.0 41.0 38-39 35.866324245608006 38.0 35.0 40.0 28.5 41.0 40-41 35.68067410096867 38.0 35.0 40.0 27.5 41.0 42-43 35.433747581129 38.0 35.0 40.0 27.0 41.0 44-45 35.15950690240859 38.0 34.0 40.0 26.5 41.0 46-47 34.83525594260621 38.0 34.0 40.0 24.5 41.0 48-49 34.68748412065766 38.0 34.0 40.0 24.0 41.0 50-51 33.75564628571267 37.5 33.0 39.5 23.5 40.5 52-53 33.89191456280051 37.5 33.0 39.5 23.0 40.5 54-55 34.30424059421837 38.0 34.0 40.0 23.0 41.0 56-57 34.228046509924766 38.0 34.0 40.0 23.0 41.0 58-59 33.96856214139446 38.0 33.0 40.0 22.5 41.0 60-61 33.65621808287398 37.0 33.0 40.0 22.0 41.0 62-63 33.31588948259399 37.0 33.0 40.0 20.5 41.0 64-65 32.96396354018495 36.0 32.0 39.0 19.5 41.0 66-67 32.583441824568126 36.0 32.0 39.0 18.5 41.0 68-69 32.18417417561214 35.0 31.0 39.0 17.5 40.0 70-71 31.749575095557727 35.0 31.0 38.0 14.0 40.0 72-73 31.35624230679312 35.0 31.0 37.5 12.0 39.5 74-75 30.935745772785275 35.0 30.5 37.0 8.0 39.0 76-77 30.500628413530464 34.5 30.0 36.0 7.0 39.0 78-79 30.055243210964985 34.0 29.5 36.0 4.0 38.5 80-81 29.592350311671705 34.0 29.0 35.5 2.0 37.0 82-83 29.121554499592982 34.0 29.0 35.0 2.0 37.0 84-85 28.80619061970982 34.0 29.0 35.0 2.0 36.0 86-87 28.470627653012073 34.0 28.5 35.0 2.0 36.0 88-89 28.07186222786821 34.0 27.0 35.0 2.0 36.0 90-91 27.669996141073344 33.5 26.5 35.0 2.0 35.0 92-93 27.295218311029487 33.0 26.0 35.0 2.0 35.0 94-95 26.829064477875956 33.0 25.0 35.0 2.0 35.0 96-97 26.244693694175837 33.0 24.5 35.0 2.0 35.0 98-99 25.52566467899082 33.0 21.5 35.0 2.0 35.0 100-101 23.032988471104524 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5117514494609772 1101 2 0.4987810323699797 1101 3 0.44199946453264616 1101 4 0.5289745610819026 1101 5 0.420230361833859 1101 6 0.31657447625057245 1101 7 0.3776086289027205 1101 8 0.48381483833512107 1101 9 0.6270377362993642 1101 10-11 0.6875410667132797 1101 12-13 0.7354197993426226 1101 14-15 0.8479742392860956 1101 16-17 0.9264821491919335 1101 18-19 0.8349943996501779 1101 20-21 0.9188967523688305 1101 22-23 0.7732432138989509 1101 24-25 0.8051348071522355 1101 26-27 0.5751739431483571 1101 28-29 0.626641081351643 1101 30-31 0.6679847002141486 1101 32-33 0.6284612845527207 1101 34-35 0.617192080795256 1101 36-37 0.7213933525286151 1101 38-39 0.565948040620242 1101 40-41 0.586785294198684 1101 42-43 0.6509549008498894 1101 44-45 0.5801613710866818 1101 46-47 0.6049594784607137 1101 48-49 0.817482494884004 1101 50-51 0.5695888333498402 1101 52-53 0.6655779416748189 1101 54-55 0.8080381711400904 1101 56-57 0.8645328741158806 1101 58-59 0.7888018096056086 1101 60-61 0.7542537371747571 1101 62-63 0.8497302055523548 1101 64-65 0.7222944029923184 1101 66-67 0.5973149359570442 1101 68-69 0.7489592982747268 1101 70-71 0.8074181293072904 1101 72-73 0.553859386307817 1101 74-75 0.5128442247550851 1101 76-77 0.5327937296355323 1101 78-79 0.5938150048174506 1101 80-81 0.6799143308167075 1101 82-83 0.7931973791494151 1101 84-85 0.759641288462177 1101 86-87 0.6647714491213499 1101 88-89 0.6642400141000842 1101 90-91 0.5189350129959713 1101 92-93 0.5146886542602651 1101 94-95 0.33577942086294854 1101 96-97 0.2984073409986827 1101 98-99 0.2796025876662682 1101 100-101 0.6482539284155422 1102 1 0.4238675760284494 1102 2 0.5252562726597318 1102 3 0.41975447063624216 1102 4 0.4340474926827298 1102 5 0.5467934482609422 1102 6 0.5185951421787038 1102 7 0.46462310932777484 1102 8 0.46661244089322196 1102 9 0.696899841558519 1102 10-11 0.6138265148821489 1102 12-13 0.5717881587026525 1102 14-15 0.6378936975711724 1102 16-17 0.614709976964356 1102 18-19 0.7413045781302472 1102 20-21 0.8115005346964637 1102 22-23 0.6440216336455222 1102 24-25 0.45394917682622093 1102 26-27 0.5476663717942145 1102 28-29 0.5583301054404401 1102 30-31 0.3622440151078834 1102 32-33 0.4025921328807911 1102 34-35 0.20449396991137547 1102 36-37 0.41479424421225275 1102 38-39 0.2967198141322456 1102 40-41 0.20367389693024762 1102 42-43 0.5389813523954174 1102 44-45 0.20621940804290517 1102 46-47 0.1947635052246781 1102 48-49 0.4210457190917438 1102 50-51 0.48025460644118567 1102 52-53 0.2745049580040231 1102 54-55 0.4246136227227666 1102 56-57 0.6370004086349113 1102 58-59 0.6235600952398244 1102 60-61 0.4943145888347189 1102 62-63 0.6557860895030174 1102 64-65 0.5008441782284834 1102 66-67 0.5990702498000218 1102 68-69 0.44068883163124895 1102 70-71 0.2950801788106183 1102 72-73 0.23417842896921215 1102 74-75 0.1363170461313885 1102 76-77 0.31800919254488846 1102 78-79 0.2548137478249366 1102 80-81 0.5097941281650584 1102 82-83 0.62199862602046 1102 84-85 0.5263975076790963 1102 86-87 0.5293586614969215 1102 88-89 0.5828511382936945 1102 90-91 0.8365420935760106 1102 92-93 0.5783058732732904 1102 94-95 0.7449464271057877 1102 96-97 0.5261431133908907 1102 98-99 0.44504073453693493 1102 100-101 0.6784602161863802 1103 1 0.24831960817223475 1103 2 0.35648031257928636 1103 3 0.3500393592211779 1103 4 0.31780547524694214 1103 5 0.47723660673936763 1103 6 0.27920498662160753 1103 7 0.27955395787931536 1103 8 0.2041729131816865 1103 9 0.5037497625485159 1103 10-11 0.5050018021211855 1103 12-13 0.5296276969099765 1103 14-15 0.49795701108190116 1103 16-17 0.6080715267653645 1103 18-19 0.48713062618931247 1103 20-21 0.5227666855143411 1103 22-23 0.5925342159632194 1103 24-25 0.6484181716152548 1103 26-27 0.49912777212735904 1103 28-29 0.27600479861204263 1103 30-31 0.32011951112433934 1103 32-33 0.5725115235250371 1103 34-35 0.4594574664876063 1103 36-37 0.5423539473645747 1103 38-39 0.5181309262892313 1103 40-41 0.4002269174834012 1103 42-43 0.5342658814022627 1103 44-45 0.48908560917395505 1103 46-47 0.6304920451460418 1103 48-49 0.7809861651851548 1103 50-51 0.7750907318498079 1103 52-53 0.962204582848841 1103 54-55 1.0379549972773603 1103 56-57 1.1615822220913472 1103 58-59 1.0138519865733784 1103 60-61 0.8288170368812402 1103 62-63 0.8190266294754665 1103 64-65 0.8422059601586724 1103 66-67 0.7842304758538532 1103 68-69 0.9967846798787434 1103 70-71 1.0292742226980245 1103 72-73 1.1034137604738383 1103 74-75 0.8993906252495343 1103 76-77 0.868859226822817 1103 78-79 0.7983869766668192 1103 80-81 0.5033085333631959 1103 82-83 0.4249646871243016 1103 84-85 0.6000496521112026 1103 86-87 0.7574369623335571 1103 88-89 0.9661322286788483 1103 90-91 1.0361618015652816 1103 92-93 1.015175205926898 1103 94-95 0.9054708335728634 1103 96-97 0.9061906387855778 1103 98-99 1.2449063717273638 1103 100-101 1.678778144305749 1104 1 0.3273861296539202 1104 2 0.4202879889545983 1104 3 0.40478419181376424 1104 4 0.4580035261466122 1104 5 0.6232508704423978 1104 6 0.40912390898370177 1104 7 0.41144233210400927 1104 8 0.49353410728807745 1104 9 0.737971111951552 1104 10-11 0.6350801715624641 1104 12-13 0.5572609173483656 1104 14-15 0.6579914003715359 1104 16-17 0.5139420637494752 1104 18-19 0.7533343354571898 1104 20-21 0.7234797104177346 1104 22-23 0.725583989892904 1104 24-25 0.7121272952732696 1104 26-27 0.26108570213569493 1104 28-29 0.5060556854663076 1104 30-31 0.5504484288151943 1104 32-33 0.4235018180854908 1104 34-35 0.3156606389236174 1104 36-37 0.37861101131085917 1104 38-39 0.6734756484707276 1104 40-41 0.6507759682925354 1104 42-43 0.7994464051418504 1104 44-45 0.7816545740419087 1104 46-47 0.91080201347296 1104 48-49 0.6446589124743554 1104 50-51 0.5662318841794658 1104 52-53 0.7121939663975922 1104 54-55 0.7975925316005501 1104 56-57 0.6803016296853528 1104 58-59 0.7153618462519447 1104 60-61 0.68507829933354 1104 62-63 0.8020270869844168 1104 64-65 0.7724370462496779 1104 66-67 0.9731448175176993 1104 68-69 0.9305881016611366 1104 70-71 1.0347659326517693 1104 72-73 0.9125107371488888 1104 74-75 0.6981383647466579 1104 76-77 0.9706794073746003 1104 78-79 0.7558350381829477 1104 80-81 0.8000187154613769 1104 82-83 0.7441520999391997 1104 84-85 0.7897764527614939 1104 86-87 0.8746185469170271 1104 88-89 0.8307516429231647 1104 90-91 0.917469075316685 1104 92-93 0.7793441621414452 1104 94-95 0.574525600840218 1104 96-97 0.7986519969061199 1104 98-99 1.1438625942898462 1104 100-101 1.522723922683923 1105 1 0.23562823508141761 1105 2 0.3700886063709312 1105 3 0.18680763128453037 1105 4 0.25480831034259666 1105 5 0.3397274395993577 1105 6 0.2554460747559588 1105 7 0.34070157219055375 1105 8 0.36600156228217173 1105 9 0.40407163561329185 1105 10-11 0.3912705632364677 1105 12-13 0.34220381414353795 1105 14-15 -0.00417323795839053 1105 16-17 0.26374465321691787 1105 18-19 0.21477251946106435 1105 20-21 0.30488474752926464 1105 22-23 0.21534206030663228 1105 24-25 0.3737570147407894 1105 26-27 0.32590003763253605 1105 28-29 0.04043059045863373 1105 30-31 0.10439717356358358 1105 32-33 0.1468928914318255 1105 34-35 0.011524754742175958 1105 36-37 0.08504408852406442 1105 38-39 -0.03162793762204785 1105 40-41 -0.08848272126306966 1105 42-43 0.232372427753063 1105 44-45 -0.06700447229060558 1105 46-47 0.03595371761270627 1105 48-49 -0.1270708310686146 1105 50-51 0.05370853797028019 1105 52-53 -0.014093967854229561 1105 54-55 -0.055785468690331186 1105 56-57 0.1996803185507403 1105 58-59 0.1305332462521065 1105 60-61 -0.004788726903775853 1105 62-63 -0.008998650747578552 1105 64-65 0.04140098811689796 1105 66-67 0.16342720701915425 1105 68-69 0.21020851469080526 1105 70-71 0.24903092866247434 1105 72-73 0.27123463931214786 1105 74-75 0.18568650539005915 1105 76-77 -0.08410828078167398 1105 78-79 0.13750087905320996 1105 80-81 0.12523418258687258 1105 82-83 0.24385300552150113 1105 84-85 0.31108693002251186 1105 86-87 0.29156890135212876 1105 88-89 0.3415149335205072 1105 90-91 0.27248839730907903 1105 92-93 0.06833428298709521 1105 94-95 0.10959565384730752 1105 96-97 0.3902044694884452 1105 98-99 0.2906288002360746 1105 100-101 0.7197696257899864 1106 1 0.15863917334788624 1106 2 0.22272891743992318 1106 3 0.2904964390160565 1106 4 0.20847413204808163 1106 5 0.38047049178405246 1106 6 0.1501392562531123 1106 7 0.17731330353912256 1106 8 0.18615067610024028 1106 9 0.5998689168415439 1106 10-11 0.2856504156636106 1106 12-13 0.4104658907961536 1106 14-15 0.6141958071841032 1106 16-17 0.5947215978475597 1106 18-19 0.610127030757603 1106 20-21 0.44763952643965865 1106 22-23 0.39976832139193164 1106 24-25 0.4051939367192787 1106 26-27 0.10565923653742715 1106 28-29 0.31550654707540104 1106 30-31 0.3215277983982503 1106 32-33 0.30251856078746897 1106 34-35 0.08176183184423991 1106 36-37 -0.048205519172533684 1106 38-39 0.1290366795643152 1106 40-41 0.016578827353015413 1106 42-43 -0.15034747403625204 1106 44-45 0.0950598526265054 1106 46-47 0.22244101622814583 1106 48-49 0.14645132155345664 1106 50-51 0.09724458493881372 1106 52-53 0.18817792473040384 1106 54-55 0.21655913164816099 1106 56-57 0.13053604078709213 1106 58-59 0.09478676542553188 1106 60-61 0.27271553000550597 1106 62-63 0.2955315225850441 1106 64-65 0.09304763511060798 1106 66-67 0.29751278694759975 1106 68-69 0.19696265666996737 1106 70-71 0.3343591665460188 1106 72-73 0.5387252073366575 1106 74-75 0.3361692119368378 1106 76-77 0.4252299528128525 1106 78-79 0.4116522580863595 1106 80-81 0.6463415183395185 1106 82-83 0.6522941420328117 1106 84-85 0.7283757603548153 1106 86-87 0.7432276192198479 1106 88-89 0.7589424504903555 1106 90-91 0.8722349089549049 1106 92-93 1.0039644211343948 1106 94-95 0.8809167981661652 1106 96-97 1.1045420995392696 1106 98-99 1.3910109589706359 1106 100-101 1.5817340449626442 1107 1 0.18921259757479802 1107 2 0.28349657029854214 1107 3 0.05410086035734096 1107 4 0.1769943793357598 1107 5 0.18752880118385207 1107 6 0.2312006751701503 1107 7 0.18914403606679286 1107 8 0.2585220542580089 1107 9 0.24253771697939186 1107 10-11 0.24457967839741457 1107 12-13 0.38490476350263236 1107 14-15 0.302034086214249 1107 16-17 0.35037991597622664 1107 18-19 0.3822578265979004 1107 20-21 0.5102297554363915 1107 22-23 0.5633967471180554 1107 24-25 0.40259454808007433 1107 26-27 0.24947483411624205 1107 28-29 0.4118501436294295 1107 30-31 0.22972902259353845 1107 32-33 0.2383630871925746 1107 34-35 0.3684392282158626 1107 36-37 0.3535285496528857 1107 38-39 0.2829433294307435 1107 40-41 0.20038312274922276 1107 42-43 0.4275675850168028 1107 44-45 0.4258520370028549 1107 46-47 0.30198281871655297 1107 48-49 0.3970716946161801 1107 50-51 0.4406937684779919 1107 52-53 0.6562151085711179 1107 54-55 0.655322302176792 1107 56-57 0.6104249291091648 1107 58-59 0.7092685512837491 1107 60-61 0.67701710443243 1107 62-63 0.48922919543503696 1107 64-65 0.6938317594550227 1107 66-67 0.5852294286463078 1107 68-69 0.8076595716955808 1107 70-71 0.8118736896239476 1107 72-73 0.651548083317028 1107 74-75 0.7008941590960198 1107 76-77 0.6329760005915013 1107 78-79 0.6386397899764837 1107 80-81 0.7230372283280033 1107 82-83 0.8897659503423547 1107 84-85 0.9948890483547217 1107 86-87 0.8669612958130486 1107 88-89 0.6819708459488538 1107 90-91 0.6261560974865361 1107 92-93 0.7305563199520897 1107 94-95 0.801909780497013 1107 96-97 0.8914351669482308 1107 98-99 1.1591711121452484 1107 100-101 1.6703323305904512 1108 1 -0.10694645404627323 1108 2 0.017025270727909714 1108 3 -0.04191426867532044 1108 4 0.06201247262459475 1108 5 0.19101703625589295 1108 6 0.09577558298762057 1108 7 -0.16368424872603526 1108 8 -0.041581711868929006 1108 9 0.14501185839191066 1108 10-11 -0.1112863683419647 1108 12-13 0.16632891069264844 1108 14-15 0.11627498608508091 1108 16-17 0.2395738236346645 1108 18-19 -0.07106800933721047 1108 20-21 -0.15631287619614653 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86-87 -3.4054669197438443 2316 88-89 -3.8823807715782053 2316 90-91 -3.8841186051625805 2316 92-93 -4.287234497393715 2316 94-95 -4.652338284778359 2316 96-97 -5.832779279811096 2316 98-99 -7.094741874608285 2316 100-101 -6.95210325284981 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4394.0 3 1765.0 4 1750.0 5 1939.0 6 2158.0 7 2475.0 8 3107.0 9 3886.0 10 4971.0 11 6217.0 12 7722.0 13 9506.0 14 10653.0 15 12806.0 16 15621.0 17 19494.0 18 16581.0 19 12062.0 20 12143.0 21 13023.0 22 14413.0 23 16233.0 24 18543.0 25 21472.0 26 24877.0 27 29369.0 28 34940.0 29 41632.0 30 51001.0 31 61774.0 32 76856.0 33 97968.0 34 128759.0 35 173677.0 36 239313.0 37 309801.0 38 237888.0 39 34304.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.891954401285815 18.550908570385182 13.877254519182433 22.67988250914657 2 15.654308827275 20.249391987990776 39.89606882763564 24.200230357098583 3 19.45600670764657 25.036696529142255 29.566780921298413 25.94051584191276 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22.413266662760776 48-49 22.56919181548361 27.208494698981767 27.777284843220052 22.445028642314576 50-51 22.47266841811877 27.38126926477601 27.79828837667318 22.347773940432038 52-53 22.680058376527477 27.417165414527144 27.70952691745513 22.19324929149025 54-55 22.199617739479738 27.34196541626818 27.827270076776056 22.631146767476025 56-57 22.470103897882066 27.71162372170164 27.403781275505047 22.414491104911253 58-59 22.634482164121017 27.197211773226705 27.50492072332309 22.663385339329192 60-61 22.366422041247052 27.264301656919116 28.06192832740949 22.30734797442434 62-63 22.511650022971878 27.35475816431187 27.99323829652622 22.140353516190032 64-65 22.298580815546583 27.415548610109603 27.801497827732913 22.4843727466109 66-67 22.165547715274315 27.595163921472736 27.60257257878459 22.636715784468358 68-69 22.28129422241655 27.52393345708684 27.809295261427643 22.385477059068965 70-71 22.208629050545284 27.50682045135402 27.62417305988617 22.660377438214528 72-73 22.246960033193236 27.480978817341718 27.522076008113526 22.74998514135152 74-75 22.133607431060554 27.94988383334197 27.362853485788456 22.55365524980902 76-77 22.385172965913867 27.59217881501363 27.541107644393666 22.481540574678842 78-79 22.3317965262545 27.623851373562992 27.455682406850023 22.58866969333248 80-81 22.33078383809132 28.009835900247136 27.220507440730646 22.438872820930893 82-83 22.391575384410835 27.89538414040876 27.223774344517064 22.489266130663346 84-85 22.265448480648196 27.77526101973777 27.150729390287193 22.808561109326842 86-87 22.491712067550772 27.686835166129065 27.25841757883923 22.563035187480935 88-89 22.504379640383565 27.626819427546707 27.054078805075793 22.814722126993935 90-91 22.277210012954633 27.668439114487807 27.399938198593215 22.65441267396434 92-93 22.33880980372436 27.664810374501098 27.392184190160496 22.604195631614044 94-95 22.148608626988757 27.408728516547704 27.43064544799701 23.01201740846653 96-97 22.20957688773403 27.3362333717764 27.377953585487564 23.076236155002004 98-99 21.99509041430581 27.547013100586774 27.193233302802206 23.264663182305206 100-101 22.103376110607194 27.495409072298692 27.374891056165566 23.02632376092855 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 318.0 1 261.0 2 326.0 3 462.5 4 435.5 5 459.5 6 710.0 7 1293.5 8 2021.5 9 2451.0 10 2299.5 11 2016.5 12 2075.5 13 2180.5 14 2307.5 15 2450.5 16 2537.5 17 2653.0 18 2752.0 19 3022.0 20 3389.0 21 3813.0 22 4472.0 23 5399.5 24 6398.0 25 7801.0 26 9947.0 27 12363.0 28 14593.0 29 17316.5 30 20736.0 31 24186.5 32 28594.5 33 32245.0 34 35154.0 35 38264.0 36 42531.5 37 46861.5 38 48633.5 39 49537.5 40 50675.0 41 53019.5 42 55162.5 43 57502.0 44 60565.0 45 63781.0 46 64002.5 47 64871.5 48 74712.5 49 74598.0 50 64979.0 51 60404.5 52 56841.5 53 56430.0 54 56252.0 55 53039.0 56 48950.5 57 44511.0 58 40729.5 59 36780.5 60 31326.0 61 26125.5 62 22020.0 63 17357.0 64 12960.5 65 10166.5 66 7735.5 67 6108.5 68 4543.0 69 3082.0 70 2333.5 71 1867.0 72 1527.0 73 1331.5 74 1091.0 75 874.5 76 650.0 77 379.5 78 230.0 79 158.5 80 107.0 81 66.5 82 40.5 83 27.5 84 18.5 85 14.0 86 11.5 87 5.0 88 5.5 89 8.5 90 5.5 91 1.5 92 1.5 93 2.0 94 1.0 95 1.0 96 1.0 97 0.0 98 1.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03768791141932449 2 0.026420972280512982 3 0.02332256401733982 4 0.053405291517966545 5 0.059714777435700986 6 0.050588556733263665 7 0.053461626213660594 8 0.04580010759926877 9 0.045856442294962836 10-11 0.05537700586725856 12-13 0.04957453221077063 14-15 0.04182851155283772 16-17 0.05244760169116757 18-19 0.05653186712898673 20-21 0.013182318792409463 22-23 0.022336706842693815 24-25 0.006478490004816616 26-27 0.01166128200866991 28-29 0.0070136696139101624 30-31 0.009914906442154125 32-33 0.004225102177054315 34-35 0.009351559485213552 36-37 0.010196579920624414 38-39 0.01281614327039809 40-41 0.0020280490449860714 42-43 0.0019153796535979562 44-45 0.0027040653933147617 46-47 5.070122612465178E-4 48-49 0.001211195957422237 50-51 3.6617552201137396E-4 52-53 0.004309604220595401 54-55 0.0077460206579329114 56-57 0.01878762101396819 58-59 0.012027457530681284 60-61 0.012083792226375342 62-63 0.006112314482805243 64-65 5.633469569405754E-5 66-67 0.00870371048473189 68-69 0.01918196388382659 70-71 0.006337653265581473 72-73 0.00250689395838556 74-75 0.0038870940028899697 76-77 0.007379845135921537 78-79 0.005886975700029012 80-81 0.01005574318138927 82-83 0.0065348247005106745 84-85 0.018872123057509276 86-87 0.004760281786147862 88-89 0.007351677788074508 90-91 0.003633587872266711 92-93 0.018618616926886017 94-95 0.012478135096233745 96-97 0.010478253399094702 98-99 0.02329439666949279 100-101 0.03839209511550021 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1775105.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.990763976830415 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8676878662455 43.59871332740454 2 12.944826363998313 14.495814353353628 3 4.003704923565573 6.725114922247015 4 1.7649659640056843 3.952871708711249 5 0.8917306184570378 2.4964339294470506 6 0.5303845873453662 1.7817982948201798 7 0.3494772861158373 1.3697250169522555 8 0.2572765453042894 1.152408825992543 9 0.1858620258607507 0.9365901140002358 >10 1.0468302629991049 11.072554828386368 >50 0.09864659453289548 3.881747083851441 >100 0.05455151504317522 5.684088875136782 >500 0.003041584894878884 1.1880741847527627 >1k 8.110893053010357E-4 0.8805679561319155 >5k 2.0277232632525893E-4 0.7834965788120615 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7485 0.4216651972700206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6317 0.35586627269936144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3649 0.20556530458761593 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2386 0.13441458392602126 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2220 0.12506302444080772 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1972 0.11109201990868146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.633469569405754E-5 0.0 0.0 0.0 0.0 6 5.633469569405754E-5 0.0 0.0 0.0 0.0 7 2.8167347847028767E-4 0.0 0.0 0.0 0.0 8 2.8167347847028767E-4 0.0 0.0 0.0 0.0 9 2.8167347847028767E-4 0.0 0.0 0.0 0.0 10-11 2.8167347847028767E-4 0.0 0.0 0.0 0.0 12-13 2.8167347847028767E-4 0.0 0.0 0.0 0.0 14-15 2.8167347847028767E-4 0.0 0.0 5.633469569405754E-5 0.0 16-17 2.8167347847028767E-4 0.0 0.0 8.45020435410863E-5 0.0 18-19 2.8167347847028767E-4 0.0 0.0 1.1266939138811507E-4 0.0 20-21 2.8167347847028767E-4 0.0 0.0 1.9717143492920138E-4 0.0 22-23 2.8167347847028767E-4 0.0 0.0 2.535061306232589E-4 0.0 24-25 2.8167347847028767E-4 0.0 0.0 2.8167347847028767E-4 0.0 26-27 2.8167347847028767E-4 0.0 0.0 3.380081741643452E-4 0.0 28-29 3.380081741643452E-4 0.0 0.0 0.0010140245224930355 0.0 30-31 3.9434286985840276E-4 0.0 0.0 0.0021407184363741866 0.0 32-33 3.9434286985840276E-4 0.0 0.0 0.0045912776990656885 0.0 34-35 4.2251021770543155E-4 0.0 0.0 0.006703828787592847 0.0 36-37 4.506775655524603E-4 0.0 0.0 0.009943073790001155 0.0 38-39 4.506775655524603E-4 0.0 0.0 0.01414000861920844 0.0 40-41 4.506775655524603E-4 0.0 0.0 0.01966080879722608 0.0 42-43 4.7884491339948904E-4 0.0 0.0 0.026871649846065444 0.0 44-45 5.070122612465178E-4 0.0 0.0 0.032251613284847935 0.0 46-47 5.070122612465178E-4 0.0 0.0 0.03943428698584027 0.0 48-49 5.070122612465178E-4 0.0 0.0 0.04610994842558609 0.0 50-51 5.070122612465178E-4 0.0 0.0 0.056503699781139706 0.0 52-53 5.070122612465178E-4 0.0 0.0 0.078333394362587 0.0 54-55 5.070122612465178E-4 0.0 0.0 0.10669791364454498 0.0 56-57 5.633469569405753E-4 0.0 0.0 0.13362589818630446 0.0 58-59 5.633469569405753E-4 0.0 0.0 0.17610225873962387 0.0 60-61 5.633469569405753E-4 0.0 0.0 0.23359181569540957 0.0 62-63 5.633469569405753E-4 0.0 0.0 0.2705192087228643 0.0 64-65 5.633469569405753E-4 0.0 0.0 0.30133428726751377 0.0 66-67 5.633469569405753E-4 0.0 0.0 0.3847659715904129 0.0 68-69 5.633469569405753E-4 0.0 0.0 0.47738021131144354 0.0 70-71 5.633469569405753E-4 0.0 0.0 0.5154342982527794 0.0 72-73 5.633469569405753E-4 0.0 0.0 0.5887257373507483 0.0 74-75 5.633469569405753E-4 0.0 0.0 0.6935927733852364 0.0 76-77 5.633469569405753E-4 0.0 0.0 0.8053326422943995 0.0 78-79 5.633469569405753E-4 0.0 0.0 0.899862261669028 0.0 80-81 5.633469569405753E-4 0.0 0.0 1.0345585190735196 0.0 82-83 5.633469569405753E-4 0.0 0.0 1.1926900098867392 0.0 84-85 5.633469569405753E-4 0.0 0.0 1.3566239743564465 0.0 86-87 5.633469569405753E-4 2.816734784702877E-5 0.0 1.6007785454944918 0.0 88-89 5.633469569405753E-4 5.633469569405754E-5 0.0 1.905521081851496 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9680 0.0 26.115519 1 GGTATCA 9740 0.0 24.930119 1 GTATCAA 13215 0.0 24.775045 1 TACATGG 10005 0.0 24.747538 2 TACCTGG 1550 0.0 24.22182 2 ACATGGG 9865 0.0 23.894392 3 GTATAGG 640 0.0 23.759232 1 GTACCTG 1665 0.0 23.687883 1 GAGTACT 7010 0.0 23.223486 12-13 TACACCG 185 2.5309782E-7 23.119698 5 GTACTTT 7435 0.0 21.830822 14-15 CATGGGA 5910 0.0 21.712614 4 GGACCGT 290 1.05501385E-10 21.302582 6 ATCAACG 15410 0.0 20.72421 3 TCAACGC 15510 0.0 20.622396 4 TATCAAC 15685 0.0 20.60325 2 CAACGCA 15680 0.0 20.488586 5 AGTACTT 7360 0.0 20.24625 12-13 AACGCAG 15990 0.0 20.030802 6 TCGCGCT 255 0.0 19.555126 60-61 >>END_MODULE