FastQCFastQC Report
Thu 26 May 2016
SRR936473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775105
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95270.5367006458772862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88790.5001957630675369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54550.30730576501108386No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.13441458392602126No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23410.1318795226197887No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22890.1289501184436977No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22140.12472501626664338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108700.028.807261
GTACACG3500.024.4371511
GTATCAA145350.024.3549561
GTACATG97600.023.9530811
TACCTGG15300.023.2839622
TACATGG100050.023.2630162
CATGGGG38250.022.8480424
ACATGGG98200.022.8299673
GGACCGA1754.054802E-621.7121226
GTACCTG16950.021.5857491
GAGTACT100800.020.8993312-13
GTACTTT104950.020.14023814-15
ATCAACG176650.020.0316813
TCAACGC177400.019.9732074
TATCAAC177450.019.9687042
CGCGGAA2150.019.8853882-83
CAACGCA179900.019.6686925
AACGCAG184650.019.214176
AGTACTT104800.019.03650912-13
TATCGCG757.7239925E-419.00051590-91
Produced by FastQC (version 0.11.5)