##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936473_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1775105 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85440466901958 31.0 31.0 34.0 30.0 34.0 2 31.98972905828106 31.0 31.0 34.0 30.0 34.0 3 32.091047571833776 33.0 31.0 34.0 30.0 34.0 4 35.84377825537081 37.0 35.0 37.0 35.0 37.0 5 35.62761864791097 37.0 35.0 37.0 33.0 37.0 6 35.73515087839874 37.0 35.0 37.0 33.0 37.0 7 35.68293030553122 37.0 35.0 37.0 33.0 37.0 8 35.65957281400255 37.0 35.0 37.0 33.0 37.0 9 37.28401643846421 39.0 37.0 39.0 34.0 39.0 10-11 37.15174567138282 39.0 37.0 39.0 33.0 39.0 12-13 37.0504271578301 39.0 37.0 39.0 33.0 39.0 14-15 38.129289534985254 40.0 37.0 41.0 33.0 41.0 16-17 38.053992580720575 40.0 37.0 41.0 33.0 41.0 18-19 38.23494441173902 40.0 37.5 41.0 33.5 41.0 20-21 38.28322014754057 40.0 38.0 41.0 34.0 41.0 22-23 38.193333915458524 40.0 37.5 41.0 33.0 41.0 24-25 38.068348069550815 40.0 37.0 41.0 33.0 41.0 26-27 37.84169978677318 40.0 37.0 41.0 33.0 41.0 28-29 37.74998943724455 40.0 37.0 41.0 32.5 41.0 30-31 37.68463950019858 40.0 37.0 41.0 33.0 41.0 32-33 37.5156773824647 40.0 36.0 41.0 32.5 41.0 34-35 37.33179502057625 39.0 36.0 41.0 32.0 41.0 36-37 37.0582717642055 39.0 36.0 41.0 31.0 41.0 38-39 36.8863962976838 39.0 35.0 41.0 31.0 41.0 40-41 36.647231008869895 39.0 35.0 41.0 30.5 41.0 42-43 36.37967641350794 39.0 35.0 40.0 30.0 41.0 44-45 36.09534421907436 39.0 35.0 40.0 30.0 41.0 46-47 35.67833649277085 38.5 35.0 40.0 28.5 41.0 48-49 35.2692640717028 38.0 35.0 40.0 27.0 41.0 50-51 34.93325662425603 38.0 34.0 40.0 26.0 41.0 52-53 34.63630123288482 38.0 34.0 40.0 24.5 41.0 54-55 34.36405790080023 38.0 34.0 40.0 24.0 41.0 56-57 34.05038603350224 38.0 33.0 40.0 23.5 41.0 58-59 33.7231149706637 37.0 33.0 40.0 23.0 41.0 60-61 33.35930128076931 37.0 32.5 39.5 22.0 41.0 62-63 32.94795350134218 36.0 32.0 39.0 21.5 40.0 64-65 32.52192180180891 35.5 31.0 39.0 20.0 40.0 66-67 32.05952211277643 35.0 31.0 38.5 18.5 40.0 68-69 31.779615008689625 35.0 31.0 38.0 18.0 40.0 70-71 31.34761352145366 35.0 30.5 37.5 16.0 39.5 72-73 30.900532644547788 34.5 30.0 37.0 12.5 39.0 74-75 30.378866038910374 34.0 29.5 36.0 8.0 39.0 76-77 28.965059813363155 32.5 28.0 35.0 7.5 37.0 78-79 29.74035648595435 34.0 29.0 35.0 7.0 37.0 80-81 29.53513820309221 34.0 29.0 35.0 4.5 37.0 82-83 29.208589632725953 34.0 29.0 35.0 2.0 36.5 84-85 28.78724469820095 34.0 29.0 35.0 2.0 36.0 86-87 28.39858430909721 33.0 28.5 35.0 2.0 36.0 88-89 27.97507865731886 33.0 27.0 35.0 2.0 35.0 90-91 27.4855746561471 33.0 26.5 35.0 2.0 35.0 92-93 26.99177654279606 33.0 25.5 34.5 2.0 35.0 94-95 26.39575546235293 32.0 25.0 34.0 2.0 35.0 96-97 25.604174119277452 32.0 23.5 34.0 2.0 35.0 98-99 24.61488362660237 31.5 10.5 34.0 2.0 35.0 100-101 21.325636511642973 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27643103322701634 1101 2 0.26580402449618035 1101 3 0.2936006713542696 1101 4 0.1719814935777535 1101 5 0.10692496737725321 1101 6 0.15986042318414917 1101 7 0.07551083298828587 1101 8 0.13182552237523026 1101 9 0.2888739303316896 1101 10-11 0.22912397163395326 1101 12-13 0.2550422462336641 1101 14-15 0.42205183153416925 1101 16-17 0.33834342603900325 1101 18-19 0.28041473463386524 1101 20-21 0.26244696685650837 1101 22-23 0.19162816238107894 1101 24-25 0.08784339276076025 1101 26-27 0.2345626186551968 1101 28-29 0.26784543519324444 1101 30-31 0.3408572340191256 1101 32-33 0.3635720700680807 1101 34-35 0.21630551479749016 1101 36-37 0.22419442068851225 1101 38-39 0.41692126722249867 1101 40-41 0.37763539221196396 1101 42-43 0.3393033659294318 1101 44-45 0.5148166920329942 1101 46-47 0.5364456418270365 1101 48-49 0.5615026874402531 1101 50-51 0.45800151897331887 1101 52-53 0.5862129445955588 1101 54-55 0.4567167629773081 1101 56-57 0.5294223785042433 1101 58-59 0.5397283142098743 1101 60-61 0.6937081501461577 1101 62-63 0.6098140933159186 1101 64-65 0.5671409443971456 1101 66-67 0.4921338445798682 1101 68-69 0.4747125346155947 1101 70-71 0.6164065006458159 1101 72-73 0.6555951751455744 1101 74-75 0.6294132638864127 1101 76-77 0.35984399329162287 1101 78-79 0.4889340874158137 1101 80-81 0.4089625802361354 1101 82-83 0.577449494894875 1101 84-85 0.47468888588796787 1101 86-87 0.28443424449396915 1101 88-89 0.27660932912477065 1101 90-91 0.3249934944481474 1101 92-93 0.6585189848482393 1101 94-95 0.6324105537560456 1101 96-97 0.51451317761137 1101 98-99 0.6777615546124309 1101 100-101 1.114211588770658 1102 1 0.3273706768168374 1102 2 0.33640827311332444 1102 3 0.3648280044619625 1102 4 0.2712169133275424 1102 5 0.26949385404812887 1102 6 0.26691628461015426 1102 7 0.24238426814669367 1102 8 0.21020457831154715 1102 9 0.26061339208614953 1102 10-11 0.37760149539672483 1102 12-13 0.3223245476796066 1102 14-15 0.5177621434654753 1102 16-17 0.5055465401743362 1102 18-19 0.46582756613747733 1102 20-21 0.3468882253467598 1102 22-23 0.3132510371290991 1102 24-25 0.41826465930724055 1102 26-27 0.16038104598408864 1102 28-29 0.1786957934083233 1102 30-31 0.4600572461882848 1102 32-33 0.5120924491725845 1102 34-35 0.5465076779245166 1102 36-37 0.6212838262922418 1102 38-39 0.6732106908459414 1102 40-41 0.6092107399472297 1102 42-43 0.5806999001170254 1102 44-45 0.5579401356015694 1102 46-47 0.5905810067863442 1102 48-49 0.5507486294196795 1102 50-51 0.5236860328664932 1102 52-53 0.4290046322389216 1102 54-55 0.5416187529570564 1102 56-57 0.5673437978965623 1102 58-59 0.4322355623206633 1102 60-61 0.5706723654970247 1102 62-63 0.6342780578240905 1102 64-65 0.8297077248669211 1102 66-67 0.740787996706672 1102 68-69 0.6928412510001216 1102 70-71 0.5577561133534665 1102 72-73 0.599576369110018 1102 74-75 0.5622058495905158 1102 76-77 0.37044242645352554 1102 78-79 0.3330392254641339 1102 80-81 0.6740417368313096 1102 82-83 0.6168576874905831 1102 84-85 0.6586899776022008 1102 86-87 0.5621413930282451 1102 88-89 0.6766121666456897 1102 90-91 0.6228941385068865 1102 92-93 0.5341615590121123 1102 94-95 0.6531597060832368 1102 96-97 0.8287085049457339 1102 98-99 0.7597935530725657 1102 100-101 1.2333489999628355 1103 1 0.1305901085834904 1103 2 0.12635608014511845 1103 3 0.15925040681568703 1103 4 0.07919749222327965 1103 5 -0.2217919999831537 1103 6 -0.09750403246830075 1103 7 0.04455300995379474 1103 8 0.022423736021387697 1103 9 0.1102736532647981 1103 10-11 0.14563711334209017 1103 12-13 0.1513669620673923 1103 14-15 0.1291933114723065 1103 16-17 0.2669835530605553 1103 18-19 0.09435465930540943 1103 20-21 0.11572268975450584 1103 22-23 0.16548984807238298 1103 24-25 0.048831490435759406 1103 26-27 0.16494241208095417 1103 28-29 0.18920144350468604 1103 30-31 0.02743273984458483 1103 32-33 0.06835836813894502 1103 34-35 0.1047158739170655 1103 36-37 0.14787191889845275 1103 38-39 0.16536966712295254 1103 40-41 0.2605974202306598 1103 42-43 0.15835696702048807 1103 44-45 0.25217519638137986 1103 46-47 0.12327720661513553 1103 48-49 0.14351658382017973 1103 50-51 0.22307888734177084 1103 52-53 0.20248251318830057 1103 54-55 0.10358585891985683 1103 56-57 0.048017655569722706 1103 58-59 0.09089877037365568 1103 60-61 0.07736454415986316 1103 62-63 0.11409198240932028 1103 64-65 0.04951225877180576 1103 66-67 0.2313806563711509 1103 68-69 0.0885616608469455 1103 70-71 0.17759210687050953 1103 72-73 0.038759496539775284 1103 74-75 0.08361129735131456 1103 76-77 0.04390817337774422 1103 78-79 0.025748508937454062 1103 80-81 0.07776727129069272 1103 82-83 0.33020508740778 1103 84-85 0.18258220635606293 1103 86-87 0.1328295866217566 1103 88-89 0.15596419304177545 1103 90-91 0.05315847977232124 1103 92-93 0.2328352573047141 1103 94-95 0.5193322374824589 1103 96-97 0.36694192971463835 1103 98-99 0.5848531194741433 1103 100-101 0.8155982539424187 1104 1 0.21256754081247564 1104 2 0.30750639066630114 1104 3 0.1932222495832434 1104 4 0.11674046881981326 1104 5 0.08893269901040668 1104 6 0.1917177455989929 1104 7 0.22086542367009088 1104 8 0.13049039611787094 1104 9 0.1696360412211746 1104 10-11 0.20998763090410222 1104 12-13 0.21574071563592412 1104 14-15 0.24017288542009396 1104 16-17 0.2650493558285305 1104 18-19 0.2742213807770071 1104 20-21 0.1464515071299104 1104 22-23 0.15295522441956422 1104 24-25 0.2186410272940762 1104 26-27 0.265407693080391 1104 28-29 0.2697250228432324 1104 30-31 0.09695527196034703 1104 32-33 0.2472035065654552 1104 34-35 0.2729289368794312 1104 36-37 0.4058340570276968 1104 38-39 0.3661335708681932 1104 40-41 0.36811322672441094 1104 42-43 0.20827992764026249 1104 44-45 0.3151095194083382 1104 46-47 0.22570633078595392 1104 48-49 0.33645315833604883 1104 50-51 0.06913593734645929 1104 52-53 0.347735196746747 1104 54-55 0.2441877355879427 1104 56-57 0.23282443215071424 1104 58-59 0.22467326733414694 1104 60-61 0.27038885463137063 1104 62-63 0.30815350013718756 1104 64-65 0.46104356768810106 1104 66-67 0.09607658918864104 1104 68-69 0.5009174473737481 1104 70-71 0.2025443729019507 1104 72-73 0.2348129008951645 1104 74-75 0.4592205414359505 1104 76-77 0.35820681054590153 1104 78-79 0.3245839843902161 1104 80-81 0.4426695238212126 1104 82-83 0.47789114171753866 1104 84-85 0.6285801247303766 1104 86-87 0.2969094447699412 1104 88-89 0.360866910292426 1104 90-91 0.5169171201614695 1104 92-93 0.6493987641148671 1104 94-95 0.8260142719630892 1104 96-97 0.8250245745811249 1104 98-99 0.8503517361305128 1104 100-101 1.0096012810331985 1105 1 0.03358171739485982 1105 2 0.21170907807894324 1105 3 0.08282436362202361 1105 4 0.09754748143354419 1105 5 -0.09125854064556904 1105 6 -0.04355288143931091 1105 7 -0.011209658889008267 1105 8 0.11810180673424497 1105 9 -0.01779098261681611 1105 10-11 0.06582987338914847 1105 12-13 -0.06224166244407314 1105 14-15 0.13921701581539736 1105 16-17 0.03706176002998518 1105 18-19 0.05449010560767675 1105 20-21 0.16937577954576 1105 22-23 0.018928879460176518 1105 24-25 0.06217479497166778 1105 26-27 0.1566954999705743 1105 28-29 0.12185159236994281 1105 30-31 0.12032325587498605 1105 32-33 0.08720278348005195 1105 34-35 0.07121974375235851 1105 36-37 -0.0022174167929094324 1105 38-39 0.16817086399797887 1105 40-41 0.19267997120491742 1105 42-43 0.0971651189333258 1105 44-45 0.08757015439235971 1105 46-47 0.16726459626840295 1105 48-49 0.05502133419699362 1105 50-51 0.1389284693532602 1105 52-53 0.14201370130752622 1105 54-55 0.11893249876514744 1105 56-57 0.022318658560259053 1105 58-59 0.1556913305005807 1105 60-61 0.1373641472960756 1105 62-63 0.21340425054372503 1105 64-65 -0.04837667393746159 1105 66-67 0.02005730581558396 1105 68-69 0.21002347552313694 1105 70-71 0.08443453458549044 1105 72-73 0.14181179243810504 1105 74-75 -0.14644195680769556 1105 76-77 -0.2183005554758246 1105 78-79 -0.24493656890159343 1105 80-81 -0.04429604005804322 1105 82-83 -0.06779306552202513 1105 84-85 -0.03843206172377123 1105 86-87 -0.057715877845293306 1105 88-89 -0.036680515482444775 1105 90-91 0.10147209807038848 1105 92-93 -0.011555527848944536 1105 94-95 -0.026430290804420054 1105 96-97 0.22310691921842007 1105 98-99 0.4020613811859626 1105 100-101 0.6243491830584134 1106 1 0.051872943924070825 1106 2 0.043495028095254185 1106 3 0.022821846716382765 1106 4 0.014897950271425486 1106 5 0.02289853994934532 1106 6 -0.048168064544192646 1106 7 -0.007364957103803249 1106 8 -0.0713312987934458 1106 9 0.05942922351261615 1106 10-11 0.10338056463930201 1106 12-13 0.09877866028487148 1106 14-15 0.22124245710857338 1106 16-17 -0.0031997413299151845 1106 18-19 0.04407705057219857 1106 20-21 0.04900476710761836 1106 22-23 0.06635585709310021 1106 24-25 -0.11048236395551925 1106 26-27 0.08173408824332995 1106 28-29 0.16001224799818203 1106 30-31 0.1603163729085253 1106 32-33 0.17449831699984486 1106 34-35 0.14657923749476964 1106 36-37 0.09614094434196119 1106 38-39 0.20790586978942827 1106 40-41 0.29707890588608166 1106 42-43 0.24694925575025906 1106 44-45 0.3990590581772224 1106 46-47 0.34228841698253376 1106 48-49 0.37395437166549783 1106 50-51 0.23175734288388838 1106 52-53 -0.02961358092234434 1106 54-55 0.09741552377754203 1106 56-57 0.13972203378209258 1106 58-59 -0.0140983537818542 1106 60-61 -0.06266821291935543 1106 62-63 0.15370350548828782 1106 64-65 0.14301602241328482 1106 66-67 0.012614456267471041 1106 68-69 -0.04273420553355223 1106 70-71 0.1277381530225945 1106 72-73 -0.004164882524076319 1106 74-75 0.1129636939023051 1106 76-77 -0.1713602652098274 1106 78-79 -0.12211414173133761 1106 80-81 -0.19840771362051868 1106 82-83 0.19688652982118882 1106 84-85 -0.05440157120967726 1106 86-87 -0.10932528490855375 1106 88-89 -0.20135677207327163 1106 90-91 0.05108904044095297 1106 92-93 -0.006442100157329378 1106 94-95 0.07529892610404687 1106 96-97 0.054133945134839934 1106 98-99 0.5189215670774239 1106 100-101 0.7371874564611431 1107 1 -0.004227599022804895 1107 2 0.11054094703808559 1107 3 -0.033502019196880894 1107 4 0.036145656818248995 1107 5 -0.03422962872864588 1107 6 0.050450847283478595 1107 7 0.10928445021178135 1107 8 -0.05252824187613214 1107 9 0.02681783551503969 1107 10-11 -0.02358817751392195 1107 12-13 0.07372128498722219 1107 14-15 0.023601206749361836 1107 16-17 0.03385259956522191 1107 18-19 -0.06017920743044414 1107 20-21 -0.0349312264896966 1107 22-23 -0.07995455276122243 1107 24-25 0.0013565306298062296 1107 26-27 -0.007976844549489215 1107 28-29 -0.06149678609597942 1107 30-31 -0.1146172962027876 1107 32-33 0.03668622457134063 1107 34-35 -0.13732277586947106 1107 36-37 -0.008836012015407846 1107 38-39 0.010631203512723175 1107 40-41 3.199512869613841E-4 1107 42-43 -0.1714791967292726 1107 44-45 0.04479033036427893 1107 46-47 0.20183570051938204 1107 48-49 0.07997618476458257 1107 50-51 0.14456842922541568 1107 52-53 0.17286085454949784 1107 54-55 0.2537183610561513 1107 56-57 -0.0806495552288311 1107 58-59 -0.05785916921676204 1107 60-61 0.16823894877611423 1107 62-63 0.17927285152041605 1107 64-65 0.11267754074872727 1107 66-67 0.10949935595068183 1107 68-69 0.24592744940298772 1107 70-71 0.08532124305292754 1107 72-73 0.015797158239735154 1107 74-75 0.04132803455258127 1107 76-77 -0.055149285088408107 1107 78-79 0.07652781090492056 1107 80-81 0.2929804653394008 1107 82-83 0.34606428753390617 1107 84-85 0.2882066086457975 1107 86-87 0.32163787757137285 1107 88-89 0.39212372769405235 1107 90-91 0.3419897348393448 1107 92-93 0.4847825542447133 1107 94-95 0.7061527226794979 1107 96-97 0.8725805922012349 1107 98-99 1.1048439447755065 1107 100-101 1.1256301245244273 1108 1 -0.10387225438083192 1108 2 0.030795477224646817 1108 3 0.07050149992777222 1108 4 -0.024282381315515522 1108 5 -0.06806688179786846 1108 6 0.012847352870487327 1108 7 -0.00706575074213589 1108 8 0.04464009835778171 1108 9 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-1.6629776703630625 2316 56-57 -1.5084428421260512 2316 58-59 -1.51978934498009 2316 60-61 -1.5129361383425532 2316 62-63 -1.3891308106379583 2316 64-65 -1.69367322524905 2316 66-67 -2.4882129017027452 2316 68-69 -2.008256663809636 2316 70-71 -1.7397710302215792 2316 72-73 -1.4956494164516734 2316 74-75 -1.2946142478371279 2316 76-77 -1.3084646869933358 2316 78-79 -1.4807140903933984 2316 80-81 -2.0893558933885714 2316 82-83 -1.7701994558983394 2316 84-85 -1.7740783612798445 2316 86-87 -1.857865602807049 2316 88-89 -1.74939875075707 2316 90-91 -1.9484980947569497 2316 92-93 -2.225326764176568 2316 94-95 -2.238311638024115 2316 96-97 -2.0522553486250494 2316 98-99 -2.376748578075123 2316 100-101 -2.189960928244176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 24.0 5 63.0 6 153.0 7 311.0 8 795.0 9 1513.0 10 2445.0 11 3857.0 12 5742.0 13 7835.0 14 10162.0 15 13388.0 16 17104.0 17 22159.0 18 19188.0 19 12691.0 20 11080.0 21 11433.0 22 12147.0 23 13879.0 24 16059.0 25 19342.0 26 23590.0 27 29314.0 28 36013.0 29 44691.0 30 54807.0 31 67403.0 32 83893.0 33 105816.0 34 138553.0 35 186593.0 36 260813.0 37 335610.0 38 196381.0 39 10242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.628984174322525 19.329677005598327 14.833599893601313 22.207738926477834 2 15.910805382550372 20.933354741844344 39.20298367037653 23.95285620522875 3 19.630563574208406 25.019267301698907 30.254846684108642 25.095322439984045 4 13.031422561284078 17.035323199785477 36.66031207361413 33.272942165316316 5 13.24222829889572 37.996184990766686 33.92062617528987 14.84096053504772 6 31.556363021801587 36.332037631682724 17.100952059038928 15.010647287476761 7 28.463950019857982 33.540156779458115 20.176496601609482 17.819396599074423 8 27.16013982271471 34.12778399024283 19.30871694913822 19.403359237904237 9 26.983217563251028 16.31682130845544 20.241624273972295 36.45833685432124 10-11 25.308497138889553 26.760586390325038 26.51474073959318 21.41617573119223 12-13 26.611751079383104 24.148098573141162 27.55702815860397 21.683122188871764 14-15 23.195607821574935 25.185338335592878 26.48999515228883 25.12905869054336 16-17 21.77386779819713 28.768170522782622 27.25092518051513 22.20703649850512 18-19 21.60373611701843 26.5862301103315 30.451578920683563 21.358454851966506 20-21 22.470135569445187 25.62963881009856 30.540531405184485 21.359694215271773 22-23 22.171833157613207 25.703990785749237 30.423759201364 21.70041685527356 24-25 22.09137741583025 25.869611571479396 30.694863946220146 21.344147066470207 26-27 21.884047438365446 26.01653841451838 30.560658787314388 21.53875535980178 28-29 21.52650489224663 26.113033643614585 30.895026908412802 21.465434555725977 30-31 21.610329426663753 25.955250568423804 30.709419948577082 21.72500005633536 32-33 21.373902383926062 26.149838572201972 30.593652065211085 21.882606978660885 34-35 22.15837673843415 26.015735698852637 30.298769821036707 21.527117741676502 36-37 22.29429827032509 25.728998816834753 30.20973012564088 21.76697278719928 38-39 21.937646413234912 25.8928742460317 30.46029109683584 21.709188243897547 40-41 22.044892852173238 25.915442255549216 30.485203127086187 21.554461765191363 42-43 21.853950956441498 26.19267526733069 30.016751211413695 21.93662256481412 44-45 21.952776292714198 26.295812646507233 29.68587947718501 22.065531583593557 46-47 22.37942745310344 26.507130429296094 29.03726458767052 22.076177529929943 48-49 22.553269039988326 26.743810042628752 28.572333956587066 22.130586960795856 50-51 22.519481434013816 26.950643278148263 28.457526741639494 22.072348546198427 52-53 22.68480948157095 27.0691853732639 28.285901723571172 21.960103421593978 54-55 22.214341386714693 26.90389546444145 28.465962818543574 22.415800330300275 56-57 22.520108220322154 27.27864761215324 27.963066505359613 22.238177662164997 58-59 22.6884604420949 26.788186991787477 28.079780563733525 22.443572002384098 60-61 22.43093455772061 26.870361893762805 28.627451532714097 22.071252015802482 62-63 22.614200279037775 26.823428468895006 28.585231166710845 21.977140085356375 64-65 22.424439270001685 26.842221499828444 28.487636080301392 22.245703149868476 66-67 22.269378727517992 27.14023227966853 28.17301514288121 22.41737384993227 68-69 22.317781049662955 27.018253795444707 28.470828095234207 22.193137059658135 70-71 22.29143886298429 27.03169900563517 28.27461279711656 22.40224933426398 72-73 22.339421386595653 27.118169037769103 28.008603514698 22.53380606093724 74-75 22.305758198372185 27.505201358634874 27.73094567544987 22.45809476754307 76-77 22.472626973912703 27.181736724881617 28.002976801622708 22.34265949958297 78-79 22.42340676350003 27.058169029755625 27.954344755125582 22.564079451618767 80-81 22.442277784929452 27.523596060126255 27.701057699539415 22.333068455404874 82-83 22.430143473550604 27.389408973702434 27.71616383037817 22.46428372236879 84-85 22.30538529988144 27.275649124111666 27.561419505567947 22.857546070438946 86-87 22.587622686408412 27.165286239179203 27.58358337267486 22.663507701737526 88-89 22.568067846150637 27.16857366122109 27.52018781300054 22.74317067962773 90-91 22.294526683726765 27.188148979492954 27.834917082745974 22.682407254034306 92-93 22.35189665405038 27.06645563469724 27.785012167012706 22.796635544239674 94-95 22.09511946832609 26.857374316436562 27.82432046456681 23.223185750670535 96-97 22.25120353018621 26.7806908999851 27.78230180376719 23.185803766061504 98-99 22.05136684458599 26.87955390690601 27.530265955064504 23.5388132934435 100-101 21.872334552092077 27.025768578559234 27.61277274186915 23.489124127479535 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 600.0 1 464.5 2 498.5 3 648.5 4 535.5 5 481.0 6 771.0 7 1558.5 8 2397.0 9 2864.5 10 2695.5 11 2262.0 12 2308.0 13 2511.5 14 2646.0 15 2848.5 16 3036.0 17 3223.5 18 3493.0 19 3651.5 20 3880.5 21 4353.5 22 4987.5 23 5848.0 24 6877.0 25 8336.0 26 10451.5 27 12787.5 28 14990.0 29 17848.5 30 21110.0 31 24429.0 32 29021.5 33 32408.0 34 34941.5 35 38062.5 36 42371.0 37 46820.0 38 48773.5 39 49554.0 40 50431.5 41 52869.0 42 54978.5 43 57101.5 44 60208.0 45 62599.5 46 62665.0 47 64577.5 48 74838.5 49 74574.5 50 64582.0 51 59467.5 52 56114.5 53 56048.0 54 55849.5 55 52602.5 56 48234.5 57 44336.5 58 40395.5 59 35810.5 60 30696.5 61 25748.0 62 21601.5 63 17156.0 64 12870.0 65 10053.5 66 7537.5 67 5885.5 68 4440.0 69 2975.5 70 2282.5 71 1814.0 72 1525.5 73 1368.0 74 1087.0 75 869.5 76 647.5 77 394.0 78 260.0 79 193.5 80 128.0 81 89.0 82 60.5 83 36.0 84 17.5 85 12.0 86 10.5 87 6.5 88 4.5 89 2.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03644854811405523 2 0.0061968165263463285 3 0.00433777156844243 4 6.196816526346328E-4 5 6.196816526346328E-4 6 2.8167347847028767E-4 7 0.0 8 0.0 9 0.0019717143492920136 10-11 4.2251021770543155E-4 12-13 9.576898267989781E-4 14-15 0.0022815551756093302 16-17 0.0025913960019266465 18-19 0.0 20-21 0.0 22-23 0.0016055388272806397 24-25 9.295224789519493E-4 26-27 0.004844783829688948 28-29 0.0057743063086408974 30-31 0.001183028609575208 32-33 4.506775655524603E-4 34-35 0.009013551311049205 36-37 0.011548612617281795 38-39 0.009182555398131379 40-41 0.0019153796535979562 42-43 5.351796090935466E-4 44-45 0.0012393633052692656 46-47 8.45020435410863E-5 48-49 6.760163483286904E-4 50-51 9.858571746460068E-4 52-53 0.0022815551756093302 54-55 0.003013906219632078 56-57 0.0012675306531162946 58-59 6.196816526346328E-4 60-61 0.003802591959348884 62-63 0.004309604220595401 64-65 0.00799952678855617 66-67 0.0026195633497736754 68-69 0.002788567436855848 70-71 1.1266939138811507E-4 72-73 9.295224789519493E-4 74-75 0.004957453221077063 76-77 0.0025632286540796176 78-79 0.0035209184808785957 80-81 0.0041406001335132295 82-83 0.0041687674813602575 84-85 0.004422273611983516 86-87 0.0028730694803969343 88-89 0.008337534962720515 90-91 0.01143594322589368 92-93 0.0010421918703400644 94-95 0.0018027102622098412 96-97 8.168530875638343E-4 98-99 0.002788567436855848 100-101 0.004225102177054315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1775105.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.23282450948176 #Duplication Level Percentage of deduplicated Percentage of total 1 76.28448813689083 40.60838769786753 2 13.577229196084195 14.455085182403241 3 4.385908659380481 7.004229179382524 4 1.9098972351131196 4.066768973916845 5 0.9984270750067988 2.6574546634676053 6 0.6213640907157264 1.984617935853838 7 0.387747117091637 1.444861196673761 8 0.2912074216540185 1.2401434858213618 9 0.20598789005552143 0.9868785482163596 >10 1.1586357153917888 11.550343509528336 >50 0.11240097972904577 4.154573585327551 >100 0.06118349568292712 6.086907606386131 >500 0.004142240402875583 1.4915059658844825 >1k 0.0010621129238142521 0.9191589267393491 >5k 3.186338771442756E-4 1.34908354253107 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9527 0.5367006458772862 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8879 0.5001957630675369 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5455 0.30730576501108386 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2386 0.13441458392602126 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2341 0.1318795226197887 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2289 0.1289501184436977 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2214 0.12472501626664338 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.633469569405754E-5 0.0 0.0 0.0 0.0 6 5.633469569405754E-5 0.0 0.0 0.0 0.0 7 5.633469569405754E-5 0.0 0.0 0.0 0.0 8 5.633469569405754E-5 0.0 0.0 0.0 0.0 9 5.633469569405754E-5 0.0 0.0 0.0 0.0 10-11 5.633469569405754E-5 0.0 0.0 0.0 0.0 12-13 1.1266939138811507E-4 0.0 0.0 0.0 0.0 14-15 1.1266939138811507E-4 0.0 0.0 5.633469569405754E-5 0.0 16-17 1.1266939138811507E-4 0.0 0.0 8.45020435410863E-5 0.0 18-19 1.1266939138811507E-4 0.0 0.0 1.1266939138811507E-4 0.0 20-21 1.1266939138811507E-4 0.0 0.0 1.4083673923514383E-4 0.0 22-23 1.1266939138811507E-4 0.0 0.0 1.9717143492920138E-4 0.0 24-25 1.1266939138811507E-4 0.0 0.0 2.2533878277623015E-4 0.0 26-27 1.1266939138811507E-4 0.0 0.0 3.0984082631731647E-4 0.0 28-29 1.1266939138811507E-4 0.0 0.0 9.013551311049206E-4 0.0 30-31 1.1266939138811507E-4 0.0 0.0 0.0020843837406801288 0.0 32-33 1.1266939138811507E-4 0.0 0.0 0.004675779742606776 0.0 34-35 1.690040870821726E-4 0.0 0.0 0.006816498178980962 0.0 36-37 1.690040870821726E-4 0.0 0.0 0.010112077877083327 0.0 38-39 1.690040870821726E-4 0.0 0.0 0.014337180054137642 0.0 40-41 1.690040870821726E-4 0.0 0.0 0.019829812884308254 0.0 42-43 1.9717143492920138E-4 0.0 0.0 0.027068821280994644 0.0 44-45 2.2533878277623015E-4 0.0 0.0 0.03244878471977714 0.0 46-47 2.2533878277623015E-4 0.0 0.0 0.03982862985569868 0.0 48-49 2.2533878277623015E-4 0.0 0.0 0.04644795659975044 0.0 50-51 2.2533878277623015E-4 0.0 0.0 0.056813540607457025 0.0 52-53 2.2533878277623015E-4 0.0 0.0 0.07855873314536324 0.0 54-55 2.2533878277623015E-4 0.0 0.0 0.10678241568808605 0.0 56-57 2.2533878277623015E-4 0.0 0.0 0.1338512369690807 0.0 58-59 2.2533878277623015E-4 0.0 0.0 0.17615859343531792 0.0 60-61 2.2533878277623015E-4 0.0 0.0 0.2335073136518685 0.0 62-63 2.2533878277623015E-4 0.0 0.0 0.2703220372879351 0.0 64-65 2.2533878277623015E-4 0.0 0.0 0.30139062196320776 0.0 66-67 2.8167347847028767E-4 0.0 0.0 0.38561099202582383 0.0 68-69 3.380081741643452E-4 0.0 0.0 0.4794645950521237 0.0 70-71 3.380081741643452E-4 0.0 0.0 0.5175468493413066 0.0 72-73 3.380081741643452E-4 0.0 0.0 0.5913453007005219 0.0 74-75 3.380081741643452E-4 0.0 0.0 0.6965785122570214 0.0 76-77 3.380081741643452E-4 0.0 0.0 0.8092760709929836 0.0 78-79 3.380081741643452E-4 0.0 0.0 0.9040873638460823 0.0 80-81 3.9434286985840276E-4 0.0 0.0 1.0405299968170898 0.0 82-83 3.9434286985840276E-4 0.0 0.0 1.1999571856312725 0.0 84-85 4.2251021770543155E-4 0.0 0.0 1.364003819492368 0.0 86-87 4.506775655524603E-4 0.0 0.0 1.608637235543813 0.0 88-89 4.506775655524603E-4 0.0 0.0 1.9110418820295139 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10870 0.0 28.80726 1 GTACACG 350 0.0 24.437151 1 GTATCAA 14535 0.0 24.354956 1 GTACATG 9760 0.0 23.953081 1 TACCTGG 1530 0.0 23.283962 2 TACATGG 10005 0.0 23.263016 2 CATGGGG 3825 0.0 22.848042 4 ACATGGG 9820 0.0 22.829967 3 GGACCGA 175 4.054802E-6 21.712122 6 GTACCTG 1695 0.0 21.585749 1 GAGTACT 10080 0.0 20.89933 12-13 GTACTTT 10495 0.0 20.140238 14-15 ATCAACG 17665 0.0 20.031681 3 TCAACGC 17740 0.0 19.973207 4 TATCAAC 17745 0.0 19.968704 2 CGCGGAA 215 0.0 19.88538 82-83 CAACGCA 17990 0.0 19.668692 5 AACGCAG 18465 0.0 19.21417 6 AGTACTT 10480 0.0 19.036509 12-13 TATCGCG 75 7.7239925E-4 19.000515 90-91 >>END_MODULE