FastQCFastQC Report
Thu 26 May 2016
SRR936472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807585
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74980.4148076024087387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64490.3567743702232537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36230.20043317465015476No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24320.1345441569829358No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20720.11462808111375121No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19860.10987035187833491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG102350.027.628681
TAGACCG1405.775928E-727.1607075
TACATGG105950.026.465622
ACATGGG106550.025.335993
GGTATCA102500.024.8989721
TACCTGG14900.024.2414342
GTACCTG15950.023.5395091
GTATCAA136900.023.5026171
GAGTACT68850.023.05863812-13
CATGGGA62550.022.6435224
CATGGGG41000.022.373224
GTACTTT72150.021.8030214-15
ATGGGAG25300.021.2293645
AGTACTT71800.020.25760712-13
TCAACGC160550.019.9232334
ATCAACG160150.019.8537353
CAACGCA162800.019.7073615
TATCAAC162350.019.5841542
AACGCAG165800.019.3507736
GTCTTAG6250.019.0103871
Produced by FastQC (version 0.11.5)