##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936472_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1807585 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.277993012776715 31.0 31.0 34.0 28.0 34.0 2 31.317416331735437 31.0 31.0 34.0 28.0 34.0 3 31.249527408116354 31.0 31.0 34.0 27.0 34.0 4 34.91574061524078 37.0 35.0 37.0 32.0 37.0 5 34.95786532860142 37.0 35.0 37.0 32.0 37.0 6 35.02866144607307 37.0 35.0 37.0 32.0 37.0 7 34.895892032739816 37.0 35.0 37.0 32.0 37.0 8 34.88116298818589 37.0 35.0 37.0 32.0 37.0 9 36.34556881142519 39.0 37.0 39.0 32.0 39.0 10-11 36.21642135777847 39.0 35.0 39.0 32.0 39.0 12-13 36.110875283873234 39.0 35.0 39.0 31.0 39.0 14-15 37.07985101668801 39.0 36.0 41.0 31.0 41.0 16-17 36.99314748684017 39.0 36.0 41.0 31.0 41.0 18-19 36.88195714171118 39.0 36.0 41.0 30.0 41.0 20-21 36.876650337328535 39.0 36.0 41.0 30.0 41.0 22-23 36.87845246558253 39.0 36.0 41.0 30.5 41.0 24-25 36.985187418572295 39.0 36.0 41.0 31.0 41.0 26-27 36.914156180760514 39.0 36.0 41.0 30.0 41.0 28-29 36.80187017484655 39.0 36.0 41.0 30.0 41.0 30-31 36.600148540732526 39.0 36.0 41.0 30.0 41.0 32-33 36.42651410583734 39.0 35.0 41.0 30.0 41.0 34-35 36.23054213218189 39.0 35.0 40.0 30.0 41.0 36-37 36.0167217585895 38.5 35.0 40.0 29.0 41.0 38-39 35.82006268031655 38.0 35.0 40.0 28.5 41.0 40-41 35.57982529175668 38.0 35.0 40.0 27.5 41.0 42-43 35.29637444435531 38.0 34.0 40.0 26.5 41.0 44-45 35.123047325575286 38.0 34.0 40.0 26.0 41.0 46-47 34.926756971318085 38.0 34.0 40.0 24.5 41.0 48-49 34.6665108418138 38.0 34.0 40.0 24.0 41.0 50-51 33.669081398661746 37.5 32.5 39.5 23.0 40.5 52-53 33.76802529341636 37.5 33.0 39.5 23.0 40.5 54-55 34.20049015675612 38.0 33.5 40.0 23.0 41.0 56-57 34.06129531944556 38.0 33.0 40.0 22.5 41.0 58-59 33.83101430914729 38.0 33.0 40.0 22.0 41.0 60-61 33.544816979561126 37.0 33.0 40.0 21.5 41.0 62-63 33.19359891789321 37.0 32.5 40.0 20.0 41.0 64-65 32.82820144004293 36.0 32.0 39.0 18.5 41.0 66-67 32.47436828696853 36.0 32.0 39.0 18.0 41.0 68-69 32.05095555672347 35.0 31.0 39.0 15.5 40.0 70-71 31.65008146228255 35.0 31.0 38.0 13.0 40.0 72-73 31.234470578147086 35.0 31.0 37.0 9.0 39.5 74-75 30.821660668792894 35.0 30.0 37.0 7.5 39.0 76-77 30.36448770044009 34.5 30.0 36.0 6.5 39.0 78-79 29.905588672178624 34.0 29.0 36.0 2.0 38.5 80-81 29.4705510390936 34.0 29.0 35.0 2.0 37.0 82-83 29.081113197996224 34.0 29.0 35.0 2.0 37.0 84-85 28.709219483454444 34.0 29.0 35.0 2.0 36.0 86-87 28.349794338855435 34.0 28.0 35.0 2.0 36.0 88-89 27.99128671680723 34.0 27.0 35.0 2.0 36.0 90-91 27.635254220410104 34.0 26.5 35.0 2.0 35.0 92-93 27.221790676510373 33.0 26.0 35.0 2.0 35.0 94-95 26.739893006414636 33.0 25.0 35.0 2.0 35.0 96-97 26.139148366466863 33.0 24.5 35.0 2.0 35.0 98-99 25.389977234818833 32.5 21.0 35.0 2.0 35.0 100-101 22.77534970692941 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7947968828865406 1101 2 0.6193388790217682 1101 3 0.8120372019168123 1101 4 0.8209799957248549 1101 5 0.6545706882311819 1101 6 0.6609923438226701 1101 7 0.7689265880596494 1101 8 0.7532632189055022 1101 9 1.053657749766593 1101 10-11 1.0031298495679692 1101 12-13 1.0512879198739924 1101 14-15 1.3330821171599538 1101 16-17 1.3866871659669826 1101 18-19 1.4680158199698141 1101 20-21 1.3259399277575667 1101 22-23 1.305002281577913 1101 24-25 1.2498196413641764 1101 26-27 1.1672540344385922 1101 28-29 1.1360212399282332 1101 30-31 1.0096982656255449 1101 32-33 0.9513929424828262 1101 34-35 0.937570676850946 1101 36-37 0.9058270719098687 1101 38-39 1.045328746596219 1101 40-41 0.9391830502189364 1101 42-43 1.0067988363197529 1101 44-45 1.0260478747309492 1101 46-47 1.1468402392794914 1101 48-49 1.1963186851768128 1101 50-51 1.0798466778465112 1101 52-53 1.3184039830119758 1101 54-55 1.2828020713695452 1101 56-57 1.2888065025803783 1101 58-59 1.24832159651654 1101 60-61 1.23591795599868 1101 62-63 1.1269190985106334 1101 64-65 0.8643140038521935 1101 66-67 0.9893394198720884 1101 68-69 1.0461291284880119 1101 70-71 1.107076528138034 1101 72-73 1.203295720108855 1101 74-75 0.9782556531996462 1101 76-77 1.0583475166029466 1101 78-79 1.196604978174328 1101 80-81 1.0899135953316836 1101 82-83 1.075244847911808 1101 84-85 1.0511744410246955 1101 86-87 1.1184658682760826 1101 88-89 1.1191951130467928 1101 90-91 1.1681995125149491 1101 92-93 1.150135582649824 1101 94-95 1.100268276256017 1101 96-97 1.1861031949032004 1101 98-99 1.2509592044004627 1101 100-101 1.534883232581766 1102 1 0.5950139061280417 1102 2 0.7424156837008518 1102 3 0.8075582116135998 1102 4 0.6060023137177737 1102 5 0.5986802772722797 1102 6 0.5531810463007361 1102 7 0.5798870313411655 1102 8 0.47058659892704924 1102 9 0.635200000035951 1102 10-11 0.6414267558323985 1102 12-13 0.5050365730985575 1102 14-15 0.8005270917636125 1102 16-17 0.6176822406168228 1102 18-19 0.7309256244949793 1102 20-21 0.7938705111037265 1102 22-23 0.46501151660946505 1102 24-25 0.7144325396249087 1102 26-27 0.7282621920498045 1102 28-29 0.6458957974163084 1102 30-31 0.5777827661642547 1102 32-33 0.7480698702956587 1102 34-35 0.5662500673388564 1102 36-37 0.5015520218867806 1102 38-39 0.45515251141075197 1102 40-41 0.7344016126323538 1102 42-43 0.6128608649483454 1102 44-45 0.6752744408383791 1102 46-47 0.6584225080189086 1102 48-49 0.7589914572254486 1102 50-51 0.7620546199736467 1102 52-53 0.5692689976341114 1102 54-55 0.7587301920406233 1102 56-57 0.5808282049045275 1102 58-59 0.46933668040140475 1102 60-61 0.6337631153029761 1102 62-63 0.6701936867821416 1102 64-65 0.7915619644493859 1102 66-67 0.6642393736969368 1102 68-69 0.5439334997362764 1102 70-71 0.29282148734997904 1102 72-73 0.5983719091191624 1102 74-75 0.42614160055996564 1102 76-77 0.6530073596074111 1102 78-79 0.5889445195010943 1102 80-81 0.8636761011035823 1102 82-83 0.7146045523907993 1102 84-85 0.6979768113494593 1102 86-87 0.46348587751111125 1102 88-89 0.6449239109535156 1102 90-91 0.7885773792226622 1102 92-93 0.7390850978106656 1102 94-95 0.8903475436101758 1102 96-97 0.865815369753129 1102 98-99 0.9455913423102693 1102 100-101 1.4325629309040728 1103 1 0.19240381735151146 1103 2 0.30107080745885995 1103 3 0.40481701577609996 1103 4 0.357268996062416 1103 5 0.20693120325263692 1103 6 0.27497098104117157 1103 7 0.2790510033559812 1103 8 0.1346732589305759 1103 9 0.18126179085257377 1103 10-11 0.06559307954337612 1103 12-13 0.17764901357124785 1103 14-15 0.4135162445650735 1103 16-17 0.6059413975337478 1103 18-19 0.7112808775962733 1103 20-21 0.6977300854467217 1103 22-23 0.5193431939567432 1103 24-25 0.37970945184785165 1103 26-27 0.29981901490828733 1103 28-29 0.3750981314747506 1103 30-31 0.3188226568103829 1103 32-33 0.3669755966758217 1103 34-35 0.3067772882219231 1103 36-37 0.448729251588702 1103 38-39 0.24144800203335848 1103 40-41 0.44090581628923786 1103 42-43 0.47617543370606796 1103 44-45 0.4791407763078439 1103 46-47 0.6147713527481713 1103 48-49 0.6515074781555441 1103 50-51 0.6174835105609873 1103 52-53 0.7833790276182384 1103 54-55 0.760839516515901 1103 56-57 0.5543397282157159 1103 58-59 0.475588076721003 1103 60-61 0.5852880432822829 1103 62-63 0.6807757315819529 1103 64-65 0.7828358488635203 1103 66-67 0.5982588633788666 1103 68-69 0.6440477039810872 1103 70-71 0.6094931023773178 1103 72-73 0.7306881828565963 1103 74-75 0.5933476385398571 1103 76-77 0.5771285601483029 1103 78-79 0.523162580527611 1103 80-81 0.4694652421465584 1103 82-83 0.4317284279860729 1103 84-85 0.637175911825679 1103 86-87 0.6002442113343811 1103 88-89 0.5107988889063648 1103 90-91 0.6124881029571547 1103 92-93 0.6355547609301908 1103 94-95 0.6294009859054341 1103 96-97 0.894125088137514 1103 98-99 0.9892175108092935 1103 100-101 1.5430889759390531 1104 1 0.300912757001246 1104 2 0.29630576283406285 1104 3 0.43407541244749126 1104 4 0.4120773207510382 1104 5 0.39896247338126045 1104 6 0.41997755780283086 1104 7 0.4784150237235991 1104 8 0.5174865534813549 1104 9 0.5807122018436743 1104 10-11 0.5385359769798015 1104 12-13 0.6613735060192738 1104 14-15 0.7110572193722362 1104 16-17 0.7132725657338597 1104 18-19 0.7164616311891976 1104 20-21 0.808179989055354 1104 22-23 0.6416064079365498 1104 24-25 0.7778145647715249 1104 26-27 0.508035107859854 1104 28-29 0.3835141545905927 1104 30-31 0.2817508243347433 1104 32-33 0.49055755307030324 1104 34-35 0.5896208392664093 1104 36-37 0.5495643138645363 1104 38-39 0.397186170077525 1104 40-41 0.5843950482545637 1104 42-43 0.6447488809913935 1104 44-45 0.7287250673397168 1104 46-47 0.9986614225160864 1104 48-49 0.8419445615990568 1104 50-51 0.5792382854933535 1104 52-53 0.586423520201862 1104 54-55 0.8667958646729375 1104 56-57 0.8911505807764755 1104 58-59 0.9500997352175702 1104 60-61 0.7967104487950039 1104 62-63 0.7551931457619929 1104 64-65 0.8373533326532723 1104 66-67 0.9154162728579891 1104 68-69 0.7338087765582557 1104 70-71 0.576822728089514 1104 72-73 0.8011328431363438 1104 74-75 0.679064624213364 1104 76-77 0.6325591063811267 1104 78-79 0.5646038066529471 1104 80-81 0.7602766190402015 1104 82-83 0.6635479761105678 1104 84-85 0.7792863686507054 1104 86-87 0.7123131622391945 1104 88-89 0.5845997316810809 1104 90-91 0.8007963553239996 1104 92-93 0.732940695926473 1104 94-95 0.8312081263249453 1104 96-97 0.7807494013631349 1104 98-99 0.7888707900368992 1104 100-101 1.441677316990475 1105 1 0.3821948722419535 1105 2 0.40283385241029634 1105 3 0.5221436478068213 1105 4 0.5871775119874201 1105 5 0.6535180566522314 1105 6 0.5197563887664955 1105 7 0.6261235129561626 1105 8 0.5309687186098202 1105 9 0.5428712329126597 1105 10-11 0.6560482410523036 1105 12-13 0.648082715852702 1105 14-15 0.9617131047412641 1105 16-17 1.0021306905086718 1105 18-19 0.8885391789290082 1105 20-21 0.7049375622933454 1105 22-23 0.8137101467464447 1105 24-25 0.6516351351548266 1105 26-27 0.7275556311269469 1105 28-29 0.8357876503362789 1105 30-31 0.6573357582334722 1105 32-33 0.703495248810448 1105 34-35 0.5526091156445503 1105 36-37 0.5774208034296819 1105 38-39 0.43549137226150236 1105 40-41 0.6500937539445388 1105 42-43 0.5016243833333505 1105 44-45 0.659856272128934 1105 46-47 0.7663316644716858 1105 48-49 0.7882524521785896 1105 50-51 0.7327828103125071 1105 52-53 0.7396547222195551 1105 54-55 0.5428109418603739 1105 56-57 0.5938094594106573 1105 58-59 0.6952198815549764 1105 60-61 0.6239073114096811 1105 62-63 0.7844294474166063 1105 64-65 0.8830543941537883 1105 66-67 0.8623287752830961 1105 68-69 0.8281368162467331 1105 70-71 0.9790723885756485 1105 72-73 0.9834908709071968 1105 74-75 0.8974898341576569 1105 76-77 0.9291429039477173 1105 78-79 0.8511939787656928 1105 80-81 1.005428083800048 1105 82-83 0.8426398804369448 1105 84-85 1.0316682316811132 1105 86-87 1.2272890498254583 1105 88-89 1.0900969462815624 1105 90-91 1.0695182588189134 1105 92-93 1.1337606565705798 1105 94-95 1.2180151715842236 1105 96-97 1.1847519234661803 1105 98-99 1.4395783646606652 1105 100-101 1.9597294774073148 1106 1 0.13819221854889818 1106 2 0.09410873078269688 1106 3 0.10983604055339313 1106 4 0.33825999761324965 1106 5 0.2567765705841225 1106 6 0.30122697700178946 1106 7 0.2779059101255541 1106 8 0.32425155806139117 1106 9 0.387703165685771 1106 10-11 0.472026790366769 1106 12-13 0.536760844999101 1106 14-15 0.42651463061477557 1106 16-17 0.3446550160283479 1106 18-19 0.3867540174960098 1106 20-21 0.1347014941818898 1106 22-23 0.06833199548594138 1106 24-25 -0.1314315388832057 1106 26-27 1.2805483327582579E-4 1106 28-29 0.1716434663468931 1106 30-31 0.027841618472301377 1106 32-33 0.0091542492527239 1106 34-35 -0.02992294981320498 1106 36-37 -0.2045655963934081 1106 38-39 0.023708975092112894 1106 40-41 -0.19074271222087447 1106 42-43 -0.1696853247604153 1106 44-45 -0.24252154741993337 1106 46-47 -0.15897129881889072 1106 48-49 -0.22033224485812042 1106 50-51 -0.3221939698732541 1106 52-53 -0.17949222603343173 1106 54-55 -0.11238310944435881 1106 56-57 0.0706460361466128 1106 58-59 -0.10628608925440375 1106 60-61 -0.040542600133882445 1106 62-63 -0.11022446678065734 1106 64-65 -0.2922894802380753 1106 66-67 -0.40566105222686133 1106 68-69 -0.23908178171877026 1106 70-71 -0.21736226423284677 1106 72-73 -0.31446523258683357 1106 74-75 -0.22191253205198436 1106 76-77 -0.272878878449454 1106 78-79 -0.1294053135828257 1106 80-81 -0.11239201792485076 1106 82-83 -0.1043758205803087 1106 84-85 0.09027780709022082 1106 86-87 0.14680419798998656 1106 88-89 0.35073604402592196 1106 90-91 0.427034844400513 1106 92-93 0.3444373482999019 1106 94-95 0.39143235424675993 1106 96-97 0.33459601899912883 1106 98-99 0.592296188632357 1106 100-101 1.1257611005829027 1107 1 0.21816564750917067 1107 2 0.3238404820950471 1107 3 0.30202593707755554 1107 4 0.4679933691109497 1107 5 0.4432596342466013 1107 6 0.4686509903089089 1107 7 0.371845336795964 1107 8 0.35776482197878323 1107 9 0.557935500264847 1107 10-11 0.6110665065102907 1107 12-13 0.5541164054752201 1107 14-15 0.39220086011943067 1107 16-17 0.3848407432755536 1107 18-19 0.7085324139445603 1107 20-21 0.5236616862278609 1107 22-23 0.4659595040729272 1107 24-25 0.4692890385508548 1107 26-27 0.36386736160996946 1107 28-29 0.48644655982078433 1107 30-31 0.311377159938246 1107 32-33 0.5724365287455058 1107 34-35 0.46323284720997293 1107 36-37 0.5928327909821149 1107 38-39 0.7336255895528083 1107 40-41 0.6422700494390554 1107 42-43 0.599462970804602 1107 44-45 0.49498074784264645 1107 46-47 0.5163370764592443 1107 48-49 0.5660112128334376 1107 50-51 0.4955835138708906 1107 52-53 0.44061737950544 1107 54-55 0.4319463768488774 1107 56-57 0.7373644587341559 1107 58-59 0.7783777762918191 1107 60-61 0.7864306506060004 1107 62-63 0.7681765722982163 1107 64-65 0.8220694124192462 1107 66-67 0.763052628618226 1107 68-69 0.8131253157731848 1107 70-71 0.9077329216564216 1107 72-73 0.8060642709883723 1107 74-75 0.7553439810931195 1107 76-77 0.7620829661855701 1107 78-79 0.9417148825676165 1107 80-81 0.7539722591484441 1107 82-83 0.759502957151863 1107 84-85 0.870121756238003 1107 86-87 0.8937489334677267 1107 88-89 0.7089989893068633 1107 90-91 0.6947780342214323 1107 92-93 1.0096319865665215 1107 94-95 0.9692213683099702 1107 96-97 1.2737162043483323 1107 98-99 1.2420029350841517 1107 100-101 1.7626330087291926 1108 1 0.21815489031288493 1108 2 0.276929176557573 1108 3 0.2347526380936955 1108 4 0.24889989734996476 1108 5 0.2283904307204523 1108 6 0.1875246291098378 1108 7 0.45018834241177785 1108 8 0.40988785120525506 1108 9 0.04928754192553697 1108 10-11 0.1388226932772909 1108 12-13 0.15672626679013035 1108 14-15 0.5564883475693705 1108 16-17 0.729868436160352 1108 18-19 0.6286628518451991 1108 20-21 0.6537386691377165 1108 22-23 0.6322723785061015 1108 24-25 0.5470914260967774 1108 26-27 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-4.754601136279636 2316 52-53 -4.7946466285023845 2316 54-55 -4.399837715180453 2316 56-57 -4.875859070320754 2316 58-59 -5.185339037867472 2316 60-61 -5.380283031704742 2316 62-63 -5.074889757675773 2316 64-65 -5.6453790789094 2316 66-67 -5.366758322993576 2316 68-69 -5.512237349255521 2316 70-71 -4.902804649151417 2316 72-73 -3.8543076075899307 2316 74-75 -4.352269519979895 2316 76-77 -4.961175369612288 2316 78-79 -4.996370064084793 2316 80-81 -5.713002284155241 2316 82-83 -5.0438397034786 2316 84-85 -4.855940835233962 2316 86-87 -5.391022547752556 2316 88-89 -7.0435538843347985 2316 90-91 -6.002808243277034 2316 92-93 -5.057511661392468 2316 94-95 -4.506792311725825 2316 96-97 -5.990564797539875 2316 98-99 -7.34018875224665 2316 100-101 -7.771312286293973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4694.0 3 1849.0 4 1918.0 5 1995.0 6 2273.0 7 2738.0 8 3328.0 9 4156.0 10 5392.0 11 6677.0 12 8271.0 13 10052.0 14 11235.0 15 13394.0 16 16284.0 17 20050.0 18 16611.0 19 12738.0 20 12599.0 21 13355.0 22 14787.0 23 17068.0 24 19232.0 25 22306.0 26 25787.0 27 30695.0 28 36256.0 29 43475.0 30 52158.0 31 63619.0 32 78522.0 33 99486.0 34 131021.0 35 175408.0 36 242336.0 37 311622.0 38 239997.0 39 34181.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.946388719568745 18.517884913602433 13.901285707404817 22.63444065942401 2 15.692700343765843 20.21555645573655 39.83162557192852 24.260117628569088 3 19.54433133664062 24.941432683273927 29.581281033484903 25.93295494660055 4 12.60782403096014 16.410168728112183 37.03524792402947 33.94675931689821 5 13.428459333966744 37.9186087241381 33.73151160364929 14.921420338245865 6 31.693633448770864 35.817177059917945 17.54783241968348 14.941357071627714 7 28.830276803965038 33.16398957362135 20.252598289879014 17.753135332534598 8 27.0365163097327 33.914225142395345 19.62409843960168 19.42516010827028 9 27.282093470527073 16.52375959280342 20.22341778949478 35.97072914717472 10-11 25.25878616356553 26.53954898039677 26.79770546025727 21.40395939578043 12-13 26.54203259177922 24.03546863135337 27.3875990958781 22.03489968098931 14-15 23.235451449299628 25.21836420628558 26.086729118243912 25.459455226170885 16-17 22.075508775037434 28.721205394828246 26.720895410853313 22.482390419281003 18-19 21.941088836126603 26.944616110997487 29.407481475925827 21.706813576950083 20-21 22.957876433295475 26.07325801601058 29.294768134326187 21.674097416367754 22-23 22.52156323121195 26.157642172916397 29.21450580653871 22.106288789332947 24-25 22.421208036459337 26.333691906213268 29.427849381683124 21.81725067564427 26-27 22.13862430407569 26.505883088589695 29.38365219085079 21.971840416483825 28-29 21.82079973443616 26.668750605125936 29.622567393739885 21.887882266698018 30-31 21.910425648563738 26.497769658840507 29.46205155980042 22.129753132795337 32-33 21.64063431501133 26.755444358286233 29.301562439052592 22.302358887649845 34-35 22.4536006668214 26.510106753945834 29.120486803968504 21.915805775264257 36-37 22.54247748222492 26.199013950909993 29.08573725329755 22.172771313567537 38-39 22.25450292255297 26.435569803080046 29.24445179689495 22.06547547747204 40-41 22.26247408033602 26.491639317778954 29.347643274607915 21.898243327277115 42-43 22.105526672116024 26.709874263679755 28.873435211425395 22.311163852778826 44-45 22.117557891708653 26.865763708610004 28.629062930911676 22.387615468769663 46-47 22.454633241746464 27.009385787078642 28.078083685117093 22.4578972860578 48-49 22.61491683853664 27.16617023536597 27.77948049533959 22.43943243075779 50-51 22.541961849770782 27.377003697516745 27.759286044841 22.321748407871475 52-53 22.73488962885798 27.360735302334664 27.666447405181394 22.23792766362596 54-55 22.217316955644034 27.272719728742445 27.82038536585636 22.68957794975716 56-57 22.53494087885733 27.691377815152553 27.333096360062903 22.440584945927217 58-59 22.633869962086152 27.115648576861435 27.53309923713112 22.717382223921298 60-61 22.453522487298663 27.16525762011013 28.034862768030973 22.34635712456023 62-63 22.618940922891714 27.28886174327925 27.95631732922608 22.13588000460295 64-65 22.32342290732692 27.36744737215534 27.848672350710856 22.46045736980689 66-67 22.234660607725313 27.619494942537703 27.54848826591848 22.597356183818498 68-69 22.283008142338083 27.50857179213041 27.779770985503678 22.428649080027828 70-71 22.24819976719792 27.483397361281387 27.575207021239873 22.693195850280823 72-73 22.3127858182942 27.4824639089775 27.44588802078794 22.75886225194036 74-75 22.227712390670796 27.878303807232008 27.3315402062004 22.5624435958968 76-77 22.42197008873 27.58050623882367 27.476196128462465 22.521327543983865 78-79 22.344088443096393 27.559783169550332 27.480185710803646 22.615942676549626 80-81 22.35575972102918 28.005555071423334 27.219158356882904 22.419526850664575 82-83 22.380466970308476 27.857720805365418 27.239049461895625 22.52276276243048 84-85 22.283445181492773 27.77370526304142 27.13088724267086 22.81196231279495 86-87 22.503365027940937 27.689159381996642 27.19343668796053 22.61403890210189 88-89 22.492393060258028 27.627519971674523 27.05810595498905 22.821981013078403 90-91 22.28020081130067 27.69169357355151 27.35210249918671 22.676003115961112 92-93 22.393703192223935 27.650116853455142 27.31651462438696 22.639665329933962 94-95 22.12250591266382 27.44247861301868 27.396720999452512 23.03829447486499 96-97 22.20086512829195 27.31973450808416 27.350137935842405 23.129262427781487 98-99 22.050060551093807 27.48098319371674 27.154198195336694 23.314758059852757 100-101 22.171250522904895 27.4479419127363 27.30999462154966 23.070812942809145 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 320.0 1 268.5 2 315.5 3 442.0 4 440.0 5 470.0 6 712.5 7 1248.0 8 1983.0 9 2425.0 10 2266.5 11 2006.5 12 2070.5 13 2207.0 14 2346.0 15 2483.0 16 2588.5 17 2701.5 18 2871.0 19 3069.0 20 3324.0 21 3907.5 22 4570.5 23 5367.5 24 6418.5 25 7907.0 26 10029.5 27 12489.0 28 14876.0 29 17514.0 30 20839.0 31 24353.5 32 28864.0 33 32487.5 34 35451.0 35 38932.5 36 43043.5 37 47647.5 38 49688.0 39 50238.0 40 51237.0 41 53679.0 42 55868.0 43 58210.0 44 61387.5 45 64676.5 46 65204.5 47 66162.5 48 76070.0 49 76141.5 50 66628.5 51 62113.5 52 58575.0 53 57866.0 54 57525.5 55 54289.0 56 49973.5 57 45312.0 58 41490.5 59 37523.0 60 31898.5 61 26826.5 62 22904.5 63 18208.5 64 13446.0 65 10423.5 66 7820.0 67 6078.0 68 4640.5 69 3181.5 70 2438.5 71 1944.5 72 1597.0 73 1390.5 74 1101.0 75 905.0 76 684.5 77 415.0 78 257.5 79 158.5 80 105.0 81 65.5 82 37.5 83 25.5 84 18.0 85 10.5 86 9.0 87 8.5 88 7.5 89 5.0 90 3.5 91 4.0 92 3.0 93 2.5 94 2.0 95 1.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07424270504568249 2 0.02992943623674682 3 0.01454979987109873 4 0.01980543100324466 5 0.024231225640841233 6 0.03026137083456656 7 0.07728543885903015 8 0.055045820805107365 9 0.07097868150045503 10-11 0.062099431008776906 12-13 0.041436502294497905 14-15 0.04417496272651079 16-17 0.05753533028875544 18-19 0.05252865010497432 20-21 0.018947933292210322 22-23 0.012502869851210317 24-25 0.023290744280351963 26-27 0.025227029434300465 28-29 0.006085467626695287 30-31 7.745140615794002E-4 32-33 0.01031763374889701 34-35 0.011313437542356237 36-37 0.0037895866584420654 38-39 0.00970908698622748 40-41 0.004259827338686701 42-43 0.0017979790715236075 44-45 4.1491824727467867E-4 46-47 6.362079791545072E-4 48-49 9.404813604892716E-4 50-51 0.0011341098758841216 52-53 1.106448659399143E-4 54-55 2.212897318798286E-4 56-57 0.0037342642254721073 58-59 0.0036512805760171717 60-61 0.005421598431055801 62-63 0.0027107992155279005 64-65 5.808855461845501E-4 66-67 4.702406802446358E-4 68-69 0.0031810398957725365 70-71 0.0022682197517682433 72-73 0.02121615304397857 74-75 0.017371243952566544 76-77 0.026195172011274713 78-79 0.020275671683489295 80-81 0.012585853500665252 82-83 0.004564100720021465 84-85 0.0041491824727467866 86-87 0.0012170935253390572 88-89 0.0013830608242489289 90-91 0.0064727246574849875 92-93 0.01067722956320173 94-95 0.01302843296442491 96-97 0.012336902552300447 98-99 0.023982274692476425 100-101 0.02063526749779402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1807585.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.819687340756474 #Duplication Level Percentage of deduplicated Percentage of total 1 77.76263881727995 43.4068618557274 2 12.815245128497779 14.306859525357973 3 4.160183210921633 6.9666037834172965 4 1.7801378312251517 3.974669486497611 5 0.9311332929647953 2.5987784642931935 6 0.5468457501474041 1.8314855278109725 7 0.3679841237262903 1.437853111293464 8 0.23379975750604814 1.0440503491465833 9 0.18296241902337387 0.9191614522493884 >10 1.0617707823640843 11.164816903961823 >50 0.09907743970645705 3.875732907649723 >100 0.05411335302818645 5.631273588964747 >500 0.003106119557936256 1.2031635408585584 >1k 8.015792407577437E-4 0.8586354908544341 >5k 2.0039481018943592E-4 0.7800540119168052 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7498 0.4148076024087387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6449 0.3567743702232537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3623 0.20043317465015476 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2432 0.1345441569829358 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2072 0.11462808111375121 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1986 0.10987035187833491 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.532243296995715E-5 0.0 0.0 0.0 0.0 8 5.532243296995715E-5 0.0 0.0 0.0 0.0 9 5.532243296995715E-5 0.0 0.0 0.0 0.0 10-11 5.532243296995715E-5 0.0 0.0 0.0 0.0 12-13 5.532243296995715E-5 0.0 0.0 0.0 0.0 14-15 5.532243296995715E-5 0.0 0.0 0.0 0.0 16-17 5.532243296995715E-5 0.0 0.0 0.0 0.0 18-19 5.532243296995715E-5 0.0 0.0 0.0 0.0 20-21 5.532243296995715E-5 0.0 0.0 2.7661216484978576E-5 0.0 22-23 5.532243296995715E-5 0.0 0.0 1.3830608242489288E-4 0.0 24-25 5.532243296995715E-5 0.0 0.0 2.7661216484978576E-4 0.0 26-27 5.532243296995715E-5 0.0 0.0 4.702406802446358E-4 0.0 28-29 5.532243296995715E-5 0.0 0.0 7.745140615794001E-4 0.0 30-31 5.532243296995715E-5 0.0 0.0 0.0022958809682532217 0.0 32-33 5.532243296995715E-5 0.0 0.0 0.004591761936506443 0.0 34-35 5.532243296995715E-5 0.0 0.0 0.007330222368519322 0.0 36-37 5.532243296995715E-5 0.0 0.0 0.010981502944536495 0.0 38-39 5.532243296995715E-5 0.0 0.0 0.014107220407339072 0.0 40-41 5.532243296995715E-5 0.0 0.0 0.019196884240575134 0.0 42-43 8.298364945493573E-5 0.0 0.0 0.026112188361819777 0.0 44-45 1.6596729890987146E-4 0.0 0.0 0.03355305559627901 0.0 46-47 1.6596729890987146E-4 0.0 0.0 0.04248762852092709 0.0 48-49 1.6596729890987146E-4 0.0 0.0 0.049762528456476454 0.0 50-51 1.6596729890987146E-4 0.0 0.0 0.059250325710824114 0.0 52-53 1.6596729890987146E-4 0.0 0.0 0.08049413997128765 0.0 54-55 1.6596729890987146E-4 0.0 0.0 0.10887454808487568 0.0 56-57 2.7661216484978576E-4 0.0 0.0 0.13515270374560534 0.0 58-59 2.7661216484978576E-4 0.0 0.0 0.1797149235029058 0.0 60-61 2.7661216484978576E-4 0.0 0.0 0.2321605899584252 0.0 62-63 2.7661216484978576E-4 0.0 0.0 0.2687010569350819 0.0 64-65 2.7661216484978576E-4 0.0 0.0 0.2978006566772794 0.0 66-67 2.7661216484978576E-4 0.0 0.0 0.37738197650456273 0.0 68-69 2.7661216484978576E-4 0.0 0.0 0.46401690653551564 0.0 70-71 2.7661216484978576E-4 0.0 0.0 0.5037937358409148 0.0 72-73 3.042733813347643E-4 0.0 0.0 0.5767640249282884 0.0 74-75 3.8725703078970006E-4 0.0 0.0 0.6832320471789708 0.0 76-77 3.8725703078970006E-4 0.0 0.0 0.7966153735508981 0.0 78-79 3.8725703078970006E-4 0.0 0.0 0.8931806802999582 0.0 80-81 4.425794637596572E-4 0.0 0.0 1.0262587928091902 0.0 82-83 4.425794637596572E-4 0.0 0.0 1.177316696033658 0.0 84-85 4.425794637596572E-4 0.0 0.0 1.3426477869643751 0.0 86-87 4.425794637596572E-4 0.0 0.0 1.592041314792942 0.0 88-89 4.425794637596572E-4 0.0 0.0 1.8942677661078178 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10235 0.0 27.62868 1 TAGACCG 140 5.775928E-7 27.160707 5 TACATGG 10595 0.0 26.46562 2 ACATGGG 10655 0.0 25.33599 3 GGTATCA 10250 0.0 24.898972 1 TACCTGG 1490 0.0 24.241434 2 GTACCTG 1595 0.0 23.539509 1 GTATCAA 13690 0.0 23.502617 1 GAGTACT 6885 0.0 23.058638 12-13 CATGGGA 6255 0.0 22.643522 4 CATGGGG 4100 0.0 22.37322 4 GTACTTT 7215 0.0 21.80302 14-15 ATGGGAG 2530 0.0 21.229364 5 AGTACTT 7180 0.0 20.257607 12-13 TCAACGC 16055 0.0 19.923233 4 ATCAACG 16015 0.0 19.853735 3 CAACGCA 16280 0.0 19.707361 5 TATCAAC 16235 0.0 19.584154 2 AACGCAG 16580 0.0 19.350773 6 GTCTTAG 625 0.0 19.010387 1 >>END_MODULE