FastQCFastQC Report
Thu 26 May 2016
SRR936472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807585
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99140.5484666004641552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90850.5026043035320608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54180.29973694183122784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24150.13360367562244652No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23820.13177803533443794No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23760.1314461007366182No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22910.12674369393417184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116300.025.283241
GTATCAA156500.025.011211
GTACATG97000.024.2412661
GGACCGT2551.8189894E-1124.208556
TACATGG100650.022.9329642
GAGTACT97400.022.52478412-13
ACATGGG99550.021.944733
CGTATAG1302.9411181E-421.92451
GTACCTG17450.021.7779181
TACCTGG16800.021.768032
CATGGGG37700.021.539344
GTACTTT103150.021.42969914-15
ATCAACG185750.020.939623
TATCAAC186500.020.932962
TCAACGC187050.020.8685254
CAACGCA189750.020.5215285
AACGCAG192000.020.3052126
GTACCCG1656.3221174E-520.1528261
AGTACTT99950.020.02591712-13
GTACACG2502.1722553E-719.0012341
Produced by FastQC (version 0.11.5)