##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936472_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1807585 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.875713728538354 31.0 31.0 34.0 30.0 34.0 2 32.02747643955886 31.0 31.0 34.0 30.0 34.0 3 32.1039237435584 33.0 31.0 34.0 30.0 34.0 4 35.809737854651374 37.0 35.0 37.0 35.0 37.0 5 35.64627832162803 37.0 35.0 37.0 33.0 37.0 6 35.74617735818786 37.0 35.0 37.0 33.0 37.0 7 35.68716436571447 37.0 35.0 37.0 33.0 37.0 8 35.66223663064254 37.0 35.0 37.0 33.0 37.0 9 37.2808111375122 39.0 37.0 39.0 34.0 39.0 10-11 37.17436828696853 39.0 37.0 39.0 33.0 39.0 12-13 37.06146128674447 39.0 37.0 39.0 33.0 39.0 14-15 38.169580130394976 40.0 37.0 41.0 33.0 41.0 16-17 38.07244140662818 40.0 37.0 41.0 33.0 41.0 18-19 38.24672975268106 40.0 37.5 41.0 33.5 41.0 20-21 38.278022610278356 40.0 38.0 41.0 34.0 41.0 22-23 38.197020610372405 40.0 37.5 41.0 33.0 41.0 24-25 38.05599626020353 40.0 37.0 41.0 33.0 41.0 26-27 37.85885006790829 40.0 37.0 41.0 33.0 41.0 28-29 37.76801478215408 40.0 37.0 41.0 33.0 41.0 30-31 37.70487556601764 40.0 37.0 41.0 33.0 41.0 32-33 37.515677271055026 40.0 36.0 41.0 32.5 41.0 34-35 37.32996373614519 39.0 36.0 41.0 32.0 41.0 36-37 37.11474591789597 39.0 36.0 41.0 31.0 41.0 38-39 36.93393561022027 39.0 35.5 41.0 31.0 41.0 40-41 36.7205329209968 39.0 35.0 41.0 31.0 41.0 42-43 36.42550502465998 39.0 35.0 40.5 30.0 41.0 44-45 36.11938470390051 39.0 35.0 40.0 30.0 41.0 46-47 35.6891355040012 38.5 35.0 40.0 28.5 41.0 48-49 35.25634617459207 38.0 35.0 40.0 27.0 41.0 50-51 34.940031589109225 38.0 34.0 40.0 26.0 41.0 52-53 34.570998044351995 38.0 34.0 40.0 24.0 41.0 54-55 34.37018480458734 38.0 34.0 40.0 24.0 41.0 56-57 34.070935806614905 38.0 33.0 40.0 23.5 41.0 58-59 33.70069816910408 37.0 33.0 40.0 23.0 41.0 60-61 33.28956729559052 37.0 32.5 39.5 22.0 41.0 62-63 32.96109007321924 36.0 32.0 39.0 21.5 40.0 64-65 32.52745762993165 35.5 31.5 39.0 20.0 40.0 66-67 32.11221187385379 35.0 31.0 38.5 18.5 40.0 68-69 31.760740158830707 35.0 31.0 38.0 18.0 40.0 70-71 31.393746075564913 35.0 30.5 37.5 16.5 39.5 72-73 30.936643090089817 34.5 30.0 37.0 13.0 39.0 74-75 30.44442695640869 34.0 29.5 36.0 9.0 39.0 76-77 28.969564916725908 32.5 28.0 35.0 7.5 37.0 78-79 29.717879933723726 34.0 29.0 35.0 7.0 37.0 80-81 29.509437453840345 34.0 29.0 35.0 4.5 37.0 82-83 29.21507508637215 34.0 29.0 35.0 2.0 36.5 84-85 28.83262170243723 34.0 29.0 35.0 2.0 36.0 86-87 28.441686006467194 33.5 28.5 35.0 2.0 36.0 88-89 27.975061753665805 33.0 27.0 35.0 2.0 35.0 90-91 27.508116354141023 33.0 26.5 35.0 2.0 35.0 92-93 26.895026513276 32.5 25.5 34.5 2.0 35.0 94-95 26.362093068928985 32.0 25.0 34.0 2.0 35.0 96-97 25.640975943040022 32.0 23.5 34.0 2.0 35.0 98-99 24.737993510678614 32.0 17.5 34.0 2.0 35.0 100-101 21.4720831938747 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43609275787482815 1101 2 0.3881268466785244 1101 3 0.3850208543950586 1101 4 0.2892527052244418 1101 5 0.318321471646577 1101 6 0.29870139700162923 1101 7 0.3442873002522049 1101 8 0.2899673323223553 1101 9 0.32248891850845496 1101 10-11 0.36812414435620866 1101 12-13 0.33853242647422377 1101 14-15 0.6118142913587619 1101 16-17 0.5153433691877183 1101 18-19 0.38441799732996884 1101 20-21 0.3721894929450187 1101 22-23 0.41418482973951143 1101 24-25 0.47616071775341595 1101 26-27 0.4630361831732728 1101 28-29 0.5345668076656338 1101 30-31 0.5648980607243388 1101 32-33 0.5561613803701704 1101 34-35 0.5089768507349604 1101 36-37 0.4871191905991026 1101 38-39 0.5269737944325357 1101 40-41 0.5516672032839196 1101 42-43 0.6552671683120437 1101 44-45 0.5914096646196185 1101 46-47 0.7177304092558714 1101 48-49 0.7901217650833701 1101 50-51 0.7412496396207402 1101 52-53 0.6100651689245566 1101 54-55 0.5557833681153213 1101 56-57 0.4861604789127796 1101 58-59 0.4034890854557105 1101 60-61 0.4436484266613192 1101 62-63 0.5735353660434939 1101 64-65 0.4388810896209705 1101 66-67 0.39213926512064035 1101 68-69 0.13598672798179834 1101 70-71 0.16112792956804967 1101 72-73 0.4388087521725801 1101 74-75 0.4424407007991995 1101 76-77 0.3317089809910456 1101 78-79 0.4590945826755366 1101 80-81 0.49599472895593877 1101 82-83 0.3701153499929113 1101 84-85 0.5077417708160112 1101 86-87 0.46375848920339635 1101 88-89 0.5509419008683203 1101 90-91 0.5998063025892186 1101 92-93 0.3514927906234213 1101 94-95 0.29135761046294917 1101 96-97 0.7049456594940615 1101 98-99 0.6093947940620446 1101 100-101 1.2041812370865017 1102 1 0.30088573925084816 1102 2 0.2938741146483608 1102 3 0.3329414331237004 1102 4 0.2787340042855462 1102 5 0.26718094524977687 1102 6 0.2231834656487024 1102 7 0.2522679066859439 1102 8 0.25111709375070745 1102 9 0.45129759943811365 1102 10-11 0.4149265146809711 1102 12-13 0.44334618667124204 1102 14-15 0.5539052564195543 1102 16-17 0.5582077681410809 1102 18-19 0.4509633259431709 1102 20-21 0.42550871720236927 1102 22-23 0.45329441878060805 1102 24-25 0.4261845749182669 1102 26-27 0.4404742315032735 1102 28-29 0.4341266044949421 1102 30-31 0.5894332308028325 1102 32-33 0.5661967813244644 1102 34-35 0.6531741725758167 1102 36-37 0.694360224922633 1102 38-39 0.6995403636183113 1102 40-41 0.6368926919710063 1102 42-43 0.6543921490723861 1102 44-45 0.6363442498127796 1102 46-47 0.6802615774873644 1102 48-49 0.7609706184002789 1102 50-51 0.7766013404057119 1102 52-53 0.7734798218412848 1102 54-55 0.8277927570640671 1102 56-57 0.8229120565640002 1102 58-59 0.8491655515169612 1102 60-61 0.8029134720668978 1102 62-63 0.8889578687370445 1102 64-65 0.8983900937767331 1102 66-67 0.6346111752329975 1102 68-69 0.7174112315988559 1102 70-71 0.9366343171315386 1102 72-73 1.0751162787232964 1102 74-75 0.91641484271085 1102 76-77 0.8123777511926775 1102 78-79 0.9864903037775825 1102 80-81 0.9953244195823778 1102 82-83 0.9803931705254634 1102 84-85 1.0214796498370973 1102 86-87 1.0031859172471123 1102 88-89 1.0967792103957947 1102 90-91 1.0169826916918794 1102 92-93 1.195208351651587 1102 94-95 0.8644613506507319 1102 96-97 1.1570373941408185 1102 98-99 0.9736352127168075 1102 100-101 1.6822072490920412 1103 1 0.2366979563124083 1103 2 0.2263845503000752 1103 3 0.2604765615851079 1103 4 0.19814020100493224 1103 5 0.2658078486538571 1103 6 0.2346468085847917 1103 7 0.20118318683174863 1103 8 0.08766950717888733 1103 9 0.2923073589771832 1103 10-11 0.2439861301786479 1103 12-13 0.24116057905174415 1103 14-15 0.24399770747488247 1103 16-17 0.34234160904883737 1103 18-19 0.28661636257084666 1103 20-21 0.2464245558760112 1103 22-23 0.21085864948199884 1103 24-25 0.1321771376791574 1103 26-27 0.2224812571955539 1103 28-29 0.3160763075932991 1103 30-31 0.20984610046006935 1103 32-33 0.18789632274371115 1103 34-35 0.21943762133823697 1103 36-37 0.1711326689228656 1103 38-39 0.37252642981855644 1103 40-41 0.41203996694308387 1103 42-43 0.41101775904358107 1103 44-45 0.3508752334096954 1103 46-47 0.3867519649293101 1103 48-49 0.19181620015111633 1103 50-51 0.31798325586223797 1103 52-53 0.48824788580742506 1103 54-55 0.39278908253881895 1103 56-57 0.18591032218807868 1103 58-59 0.30756874982374427 1103 60-61 0.28622510120440836 1103 62-63 0.26229036917798254 1103 64-65 0.35404239549934857 1103 66-67 0.334943793258752 1103 68-69 0.2588154881689029 1103 70-71 0.18192107708215488 1103 72-73 0.18531499704888788 1103 74-75 0.06211447231870437 1103 76-77 0.15126098947829192 1103 78-79 0.10149162660667699 1103 80-81 0.14319598757579755 1103 82-83 0.28984698909210493 1103 84-85 0.5920409703144927 1103 86-87 0.649246385234644 1103 88-89 0.5559950667071618 1103 90-91 0.5481329650765581 1103 92-93 0.5349643631748968 1103 94-95 0.20616809413946768 1103 96-97 0.38001317167036675 1103 98-99 0.40645078094539144 1103 100-101 0.8712280616030661 1104 1 0.20893712895127337 1104 2 0.20630791181629604 1104 3 0.16738176581502273 1104 4 0.19546768531449032 1104 5 0.18689038136250957 1104 6 0.1002740757030196 1104 7 0.11707634948568568 1104 8 0.12648596128732947 1104 9 0.306059514505165 1104 10-11 0.18538825734293596 1104 12-13 0.17335693493816962 1104 14-15 0.28513481487497927 1104 16-17 0.4414632667091851 1104 18-19 0.22722393895507054 1104 20-21 0.28660763732615635 1104 22-23 0.27523011673266495 1104 24-25 0.2607775474272245 1104 26-27 0.2825155365881358 1104 28-29 0.1777989049847477 1104 30-31 0.20980386567193676 1104 32-33 0.23261025056206108 1104 34-35 0.20684769937795267 1104 36-37 0.2678818332673245 1104 38-39 0.09312141204322444 1104 40-41 0.05089891009590275 1104 42-43 -0.03914547346867181 1104 44-45 -0.00967665520572325 1104 46-47 -0.10900054316734042 1104 48-49 0.031735476616930214 1104 50-51 0.005183939755049494 1104 52-53 0.04070208013151699 1104 54-55 0.03235160923572522 1104 56-57 -0.114301473267858 1104 58-59 -0.19083791101198244 1104 60-61 -0.15519440013693497 1104 62-63 -0.09573053972595602 1104 64-65 -0.029320092550605636 1104 66-67 -0.27943832450370465 1104 68-69 -0.13215010926548132 1104 70-71 -0.04196609981465116 1104 72-73 0.039927318419749724 1104 74-75 -0.060972390656385045 1104 76-77 -0.21004702897280225 1104 78-79 -0.1857282395076325 1104 80-81 -0.07647703323705812 1104 82-83 -0.2045311227218818 1104 84-85 0.08805917619199022 1104 86-87 0.22868917418839985 1104 88-89 0.25302741601230494 1104 90-91 0.25164390487545774 1104 92-93 0.15067864243188112 1104 94-95 0.18924310312319292 1104 96-97 0.41850276524457186 1104 98-99 0.47362641212240675 1104 100-101 1.3345515975955813 1105 1 0.08784320139937307 1105 2 0.15091809446090565 1105 3 0.14671216131404208 1105 4 0.235994278258147 1105 5 0.2864527549109184 1105 6 0.2503572219572803 1105 7 0.18086920445090726 1105 8 0.285543914226551 1105 9 0.2283848380826683 1105 10-11 0.27072319817051493 1105 12-13 0.28217819820692824 1105 14-15 0.3269946580057095 1105 16-17 0.18645218054194146 1105 18-19 0.2940779618480107 1105 20-21 0.2863054006919157 1105 22-23 0.24155325079213696 1105 24-25 0.29376568522828705 1105 26-27 0.2211231139834382 1105 28-29 0.15203576094771876 1105 30-31 0.2590583209253978 1105 32-33 0.2774801266741349 1105 34-35 0.2849910435203 1105 36-37 0.21873657676113112 1105 38-39 0.28284605936452323 1105 40-41 0.4462798585175065 1105 42-43 0.5167101014876749 1105 44-45 0.527382461781059 1105 46-47 0.4075470857786385 1105 48-49 0.43922584550914934 1105 50-51 0.4183785574208585 1105 52-53 0.5788114728867058 1105 54-55 0.3092635573524589 1105 56-57 0.10336627430011447 1105 58-59 0.23364284063016072 1105 60-61 0.4400115254194574 1105 62-63 0.3090912501357437 1105 64-65 0.3454304686866081 1105 66-67 0.181979596285629 1105 68-69 0.3504249302289928 1105 70-71 0.4526004310464664 1105 72-73 0.6441753991270467 1105 74-75 0.21884815201149976 1105 76-77 0.16140442747241934 1105 78-79 0.1953187104106071 1105 80-81 0.35226025231489544 1105 82-83 0.6683708201289278 1105 84-85 0.7265265135717769 1105 86-87 0.8030813750697483 1105 88-89 0.47212167614921796 1105 90-91 0.44415284309779324 1105 92-93 0.2753958065903035 1105 94-95 0.4892198221720001 1105 96-97 0.548987646484008 1105 98-99 0.2656070944759996 1105 100-101 1.1425461099427991 1106 1 0.10053780555682579 1106 2 0.14370115505356296 1106 3 0.025910524869992457 1106 4 0.11458671523293873 1106 5 0.2624379384580209 1106 6 0.03293683274896608 1106 7 0.026757085919278722 1106 8 0.08134444496516124 1106 9 0.21209447983410712 1106 10-11 0.04315188016963134 1106 12-13 0.07608907834845269 1106 14-15 0.09877373805878875 1106 16-17 0.014674759047032637 1106 18-19 0.08652709939776315 1106 20-21 -0.002190287056869522 1106 22-23 -0.01701264925208079 1106 24-25 0.09580239464889218 1106 26-27 0.13638737758361685 1106 28-29 -0.12222728637647862 1106 30-31 -0.0037374552798254967 1106 32-33 -0.03773438018123443 1106 34-35 0.02689064546634512 1106 36-37 -0.18898788144319667 1106 38-39 -0.03115161865405014 1106 40-41 0.09823032291379974 1106 42-43 0.04390492678621882 1106 44-45 -9.783299040364568E-4 1106 46-47 0.18574865588036715 1106 48-49 -0.07689976262884102 1106 50-51 -0.008550898572075027 1106 52-53 -0.21610504635255978 1106 54-55 -0.2674574731650097 1106 56-57 -0.25277647227219546 1106 58-59 -0.3079244968311343 1106 60-61 -0.12869424636294013 1106 62-63 -0.19588442434456965 1106 64-65 -0.4709533216716366 1106 66-67 -0.41915983317036165 1106 68-69 -0.4767074227121846 1106 70-71 -0.33562048890046015 1106 72-73 -0.1970176570427924 1106 74-75 -0.16953585948783712 1106 76-77 -0.2907776822135588 1106 78-79 -0.2485191931718802 1106 80-81 -0.026054652594435623 1106 82-83 0.008477520703891628 1106 84-85 0.16286994568146085 1106 86-87 0.23327210925816289 1106 88-89 0.1353254576618248 1106 90-91 0.2746664432747039 1106 92-93 0.39401533334837424 1106 94-95 0.378990388293186 1106 96-97 0.5908275403362886 1106 98-99 0.32331994277321385 1106 100-101 0.6344191749582784 1107 1 0.03330254384288622 1107 2 0.0772230599594863 1107 3 0.06994041188906408 1107 4 0.12298656617588222 1107 5 -0.04202918258062738 1107 6 0.08755787139578786 1107 7 0.03455476797410739 1107 8 0.12271479773352212 1107 9 0.1154339518697114 1107 10-11 0.15185295057208492 1107 12-13 0.108050204701307 1107 14-15 0.11061054218917832 1107 16-17 0.12039954896300031 1107 18-19 0.08842784684326688 1107 20-21 -0.001266646392373616 1107 22-23 -0.058630080286192765 1107 24-25 0.09736533344909759 1107 26-27 -0.01038818354057014 1107 28-29 0.02782523463192632 1107 30-31 0.04673928425918206 1107 32-33 0.05632602032858358 1107 34-35 0.11776198114714731 1107 36-37 0.07132891879874848 1107 38-39 -0.06115407593516409 1107 40-41 0.047708623231351055 1107 42-43 0.05020015696054969 1107 44-45 -0.005837670812638862 1107 46-47 -0.03148755094169786 1107 48-49 0.05161117902288481 1107 50-51 0.06493179784840208 1107 52-53 0.1392938162773234 1107 54-55 0.04217385365877391 1107 56-57 -0.0954525594165645 1107 58-59 -0.03473626909789118 1107 60-61 0.18567855288529245 1107 62-63 0.10397353940648202 1107 64-65 0.18954831471555877 1107 66-67 0.3894183731764471 1107 68-69 0.4707077065786045 1107 70-71 0.47120346175949734 1107 72-73 0.6182215363209309 1107 74-75 0.5566705035200883 1107 76-77 0.29913409869417507 1107 78-79 0.34075166941480006 1107 80-81 0.4624117879663636 1107 82-83 0.34386466399308446 1107 84-85 0.5760001977822995 1107 86-87 0.5047191778028015 1107 88-89 0.5388054807764711 1107 90-91 0.553587966897009 1107 92-93 0.7423393866200705 1107 94-95 0.6203306326171365 1107 96-97 0.8135560404767013 1107 98-99 0.6415501333070104 1107 100-101 1.2179553915014516 1108 1 0.04571083184888636 1108 2 0.07134163184513653 1108 3 -0.005617859015750071 1108 4 0.08011444993196903 1108 5 0.22181643234484483 1108 6 0.04902449098822359 1108 7 0.004831255501279941 1108 8 0.13541628829477048 1108 9 0.0820069297926338 1108 10-11 0.04200115163787643 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2316 70-71 -1.68473150498831 2316 72-73 -0.9264774498094539 2316 74-75 -0.9532687553010284 2316 76-77 -0.9584744733193844 2316 78-79 -1.1876738373304256 2316 80-81 -1.0198822721378278 2316 82-83 -1.415028496747336 2316 84-85 -1.4071856429256648 2316 86-87 -1.6335037825144774 2316 88-89 -1.8112411557091868 2316 90-91 -1.8480401393583499 2316 92-93 -1.6958539993404536 2316 94-95 -1.7490909991290415 2316 96-97 -2.018334974227063 2316 98-99 -2.5350535172302564 2316 100-101 -3.2030135857566258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 37.0 5 68.0 6 132.0 7 353.0 8 769.0 9 1512.0 10 2570.0 11 3939.0 12 5782.0 13 7856.0 14 10264.0 15 13735.0 16 17274.0 17 22363.0 18 19713.0 19 12824.0 20 11100.0 21 11629.0 22 12421.0 23 13956.0 24 16433.0 25 19506.0 26 23959.0 27 29620.0 28 36542.0 29 45037.0 30 55850.0 31 68605.0 32 85258.0 33 107797.0 34 141036.0 35 190119.0 36 266112.0 37 340358.0 38 201981.0 39 11060.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58401570629059 19.308821397386193 14.863759660110205 22.243403236213005 2 15.93460281206359 20.904127355097724 39.1168093059696 24.04446052686908 3 19.585154529661065 25.050901284704164 30.194254794126437 25.16968939150834 4 12.922656472586352 17.09579355880913 36.68602029780066 33.295529670803866 5 13.277739942208397 38.00237783183562 33.884214305828564 14.835667920127424 6 31.5840195620123 36.32033901586924 17.050816420804555 15.044825001313908 7 28.35054968399809 33.56286623470887 20.24486828827872 17.84171579301432 8 27.197669819123306 34.121770207210176 19.27571870755732 19.4048412661092 9 26.9476124220991 16.303189061648553 20.222064245941407 36.52713427031094 10-11 25.33410600331381 26.717000860263834 26.544007612366777 21.40488552405558 12-13 26.627099778044805 24.083687952679064 27.56252586342342 21.726686405852714 14-15 23.198304918309397 25.20949942810356 26.484738594227373 25.107457059359668 16-17 21.757153546242012 28.780788705356464 27.22293449953183 22.239123248869692 18-19 21.633192406550716 26.538512180522005 30.411195657345434 21.41709975558184 20-21 22.50870636788865 25.624963694653363 30.54077678228133 21.325553155176657 22-23 22.18634557482162 25.66427811731968 30.444281243041466 21.705095064817232 24-25 22.083332180782648 25.881217204170206 30.65883485423922 21.37661576080793 26-27 21.88199581257439 26.07755395480468 30.518277595502653 21.522172637118274 28-29 21.57558147737008 26.105853320789507 30.90132138922214 21.417243812618274 30-31 21.6352176901703 25.982437626465547 30.703504591140252 21.6788400922239 32-33 21.424635297281057 26.155355308169444 30.557459731519486 21.862549663030013 34-35 22.19578988133108 25.951453625072613 30.290614367514042 21.562142126082264 36-37 22.25260993308403 25.686925314739632 30.23126962757366 21.829195124602677 38-39 21.971687768801885 25.840692248879428 30.49689138605787 21.69072859626081 40-41 22.006685725271183 25.901113780422193 30.49025424842296 21.60194624588366 42-43 21.94588804883669 26.115225310630443 29.94893204318577 21.9899545973471 44-45 22.01797734782254 26.236831295356566 29.70187587912384 22.04331547769705 46-47 22.41541176600759 26.553900795538787 28.954956350479648 22.075731087973978 48-49 22.627159972017765 26.710751501112956 28.520247148735972 22.141841378133307 50-51 22.5495191371466 26.94602297873847 28.388805167560676 22.11565271655425 52-53 22.719273593120533 27.04300551786862 28.303805857770215 21.93391503124063 54-55 22.258701986725658 26.90986735061384 28.372816861746884 22.458613800913614 56-57 22.50466297907234 27.279575681353784 27.958232101546987 22.25752923802689 58-59 22.678553147454895 26.733154593422036 28.08883871628221 22.499453542840858 60-61 22.430382828732768 26.854092369155687 28.605553880163015 22.109970921948534 62-63 22.626316615057487 26.828391107673266 28.557928019372188 21.987364257897063 64-65 22.381541149868458 26.87344220459745 28.46573078362798 22.279285861906114 66-67 22.23885939502039 27.135674499815217 28.148740935153572 22.476725170010823 68-69 22.32829479397035 27.052876310289935 28.37277169455636 22.24605720118336 70-71 22.35410490140702 26.97220542569829 28.25164874863284 22.422040924261847 72-73 22.40353842281276 27.018701748465496 28.013592721780718 22.56416710694103 74-75 22.360521800371167 27.493769880160958 27.711274297052448 22.43443402241543 76-77 22.506906491894917 27.08904955551431 28.004382755885977 22.399661196704802 78-79 22.465430553346287 27.023272219263728 27.94356573278098 22.567731494609006 80-81 22.423311169160574 27.496346701739803 27.662536498400076 22.417805630699547 82-83 22.517270667393365 27.32160099155902 27.659682778578564 22.50144556246905 84-85 22.351884850363025 27.306119399681243 27.51945170001771 22.82254404993802 86-87 22.58790748834088 27.171772667995658 27.537328834010278 22.702991009653182 88-89 22.609912757177046 27.199355122039503 27.479012548610054 22.711719572173394 90-91 22.38029847408817 27.1400209107871 27.738756854260448 22.74092376086428 92-93 22.41030628606529 27.0654560396004 27.70832612776884 22.81591154656547 94-95 22.15048634086777 26.885340433073445 27.768233182412207 23.19594004364658 96-97 22.262505151064214 26.787913977435224 27.7092245354771 23.240356336023467 98-99 22.045723882129515 26.8779056219131 27.524331253767382 23.552039242190006 100-101 21.909823456440908 26.998659856531027 27.58799638864271 23.503520298385354 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 668.0 1 500.5 2 511.5 3 679.0 4 547.0 5 476.0 6 818.5 7 1601.0 8 2382.5 9 2886.5 10 2774.5 11 2308.5 12 2294.5 13 2552.0 14 2725.0 15 2944.0 16 3123.5 17 3215.0 18 3453.0 19 3679.5 20 3959.0 21 4421.0 22 5073.5 23 5990.5 24 6968.5 25 8403.0 26 10539.5 27 13041.0 28 15364.0 29 18091.0 30 21604.0 31 24958.5 32 29369.5 33 33075.0 34 35505.5 35 38422.0 36 42896.5 37 47513.5 38 49505.0 39 50094.0 40 51152.0 41 53603.0 42 55751.5 43 57833.5 44 60636.5 45 63495.5 46 63726.5 47 65443.0 48 76216.0 49 75997.5 50 65819.5 51 61034.0 52 57588.0 53 57159.0 54 56886.0 55 53791.5 56 49397.0 57 45314.0 58 41597.5 59 36868.0 60 31239.0 61 26403.0 62 22359.5 63 17664.0 64 13242.0 65 10315.5 66 7770.0 67 6124.0 68 4569.0 69 3033.5 70 2287.0 71 1880.5 72 1581.0 73 1355.5 74 1074.0 75 893.0 76 666.5 77 408.5 78 272.5 79 189.0 80 128.5 81 91.0 82 61.0 83 31.5 84 14.0 85 7.0 86 4.5 87 3.0 88 3.5 89 2.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022737519950652388 2 0.01266883715012019 3 0.00912820144004293 4 0.0 5 0.003098056246317601 6 0.0 7 5.532243296995715E-5 8 0.0 9 0.0 10-11 0.0 12-13 7.191916286094429E-4 14-15 1.3830608242489288E-4 16-17 1.3830608242489288E-4 18-19 0.0014937056901888433 20-21 0.0 22-23 1.3830608242489288E-4 24-25 0.0 26-27 0.0016873342055836933 28-29 0.003070395029832622 30-31 0.0015213669066738216 32-33 0.008519654677373402 34-35 0.0019362851539485005 36-37 0.0010787874429141644 38-39 0.0014937056901888433 40-41 1.3830608242489288E-4 42-43 0.005062002616751079 44-45 0.0017979790715236075 46-47 2.7661216484978576E-4 48-49 5.255631132145929E-4 50-51 5.532243296995715E-5 52-53 1.6596729890987146E-4 54-55 0.0021022524528583717 56-57 0.014605122304068687 58-59 0.027107992155279006 60-61 0.02102252452858372 62-63 0.013913591891944225 64-65 0.01900325572518028 66-67 0.0029320889474077293 68-69 9.681425769742502E-4 70-71 1.106448659399143E-4 72-73 0.0 74-75 0.001963946370433479 76-77 0.0025724931331030077 78-79 0.009543119687317608 80-81 0.017371243952566544 82-83 0.01825640288008586 84-85 0.030150725968626647 86-87 0.011175131459931344 88-89 0.04046835971752366 90-91 0.04768793722010307 92-93 0.02945919555650218 94-95 0.03156144800936056 96-97 0.0508689771158756 98-99 0.052998890785218945 100-101 0.0587524238140945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1807585.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.767881591017286 #Duplication Level Percentage of deduplicated Percentage of total 1 75.87726906636611 40.03882749543766 2 13.798254604442036 14.562093302598129 3 4.485911659931503 7.101361658970881 4 1.9367606632821357 4.087950294008474 5 1.0254936556002097 2.705656389552566 6 0.6267252256812622 1.984257749931146 7 0.4121594697456229 1.5224147467307452 8 0.28157893449160293 1.1886659099022174 9 0.2053809607386489 0.9753766395575754 >10 1.1690648005281856 11.639838297894558 >50 0.11299993858732657 4.160023966347088 >100 0.0622981944495098 6.076189606881533 >500 0.0046297301872714304 1.621714816951645 >1k 0.0011574325468178576 0.9799248426588464 >5k 3.156634218594157E-4 1.355704282576976 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9914 0.5484666004641552 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9085 0.5026043035320608 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5418 0.29973694183122784 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2415 0.13360367562244652 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2382 0.13177803533443794 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2376 0.1314461007366182 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2291 0.12674369393417184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 5.532243296995715E-5 0.0 0.0 0.0 0.0 14-15 5.532243296995715E-5 0.0 0.0 0.0 0.0 16-17 5.532243296995715E-5 0.0 0.0 0.0 0.0 18-19 5.532243296995715E-5 0.0 0.0 0.0 0.0 20-21 5.532243296995715E-5 0.0 0.0 2.7661216484978576E-5 0.0 22-23 5.532243296995715E-5 0.0 0.0 1.3830608242489288E-4 0.0 24-25 5.532243296995715E-5 0.0 0.0 3.042733813347643E-4 0.0 26-27 5.532243296995715E-5 0.0 0.0 5.25563113214593E-4 0.0 28-29 2.7661216484978576E-4 0.0 0.0 8.851589275193144E-4 0.0 30-31 2.7661216484978576E-4 0.0 0.0 0.0025171707001330505 0.0 32-33 2.7661216484978576E-4 0.0 0.0 0.004785390451901294 0.0 34-35 2.7661216484978576E-4 0.0 0.0 0.007496189667429194 0.0 36-37 2.7661216484978576E-4 0.0 0.0 0.011230453892901303 0.0 38-39 2.7661216484978576E-4 0.0 0.0 0.014411493788673839 0.0 40-41 2.7661216484978576E-4 0.0 0.0 0.019362851539485002 0.0 42-43 2.7661216484978576E-4 0.0 0.0 0.026305816877214623 0.0 44-45 3.319345978197429E-4 0.0 0.0 0.033802006544643826 0.0 46-47 3.595958143047215E-4 0.0 0.0 0.0427365794692919 0.0 48-49 4.425794637596572E-4 0.0 0.0 0.04995615697187131 0.0 50-51 4.425794637596572E-4 0.0 0.0 0.05919500327785415 0.0 52-53 4.425794637596572E-4 0.0 0.0 0.0803281726723778 0.0 54-55 4.425794637596572E-4 0.0 0.0 0.10854261348705593 0.0 56-57 4.425794637596572E-4 0.0 0.0 0.1346548018488757 0.0 58-59 4.425794637596572E-4 0.0 0.0 0.17918936038969122 2.7661216484978576E-5 60-61 4.425794637596572E-4 0.0 0.0 0.231137124948481 5.532243296995715E-5 62-63 4.979018967296144E-4 0.0 0.0 0.26778823679107755 5.532243296995715E-5 64-65 4.979018967296144E-4 0.0 0.0 0.29663888558491025 5.532243296995715E-5 66-67 4.979018967296144E-4 0.0 0.0 0.375722303515464 5.532243296995715E-5 68-69 4.979018967296144E-4 0.0 0.0 0.46255086206181173 5.532243296995715E-5 70-71 4.979018967296144E-4 0.0 0.0 0.5019957567693912 5.532243296995715E-5 72-73 4.979018967296144E-4 0.0 0.0 0.5747724173413699 5.532243296995715E-5 74-75 4.979018967296144E-4 0.0 0.0 0.6816276966228421 5.532243296995715E-5 76-77 4.979018967296144E-4 0.0 0.0 0.7944024762320998 5.532243296995715E-5 78-79 4.979018967296144E-4 0.0 0.0 0.8906081871668552 5.532243296995715E-5 80-81 5.532243296995715E-4 0.0 0.0 1.0235479935936622 5.532243296995715E-5 82-83 5.532243296995715E-4 0.0 0.0 1.1752697660137699 5.532243296995715E-5 84-85 5.532243296995715E-4 0.0 0.0 1.3394114246356326 5.532243296995715E-5 86-87 5.532243296995715E-4 0.0 0.0 1.586868667310251 5.532243296995715E-5 88-89 5.532243296995715E-4 0.0 0.0 1.8882929433470625 5.532243296995715E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11630 0.0 25.28324 1 GTATCAA 15650 0.0 25.01121 1 GTACATG 9700 0.0 24.241266 1 GGACCGT 255 1.8189894E-11 24.20855 6 TACATGG 10065 0.0 22.932964 2 GAGTACT 9740 0.0 22.524784 12-13 ACATGGG 9955 0.0 21.94473 3 CGTATAG 130 2.9411181E-4 21.9245 1 GTACCTG 1745 0.0 21.777918 1 TACCTGG 1680 0.0 21.76803 2 CATGGGG 3770 0.0 21.53934 4 GTACTTT 10315 0.0 21.429699 14-15 ATCAACG 18575 0.0 20.93962 3 TATCAAC 18650 0.0 20.93296 2 TCAACGC 18705 0.0 20.868525 4 CAACGCA 18975 0.0 20.521528 5 AACGCAG 19200 0.0 20.305212 6 GTACCCG 165 6.3221174E-5 20.152826 1 AGTACTT 9995 0.0 20.025917 12-13 GTACACG 250 2.1722553E-7 19.001234 1 >>END_MODULE