FastQCFastQC Report
Thu 26 May 2016
SRR936471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789647
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92870.5189291519500773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69340.3874507095533365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42420.23702998412536103No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31070.17360965598243674No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17980.10046674008896726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141050.030.179311
GTACATG121450.028.5952721
TACATGG126050.027.2509672
TACGGGT700.00838289927.1494264
GGTATCA96050.027.0560461
ACATGGG124000.025.9780583
TCAACGC171200.024.5883054
ATCAACG171600.024.530993
TATCAAC173250.024.5160682
CAACGCA173650.024.2147085
GAGTACT78450.023.9818512-13
AACGCAG176200.023.8912356
CATGGGA75400.022.7475434
AGTACTT82400.022.3133212-13
ACGCAGA196650.021.4786247
GTACTTT86200.021.4689614-15
CGCAGAG198100.021.3220068
CCTATAC3201.8189894E-1120.7862783
ATGGGAG28900.020.5505285
GCAGAGT204950.020.2158269
Produced by FastQC (version 0.11.5)