FastQCFastQC Report
Thu 26 May 2016
SRR936471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789647
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108520.6063765647638891No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90910.5079772714954401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57200.3196161030639003No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33310.18612609078773634No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22350.12488496334752049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105450.029.7368681
GTATCAA151700.028.9703671
GTACATG111850.026.6335721
AGGTAAG7200.026.3930132
TACATGG115350.025.6585372
TAAGGTG9200.024.7823265
ACATGGG115750.024.6641623
GAGTACT105100.023.79625112-13
GGTAAGG9000.023.7510573
GTAAGGT9100.023.4887434
ATCAACG188150.023.3786643
TCAACGC189500.023.2609464
TATCAAC189800.023.2030452
CAACGCA191800.022.982015
CATGGGG42300.022.5706624
AACGCAG196100.022.4761876
GTACTTT112400.022.01801914-15
AGTACTT107750.021.49167412-13
TTAGGGC6250.021.2809453
CATGGGA70800.021.267414
Produced by FastQC (version 0.11.5)