##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936471_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1789647 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.880947471763985 31.0 31.0 34.0 30.0 34.0 2 31.979866420584617 31.0 31.0 34.0 30.0 34.0 3 32.09491480722176 33.0 31.0 34.0 30.0 34.0 4 35.85895430774896 37.0 35.0 37.0 35.0 37.0 5 35.72094217462997 37.0 35.0 37.0 33.0 37.0 6 35.77833840975343 37.0 35.0 37.0 33.0 37.0 7 35.69847405661564 37.0 35.0 37.0 33.0 37.0 8 35.664941745495064 37.0 35.0 37.0 33.0 37.0 9 37.25698587486806 39.0 37.0 39.0 34.0 39.0 10-11 37.15550664460645 39.0 37.0 39.0 33.0 39.0 12-13 37.02290256123135 39.0 37.0 39.0 33.0 39.0 14-15 38.07583925768601 40.0 37.0 41.0 33.0 41.0 16-17 38.05550256558975 40.0 37.0 41.0 33.0 41.0 18-19 38.22218823041639 40.0 37.5 41.0 33.5 41.0 20-21 38.2388484991733 40.0 38.0 41.0 33.5 41.0 22-23 38.14221268216581 40.0 37.0 41.0 33.0 41.0 24-25 38.00444696635705 40.0 37.0 41.0 33.0 41.0 26-27 37.786447550829855 40.0 37.0 41.0 33.0 41.0 28-29 37.776501734699636 40.0 37.0 41.0 33.0 41.0 30-31 37.605644576835545 39.5 37.0 41.0 32.5 41.0 32-33 37.3687123773571 39.0 36.0 41.0 32.0 41.0 34-35 37.15636212057461 39.0 36.0 41.0 31.5 41.0 36-37 36.94113587763397 39.0 35.0 41.0 31.0 41.0 38-39 36.7046819847713 39.0 35.0 41.0 31.0 41.0 40-41 36.42949224064857 39.0 35.0 40.0 30.0 41.0 42-43 36.10550879586869 38.5 35.0 40.0 30.0 41.0 44-45 35.74082738104218 38.0 35.0 40.0 29.0 41.0 46-47 35.25245788694642 38.0 34.5 40.0 26.5 41.0 48-49 34.758455159034156 38.0 34.0 40.0 25.5 41.0 50-51 34.40152834609283 38.0 33.5 40.0 24.0 41.0 52-53 34.09485250443244 38.0 33.0 40.0 23.5 41.0 54-55 33.728942914440665 37.0 33.0 40.0 23.0 41.0 56-57 33.36110808444347 37.0 33.0 40.0 21.5 41.0 58-59 32.97125634273128 36.0 32.0 39.0 20.5 40.5 60-61 32.56415930068891 36.0 31.5 39.0 19.5 40.0 62-63 32.218020369380106 35.0 31.0 39.0 18.0 40.0 64-65 31.883648842481225 35.0 31.0 38.5 17.5 40.0 66-67 31.42241374975065 35.0 30.5 38.0 13.5 40.0 68-69 30.969317692259985 34.5 30.0 37.0 10.0 39.5 70-71 30.46310780841138 34.0 29.5 36.5 7.5 39.0 72-73 29.911942690374136 34.0 29.0 36.0 7.0 39.0 74-75 29.375446107528468 34.0 28.5 35.5 4.0 38.0 76-77 28.03851681365096 31.5 26.5 34.5 2.0 36.0 78-79 28.82074425850461 33.0 28.5 35.0 2.0 37.0 80-81 28.684829745754328 33.5 28.5 35.0 2.0 36.5 82-83 28.29920509463598 33.0 27.5 35.0 2.0 36.0 84-85 27.867258459349806 33.0 27.0 35.0 2.0 35.5 86-87 27.44019658625416 33.0 26.0 35.0 2.0 35.0 88-89 26.93910056005458 32.5 25.5 34.5 2.0 35.0 90-91 26.357319069067813 32.0 24.5 34.0 2.0 35.0 92-93 25.76300996788752 32.0 23.5 34.0 2.0 35.0 94-95 25.004615714719158 31.0 19.5 34.0 2.0 35.0 96-97 24.14835802814745 31.0 12.5 34.0 2.0 35.0 98-99 22.977118392621563 31.0 2.0 34.0 2.0 35.0 100-101 19.211635031936467 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35206456430007904 1101 2 0.47037285208820023 1101 3 0.2929095154328749 1101 4 0.26087698720461816 1101 5 0.3177097485894578 1101 6 0.29798774534704364 1101 7 0.30292123265586923 1101 8 0.22330574844961149 1101 9 0.37941110798103495 1101 10-11 0.34578966643312015 1101 12-13 0.4017729162359984 1101 14-15 0.4677304770001385 1101 16-17 0.4312332624823938 1101 18-19 0.4104704698395949 1101 20-21 0.38730297406710434 1101 22-23 0.36913789059817503 1101 24-25 0.4534568307644946 1101 26-27 0.31362568629958076 1101 28-29 0.44812374887806783 1101 30-31 0.5718803991747947 1101 32-33 0.5328953007272759 1101 34-35 0.6175483698251298 1101 36-37 0.7301782033951554 1101 38-39 0.663242532614106 1101 40-41 0.7366723208440433 1101 42-43 0.7925506065951069 1101 44-45 0.8486597054133043 1101 46-47 0.8612597564873994 1101 48-49 0.815080974788799 1101 50-51 0.7466819236811446 1101 52-53 0.5452135566695233 1101 54-55 0.5049474230182938 1101 56-57 0.6204473052178514 1101 58-59 0.6441919626805443 1101 60-61 0.6794565876622229 1101 62-63 0.5393816276631682 1101 64-65 0.6555666608776782 1101 66-67 0.6030081838179093 1101 68-69 0.4886829184954422 1101 70-71 0.44297044233740834 1101 72-73 0.6755245886720722 1101 74-75 0.693209607540723 1101 76-77 0.9150256456795454 1101 78-79 0.8357101048740461 1101 80-81 0.8939032452988371 1101 82-83 0.8390268529921308 1101 84-85 0.7722663291286018 1101 86-87 0.7922690922648314 1101 88-89 0.6096434291914186 1101 90-91 0.6284436992142801 1101 92-93 0.5917114471702902 1101 94-95 0.6796457536883516 1101 96-97 0.8304093115941029 1101 98-99 0.4205259064938609 1101 100-101 0.5458007071193727 1102 1 0.20675958504696723 1102 2 0.362725549183633 1102 3 0.2547974822378549 1102 4 0.16323175898885012 1102 5 0.10732800585087432 1102 6 0.08259770385326703 1102 7 0.29423866004176347 1102 8 0.06813769865708252 1102 9 0.20904181337522942 1102 10-11 0.27426788220074627 1102 12-13 0.32073660918205604 1102 14-15 0.5030001865437086 1102 16-17 0.4218183247230556 1102 18-19 0.3283252416238298 1102 20-21 0.37699177074760115 1102 22-23 0.27772191549444614 1102 24-25 0.25109272288067075 1102 26-27 0.3057387568389984 1102 28-29 0.31860818871209773 1102 30-31 0.396765253946576 1102 32-33 0.2963071264534278 1102 34-35 0.30802036152637413 1102 36-37 0.4468192822333279 1102 38-39 0.39053817576763095 1102 40-41 0.3204627772755728 1102 42-43 0.3238732207029855 1102 44-45 0.5016669668240965 1102 46-47 0.5008468934168633 1102 48-49 0.6151950826726207 1102 50-51 0.6223644962952548 1102 52-53 0.659467706047117 1102 54-55 0.6732586263377982 1102 56-57 0.5718290479564416 1102 58-59 0.542384908738633 1102 60-61 0.4871267121435494 1102 62-63 0.3570963579536297 1102 64-65 0.37989653639635534 1102 66-67 0.3679874369299476 1102 68-69 0.36318499318423747 1102 70-71 0.04838641744114369 1102 72-73 -0.05318806692958589 1102 74-75 0.3853164540137506 1102 76-77 0.44209099837663857 1102 78-79 0.3771032584010179 1102 80-81 0.3452694278714503 1102 82-83 0.3909096330751147 1102 84-85 0.33397172331946834 1102 86-87 0.3777846524308046 1102 88-89 0.2857544250420432 1102 90-91 0.3136075996292149 1102 92-93 0.26174982891303245 1102 94-95 0.4360326831489303 1102 96-97 0.4890358676106956 1102 98-99 0.40153005587145074 1102 100-101 0.2846523668704073 1103 1 0.3299807005848123 1103 2 0.34000841770953016 1103 3 0.2509528607239062 1103 4 0.16565207771395052 1103 5 0.22155908154807946 1103 6 0.24454591102458068 1103 7 0.25297371980271777 1103 8 0.2838767424817874 1103 9 0.29539241098478897 1103 10-11 0.35555971487007554 1103 12-13 0.33588501555656336 1103 14-15 0.33666552518912596 1103 16-17 0.40781035659556863 1103 18-19 0.334667871105907 1103 20-21 0.27797782652449143 1103 22-23 0.28058546131118334 1103 24-25 0.17665945594840338 1103 26-27 0.22673776082306318 1103 28-29 0.29588209309456914 1103 30-31 0.4132204332294478 1103 32-33 0.401019277847837 1103 34-35 0.32767673602836567 1103 36-37 0.330572270281138 1103 38-39 0.24532602437321316 1103 40-41 0.42861616372976385 1103 42-43 0.6808005115396369 1103 44-45 0.6078532218041701 1103 46-47 0.5540022719029167 1103 48-49 0.4352548436287975 1103 50-51 0.612366488327126 1103 52-53 0.5258511721825698 1103 54-55 0.4247805386883883 1103 56-57 0.44070155791660426 1103 58-59 0.3332335143163263 1103 60-61 0.26881595517144063 1103 62-63 0.4997786288699615 1103 64-65 0.44494235312941655 1103 66-67 0.3661547676072381 1103 68-69 0.3627168656941997 1103 70-71 0.0972599234172371 1103 72-73 0.31127308844889967 1103 74-75 0.4595684460456262 1103 76-77 0.44074538084676007 1103 78-79 0.31885325840101686 1103 80-81 0.513360065321649 1103 82-83 0.619828955784282 1103 84-85 0.7353920420445696 1103 86-87 0.6658752898810008 1103 88-89 0.4395582099026001 1103 90-91 0.4226434562029233 1103 92-93 0.4818324982357396 1103 94-95 0.4266432409178549 1103 96-97 0.553850608646556 1103 98-99 0.7241216893375899 1103 100-101 0.4013525660735944 1104 1 0.2652952811204017 1104 2 0.23052412134069655 1104 3 0.23606214312515306 1104 4 0.232932166943975 1104 5 0.1084967972838129 1104 6 0.11635038105877271 1104 7 0.19904105677812112 1104 8 0.17127665837151795 1104 9 0.28226414891832263 1104 10-11 0.26453904487285484 1104 12-13 0.2889096076522222 1104 14-15 0.3943502630352995 1104 16-17 0.33114341396323965 1104 18-19 0.3029744002163852 1104 20-21 0.3152872832412257 1104 22-23 0.19936584716196393 1104 24-25 0.15500131543548434 1104 26-27 0.18033883333058043 1104 28-29 0.4254877144642606 1104 30-31 0.4122141320699839 1104 32-33 0.4396193396099761 1104 34-35 0.5141441504095994 1104 36-37 0.40589857851073674 1104 38-39 0.5975023522082239 1104 40-41 0.5486175452510977 1104 42-43 0.2874319917381811 1104 44-45 0.49716301475881863 1104 46-47 0.48381451197882086 1104 48-49 0.5503034457526823 1104 50-51 0.5282138078709764 1104 52-53 0.5178485066590426 1104 54-55 0.2777216554045978 1104 56-57 0.34189891027159547 1104 58-59 0.515808162180754 1104 60-61 0.44571620729907835 1104 62-63 0.2876971993610695 1104 64-65 0.4190112737752365 1104 66-67 0.46596168476268573 1104 68-69 0.4350335398441061 1104 70-71 0.2504910834278853 1104 72-73 0.47586713449825524 1104 74-75 0.6309932770632152 1104 76-77 0.5933134614057032 1104 78-79 0.5824305149028 1104 80-81 0.7399013758571726 1104 82-83 0.6366943092607364 1104 84-85 0.6907075723760734 1104 86-87 0.8457886044960787 1104 88-89 0.6639299732317419 1104 90-91 0.5314291192620573 1104 92-93 0.620169385261832 1104 94-95 0.6134170533682095 1104 96-97 0.7226684530263014 1104 98-99 0.45116697581025633 1104 100-101 0.30268997523348773 1105 1 0.10824659803397907 1105 2 0.24247879593687927 1105 3 0.15220007964045124 1105 4 0.20858890184599232 1105 5 0.1136137201365841 1105 6 0.19263666489222686 1105 7 0.20472567302877565 1105 8 0.16078055579993844 1105 9 0.19700285233626857 1105 10-11 0.18393996012282798 1105 12-13 0.06759699879244607 1105 14-15 0.2410196670631919 1105 16-17 0.20675988316461513 1105 18-19 0.28389667019526144 1105 20-21 0.12280345905929124 1105 22-23 0.10835503237756683 1105 24-25 0.20428428132223075 1105 26-27 0.2854497958000337 1105 28-29 0.28715039650430896 1105 30-31 0.36409317602450386 1105 32-33 0.3594792043754964 1105 34-35 0.08703010178611947 1105 36-37 0.23248811340216236 1105 38-39 0.05053492901438261 1105 40-41 0.341832907145708 1105 42-43 0.5240517921315657 1105 44-45 0.31084553825267136 1105 46-47 0.18528195835192207 1105 48-49 0.16763014760768158 1105 50-51 0.15104631447707106 1105 52-53 0.13655536838477644 1105 54-55 -0.06798812690895772 1105 56-57 0.12168619081359111 1105 58-59 0.18798231133603593 1105 60-61 0.11697086798770329 1105 62-63 0.13820350081076782 1105 64-65 0.038701731201545186 1105 66-67 0.1305523719948809 1105 68-69 0.3046784996777454 1105 70-71 0.049013040817765585 1105 72-73 0.1979580369665186 1105 74-75 0.3452287916760888 1105 76-77 0.19232801136365296 1105 78-79 0.13748962203738202 1105 80-81 0.4254025447545686 1105 82-83 0.42595833437381714 1105 84-85 0.44829639864414617 1105 86-87 0.4147034835996344 1105 88-89 0.27670572374334057 1105 90-91 0.4457634437850615 1105 92-93 0.2309413873545907 1105 94-95 0.4219774883437353 1105 96-97 0.5453930104678406 1105 98-99 0.29589369223508655 1105 100-101 0.08666210713014877 1106 1 0.021757702314314287 1106 2 0.18096492519223872 1106 3 0.08478134452940367 1106 4 0.02954025818196726 1106 5 0.08406173366152103 1106 6 0.06161384156171579 1106 7 0.059452484678665485 1106 8 0.07413796761888847 1106 9 0.11576101724828192 1106 10-11 0.029815622921574914 1106 12-13 0.13435024554569708 1106 14-15 0.20435171962601117 1106 16-17 0.17219137474995705 1106 18-19 0.10005896943447823 1106 20-21 0.02839171695524101 1106 22-23 -0.060211785420023034 1106 24-25 -0.03396362461798219 1106 26-27 -0.06641065144502534 1106 28-29 0.06265028123495853 1106 30-31 0.07226807804556046 1106 32-33 0.022138083957457866 1106 34-35 0.20942770418372447 1106 36-37 0.2182208422118137 1106 38-39 0.15483524408392668 1106 40-41 0.06130113122931391 1106 42-43 0.07507615238669274 1106 44-45 0.2732308452533587 1106 46-47 0.24107846953305057 1106 48-49 0.24870906868660825 1106 50-51 0.3014067232990172 1106 52-53 0.2930127571498602 1106 54-55 0.3273134945465088 1106 56-57 0.3640469070204517 1106 58-59 0.4146694703308853 1106 60-61 0.4352095523802362 1106 62-63 0.4112688969530822 1106 64-65 0.45589223300742887 1106 66-67 0.2920985181564113 1106 68-69 0.2983678872132849 1106 70-71 0.1990414739231241 1106 72-73 0.35021039998811077 1106 74-75 0.36677032706377943 1106 76-77 0.24130979880697723 1106 78-79 0.371637013107847 1106 80-81 0.4378147479360024 1106 82-83 0.5608618923542998 1106 84-85 0.5709383720553483 1106 86-87 0.438467411978948 1106 88-89 0.30877365581127236 1106 90-91 0.1851041031257239 1106 92-93 0.16723893595982986 1106 94-95 0.1813873092920204 1106 96-97 0.18995921349558387 1106 98-99 0.445490249523953 1106 100-101 0.2392587412652425 1107 1 0.15303018278525116 1107 2 0.2522037398136838 1107 3 0.15658531207091642 1107 4 0.14034672937118842 1107 5 0.11939262620628455 1107 6 0.1427447151618324 1107 7 0.07748987167342847 1107 8 0.10238837178578564 1107 9 0.11129975629391708 1107 10-11 0.17296457040753666 1107 12-13 0.17757734693111615 1107 14-15 0.3010279194462342 1107 16-17 0.260994684443034 1107 18-19 0.1892401581558687 1107 20-21 0.18913905130764874 1107 22-23 0.11352899680839101 1107 24-25 0.24439953494314182 1107 26-27 0.21383393723748867 1107 28-29 0.30855581929906606 1107 30-31 0.14703410155023988 1107 32-33 0.1630972449240744 1107 34-35 0.1960649226464639 1107 36-37 0.2948357065736644 1107 38-39 0.3654841908457058 1107 40-41 0.5006865252561923 1107 42-43 0.36065768489254424 1107 44-45 0.3723087277287789 1107 46-47 0.3560065595450226 1107 48-49 0.3981285775568466 1107 50-51 0.26687634336041555 1107 52-53 0.24843418423774466 1107 54-55 0.3642247006511994 1107 56-57 0.3812307711659244 1107 58-59 0.2677866319481055 1107 60-61 0.3654670460153895 1107 62-63 0.13325696646197827 1107 64-65 0.20690138292301086 1107 66-67 0.27453967171723903 1107 68-69 0.33066749183798194 1107 70-71 0.30742424727420925 1107 72-73 0.33619440479900575 1107 74-75 0.47955352994266676 1107 76-77 0.1621627538962933 1107 78-79 0.41437022124431167 1107 80-81 0.570651899600044 1107 82-83 0.76181633743699 1107 84-85 0.6424709155650277 1107 86-87 0.6295012652471748 1107 88-89 0.6288929549289577 1107 90-91 0.47643797119626186 1107 92-93 0.739845009311523 1107 94-95 0.8334224246675106 1107 96-97 1.0150075962052085 1107 98-99 1.2418133084293395 1107 100-101 1.0129936431224245 1108 1 -0.05551137724903654 1108 2 0.12576494141773864 1108 3 -0.0604416848977678 1108 4 -0.019715624241314345 1108 5 0.10037471378559104 1108 6 0.03407378713970388 1108 7 0.01010669605751957 1108 8 0.0707057909474571 1108 9 -0.005042035808791923 1108 10-11 0.0207730876031178 1108 12-13 0.05285436382471431 1108 14-15 0.01618955064049743 1108 16-17 0.007429185724745935 1108 18-19 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68-69 -1.55994531843049 2316 70-71 -1.2315307213690545 2316 72-73 -1.1258814097057908 2316 74-75 -1.4241049340882306 2316 76-77 -1.7039451206035316 2316 78-79 -1.6642203959899184 2316 80-81 -1.8693275976242987 2316 82-83 -1.880156230947545 2316 84-85 -1.5640254438193395 2316 86-87 -1.9503463673992272 2316 88-89 -2.174970079207249 2316 90-91 -2.3770326269996787 2316 92-93 -2.334752295732862 2316 94-95 -2.450072840930389 2316 96-97 -2.2514584666669215 2316 98-99 -1.8702149866214661 2316 100-101 -1.2988100977188282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 34.0 5 90.0 6 179.0 7 388.0 8 904.0 9 1775.0 10 2914.0 11 4440.0 12 6466.0 13 8937.0 14 11419.0 15 15478.0 16 19754.0 17 25134.0 18 21640.0 19 14353.0 20 13164.0 21 13594.0 22 14342.0 23 16261.0 24 18430.0 25 22143.0 26 26968.0 27 33140.0 28 40448.0 29 49209.0 30 60454.0 31 74099.0 32 92453.0 33 116633.0 34 153741.0 35 204957.0 36 271952.0 37 301455.0 38 128503.0 39 3783.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9540253780271 19.038549878117067 14.937952031438083 22.069472712417753 2 16.373282726000635 21.040713867392693 38.2099891364118 24.376014270194872 3 19.454353836834777 25.299069932742796 30.07491277126962 25.171663459152814 4 13.28357283228629 17.067464443946516 36.08247249864358 33.566490225123616 5 13.8489581499779 37.830703352840956 32.750284008835706 15.57005448834544 6 31.90886247399627 36.04163279127113 16.723465577289822 15.32603915744278 7 28.8580857421923 33.744141292905184 19.6227853137272 17.774987651175316 8 28.098875229170172 32.63489399081028 19.107413965843936 20.15881681417561 9 27.841371980827496 15.968744656764521 19.75480067007665 36.435082692331335 10-11 25.883568100301346 26.195808447140696 25.968780435471352 21.95184301708661 12-13 27.269344177930062 23.83693544034103 26.917738526089224 21.975981855639688 14-15 23.771140117001412 24.81179837358033 26.1023397768206 25.314721732597654 16-17 22.48554188153493 28.13263890033642 26.628251382807726 22.753567835320922 18-19 22.452404673843986 25.591089500055595 30.106100795755967 21.850405030344447 20-21 23.473015213132488 24.509621498872956 30.118130035236174 21.89923325275838 22-23 22.828785159005665 24.68522256602245 30.194829958581 22.29116231639089 24-25 22.93054632805834 24.97461778604512 30.267528814470406 21.827307071426137 26-27 22.51692652238123 25.28339387599901 30.11554792649053 22.08413167512923 28-29 22.0691287164452 25.4432857429426 30.37830924199018 22.109276298622017 30-31 22.19604378755292 25.104113059546577 30.25933692846778 22.44050622443272 32-33 22.158250185517975 25.220107019284928 30.088975985480428 22.532666809716673 34-35 22.988628099487578 25.04282308554755 29.753488220054365 22.21506059491051 36-37 23.20936846928398 24.68487723650831 29.774924285602527 22.33083000860518 38-39 22.68263489115165 24.955519643441885 29.793205457896217 22.56864000751025 40-41 22.561354993849346 25.15709107916005 29.82236994847865 22.459183978511955 42-43 22.77155081612343 25.321059957454633 29.113906367779958 22.79348285864198 44-45 22.639748698074193 25.41372588409277 29.02542272250645 22.92110269532659 46-47 23.243410572029006 25.870464957614548 28.25188431014608 22.634240160210364 48-49 23.42041657691245 25.96633511388937 27.716353763209234 22.896894545988943 50-51 23.16898110896542 26.20016293852591 27.635569770768516 22.995286181740155 52-53 23.654364737449697 26.23985968111987 27.29683321431485 22.808942367115584 54-55 22.861175579185932 26.191779321658952 27.501815303645333 23.445229795509782 56-57 23.267719021574823 26.368202019953653 27.134891393821665 23.229187564649862 58-59 23.731335652462533 25.722916492629533 27.169322228856203 23.37642562605173 60-61 23.262715629119313 25.94341494052545 27.977401515096485 22.816467915258755 62-63 23.22168543017494 26.035848470422053 27.793628229971993 22.948837869431017 64-65 23.010248756274507 26.11759275764242 27.655688503969923 23.216469982113153 66-67 23.093458528811574 26.35285098355636 27.25996687579765 23.293723611834416 68-69 23.20070786234011 26.036124559856084 27.649126473397743 23.114041104406063 70-71 23.172075547188495 26.188688038436332 27.38781162041779 23.251424793957387 72-73 23.257498755306365 26.18279422685666 27.291200197586384 23.268506820250593 74-75 23.06843347290529 26.74791382488814 26.93072831302713 23.252924389179437 76-77 23.191833366068337 26.396725344744297 27.177559972809924 23.23388131637744 78-79 23.158093848000483 26.417940556525394 27.221977589671493 23.201988005802633 80-81 23.150452615109522 26.959991059454623 26.88866227089852 23.000894054537326 82-83 23.24077809653341 26.740334980258133 26.760535047535818 23.258351875672638 84-85 22.956707934457302 26.707269508697973 26.658795314291588 23.677227242553133 86-87 23.148849976601408 26.5047530575326 26.59700637698975 23.74939058887624 88-89 23.00546991602067 26.62687953276893 26.467151726608133 23.900498824602273 90-91 22.862342235915683 26.536581876664396 26.784556240446 23.816519646973916 92-93 23.035349680320696 26.348816443395922 26.955430413174742 23.66040346310864 94-95 22.783005083142374 26.12071359563175 27.05542620000324 24.040855121222638 96-97 22.88858324176492 25.95882815898456 27.109307401940775 24.043281197309746 98-99 22.74683483475429 26.236032091406198 26.623428872207676 24.393704201631838 100-101 22.558446279042933 26.2076979191317 26.944812385467586 24.289043416357778 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 853.0 1 647.5 2 565.0 3 650.5 4 537.0 5 546.0 6 893.5 7 1745.5 8 2728.0 9 3282.0 10 2928.0 11 2382.0 12 2436.0 13 2525.5 14 2696.0 15 2920.0 16 3125.0 17 3406.5 18 3632.0 19 3822.0 20 4047.0 21 4441.5 22 5065.5 23 5822.5 24 6614.5 25 7774.5 26 9527.0 27 11504.0 28 13328.0 29 15208.0 30 17740.0 31 21125.0 32 25626.0 33 28279.5 34 29542.0 35 31228.0 36 33716.5 37 37236.5 38 39008.5 39 39252.0 40 40780.0 41 45273.5 42 49741.0 43 53040.0 44 55011.0 45 57393.5 46 59226.0 47 62265.0 48 72879.5 49 74594.0 50 66835.5 51 62622.5 52 61382.0 53 63045.0 54 63859.0 55 61438.0 56 56997.0 57 52373.0 58 49834.5 59 46218.0 60 40273.5 61 34978.0 62 29963.0 63 23332.0 64 17170.5 65 13845.0 66 10619.5 67 8374.0 68 6403.5 69 4401.0 70 3401.5 71 2708.0 72 2359.0 73 2236.5 74 1912.5 75 1615.5 76 1157.5 77 697.0 78 467.5 79 334.5 80 220.5 81 161.5 82 106.0 83 60.0 84 31.5 85 13.0 86 6.0 87 3.0 88 1.0 89 1.5 90 2.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01257231174639468 2 0.010225480220401007 3 0.005531817168413659 4 4.4701552876069976E-4 5 0.005028924698557873 6 0.0 7 1.6763082328526238E-4 8 0.0021233237616133235 9 5.587694109508747E-5 10-11 0.0 12-13 0.0 14-15 5.867078814984184E-4 16-17 0.004553970699249629 18-19 0.0015645543506624493 20-21 5.028924698557872E-4 22-23 5.308309404033309E-4 24-25 4.4701552876069976E-4 26-27 0.0 28-29 0.0 30-31 3.3526164657052477E-4 32-33 0.0035202472889905106 34-35 9.219695280689431E-4 36-37 0.0015086774095673617 38-39 0.005196555521843134 40-41 2.7938470547543735E-5 42-43 0.0015645543506624493 44-45 0.004833355404725066 46-47 0.0 48-49 0.00209538529106578 50-51 8.381541164263121E-4 52-53 5.028924698557872E-4 54-55 8.381541164263119E-5 56-57 0.011175388219017493 58-59 0.0021512622321608675 60-61 0.003240862583515073 62-63 1.6763082328526238E-4 64-65 0.003576124230085598 66-67 0.0010616618808066618 68-69 0.0018718775266854302 70-71 0.005028924698557873 72-73 0.0027938470547543733 74-75 0.007152248460171196 76-77 0.0013131081157345555 78-79 0.0064258482259350585 80-81 0.002626216231469111 82-83 0.002737970113659286 84-85 0.0019556929383280613 86-87 5.308309404033309E-4 88-89 0.0022350776438034986 90-91 0.0082418488115254 92-93 0.002290954584898586 94-95 3.3526164657052477E-4 96-97 8.381541164263119E-5 98-99 5.028924698557872E-4 100-101 0.0018718775266854302 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1789647.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.48821713909262 #Duplication Level Percentage of deduplicated Percentage of total 1 69.63843603203354 29.588129913558518 2 15.183749679822208 12.902609067638279 3 6.051885718806107 7.714015035648222 4 2.851058867616105 4.845456329744343 5 1.6393667292072982 3.4826884780581864 6 1.0244695183464914 2.6116730008732407 7 0.6615517001044432 1.967570659794142 8 0.4765509150484342 1.619823900512892 9 0.33072449676731025 1.2646704808679952 >10 1.8466618822922605 14.352625934990094 >50 0.1671195605574034 4.895043481100654 >100 0.11681031129433929 9.04463575344582 >500 0.00831498921088659 2.451153194127067 >1k 0.0029036470260238884 1.82078455868478 >5k 2.639679114567171E-4 0.8305657734663919 >10k+ 1.3198395572835855E-4 0.6085544374895202 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10852 0.6063765647638891 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9091 0.5079772714954401 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5720 0.3196161030639003 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3331 0.18612609078773634 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2235 0.12488496334752049 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1175388219017493E-4 2 0.0 0.0 0.0 0.0 1.1175388219017493E-4 3 0.0 0.0 0.0 0.0 1.1175388219017493E-4 4 0.0 0.0 0.0 0.0 1.1175388219017493E-4 5 0.0 0.0 0.0 0.0 1.1175388219017493E-4 6 0.0 0.0 0.0 0.0 1.1175388219017493E-4 7 0.0 0.0 0.0 0.0 1.1175388219017493E-4 8 0.0 0.0 0.0 0.0 1.1175388219017493E-4 9 0.0 0.0 0.0 0.0 1.1175388219017493E-4 10-11 0.0 0.0 0.0 0.0 1.1175388219017493E-4 12-13 0.0 0.0 0.0 0.0 1.676308232852624E-4 14-15 0.0 0.0 0.0 0.0 1.9556929383280613E-4 16-17 0.0 0.0 0.0 0.0 2.793847054754373E-4 18-19 0.0 0.0 0.0 2.7938470547543732E-5 2.793847054754373E-4 20-21 0.0 0.0 0.0 8.381541164263119E-5 3.352616465705248E-4 22-23 0.0 0.0 0.0 2.2350776438034988E-4 3.352616465705248E-4 24-25 0.0 0.0 0.0 5.587694109508747E-4 3.352616465705248E-4 26-27 0.0 0.0 0.0 8.940310575213995E-4 3.352616465705248E-4 28-29 0.0 0.0 0.0 0.0015366158801149053 3.352616465705248E-4 30-31 0.0 0.0 0.0 0.002626216231469111 3.352616465705248E-4 32-33 0.0 0.0 0.0 0.0047495399930824345 3.6320011711806854E-4 34-35 0.0 0.0 0.0 0.006984617636885933 3.9113858766561227E-4 36-37 0.0 0.0 0.0 0.01039311104368627 3.9113858766561227E-4 38-39 0.0 0.0 0.0 0.01572935891826712 3.9113858766561227E-4 40-41 0.0 0.0 0.0 0.02299336126062849 3.9113858766561227E-4 42-43 0.0 0.0 0.0 0.029754471133134078 3.9113858766561227E-4 44-45 0.0 0.0 0.0 0.03481133430223949 3.9113858766561227E-4 46-47 0.0 0.0 0.0 0.04277379840828946 3.9113858766561227E-4 48-49 0.0 0.0 0.0 0.049143769693129426 3.9113858766561227E-4 50-51 0.0 0.0 0.0 0.055876941095087465 3.9113858766561227E-4 52-53 0.0 0.0 0.0 0.0704608227209053 3.9113858766561227E-4 54-55 0.0 0.0 0.0 0.09753320068147517 3.9113858766561227E-4 56-57 0.0 0.0 0.0 0.1345516741569706 4.7495399930824343E-4 58-59 0.0 0.0 0.0 0.17933704244468324 5.028924698557872E-4 60-61 0.0 0.0 0.0 0.23283921354322948 5.308309404033309E-4 62-63 0.0 0.0 0.0 0.26820931725641983 5.587694109508747E-4 64-65 0.0 0.0 0.0 0.3082172070805025 5.587694109508747E-4 66-67 0.0 0.0 0.0 0.3845171701458444 5.587694109508747E-4 68-69 0.0 0.0 0.0 0.49129800457855655 5.587694109508747E-4 70-71 0.0 0.0 0.0 0.6023813634755903 5.587694109508747E-4 72-73 0.0 0.0 0.0 0.7320717437572885 5.867078814984184E-4 74-75 0.0 0.0 0.0 0.8500000279384705 6.425848225935059E-4 76-77 0.0 0.0 0.0 0.9542664000219038 6.984617636885933E-4 78-79 0.0 0.0 0.0 1.0555154172862022 7.543387047836809E-4 80-81 0.0 0.0 0.0 1.1612066513675603 7.822771753312245E-4 82-83 0.0 0.0 0.0 1.3016533428100625 9.49907998616487E-4 84-85 0.0 0.0 0.0 1.527396184834216 0.0010057849397115744 86-87 0.0 0.0 0.0 1.8137375694759916 0.0010337234102591182 88-89 0.0 0.0 0.0 2.1258382239626026 0.001061661880806662 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10545 0.0 29.736868 1 GTATCAA 15170 0.0 28.970367 1 GTACATG 11185 0.0 26.633572 1 AGGTAAG 720 0.0 26.393013 2 TACATGG 11535 0.0 25.658537 2 TAAGGTG 920 0.0 24.782326 5 ACATGGG 11575 0.0 24.664162 3 GAGTACT 10510 0.0 23.796251 12-13 GGTAAGG 900 0.0 23.751057 3 GTAAGGT 910 0.0 23.488743 4 ATCAACG 18815 0.0 23.378664 3 TCAACGC 18950 0.0 23.260946 4 TATCAAC 18980 0.0 23.203045 2 CAACGCA 19180 0.0 22.98201 5 CATGGGG 4230 0.0 22.570662 4 AACGCAG 19610 0.0 22.476187 6 GTACTTT 11240 0.0 22.018019 14-15 AGTACTT 10775 0.0 21.491674 12-13 TTAGGGC 625 0.0 21.280945 3 CATGGGA 7080 0.0 21.26741 4 >>END_MODULE