FastQCFastQC Report
Thu 26 May 2016
SRR936470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1854636
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95640.5156807050008735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72640.3916671519370917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43930.23686588635182323No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30490.1643988362136829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTA4250.031.3093554
GTATCAA147050.030.2517811
GTATCGA650.005828791329.247371
GGTATCA101000.028.2809031
GTACATG127950.026.6329351
TATCACG1257.3646497E-626.613672
TACATGG132450.025.3319992
ATCAACG178600.024.9589543
TCAACGC178400.024.933664
CAACGCA180750.024.5312715
TATCAAC181850.024.356752
AACGCAG184100.024.10946
ACATGGG131150.024.0605073
GAGTACT83600.023.61753712-13
AGTACTT86000.022.29538312-13
CATGGGA80800.021.9382744
GTACTTT90700.021.76846114-15
ACGCAGA204250.021.6838027
CGCAGAG207000.021.349828
GTGTAAG6600.020.8830641
Produced by FastQC (version 0.11.5)