FastQCFastQC Report
Thu 26 May 2016
SRR936470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1854636
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110540.5960199198117583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92780.5002598892720728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61740.3328955115720821No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33790.18219208513152985No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22030.11878341626065708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA157000.028.9249691
GGTATCA109250.027.9897751
GTAAGGT7650.026.0796094
GTACATG119750.025.4152951
AGGTAAG7350.025.207942
TAAGGTG8000.024.933925
GGTAAGG7900.024.6543433
TACATGG124200.024.3273932
ACATGGG123350.023.3383463
ATCAACG195500.023.2299233
TCAACGC195750.023.1747364
TATCAAC198450.022.9337182
CAACGCA198600.022.8139445
AACGCAG200700.022.622566
GAGTACT106700.021.98603612-13
AGTACTT108600.021.40460812-13
GTACTTT113250.020.67250614-15
ACGCAGA220850.020.5585147
CATGGGA75950.020.3893344
CGCAGAG224300.020.1576048
Produced by FastQC (version 0.11.5)