##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936470_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1854636 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.441967588249124 31.0 31.0 34.0 30.0 34.0 2 31.638940471337772 31.0 31.0 34.0 30.0 34.0 3 31.906645832389753 31.0 31.0 34.0 30.0 34.0 4 35.742058279899666 37.0 35.0 37.0 35.0 37.0 5 35.54806711397816 37.0 35.0 37.0 33.0 37.0 6 35.65810487880101 37.0 35.0 37.0 33.0 37.0 7 35.58535637181636 37.0 35.0 37.0 33.0 37.0 8 35.564070254217 37.0 35.0 37.0 33.0 37.0 9 37.17708596188147 39.0 37.0 39.0 33.0 39.0 10-11 37.05898704651479 39.0 37.0 39.0 33.0 39.0 12-13 36.919293058044815 39.0 37.0 39.0 33.0 39.0 14-15 38.00214085135843 40.0 37.0 41.0 33.0 41.0 16-17 37.916060078635375 40.0 37.0 41.0 33.0 41.0 18-19 37.99629954341445 40.0 37.0 41.0 33.0 41.0 20-21 38.07695445359629 40.0 37.0 41.0 33.0 41.0 22-23 37.98596436173999 40.0 37.0 41.0 33.0 41.0 24-25 37.86142941256398 40.0 37.0 41.0 33.0 41.0 26-27 37.637021226806766 39.5 37.0 41.0 32.0 41.0 28-29 37.53212598051586 39.0 36.5 41.0 32.0 41.0 30-31 37.47320956780737 39.0 36.5 41.0 32.0 41.0 32-33 37.26261487429339 39.0 36.0 41.0 31.5 41.0 34-35 37.06431720294441 39.0 35.5 41.0 31.0 41.0 36-37 36.79079938057926 39.0 35.0 41.0 31.0 41.0 38-39 36.606235671042725 39.0 35.0 41.0 30.0 41.0 40-41 36.32911444617704 39.0 35.0 40.0 30.0 41.0 42-43 36.03675572996534 38.5 35.0 40.0 30.0 41.0 44-45 35.68929320901783 38.0 35.0 40.0 28.5 41.0 46-47 35.19313655078409 38.0 34.5 40.0 26.5 41.0 48-49 34.661850896887586 38.0 34.0 40.0 24.5 41.0 50-51 34.413999296897074 38.0 33.5 40.0 24.0 41.0 52-53 34.10571103979433 38.0 33.0 40.0 23.5 41.0 54-55 33.790049098583225 37.0 33.0 40.0 23.0 41.0 56-57 33.48996191166353 37.0 33.0 40.0 22.0 41.0 58-59 33.098709665939836 36.5 32.0 39.5 20.5 41.0 60-61 32.696676328940015 36.0 32.0 39.0 20.0 40.5 62-63 32.382977306598164 35.5 31.0 39.0 18.5 40.0 64-65 31.93287820359359 35.0 31.0 38.5 18.0 40.0 66-67 31.51087976292922 35.0 31.0 38.0 15.0 40.0 68-69 31.0547986235574 35.0 30.0 37.0 12.5 39.5 70-71 30.576355414216053 34.0 29.5 37.0 8.0 39.0 72-73 30.174535057013884 34.0 29.0 36.0 7.5 39.0 74-75 29.81614802042018 34.0 29.0 36.0 7.0 38.5 76-77 28.434601183197135 32.5 27.0 34.5 3.5 36.5 78-79 29.23148639409566 34.0 29.0 35.0 2.0 37.0 80-81 29.094894362020362 34.0 29.0 35.0 2.0 36.5 82-83 28.774613994336356 34.0 29.0 35.0 2.0 36.0 84-85 28.343249025684827 33.0 28.0 35.0 2.0 36.0 86-87 27.96115194571873 33.0 27.0 35.0 2.0 35.0 88-89 27.54039471896372 33.0 26.5 35.0 2.0 35.0 90-91 27.0888834790223 33.0 25.5 35.0 2.0 35.0 92-93 26.626661781611055 32.0 25.0 34.0 2.0 35.0 94-95 26.072336296718063 32.0 24.5 34.0 2.0 35.0 96-97 25.36437096012371 32.0 21.5 34.0 2.0 35.0 98-99 24.47203629175752 31.5 10.0 34.0 2.0 35.0 100-101 21.663717840050555 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4454059052422963 1101 2 0.4386681713264302 1101 3 0.21834713756787139 1101 4 0.1996043603546127 1101 5 0.2623665471547909 1101 6 0.20751601910629347 1101 7 0.17483574082029918 1101 8 0.232385778292155 1101 9 0.1516226064488535 1101 10-11 0.2731536022016243 1101 12-13 0.25895220831450416 1101 14-15 0.43114019238198154 1101 16-17 0.33156796868207294 1101 18-19 0.37807010610559644 1101 20-21 0.23071885898325206 1101 22-23 0.14659624132087146 1101 24-25 0.16436351058793264 1101 26-27 0.2911041080223171 1101 28-29 0.3187377323961158 1101 30-31 0.1278283492680572 1101 32-33 0.3007074859487133 1101 34-35 0.2744477901452669 1101 36-37 0.22984355671185597 1101 38-39 0.3101372841217156 1101 40-41 0.2062878721144159 1101 42-43 0.37698084877325044 1101 44-45 0.42372407680486646 1101 46-47 0.47472916879109306 1101 48-49 0.455299868910636 1101 50-51 0.3182577077495736 1101 52-53 0.37463962096991565 1101 54-55 0.3773345963113357 1101 56-57 0.3249758595583998 1101 58-59 0.24441433434678572 1101 60-61 0.39351400071650744 1101 62-63 0.20128052072651315 1101 64-65 0.3021221147253321 1101 66-67 0.1176343638591888 1101 68-69 -0.08615191836375402 1101 70-71 0.1362758825809145 1101 72-73 -0.021280492812778107 1101 74-75 0.26803479519269047 1101 76-77 0.5995502577471292 1101 78-79 0.6788039478188814 1101 80-81 0.4409631361657915 1101 82-83 0.28406315369554136 1101 84-85 0.5457365973334056 1101 86-87 0.2819271574642741 1101 88-89 0.18876686597069892 1101 90-91 0.283494058363047 1101 92-93 0.17877550099368378 1101 94-95 -0.009128616635788234 1101 96-97 0.4081418478757861 1101 98-99 0.42599478843579774 1101 100-101 0.48098046359160307 1102 1 0.4631087825273852 1102 2 0.528012702586107 1102 3 0.227199608087151 1102 4 0.19817056762975227 1102 5 0.1344762604168821 1102 6 0.2383660378864505 1102 7 0.17304904625168405 1102 8 -0.04149391313542594 1102 9 0.10170144183149432 1102 10-11 0.20730645203880016 1102 12-13 0.22636063957293828 1102 14-15 0.2295517213343743 1102 16-17 0.29823422259804744 1102 18-19 0.2853193497399431 1102 20-21 0.23358979803236224 1102 22-23 0.08925072358703545 1102 24-25 0.08403990372471526 1102 26-27 -0.16524750603927885 1102 28-29 0.01075425687017173 1102 30-31 0.1595423068455375 1102 32-33 0.18805905048021998 1102 34-35 0.4011836647846181 1102 36-37 0.3725598483945163 1102 38-39 0.20852735003800404 1102 40-41 0.37382570071556387 1102 42-43 0.10955812602201576 1102 44-45 0.15121291189857544 1102 46-47 0.2023023598079945 1102 48-49 0.1792509146145207 1102 50-51 0.14166593469641242 1102 52-53 -0.020303718678505334 1102 54-55 -0.02198157897563391 1102 56-57 0.15965218046380159 1102 58-59 0.15835633049168507 1102 60-61 0.20511343029505014 1102 62-63 0.12980628875237699 1102 64-65 0.04026198148940452 1102 66-67 0.045337210187977206 1102 68-69 -0.12530209564017625 1102 70-71 -0.28248910542867733 1102 72-73 -0.09704243059474038 1102 74-75 -0.6552462013525862 1102 76-77 -0.17858644115536748 1102 78-79 -0.08363016685151337 1102 80-81 0.08216695452246725 1102 82-83 0.1251350466115042 1102 84-85 0.14717162831034614 1102 86-87 0.07674053223777832 1102 88-89 -0.05195733607902042 1102 90-91 0.07309542374234113 1102 92-93 0.5943522478578132 1102 94-95 0.23650241726342713 1102 96-97 0.1860223833785355 1102 98-99 -0.1310979194976838 1102 100-101 -0.38777565027806205 1103 1 0.47079692896378944 1103 2 0.4309693314441425 1103 3 0.2529909138666362 1103 4 0.17717369580864784 1103 5 0.12898210223216466 1103 6 0.15941921104761292 1103 7 0.10485900938385839 1103 8 0.18510354551322195 1103 9 0.0894753779860693 1103 10-11 0.09411870838483338 1103 12-13 0.14298357347545476 1103 14-15 0.19727620639027066 1103 16-17 0.15377939009085395 1103 18-19 0.13452363393382427 1103 20-21 0.0785189289043089 1103 22-23 0.18694016363125598 1103 24-25 0.12324614303039994 1103 26-27 0.13265567329592898 1103 28-29 0.19680850313781661 1103 30-31 0.23876733286469687 1103 32-33 0.0742634581548387 1103 34-35 0.1078783057202557 1103 36-37 0.10534971944533567 1103 38-39 0.3006425164136033 1103 40-41 0.3183758632985416 1103 42-43 0.2493552353965569 1103 44-45 0.19802277213013753 1103 46-47 0.1008570470782999 1103 48-49 -0.03521639826804801 1103 50-51 -0.15880314513682947 1103 52-53 -0.05582905575154484 1103 54-55 0.09669297281865852 1103 56-57 -0.0176669055434715 1103 58-59 0.0028698163531402088 1103 60-61 0.02187167851487004 1103 62-63 -0.22462497951248395 1103 64-65 -0.0498991755839846 1103 66-67 -0.12222432438369069 1103 68-69 -0.09084325000874571 1103 70-71 -0.25595177014317017 1103 72-73 -0.2876886170977073 1103 74-75 -0.20303913002187457 1103 76-77 -0.2520685099870619 1103 78-79 -0.10085720090280148 1103 80-81 -0.25399798341375757 1103 82-83 -0.04849284710097379 1103 84-85 0.1366720898931355 1103 86-87 -0.15830212741988348 1103 88-89 -0.2609363708123915 1103 90-91 -0.3823530171662419 1103 92-93 -0.32402146661942766 1103 94-95 -0.16904657225247277 1103 96-97 -0.11022960848349683 1103 98-99 -0.26150975718518055 1103 100-101 0.038220427738249185 1104 1 0.2578693543963908 1104 2 0.2584141061802825 1104 3 0.07668557504299756 1104 4 -0.008625592479660327 1104 5 -0.034062975893441205 1104 6 0.07315251423919022 1104 7 0.02913065986341934 1104 8 -0.02337671128328367 1104 9 -0.0348896556029743 1104 10-11 -0.1089571594055343 1104 12-13 -0.05343857170218058 1104 14-15 0.06455986357143217 1104 16-17 -0.06601353321597259 1104 18-19 0.02960602023143366 1104 20-21 -0.13693160938663596 1104 22-23 -0.09009146479129981 1104 24-25 -0.044344847400999754 1104 26-27 0.04623333797168527 1104 28-29 -0.08206543459409943 1104 30-31 -0.12157386056581032 1104 32-33 -0.17999107208797938 1104 34-35 -0.016527222052772572 1104 36-37 -0.135260031413317 1104 38-39 -0.05210971481593418 1104 40-41 -0.06459066315260742 1104 42-43 -0.05545329049542147 1104 44-45 0.004317610635801827 1104 46-47 -0.03717213267854902 1104 48-49 -0.029588410941677523 1104 50-51 -0.16764707491780229 1104 52-53 -0.08417428029002849 1104 54-55 -0.007494337078753688 1104 56-57 -0.02178569223912774 1104 58-59 -0.01973192440379279 1104 60-61 -0.3104154963784538 1104 62-63 -0.41017144324343136 1104 64-65 -0.2347808304509975 1104 66-67 -0.20314191555018368 1104 68-69 -0.2757266654924564 1104 70-71 -0.4060461039856733 1104 72-73 -0.32986911974673205 1104 74-75 -0.5805114760154524 1104 76-77 -0.45743705283730307 1104 78-79 -0.2525410194973077 1104 80-81 -0.16313713340210612 1104 82-83 -0.35466655517804924 1104 84-85 -0.263245654094888 1104 86-87 -0.15724012342962368 1104 88-89 -0.16716310866431172 1104 90-91 -0.3862904603423374 1104 92-93 -0.2947564959196072 1104 94-95 -0.10954265769731464 1104 96-97 -0.2798921405179158 1104 98-99 -0.17105138414994414 1104 100-101 -0.3712035900313033 1105 1 0.38555290143477805 1105 2 0.5049021462167111 1105 3 0.10417898963318706 1105 4 0.1081481952618688 1105 5 0.030040901113665086 1105 6 0.09519832983339427 1105 7 0.14414658778257206 1105 8 0.10789321353502856 1105 9 0.13144174298648892 1105 10-11 0.1570239323820104 1105 12-13 0.0891588547662181 1105 14-15 0.1871717797317416 1105 16-17 0.16030184793811486 1105 18-19 0.26609032445767866 1105 20-21 0.2313780976327422 1105 22-23 0.10833400460190745 1105 24-25 0.050464286117815504 1105 26-27 0.09801298007391068 1105 28-29 0.17655181434572853 1105 30-31 0.23627068437551202 1105 32-33 0.17125660496615325 1105 34-35 -3.974736649610122E-4 1105 36-37 0.11020895599115477 1105 38-39 0.10760106424968541 1105 40-41 0.019490369315512623 1105 42-43 -0.06064153116753346 1105 44-45 -0.0976225042075356 1105 46-47 0.12100147388215277 1105 48-49 0.22969973221744056 1105 50-51 0.034929559916896835 1105 52-53 0.09903629646893108 1105 54-55 0.07167368725055923 1105 56-57 0.061458661972970674 1105 58-59 0.18342608843163788 1105 60-61 0.10508179008657237 1105 62-63 -0.0018534768609512753 1105 64-65 0.09495681541568146 1105 66-67 0.22953317518942384 1105 68-69 0.07403134233006625 1105 70-71 0.1332883966391485 1105 72-73 0.12257723915124075 1105 74-75 0.13998833480694017 1105 76-77 -0.044282226779248646 1105 78-79 0.021904129141390172 1105 80-81 -0.26689442748197933 1105 82-83 0.03462038249598365 1105 84-85 0.012281447238667198 1105 86-87 0.01562560104900257 1105 88-89 0.0706358904250095 1105 90-91 -0.16075959932760497 1105 92-93 0.12033546110783533 1105 94-95 0.3557326882231955 1105 96-97 0.3710733814153464 1105 98-99 0.4103158746366695 1105 100-101 0.6048795860518759 1106 1 0.34608441336008866 1106 2 0.35801864604263045 1106 3 0.143839394799695 1106 4 0.07558478573171357 1106 5 -0.03794794309025207 1106 6 0.0037284294457435863 1106 7 0.025435040401809772 1106 8 0.09979160573728763 1106 9 0.11528527642619224 1106 10-11 -0.016206339832699257 1106 12-13 0.06859210883123268 1106 14-15 0.1721291704466381 1106 16-17 0.10347999497859206 1106 18-19 0.14238942478479544 1106 20-21 -0.009871528682538155 1106 22-23 0.05321615397492252 1106 24-25 -0.036454841742028066 1106 26-27 -0.11390679366076029 1106 28-29 0.028603666407178707 1106 30-31 0.0010674306426636804 1106 32-33 0.0218431208220764 1106 34-35 -0.0958987401947411 1106 36-37 0.20847788842772275 1106 38-39 0.09771573471204675 1106 40-41 0.14780896645078911 1106 42-43 -0.040046489763881254 1106 44-45 0.14794537969738286 1106 46-47 0.07831210030692404 1106 48-49 -0.10782288877951629 1106 50-51 -0.09044470916808933 1106 52-53 0.2711375794839057 1106 54-55 0.0448068937053705 1106 56-57 0.08967096656508033 1106 58-59 0.11201952068428511 1106 60-61 0.08743772001714945 1106 62-63 -0.29276189673125685 1106 64-65 0.12864663064626924 1106 66-67 0.01511306621496189 1106 68-69 0.057792080767985254 1106 70-71 0.10878919567857892 1106 72-73 0.13890507783262152 1106 74-75 0.20572768925197238 1106 76-77 0.2337149707391113 1106 78-79 0.35606266496160544 1106 80-81 0.30812743999434744 1106 82-83 0.25790484668437585 1106 84-85 0.4001281743756202 1106 86-87 0.4311268429740309 1106 88-89 0.25596087618501073 1106 90-91 0.08100758801669272 1106 92-93 0.3964885322118832 1106 94-95 0.5347763259870639 1106 96-97 0.31975401292099903 1106 98-99 0.3888783664094575 1106 100-101 0.4623678588103495 1107 1 0.6210244256825312 1107 2 0.5876980459011172 1107 3 0.42329954180259577 1107 4 0.17450308215386912 1107 5 0.16171385155814733 1107 6 0.1839688374341577 1107 7 0.23908503486633492 1107 8 0.17528582718507835 1107 9 0.36131026547558776 1107 10-11 0.42031382132175565 1107 12-13 0.4028140259339139 1107 14-15 0.5330838108575193 1107 16-17 0.5029145945713722 1107 18-19 0.5701650431933771 1107 20-21 0.3959357839176505 1107 22-23 0.49679960844685667 1107 24-25 0.5093320918948692 1107 26-27 0.47309835312553616 1107 28-29 0.5381989872479949 1107 30-31 0.517102401593462 1107 32-33 0.6097238650784149 1107 34-35 0.5157621242535626 1107 36-37 0.5539297454585039 1107 38-39 0.6148845750545675 1107 40-41 0.6621086870297503 1107 42-43 0.5802101516000562 1107 44-45 0.6258529770134018 1107 46-47 0.6655522038443351 1107 48-49 0.6190830587249465 1107 50-51 0.5828432653783651 1107 52-53 0.4116632267732001 1107 54-55 0.6054565716852593 1107 56-57 0.5863833770949896 1107 58-59 0.44387096183258734 1107 60-61 0.43360994060814306 1107 62-63 0.30662887034085884 1107 64-65 0.2668272230381241 1107 66-67 0.42071249774597774 1107 68-69 0.3432773191061891 1107 70-71 0.25867189780757016 1107 72-73 0.44205449107251127 1107 74-75 0.8249598168951628 1107 76-77 0.6596414704912199 1107 78-79 0.7486394455787888 1107 80-81 0.911469602005603 1107 82-83 0.6672452349376243 1107 84-85 0.9524290658274062 1107 86-87 1.210035849428877 1107 88-89 1.1944883983148635 1107 90-91 0.8255871869115232 1107 92-93 0.948984368573683 1107 94-95 0.9488505963876896 1107 96-97 1.1239854004955454 1107 98-99 1.376753876033959 1107 100-101 1.470698294042915 1108 1 0.3019885405674394 1108 2 0.29002863554001834 1108 3 0.06900312429403144 1108 4 0.11800605388141605 1108 5 0.039954430142003616 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-1.0036632284046405 2316 56-57 -1.0431112415050663 2316 58-59 -1.141300252198839 2316 60-61 -1.0332949664638846 2316 62-63 -1.1892748522031482 2316 64-65 -1.1736755942526251 2316 66-67 -1.1025559857998068 2316 68-69 -1.1554459547380915 2316 70-71 -0.9703771664540426 2316 72-73 -1.099062485267929 2316 74-75 -1.240558178371895 2316 76-77 -1.2374613767679605 2316 78-79 -0.8920650773289758 2316 80-81 -0.8138338929333493 2316 82-83 -0.7005067969735883 2316 84-85 -1.013799291024256 2316 86-87 -1.1120957068110187 2316 88-89 -1.2302868061679995 2316 90-91 -1.246081763986755 2316 92-93 -1.3118362367885972 2316 94-95 -1.4185670356413276 2316 96-97 -1.6434240612611184 2316 98-99 -1.9367927584597062 2316 100-101 -2.6384208601756605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 28.0 5 89.0 6 188.0 7 485.0 8 1048.0 9 2038.0 10 3137.0 11 4998.0 12 7115.0 13 9475.0 14 11864.0 15 15792.0 16 19986.0 17 25234.0 18 21194.0 19 13982.0 20 12903.0 21 13232.0 22 13647.0 23 15713.0 24 18183.0 25 21750.0 26 26763.0 27 32567.0 28 40485.0 29 49509.0 30 60720.0 31 74189.0 32 92199.0 33 115966.0 34 150200.0 35 200568.0 36 272995.0 37 323777.0 38 173995.0 39 8600.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78496310641531 19.09352639477906 15.012107197040885 22.109403301764736 2 16.112917307253646 21.147054683973796 38.35907634682345 24.38095166194911 3 19.45675348358689 25.206545616897646 30.034498860642504 25.302202038872956 4 13.329943178049481 17.093177321478933 36.05376218258413 33.523117317887454 5 13.86040171764163 37.836912472312626 32.79781045984225 15.50487535020349 6 31.953547758158475 36.00614891547452 16.74226101509946 15.29804231126755 7 28.87941353451567 33.67523330723657 19.650055320828454 17.795297837419312 8 28.077099765129116 32.665816904233495 19.130222857746748 20.126860472890638 9 27.75432205409591 15.945416643750153 19.764871198420327 36.53539010373361 10-11 25.86904730131026 26.175873751739903 25.990068647060077 21.965010299889762 12-13 27.228297997501393 23.82430917599331 26.90653622576542 22.04085660073988 14-15 23.726150967710627 24.776708085418424 26.12662329784567 25.37051764902528 16-17 22.47815743897994 28.128403632842243 26.581011044754877 22.81242788342295 18-19 22.4359658714702 25.557117407405013 30.101081829534206 21.905834891590587 20-21 23.43214517565711 24.539047015155536 30.059645127130068 21.969162682057288 22-23 22.866584205395426 24.673731591797296 30.115097098686082 22.344587104121196 24-25 22.996237536637917 24.928422612307752 30.20080490187832 21.87453494917601 26-27 22.531132793712622 25.26493069259952 30.12947014939859 22.074466364289272 28-29 22.1661555151523 25.421996553501604 30.295297837419312 22.116550093926787 30-31 22.180369134528053 25.06805245559033 30.23753184925016 22.514046560631456 32-33 22.16923255692944 25.239889148579884 30.034409881187102 22.556468413303573 34-35 23.028912075392142 25.042744536221107 29.67554591957503 22.25279746881172 36-37 23.27208737975322 24.680202407941675 29.68823256477305 22.35947764753206 38-39 22.736244115077184 24.971240948683395 29.72231440874976 22.570200527489664 40-41 22.6463862376204 25.12236036198285 29.793002448555267 22.438250951841486 42-43 22.827045288482537 25.318680252043062 29.07287625138519 22.78139820808921 44-45 22.708095465972892 25.38696610432239 28.99650191754231 22.908436512162407 46-47 23.277914285421577 25.807520447838534 28.25185201960318 22.662713247136708 48-49 23.501829514733046 25.88706052753547 27.684921730310304 22.92618822742118 50-51 23.243636998902208 26.178052957250724 27.60149205123615 22.97681799261092 52-53 23.682091323957163 26.16117638590194 27.34054407012203 22.816188220018866 54-55 22.92265394915963 26.110092391200308 27.5400030682927 23.42725059134736 56-57 23.289229407098937 26.323589597311276 27.151441393883935 23.235739601705852 58-59 23.746643161269258 25.705141702336796 27.213842051386017 23.334373085007922 60-61 23.279870219682362 25.911361897980527 27.9551808191722 22.853587063164913 62-63 23.251083948078335 25.993562904037233 27.75470555597195 23.000647591912486 64-65 23.050956866970495 26.11065542165296 27.600544238817946 23.2378434725586 66-67 23.132401295391823 26.389375673335223 27.195976003114463 23.282247028158487 68-69 23.285467567402897 26.0297061787493 27.549577247441675 23.135249006406124 70-71 23.205753881276323 26.20716819556036 27.352153472780515 23.234924450382803 72-73 23.331505481399045 26.18411375601466 27.210568542829964 23.27381221975633 74-75 23.12905234722735 26.777775680929132 26.86954575967778 23.223626212165733 76-77 23.226539409381623 26.432540868983356 27.142283670119223 23.198636051515802 78-79 23.21891889341406 26.37221159458975 27.164498225207844 23.244371286788347 80-81 23.172353134520645 26.89097065103646 26.911462912915706 23.025213301527188 82-83 23.306721238985777 26.72016048144433 26.75909448776437 23.214023791805523 84-85 23.038235153758365 26.637995626277817 26.690463037032664 23.63330618293116 86-87 23.229698380874627 26.445220112830807 26.576282665878654 23.748798840415905 88-89 23.150929699247307 26.48661271915726 26.437911105262362 23.924546476333063 90-91 22.95529773421331 26.504378543451736 26.75540155187175 23.7849221704632 92-93 23.116181310741386 26.26225072576923 26.972517826100084 23.649050137389303 94-95 22.83936970000248 26.102437230450338 27.055317873131152 24.002875196416035 96-97 23.042805614377933 25.92546157815809 27.07003089320678 23.961701914257198 98-99 22.825848985789843 26.21294854317835 26.641539692674048 24.319662778357763 100-101 22.615033728811138 26.17996913865684 26.987335956430663 24.21766117610136 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 892.0 1 690.0 2 599.0 3 728.0 4 623.0 5 548.5 6 864.0 7 1828.5 8 2993.0 9 3496.0 10 3032.5 11 2503.5 12 2468.0 13 2563.0 14 2763.0 15 2996.0 16 3238.5 17 3464.0 18 3630.5 19 3855.5 20 4167.5 21 4600.0 22 5218.5 23 5901.5 24 6732.5 25 7908.0 26 9728.0 27 11852.0 28 13732.5 29 15987.0 30 18654.0 31 21849.5 32 26569.5 33 29277.0 34 30527.5 35 32277.0 36 35041.0 37 38617.5 38 40593.0 39 40797.5 40 41887.5 41 46611.0 42 51146.5 43 54233.5 44 56502.0 45 59271.5 46 61064.5 47 64274.5 48 75320.5 49 76798.5 50 68658.5 51 64585.0 52 63369.5 53 65439.5 54 66539.0 55 64182.0 56 59704.0 57 54884.0 58 52445.0 59 48651.5 60 41996.5 61 36354.0 62 31210.5 63 24301.0 64 18041.5 65 14543.0 66 11169.5 67 8800.0 68 6584.0 69 4474.5 70 3512.0 71 2821.5 72 2444.5 73 2303.0 74 1967.0 75 1669.5 76 1192.5 77 746.5 78 527.0 79 377.5 80 254.5 81 168.0 82 112.5 83 62.0 84 34.0 85 18.0 86 9.5 87 5.0 88 1.5 89 1.5 90 2.0 91 1.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0218177583094472 2 0.011700409136887238 3 0.004421352761404394 4 0.01331797722032787 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004205677016945643 10-11 2.426352125160948E-4 12-13 1.617568083440632E-4 14-15 5.3918936114687734E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015097302112112565 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.6959468057343867E-5 32-33 0.0043943932933470506 34-35 7.009461694909405E-4 36-37 0.003477771379397359 38-39 1.3479734028671933E-4 40-41 0.003936082336372205 42-43 0.010298516797905357 44-45 0.002615068401562355 46-47 0.0021028385084728217 48-49 0.009840205840930512 50-51 4.3135148891750187E-4 52-53 0.002183716912644853 54-55 0.009948043713159887 56-57 0.004151758080830956 58-59 0.001428851807039225 60-61 0.006335474993475808 62-63 0.003936082336372205 64-65 0.016337437642750384 66-67 0.0033429740391106395 68-69 2.6959468057343865E-4 70-71 0.0014558112750965688 72-73 0.0 74-75 5.3918936114687734E-5 76-77 0.0011053381903510986 78-79 0.010433314138192077 80-81 0.014962504771825846 82-83 0.01164649020077255 84-85 0.008168718821375192 86-87 0.009597570628414417 88-89 0.026312440823967616 90-91 0.022834669444570257 92-93 0.002803784677963762 94-95 0.008303516161661912 96-97 0.009867165308987855 98-99 0.010136759989561294 100-101 0.011457773924371143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1854636.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.629638806416 #Duplication Level Percentage of deduplicated Percentage of total 1 69.6890927120306 29.70820851060702 2 15.207896058010267 12.966142319169908 3 5.8474164483778335 7.478197534351288 4 2.9661045471398686 5.057758620265627 5 1.66394164552981 3.5466615671944637 6 0.9863148107395399 2.522774647874708 7 0.6965225378440092 2.078475294617283 8 0.452043638285028 1.5416365619863126 9 0.363744824528742 1.3955679438627095 >10 1.8368575674251486 14.312314774512696 >50 0.1660660461644534 4.93828676048405 >100 0.11292379487969656 8.913885796091794 >500 0.008020094825690606 2.3997684811079636 >1k 0.0026733649418968688 1.7018710892705953 >5k 2.5460618494255893E-4 0.8385622471750396 >10k+ 1.2730309247127946E-4 0.5998878514284809 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11054 0.5960199198117583 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9278 0.5002598892720728 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6174 0.3328955115720821 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3379 0.18219208513152985 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2203 0.11878341626065708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.3918936114687734E-5 2 0.0 0.0 0.0 0.0 5.3918936114687734E-5 3 0.0 0.0 0.0 0.0 5.3918936114687734E-5 4 0.0 0.0 0.0 0.0 5.3918936114687734E-5 5 0.0 0.0 0.0 0.0 5.3918936114687734E-5 6 0.0 0.0 0.0 0.0 5.3918936114687734E-5 7 0.0 0.0 0.0 0.0 5.3918936114687734E-5 8 0.0 0.0 0.0 0.0 5.3918936114687734E-5 9 0.0 0.0 0.0 5.3918936114687734E-5 5.3918936114687734E-5 10-11 0.0 0.0 0.0 5.3918936114687734E-5 5.3918936114687734E-5 12-13 0.0 0.0 0.0 5.3918936114687734E-5 1.0783787222937547E-4 14-15 0.0 0.0 0.0 5.3918936114687734E-5 1.0783787222937547E-4 16-17 0.0 0.0 0.0 5.3918936114687734E-5 1.0783787222937547E-4 18-19 0.0 0.0 0.0 5.3918936114687734E-5 1.0783787222937547E-4 20-21 0.0 0.0 0.0 1.0783787222937547E-4 1.617568083440632E-4 22-23 0.0 0.0 0.0 2.1567574445875094E-4 2.1567574445875094E-4 24-25 0.0 0.0 0.0 3.235136166881264E-4 2.1567574445875094E-4 26-27 0.0 0.0 0.0 5.391893611468773E-4 2.1567574445875094E-4 28-29 0.0 0.0 0.0 0.0010514192542364108 2.1567574445875094E-4 30-31 0.0 0.0 0.0 0.00285770361407845 2.1567574445875094E-4 32-33 0.0 0.0 0.0 0.005176217867010023 2.1567574445875094E-4 34-35 0.0 0.0 0.0 0.00800696201303113 2.6959468057343865E-4 36-37 0.0 0.0 0.0 0.011673449668829893 2.6959468057343865E-4 38-39 0.0 0.0 0.0 0.01701142434418398 2.6959468057343865E-4 40-41 0.0 0.0 0.0 0.023131223593201036 2.6959468057343865E-4 42-43 0.0 0.0 0.0 0.028900549757472624 2.6959468057343865E-4 44-45 0.0 0.0 0.0 0.03305230783830358 2.6959468057343865E-4 46-47 0.0 0.0 0.0 0.039603458576238135 2.6959468057343865E-4 48-49 0.0 0.0 0.0 0.04539974420856707 2.6959468057343865E-4 50-51 0.0 0.0 0.0 0.05130386771312538 2.6959468057343865E-4 52-53 0.0 0.0 0.0 0.0664281292932953 2.6959468057343865E-4 54-55 0.0 0.0 0.0 0.09004462331152852 2.6959468057343865E-4 56-57 0.0 0.0 0.0 0.12161416040667819 2.6959468057343865E-4 58-59 5.3918936114687734E-5 0.0 0.0 0.16332045749138913 2.6959468057343865E-4 60-61 5.3918936114687734E-5 0.0 0.0 0.21268324350438578 2.6959468057343865E-4 62-63 5.3918936114687734E-5 0.0 0.0 0.2450885241093131 2.6959468057343865E-4 64-65 5.3918936114687734E-5 0.0 0.0 0.283344009282684 3.235136166881264E-4 66-67 5.3918936114687734E-5 0.0 0.0 0.35484051857075993 3.235136166881264E-4 68-69 5.3918936114687734E-5 0.0 0.0 0.45273034708697557 3.235136166881264E-4 70-71 5.3918936114687734E-5 0.0 0.0 0.5502966619865031 3.5047308474547026E-4 72-73 5.3918936114687734E-5 0.0 0.0 0.6627446032536843 4.04392020860158E-4 74-75 5.3918936114687734E-5 0.0 0.0 0.7676438934648093 4.5831095697484574E-4 76-77 5.3918936114687734E-5 0.0 0.0 0.8626760183669464 4.852704250321896E-4 78-79 5.3918936114687734E-5 0.0 0.0 0.9541764529535715 4.852704250321896E-4 80-81 5.3918936114687734E-5 0.0 0.0 1.0448950629665337 4.852704250321896E-4 82-83 5.3918936114687734E-5 0.0 0.0 1.1764303076183142 4.852704250321896E-4 84-85 5.3918936114687734E-5 0.0 0.0 1.3858784149558188 5.391893611468773E-4 86-87 5.3918936114687734E-5 0.0 0.0 1.6604875565879236 5.391893611468773E-4 88-89 5.3918936114687734E-5 0.0 0.0 1.9767490763686244 5.391893611468773E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15700 0.0 28.924969 1 GGTATCA 10925 0.0 27.989775 1 GTAAGGT 765 0.0 26.079609 4 GTACATG 11975 0.0 25.415295 1 AGGTAAG 735 0.0 25.20794 2 TAAGGTG 800 0.0 24.93392 5 GGTAAGG 790 0.0 24.654343 3 TACATGG 12420 0.0 24.327393 2 ACATGGG 12335 0.0 23.338346 3 ATCAACG 19550 0.0 23.229923 3 TCAACGC 19575 0.0 23.174736 4 TATCAAC 19845 0.0 22.933718 2 CAACGCA 19860 0.0 22.813944 5 AACGCAG 20070 0.0 22.62256 6 GAGTACT 10670 0.0 21.986036 12-13 AGTACTT 10860 0.0 21.404608 12-13 GTACTTT 11325 0.0 20.672506 14-15 ACGCAGA 22085 0.0 20.558514 7 CATGGGA 7595 0.0 20.389334 4 CGCAGAG 22430 0.0 20.157604 8 >>END_MODULE