FastQCFastQC Report
Thu 26 May 2016
SRR936469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859358
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92670.49839783409112176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70820.3808841546383214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43680.2349197948969483No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30540.1642502412122894No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18810.10116395013762816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144950.029.340661
GTACATG126600.027.6629731
TACATGG128850.026.922492
GGTATCA100350.026.8491631
ACATGGG129300.026.020253
ATCAACG176200.024.056683
TCAACGC176450.024.023894
TATCAAC178450.023.806622
CAACGCA180050.023.56745
AACGCAG182900.023.2774816
CATGGGG51250.022.9035724
GAGTACT81900.022.83289512-13
GCGCGAC1050.002191351322.6285889
TAAGGTG8300.022.327495
GTGTAAG7050.022.2428231
AGTACTT83600.021.74329212-13
CATGGGA74650.021.262624
ATGGGAG28100.021.1376675
ACGCAGA201900.020.9474147
GTACTTT89050.020.94566714-15
Produced by FastQC (version 0.11.5)