##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936469_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1859358 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.356958154373714 31.0 31.0 34.0 30.0 34.0 2 31.382683162683033 31.0 31.0 34.0 28.0 34.0 3 31.43172912370829 31.0 31.0 34.0 28.0 34.0 4 35.07571000313011 37.0 35.0 37.0 32.0 37.0 5 35.04677205788234 37.0 35.0 37.0 32.0 37.0 6 35.18698335662094 37.0 35.0 37.0 32.0 37.0 7 35.03732955138279 37.0 35.0 37.0 32.0 37.0 8 34.97917668356497 37.0 35.0 37.0 32.0 37.0 9 36.43584990087977 39.0 37.0 39.0 32.0 39.0 10-11 36.35253162650764 39.0 36.0 39.0 32.0 39.0 12-13 36.233342368710055 39.0 35.0 39.0 32.0 39.0 14-15 37.25898267036257 39.5 36.5 41.0 31.5 41.0 16-17 37.1435928422606 39.0 36.0 41.0 31.0 41.0 18-19 37.099851131412024 39.0 36.0 41.0 31.0 41.0 20-21 37.0228307297465 39.0 36.0 41.0 31.0 41.0 22-23 36.893799902977264 39.0 36.0 41.0 30.5 41.0 24-25 37.001794974394386 39.0 36.0 41.0 31.0 41.0 26-27 36.97355350610265 39.0 36.0 41.0 30.5 41.0 28-29 36.87321268954123 39.0 36.0 41.0 30.0 41.0 30-31 36.65670435709529 39.0 36.0 41.0 30.0 41.0 32-33 36.45619778439655 39.0 35.0 40.5 30.0 41.0 34-35 36.21908637282331 39.0 35.0 40.0 30.0 41.0 36-37 36.0044418557373 38.0 35.0 40.0 29.0 41.0 38-39 35.7747426800003 38.0 35.0 40.0 28.5 41.0 40-41 35.55934171902345 38.0 35.0 40.0 27.0 41.0 42-43 35.276716210649056 38.0 34.0 40.0 26.5 41.0 44-45 34.96147756376126 38.0 34.0 40.0 25.5 41.0 46-47 34.573132231662754 38.0 33.5 40.0 24.0 41.0 48-49 34.3823384200353 38.0 33.0 40.0 23.0 41.0 50-51 33.42322242408402 37.0 32.0 39.5 22.5 40.5 52-53 33.544413985902665 37.5 33.0 39.5 22.5 40.5 54-55 33.91891314098737 38.0 33.0 40.0 22.5 41.0 56-57 33.80448224602255 37.5 33.0 40.0 22.0 41.0 58-59 33.518073173643806 37.0 33.0 40.0 20.5 41.0 60-61 33.18210559773857 36.5 32.5 40.0 20.0 41.0 62-63 32.82996442858234 36.0 32.0 39.0 18.5 41.0 64-65 32.47294146689342 35.5 32.0 39.0 18.0 41.0 66-67 32.07514287189449 35.0 31.0 39.0 16.0 40.5 68-69 31.668574045450097 35.0 31.0 38.0 12.5 40.0 70-71 31.23849091998421 35.0 31.0 37.5 9.5 40.0 72-73 30.860604843177054 35.0 30.0 37.0 7.5 39.0 74-75 30.449309385282447 35.0 30.0 36.0 7.0 39.0 76-77 30.042237428187576 34.0 29.5 36.0 3.5 38.5 78-79 29.605028187148466 34.0 29.0 35.0 2.0 37.5 80-81 29.159273792351982 34.0 29.0 35.0 2.0 37.0 82-83 28.699705758654332 34.0 28.5 35.0 2.0 36.5 84-85 28.401214021183655 34.0 27.5 35.0 2.0 36.0 86-87 28.086081862664425 34.0 27.0 35.0 2.0 36.0 88-89 27.689325831819367 33.5 26.5 35.0 2.0 35.0 90-91 27.287996986056477 33.0 26.0 35.0 2.0 35.0 92-93 26.93707559275836 33.0 25.0 35.0 2.0 35.0 94-95 26.459467730259583 33.0 25.0 35.0 2.0 35.0 96-97 25.859973980266307 33.0 23.5 35.0 2.0 35.0 98-99 25.135944503425375 32.0 19.0 35.0 2.0 35.0 100-101 22.6410790713784 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3333893574337985 1101 2 0.530508545179444 1101 3 0.5253770439441006 1101 4 0.5323277540481115 1101 5 0.5211242408085965 1101 6 0.44374804815387847 1101 7 0.5594518753846458 1101 8 0.5371912052645058 1101 9 0.6954697872430273 1101 10-11 0.7074261109099282 1101 12-13 0.7868243612842392 1101 14-15 0.94959268706031 1101 16-17 0.8494235800543066 1101 18-19 0.8985660901915864 1101 20-21 0.8837873046946285 1101 22-23 0.8728717162658626 1101 24-25 0.8219962568670169 1101 26-27 0.6834901055883407 1101 28-29 0.7389324643315334 1101 30-31 0.7685905471978458 1101 32-33 0.6434795058565399 1101 34-35 0.6733136303170681 1101 36-37 0.47532280850712993 1101 38-39 0.4730930546342975 1101 40-41 0.5747791311426553 1101 42-43 0.57161655769508 1101 44-45 0.5415819275422109 1101 46-47 0.4053291735197959 1101 48-49 0.6016846495282948 1101 50-51 0.6055941910429183 1101 52-53 0.6501042618290072 1101 54-55 0.7911285897484603 1101 56-57 0.6605381804604846 1101 58-59 0.42535886623995367 1101 60-61 0.5888277828915491 1101 62-63 0.5629961091214639 1101 64-65 0.5647996202186008 1101 66-67 0.6403408378822917 1101 68-69 0.7853763285781703 1101 70-71 0.924202794157619 1101 72-73 0.6099240671950348 1101 74-75 0.5801383819067389 1101 76-77 0.5817082738245567 1101 78-79 0.5290803968909685 1101 80-81 0.6201968791093897 1101 82-83 0.6825642295201568 1101 84-85 0.6592822005661141 1101 86-87 0.5768606483707863 1101 88-89 0.6877680961938033 1101 90-91 0.4457285688156709 1101 92-93 0.3698452272726911 1101 94-95 0.5408445019110957 1101 96-97 0.29195923581312755 1101 98-99 0.41134791355331757 1101 100-101 0.7666565079212653 1102 1 0.3193410181096077 1102 2 0.381046836690647 1102 3 0.18298340855008988 1102 4 0.3665645871302985 1102 5 0.4129846456058317 1102 6 0.278405949316749 1102 7 0.3729733735165368 1102 8 0.17242630371827516 1102 9 0.3406058056445147 1102 10-11 0.44784194094417984 1102 12-13 0.43344474588842985 1102 14-15 0.4191153542634325 1102 16-17 0.2140594282825461 1102 18-19 0.5625268124346761 1102 20-21 0.4345662426870476 1102 22-23 0.27277062450009737 1102 24-25 0.027118661408685796 1102 26-27 0.08541919964669376 1102 28-29 0.11244161383221041 1102 30-31 0.09530888580085417 1102 32-33 -0.1211829121724648 1102 34-35 -0.27907164191584144 1102 36-37 -0.13498753941808417 1102 38-39 -0.3135244958578127 1102 40-41 -0.38484881573292284 1102 42-43 -0.31232756474868495 1102 44-45 -0.7115051669994656 1102 46-47 -0.4512275574331355 1102 48-49 -0.2869471449074794 1102 50-51 -0.37511803144500533 1102 52-53 -0.4513592281016727 1102 54-55 -0.26322006423809086 1102 56-57 -0.21985963271838926 1102 58-59 -0.18881207373633657 1102 60-61 -0.3406997427894254 1102 62-63 -0.3011914686110231 1102 64-65 -0.30229583242326186 1102 66-67 -0.23496392405861144 1102 68-69 -0.25045152947836513 1102 70-71 -0.25003955532129396 1102 72-73 -0.29756975532628616 1102 74-75 -0.4707860573755731 1102 76-77 -0.25940532978691166 1102 78-79 -0.4199617617914768 1102 80-81 -0.19187858924661327 1102 82-83 -0.18432764783870326 1102 84-85 -0.296092553821385 1102 86-87 -0.09570059185690027 1102 88-89 -0.19821835826872203 1102 90-91 -0.15165481190047458 1102 92-93 -0.24938928411499717 1102 94-95 -0.1546063686294481 1102 96-97 -0.19364246262975726 1102 98-99 -0.32119148882860316 1102 100-101 -0.2390134875618699 1103 1 0.2326388872382381 1103 2 0.36208455715944865 1103 3 0.3201224012620152 1103 4 0.36076552546243335 1103 5 0.39181118700053474 1103 6 0.4023575714720167 1103 7 0.36187883383667696 1103 8 0.2694054056003736 1103 9 0.47249279519243714 1103 10-11 0.5030417139088641 1103 12-13 0.4139649949026989 1103 14-15 0.466469728618101 1103 16-17 0.5483399497391943 1103 18-19 0.4769121565803758 1103 20-21 0.5088593235442218 1103 22-23 0.5045616070920431 1103 24-25 0.6914083005070637 1103 26-27 0.42994468979541267 1103 28-29 0.36126930247984035 1103 30-31 0.3366806380183718 1103 32-33 0.5373275912300457 1103 34-35 0.4665189820086013 1103 36-37 0.5379892196527578 1103 38-39 0.37093083071679445 1103 40-41 0.2897473814255136 1103 42-43 0.5713609138127822 1103 44-45 0.6239872158448776 1103 46-47 0.700084235723196 1103 48-49 0.8003823521730595 1103 50-51 0.6945582620826798 1103 52-53 0.55698613126021 1103 54-55 0.7607327655518432 1103 56-57 0.7818944566344967 1103 58-59 0.5833591930120505 1103 60-61 0.4578058093109476 1103 62-63 0.6106469740233109 1103 64-65 0.7091881694364943 1103 66-67 0.47814615787939374 1103 68-69 0.6891866631755938 1103 70-71 0.7283552566801816 1103 72-73 0.7007606897474723 1103 74-75 0.4095397624574133 1103 76-77 0.4078629402160452 1103 78-79 0.5188685232706902 1103 80-81 0.545579822834565 1103 82-83 0.5414004049107781 1103 84-85 0.48744798949345025 1103 86-87 0.6888302118843477 1103 88-89 0.6331481407379975 1103 90-91 0.7161623280583456 1103 92-93 0.6524384709525393 1103 94-95 0.48332241715428026 1103 96-97 0.5726264896867868 1103 98-99 0.6400802712385456 1103 100-101 1.168014946196351 1104 1 0.18156244675807187 1104 2 0.2891684116576023 1104 3 0.2912854549717565 1104 4 0.3094277559741627 1104 5 0.3928744214066029 1104 6 0.20223237556252371 1104 7 0.5184388784231757 1104 8 0.42803979282108173 1104 9 0.3881337901043125 1104 10-11 0.39519940025122224 1104 12-13 0.3489569025462842 1104 14-15 0.4027433817439885 1104 16-17 0.23784594948554627 1104 18-19 0.47168615818371507 1104 20-21 0.5114167641488834 1104 22-23 0.31296986211006583 1104 24-25 0.2728788404900513 1104 26-27 0.045949637128195775 1104 28-29 0.22965303026949613 1104 30-31 0.41674795990800106 1104 32-33 -0.04786368740881386 1104 34-35 -0.06439125973817994 1104 36-37 -0.04122674678170313 1104 38-39 0.06317275668597944 1104 40-41 0.09893403852019844 1104 42-43 0.15675837010183358 1104 44-45 0.12504917036712015 1104 46-47 0.159511452801965 1104 48-49 0.10478352361582921 1104 50-51 0.14779561188540669 1104 52-53 0.32054306805606103 1104 54-55 0.3355558216119263 1104 56-57 0.2932693660245391 1104 58-59 0.11048761073821112 1104 60-61 0.2526164621331546 1104 62-63 0.3757901523006595 1104 64-65 0.309239481142896 1104 66-67 0.4445290781787037 1104 68-69 0.3752958512289233 1104 70-71 0.45942129519537644 1104 72-73 0.3313424667614555 1104 74-75 0.05811298286620925 1104 76-77 0.1993891784163857 1104 78-79 0.22158897453445547 1104 80-81 0.3418770025523621 1104 82-83 0.3194186327836235 1104 84-85 0.35206420469079447 1104 86-87 0.2212004520254638 1104 88-89 0.39073453580556006 1104 90-91 0.3592471738847003 1104 92-93 0.3141286390012894 1104 94-95 0.16530828490969895 1104 96-97 0.49168710149453076 1104 98-99 0.7713761070783178 1104 100-101 1.0799601020105705 1105 1 0.201620242228298 1105 2 0.23835056181363612 1105 3 0.302980109404956 1105 4 0.34415271797050906 1105 5 0.5376370224458356 1105 6 0.3407435391941931 1105 7 0.3013178818953932 1105 8 0.25636237792963357 1105 9 0.43756331459587017 1105 10-11 0.39703425684702864 1105 12-13 0.28715737579226897 1105 14-15 0.07567846982085058 1105 16-17 0.27804088999791077 1105 18-19 0.19713278291703062 1105 20-21 0.22888916592369668 1105 22-23 -0.061834860813853254 1105 24-25 0.15622585451541227 1105 26-27 0.1528089693857666 1105 28-29 0.04642984364677716 1105 30-31 -0.017194631670058413 1105 32-33 -0.03933682214461243 1105 34-35 -0.08231923790641105 1105 36-37 -0.0522455859360349 1105 38-39 -0.05471359509415663 1105 40-41 0.030331133227356588 1105 42-43 0.302759595754722 1105 44-45 -0.07726489220007693 1105 46-47 0.1385535831007374 1105 48-49 0.2739770281229781 1105 50-51 0.45283640153868276 1105 52-53 0.3239438808574491 1105 54-55 0.3398043542875868 1105 56-57 0.3566743484764814 1105 58-59 0.33183657215865736 1105 60-61 0.22294416301027553 1105 62-63 0.1776652563082024 1105 64-65 0.35854185933954597 1105 66-67 0.38002220012508303 1105 68-69 0.22271235070444462 1105 70-71 0.1816737198565228 1105 72-73 0.14260267352376843 1105 74-75 0.18969151814089358 1105 76-77 -0.02995609295680879 1105 78-79 0.08813982276852528 1105 80-81 0.0303241723319303 1105 82-83 0.0343173738483209 1105 84-85 0.13930848637966875 1105 86-87 0.1437559710160734 1105 88-89 0.20989499162502767 1105 90-91 0.33914578873800494 1105 92-93 0.358943968356936 1105 94-95 0.3480198479303205 1105 96-97 0.3958850561220011 1105 98-99 0.27632663221121234 1105 100-101 0.6630957820656214 1106 1 0.10400934728666655 1106 2 0.18892838282530988 1106 3 0.1715170743855019 1106 4 0.22964688139463618 1106 5 0.34609811194000173 1106 6 0.23807427849380502 1106 7 0.30996963286816026 1106 8 0.18979039349382987 1106 9 0.4485496959188282 1106 10-11 0.3968007938120124 1106 12-13 0.5165999827961585 1106 14-15 0.5223117383033369 1106 16-17 0.6262140417488808 1106 18-19 0.4239557401154812 1106 20-21 0.17498644218829185 1106 22-23 0.134910275372917 1106 24-25 0.2556704070445974 1106 26-27 -0.007411847734850596 1106 28-29 0.3709278981214865 1106 30-31 0.2724366912871474 1106 32-33 0.12463691326394866 1106 34-35 0.08100566481731875 1106 36-37 0.08721258527019415 1106 38-39 0.07539632708483168 1106 40-41 -0.044163030196763486 1106 42-43 -0.22463242759158675 1106 44-45 0.22260767589330044 1106 46-47 0.07058665703069522 1106 48-49 0.28780971294788316 1106 50-51 0.2074910707993851 1106 52-53 0.4701102232698915 1106 54-55 0.49165649436540804 1106 56-57 0.3880936091768703 1106 58-59 0.3432490235205208 1106 60-61 0.3986163419986042 1106 62-63 0.5495815987206498 1106 64-65 0.35686189825005243 1106 66-67 0.3945747293079691 1106 68-69 0.4137629343620297 1106 70-71 0.45488189106274746 1106 72-73 0.42180306262882894 1106 74-75 0.28809424187629773 1106 76-77 0.29624429614824876 1106 78-79 0.5994472157646342 1106 80-81 0.7524817598805704 1106 82-83 0.865896167622644 1106 84-85 0.818094478597569 1106 86-87 0.87348457023786 1106 88-89 1.1032286492125785 1106 90-91 1.0931350883489053 1106 92-93 1.229515952792731 1106 94-95 1.3253593420937442 1106 96-97 1.409697312931339 1106 98-99 1.6879949201489524 1106 100-101 2.0411317742835173 1107 1 0.22837121499094337 1107 2 0.27823383029612714 1107 3 0.15998400045554106 1107 4 0.16856906038296415 1107 5 0.38004947380770204 1107 6 0.34366182713193894 1107 7 0.41853578072807807 1107 8 0.3400199654782696 1107 9 0.4292948321055974 1107 10-11 0.21464126073808387 1107 12-13 0.3647351968039416 1107 14-15 0.3427018161243467 1107 16-17 0.4477879717099711 1107 18-19 0.5390588529559466 1107 20-21 0.5467393604762307 1107 22-23 0.6947312870460678 1107 24-25 0.5351169050990663 1107 26-27 0.5068945724986094 1107 28-29 0.4661769253588375 1107 30-31 0.46675478467235365 1107 32-33 0.42077990937288945 1107 34-35 0.5001223963348309 1107 36-37 0.4642913790445107 1107 38-39 0.5177572220264679 1107 40-41 0.39390117213786624 1107 42-43 0.4046564829142554 1107 44-45 0.498610594181244 1107 46-47 0.4211411317388709 1107 48-49 0.477527611780566 1107 50-51 0.32880916418459805 1107 52-53 0.6121248147095812 1107 54-55 0.5662148601241555 1107 56-57 0.6905751266865963 1107 58-59 0.8196770390847306 1107 60-61 0.6339363809091125 1107 62-63 0.7014979411330984 1107 64-65 0.8882111200399336 1107 66-67 0.7263257020706106 1107 68-69 0.8049107942842149 1107 70-71 0.946021968883759 1107 72-73 0.8451804945354411 1107 74-75 0.764837432537778 1107 76-77 0.6439252300003879 1107 78-79 0.7279744531187191 1107 80-81 0.8960829271957458 1107 82-83 1.0124302143152484 1107 84-85 0.8927131556403687 1107 86-87 0.8314991616775522 1107 88-89 0.7531829293682186 1107 90-91 0.6873948159753631 1107 92-93 0.8518914391740537 1107 94-95 0.7548778245840175 1107 96-97 0.63931890826208 1107 98-99 0.7186904454407852 1107 100-101 1.282502396851612 1108 1 -0.022665327388008194 1108 2 0.1444443988413262 1108 3 0.05199955686798319 1108 4 0.1858991336468918 1108 5 0.2538843982262833 1108 6 0.19345666087618696 1108 7 -0.07257741417888752 1108 8 -0.008745142041703957 1108 9 0.14363027649940818 1108 10-11 -0.09220571269450062 1108 12-13 0.22029893174511272 1108 14-15 0.22654772428932546 1108 16-17 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2316 82-83 -2.7267384477619707 2316 84-85 -2.580747150361706 2316 86-87 -3.2762133935630438 2316 88-89 -3.608582436760276 2316 90-91 -3.239334368664675 2316 92-93 -3.795816626392785 2316 94-95 -4.0673046850555785 2316 96-97 -5.125932777566398 2316 98-99 -6.505822953154215 2316 100-101 -6.880489266440463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3936.0 3 1737.0 4 1727.0 5 1908.0 6 2228.0 7 2504.0 8 3259.0 9 4110.0 10 5109.0 11 6514.0 12 8475.0 13 10360.0 14 11851.0 15 14485.0 16 17822.0 17 22772.0 18 19028.0 19 13056.0 20 13456.0 21 14384.0 22 15717.0 23 17778.0 24 20513.0 25 23569.0 26 27453.0 27 32028.0 28 37925.0 29 45821.0 30 55414.0 31 67771.0 32 83662.0 33 107377.0 34 141736.0 35 191726.0 36 259186.0 37 311303.0 38 213500.0 39 28153.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.33982677111977 18.1919914865091 14.109723101721258 22.358458640649875 2 15.962634016708716 20.287049937866815 39.132179610632036 24.618136434792433 3 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23.289577922619237 26.200566756895284 27.538963864273068 22.97089145621241 48-49 23.44432450922022 26.25142725605955 27.06480858499562 23.239439649724606 50-51 23.24188313740064 26.572635826035324 26.99926237842219 23.186218658141843 52-53 23.650048660825576 26.52919905007184 26.71749587277137 23.103256416331213 54-55 22.938192293749307 26.35425216408189 26.99271951971205 23.714836022456755 56-57 23.28307829270031 26.731351372431945 26.57470949036252 23.41086084450523 58-59 23.593690412604975 26.093315238935872 26.707954596186728 23.605039752272422 60-61 23.204185569799524 26.322600737595337 27.36953106783505 23.103682624770087 62-63 23.201856023699964 26.422198060662843 27.232554530915397 23.1433913847218 64-65 22.975260526961883 26.57338708311182 27.0494926604984 23.401859729427894 66-67 22.955582358802246 26.876538777529912 26.6583836566531 23.50949520701474 68-69 23.10314889386886 26.44257510212469 27.138136483984415 23.316139520022034 70-71 23.120191260430982 26.569709747773963 26.85668107215563 23.453417919639424 72-73 23.163855937490588 26.610172408600206 26.748530632968038 23.47744102094117 74-75 22.970370250849793 27.198619895873673 26.517953186179593 23.31305666709694 76-77 23.164993342650845 26.816706006566722 26.575556946219752 23.442743704562684 78-79 23.15739000438081 26.96350587563927 26.645581041116433 23.233523078863485 80-81 23.137614012723436 27.35888509238678 26.422444454305488 23.081056440584295 82-83 23.230244616079688 27.24150728254556 26.25833673974312 23.269911361631635 84-85 22.97466704930524 27.116200646415333 26.230304490830257 23.67882781344917 86-87 23.313675027497425 26.888286500310603 26.174832527907533 23.623205944284432 88-89 23.15415467477893 26.984234981656513 26.01480817730657 23.846802166257987 90-91 22.899650726138958 26.950130318842824 26.410487681830553 23.739731273187665 92-93 23.14754100124312 26.858160900125817 26.514917196020072 23.479380902610984 94-95 22.854016222003544 26.603139137069448 26.624735126401085 23.918109514525927 96-97 23.00890286879779 26.481113138293523 26.608158668141158 23.901825324767533 98-99 22.8429664572176 26.795009695122097 26.145413651818565 24.216610195841735 100-101 22.787400824955593 26.626056448096207 26.49819451853514 24.088348208413063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 683.0 1 504.5 2 438.5 3 579.0 4 555.0 5 575.0 6 870.0 7 1531.5 8 2416.5 9 2872.5 10 2587.0 11 2274.5 12 2308.5 13 2438.5 14 2560.5 15 2705.5 16 2842.0 17 2968.5 18 3120.0 19 3340.0 20 3675.0 21 4097.5 22 4641.5 23 5421.0 24 6370.5 25 7641.0 26 9422.5 27 11518.0 28 13508.0 29 15549.5 30 18194.5 31 21495.0 32 26426.0 33 29315.0 34 30646.0 35 32583.0 36 35285.0 37 38541.5 38 40341.5 39 41030.0 40 42209.5 41 46601.0 42 50864.5 43 54434.0 44 57225.5 45 60176.0 46 62114.5 47 64723.5 48 74878.5 49 76710.5 50 69191.0 51 65163.5 52 64112.5 53 66074.0 54 66936.5 55 64612.5 56 60693.5 57 55773.5 58 52737.0 59 48789.5 60 42595.0 61 37459.0 62 32388.0 63 25328.5 64 18830.5 65 15059.5 66 11541.5 67 9071.0 68 6793.5 69 4699.5 70 3722.0 71 2981.0 72 2473.0 73 2302.5 74 1862.5 75 1502.5 76 1150.0 77 689.5 78 447.0 79 300.0 80 205.0 81 132.0 82 77.0 83 56.0 84 34.5 85 29.0 86 20.5 87 13.0 88 12.5 89 5.5 90 4.0 91 5.5 92 6.5 93 3.5 94 2.0 95 1.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03554990485963435 2 0.024094337938148542 3 0.023717863907864973 4 0.052437454218068814 5 0.05674001456416677 6 0.04990970001473627 7 0.05351309430459331 8 0.04496175561672362 9 0.04582226768594321 10-11 0.05472318940193335 12-13 0.0491029699498429 14-15 0.04264912943069597 16-17 0.05216854419643769 18-19 0.05561059247331606 20-21 0.012746335025315188 22-23 0.02258844181701426 24-25 0.006265603504005146 26-27 0.011670694938790702 28-29 0.006346276510494482 30-31 0.009653869776557284 32-33 0.0040336503244668325 34-35 0.008443774679217236 36-37 0.009788324787372845 38-39 0.012289187988542283 40-41 0.0017748061427654061 42-43 0.0018285881470916307 44-45 0.0023395171881907626 46-47 3.7647403028357097E-4 48-49 0.001129422090850713 50-51 1.8823701514178548E-4 52-53 0.0032000292574103535 54-55 0.00752948060567142 56-57 0.018312772473079417 58-59 0.01177825894744315 60-61 0.011213547902017793 62-63 0.006158039495352697 64-65 8.067300648933665E-5 66-67 0.007018551564572288 68-69 0.019146393540135895 70-71 0.00607736648886336 72-73 0.002904228233616119 74-75 0.004194996337445505 76-77 0.007233679581877185 78-79 0.006104257491026473 80-81 0.010245471824145754 82-83 0.006346276510494482 84-85 0.01809764445577452 86-87 0.005001726402338871 88-89 0.00618493049751581 90-91 0.0038723043114881586 92-93 0.01769427942332784 94-95 0.01164380393662759 96-97 0.009788324787372845 98-99 0.02331449887541829 100-101 0.03831967808243491 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1859358.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.7510596770349 #Duplication Level Percentage of deduplicated Percentage of total 1 71.63712730401868 32.05837359073486 2 14.200251511679548 12.709526056561533 3 5.632952451281131 7.5624177391554595 4 2.7331494434795136 4.892453354056258 5 1.5074484045880079 3.3729956756884496 6 0.908896792384213 2.4404456757750914 7 0.6421357153788417 2.0115377603771836 8 0.45561230281936094 1.6311306682448414 9 0.3310034599796456 1.333148003176874 >10 1.6811426723774832 13.747721894624295 >50 0.15588038126181383 4.83650917498751 >100 0.10507868214884697 8.73081365787619 >500 0.006415669687827566 1.9711319654825497 >1k 0.0026631081723057825 1.8161478075897426 >5k 2.4210074293688934E-4 0.8856469756691733 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9267 0.49839783409112176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7082 0.3808841546383214 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4368 0.2349197948969483 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3054 0.1642502412122894 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1881 0.10116395013762816 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.3782004326224425E-5 2 0.0 0.0 0.0 0.0 5.3782004326224425E-5 3 0.0 0.0 0.0 0.0 5.3782004326224425E-5 4 0.0 0.0 0.0 0.0 5.3782004326224425E-5 5 0.0 0.0 0.0 0.0 5.3782004326224425E-5 6 0.0 0.0 0.0 0.0 5.3782004326224425E-5 7 0.0 0.0 0.0 5.3782004326224425E-5 5.3782004326224425E-5 8 0.0 0.0 0.0 5.3782004326224425E-5 5.3782004326224425E-5 9 0.0 0.0 0.0 5.3782004326224425E-5 5.3782004326224425E-5 10-11 0.0 0.0 0.0 5.3782004326224425E-5 5.3782004326224425E-5 12-13 0.0 0.0 0.0 5.3782004326224425E-5 1.613460129786733E-4 14-15 0.0 0.0 0.0 5.3782004326224425E-5 1.613460129786733E-4 16-17 0.0 0.0 0.0 1.0756400865244885E-4 1.613460129786733E-4 18-19 0.0 0.0 0.0 1.0756400865244885E-4 2.151280173048977E-4 20-21 0.0 0.0 0.0 1.882370151417855E-4 2.6891002163112216E-4 22-23 0.0 0.0 0.0 4.0336503244668324E-4 3.226920259573466E-4 24-25 0.0 0.0 0.0 7.52948060567142E-4 3.226920259573466E-4 26-27 0.0 0.0 0.0 0.001021858082198264 3.495830281204588E-4 28-29 0.0 0.0 0.0 0.0013176591059924985 3.76474030283571E-4 30-31 0.0 0.0 0.0 0.0021512801730489773 3.76474030283571E-4 32-33 0.0 0.0 0.0 0.003899195313651271 3.76474030283571E-4 34-35 0.0 0.0 0.0 0.005969802480210911 3.76474030283571E-4 36-37 0.0 0.0 0.0 0.009573196770067948 3.76474030283571E-4 38-39 0.0 0.0 0.0 0.014790051189711717 4.033650324466832E-4 40-41 0.0 0.0 0.0 0.02073296266775952 4.302560346097954E-4 42-43 0.0 0.0 0.0 0.027993533251799815 4.571470367729076E-4 44-45 0.0 0.0 0.0 0.03364064370605338 4.8403803893601983E-4 46-47 0.0 0.0 0.0 0.04141214333119281 4.8403803893601983E-4 48-49 0.0 0.0 0.0 0.04724749080058816 4.8403803893601983E-4 50-51 0.0 0.0 0.0 0.05362065831324576 4.8403803893601983E-4 52-53 0.0 0.0 0.0 0.06924433057001395 4.8403803893601983E-4 54-55 0.0 0.0 0.0 0.09433363558819764 4.8403803893601983E-4 56-57 0.0 0.0 0.0 0.12913059238726485 5.378200432622443E-4 58-59 0.0 0.0 0.0 0.1712687927768617 5.916020475884687E-4 60-61 0.0 0.0 0.0 0.22047932673535703 5.916020475884687E-4 62-63 0.0 0.0 0.0 0.25371660540896374 5.916020475884687E-4 64-65 0.0 0.0 0.0 0.29179426447193063 6.453840519146932E-4 66-67 0.0 0.0 0.0 0.36442686131449675 6.453840519146932E-4 68-69 0.0 0.0 0.0 0.46386978731368567 6.453840519146932E-4 70-71 0.0 0.0 0.0 0.5642001163842574 6.722750540778053E-4 72-73 0.0 0.0 0.0 0.6794549516553563 7.52948060567142E-4 74-75 0.0 0.0 0.0 0.787718126364046 8.067300648933665E-4 76-77 0.0 0.0 0.0 0.8875375263935186 8.067300648933665E-4 78-79 0.0 0.0 0.0 0.9815753609579221 8.067300648933665E-4 80-81 0.0 0.0 0.0 1.0805073579160118 8.067300648933665E-4 82-83 0.0 0.0 0.0 1.2157959897986295 8.067300648933665E-4 84-85 0.0 0.0 0.0 1.431569391155442 8.067300648933665E-4 86-87 0.0 0.0 0.0 1.7073904003424838 8.336210670564786E-4 88-89 0.0 0.0 0.0 2.0155075031274237 8.605120692195908E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14495 0.0 29.34066 1 GTACATG 12660 0.0 27.662973 1 TACATGG 12885 0.0 26.92249 2 GGTATCA 10035 0.0 26.849163 1 ACATGGG 12930 0.0 26.02025 3 ATCAACG 17620 0.0 24.05668 3 TCAACGC 17645 0.0 24.02389 4 TATCAAC 17845 0.0 23.80662 2 CAACGCA 18005 0.0 23.5674 5 AACGCAG 18290 0.0 23.277481 6 CATGGGG 5125 0.0 22.903572 4 GAGTACT 8190 0.0 22.832895 12-13 GCGCGAC 105 0.0021913513 22.628588 9 TAAGGTG 830 0.0 22.32749 5 GTGTAAG 705 0.0 22.242823 1 AGTACTT 8360 0.0 21.743292 12-13 CATGGGA 7465 0.0 21.26262 4 ATGGGAG 2810 0.0 21.137667 5 ACGCAGA 20190 0.0 20.947414 7 GTACTTT 8905 0.0 20.945667 14-15 >>END_MODULE