FastQCFastQC Report
Thu 26 May 2016
SRR936469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936469_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859358
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111500.5996693482374024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93770.5043138545670065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59440.319680233715078No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33890.18226721266157458No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22810.12267675186811793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG8050.028.9102763
GTATCAA163100.028.2620371
AGGTAAG7900.027.657072
GGTATCA115350.027.1077821
TAAGGTG9850.026.0388185
GTAAGGT9300.025.535194
GTACATG121450.024.807181
TACATGG125350.023.7584782
GAGTACT103850.022.96158212-13
TCAACGC200300.022.8348084
ACATGGG124000.022.828463
ATCAACG200450.022.8177223
TATCAAC203250.022.644812
CAACGCA204300.022.3883295
TAAGGTA4100.022.010094
AACGCAG208800.021.9974026
AGTACTT107150.021.8554312-13
GTACTTT111850.021.2977514-15
GTGTAAG7750.020.2347551
ACGCAGA231300.019.8370427
Produced by FastQC (version 0.11.5)