FastQCFastQC Report
Thu 26 May 2016
SRR936468_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936468_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914233
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93310.48745372167338047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69950.36542051046032537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43130.22531217464122708No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31360.1638254068339643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102250.029.3730431
GTATCAA147850.029.1850011
GCGCGAC908.887431E-426.4204799
GTACATG131650.025.917841
TACATGG134700.025.0820562
GAGTACT80150.023.93221312-13
ATCAACG180050.023.7593983
TATCAAC181300.023.6936382
TCAACGC180600.023.688284
ACATGGG135000.023.5547433
CAACGCA183250.023.321615
TAAGGTG8450.023.0658765
AACGCAG186350.022.9336476
TAAGGTA5200.022.8531154
AGTACTT82950.022.49396912-13
GTACTTT87600.021.59473814-15
CATGGGG49550.021.3929144
ACGCAGA206050.020.6948687
CATGGGA81850.020.442444
CGCAGAG208700.020.4157188
Produced by FastQC (version 0.11.5)