##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936468_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1914233 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.296559509735754 31.0 31.0 34.0 28.0 34.0 2 31.339781520849343 31.0 31.0 34.0 28.0 34.0 3 31.27607349784483 31.0 31.0 34.0 27.0 34.0 4 34.95712590891495 37.0 35.0 37.0 32.0 37.0 5 35.000488968688764 37.0 35.0 37.0 32.0 37.0 6 35.06817822072862 37.0 35.0 37.0 32.0 37.0 7 34.921416044964225 37.0 35.0 37.0 32.0 37.0 8 34.90591584201087 37.0 35.0 37.0 32.0 37.0 9 36.37140672008058 39.0 37.0 39.0 32.0 39.0 10-11 36.24235372600932 39.0 35.0 39.0 32.0 39.0 12-13 36.137022504574944 39.0 35.0 39.0 31.0 39.0 14-15 37.085631686424804 39.0 36.0 41.0 31.0 41.0 16-17 37.00394100404705 39.0 36.0 41.0 31.0 41.0 18-19 36.88620690375728 39.0 36.0 41.0 31.0 41.0 20-21 36.8806036151294 39.0 36.0 41.0 30.5 41.0 22-23 36.87751673908035 39.0 36.0 41.0 30.5 41.0 24-25 36.97060676521615 39.0 36.0 41.0 31.0 41.0 26-27 36.887553657261165 39.0 36.0 41.0 30.0 41.0 28-29 36.76463001108016 39.0 36.0 41.0 30.0 41.0 30-31 36.53982195479861 39.0 35.5 41.0 30.0 41.0 32-33 36.34492352811805 39.0 35.0 40.5 30.0 41.0 34-35 36.131226188243545 38.5 35.0 40.0 29.5 41.0 36-37 35.899591638008545 38.0 35.0 40.0 28.5 41.0 38-39 35.67863238174245 38.0 35.0 40.0 27.5 41.0 40-41 35.41471414399396 38.0 34.5 40.0 26.5 41.0 42-43 35.086811009944974 38.0 34.0 40.0 26.0 41.0 44-45 34.87035721356804 38.0 34.0 40.0 25.0 41.0 46-47 34.62015439081867 38.0 34.0 40.0 24.0 41.0 48-49 34.31298358141355 38.0 33.0 40.0 23.0 41.0 50-51 33.28578992212547 37.0 32.0 39.5 22.0 40.5 52-53 33.36621978620157 37.0 32.5 39.5 22.0 40.0 54-55 33.76184926286403 38.0 33.0 40.0 22.0 41.0 56-57 33.59218444149693 37.0 33.0 40.0 20.5 41.0 58-59 33.33930796303271 37.0 33.0 40.0 20.0 41.0 60-61 33.02416659831901 36.5 32.0 40.0 19.0 41.0 62-63 32.666232898502955 36.0 32.0 39.0 18.0 41.0 64-65 32.285279012534005 35.5 31.5 39.0 16.0 41.0 66-67 31.92230830834073 35.0 31.0 39.0 13.5 40.0 68-69 31.48672183584757 35.0 31.0 38.0 10.0 40.0 70-71 31.08332162281185 35.0 30.5 37.0 8.0 40.0 72-73 30.687862449346554 35.0 30.0 37.0 7.0 39.0 74-75 30.279953380805786 34.5 30.0 36.0 4.0 39.0 76-77 29.85060282630171 34.0 29.0 36.0 2.0 38.5 78-79 29.404049298073957 34.0 29.0 35.0 2.0 37.0 80-81 28.98744666924037 34.0 29.0 35.0 2.0 37.0 82-83 28.613763319303345 34.0 28.5 35.0 2.0 36.5 84-85 28.261037971866536 34.0 27.5 35.0 2.0 36.0 86-87 27.915416252880398 34.0 27.0 35.0 2.0 36.0 88-89 27.561039330112898 33.0 26.5 35.0 2.0 35.0 90-91 27.213417854566295 33.0 26.0 35.0 2.0 35.0 92-93 26.80750488576887 33.0 25.0 35.0 2.0 35.0 94-95 26.312036465780288 33.0 24.5 35.0 2.0 35.0 96-97 25.69886267763642 32.5 22.5 35.0 2.0 35.0 98-99 24.936752735952208 32.0 16.0 35.0 2.0 35.0 100-101 22.336758116697393 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4351620952224984 1101 2 0.40443780592301337 1101 3 0.5851219428011483 1101 4 0.6679300251522022 1101 5 0.5123236725423652 1101 6 0.509055385890953 1101 7 0.5521479946310279 1101 8 0.46256442514314955 1101 9 0.7216753138399383 1101 10-11 0.7088173627093042 1101 12-13 0.6820428186786032 1101 14-15 1.0229534763913648 1101 16-17 1.024376379464293 1101 18-19 1.0692910815494159 1101 20-21 0.8966752594553142 1101 22-23 0.8070152331485687 1101 24-25 0.7221532191916182 1101 26-27 0.7346140276291777 1101 28-29 0.722347938345429 1101 30-31 0.6154157239854854 1101 32-33 0.5919572443414083 1101 34-35 0.5663143792889969 1101 36-37 0.552740328424953 1101 38-39 0.4787666559617989 1101 40-41 0.6675667497203506 1101 42-43 0.7150135416527306 1101 44-45 0.5858451649650931 1101 46-47 0.6247551987972741 1101 48-49 0.6670255452497287 1101 50-51 0.49223794937522314 1101 52-53 0.6613078480886756 1101 54-55 0.4852251502344629 1101 56-57 0.7763565862296744 1101 58-59 0.6410190305338759 1101 60-61 0.5447166588560393 1101 62-63 0.6705512271790326 1101 64-65 0.695640672616868 1101 66-67 0.5650347581665578 1101 68-69 0.6966878071915836 1101 70-71 0.4676059021516359 1101 72-73 0.738767261110354 1101 74-75 0.5374718739565054 1101 76-77 0.509363243417841 1101 78-79 0.6024504744248773 1101 80-81 0.6740897235251957 1101 82-83 0.7269484770984285 1101 84-85 0.7587441712629435 1101 86-87 0.5522141601702337 1101 88-89 0.6720592207833818 1101 90-91 0.5489128937907424 1101 92-93 0.6506113743172648 1101 94-95 1.0015568723214834 1101 96-97 0.594910224265405 1101 98-99 0.4872667217454847 1101 100-101 1.058120004085012 1102 1 0.2255200101413024 1102 2 0.4368365157221916 1102 3 0.4330333015438903 1102 4 0.3492894541697069 1102 5 0.3092524219740369 1102 6 0.37547853144173615 1102 7 0.4762291340224678 1102 8 0.4283344815894381 1102 9 0.46124497524800745 1102 10-11 0.5578021016237855 1102 12-13 0.48327031629808204 1102 14-15 0.7282796563589429 1102 16-17 0.39602876835175493 1102 18-19 0.5573880621449376 1102 20-21 0.5494919977521491 1102 22-23 0.44571155341689916 1102 24-25 0.5038161917576858 1102 26-27 0.4632178667057971 1102 28-29 0.3816045555572529 1102 30-31 0.2831467902455316 1102 32-33 0.3755001066313355 1102 34-35 0.10860365470912114 1102 36-37 0.4099065082638518 1102 38-39 0.24707577658356428 1102 40-41 0.2992158627072925 1102 42-43 0.3109206229594008 1102 44-45 0.3745967627038027 1102 46-47 0.45104306734948807 1102 48-49 0.5586363982920801 1102 50-51 0.5593441818429881 1102 52-53 0.30007051740609114 1102 54-55 0.3108016765574817 1102 56-57 0.25503843570338347 1102 58-59 0.13171501731446966 1102 60-61 0.2734994393074359 1102 62-63 0.2654975206396699 1102 64-65 0.34018455574388895 1102 66-67 0.0866311725835196 1102 68-69 0.3440711615421499 1102 70-71 -0.14615537316555205 1102 72-73 0.12107745189537056 1102 74-75 0.0344202198143968 1102 76-77 0.010513427640333362 1102 78-79 -0.11421978447313208 1102 80-81 0.23828651668129552 1102 82-83 0.11299163963456138 1102 84-85 0.056012708248989185 1102 86-87 -0.06112757469521313 1102 88-89 -0.09632094023436721 1102 90-91 0.13193204091995625 1102 92-93 0.2620154488726847 1102 94-95 0.08926173673888016 1102 96-97 0.33029011077234216 1102 98-99 0.4202611521784405 1102 100-101 0.9443579245369271 1103 1 0.387324288429987 1103 2 0.35132272123998476 1103 3 0.42275541269942707 1103 4 0.447774060327248 1103 5 0.3919558406065846 1103 6 0.3719329496744379 1103 7 0.46157199687732486 1103 8 0.23971442961023115 1103 9 0.3719187057558102 1103 10-11 0.2812127373694864 1103 12-13 0.301947095586371 1103 14-15 0.4811657519207131 1103 16-17 0.5759730406428432 1103 18-19 0.7162497674628057 1103 20-21 0.6578526534898543 1103 22-23 0.43299613958243555 1103 24-25 0.34157083989843073 1103 26-27 0.3952163173255414 1103 28-29 0.44305322608904163 1103 30-31 0.38568502495164836 1103 32-33 0.5116076636085509 1103 34-35 0.5160379309986141 1103 36-37 0.4990423539255815 1103 38-39 0.27167893532007525 1103 40-41 0.5009586655861327 1103 42-43 0.6113701931313358 1103 44-45 0.6505553792571845 1103 46-47 0.7287532332831148 1103 48-49 0.7949373779002329 1103 50-51 0.777637114669858 1103 52-53 0.7641061531517934 1103 54-55 0.6841368424911067 1103 56-57 0.7867704928805139 1103 58-59 0.7517157670145878 1103 60-61 0.7050578159567777 1103 62-63 0.839213784134273 1103 64-65 0.8907543278350474 1103 66-67 0.7846981957742436 1103 68-69 0.8091831167600638 1103 70-71 0.7287764041235327 1103 72-73 0.8185109784847349 1103 74-75 0.642183364556498 1103 76-77 0.6558305507910731 1103 78-79 0.46463717274398064 1103 80-81 0.5768248513474283 1103 82-83 0.617344898331087 1103 84-85 0.7394315906960109 1103 86-87 0.5745783929177399 1103 88-89 0.4238135059871446 1103 90-91 0.7624098497964056 1103 92-93 0.6733126799802456 1103 94-95 0.9227738319008161 1103 96-97 0.8622648208882957 1103 98-99 1.1654405804071502 1103 100-101 1.6236424607224507 1104 1 0.3527347011879627 1104 2 0.2896974867184099 1104 3 0.42616066598272795 1104 4 0.481934473085218 1104 5 0.3666767599311669 1104 6 0.39619889714160905 1104 7 0.3050358805546267 1104 8 0.4827796266083553 1104 9 0.5857484430916458 1104 10-11 0.52625260603741 1104 12-13 0.4834949379878708 1104 14-15 0.6171155643034538 1104 16-17 0.6188377216934953 1104 18-19 0.630118200858675 1104 20-21 0.7473718842590884 1104 22-23 0.40878295946986754 1104 24-25 0.6204263746076251 1104 26-27 0.22378690831991577 1104 28-29 0.07791603096708855 1104 30-31 0.12765735140568069 1104 32-33 0.2445180763665178 1104 34-35 0.30926963831568344 1104 36-37 0.3498655246572966 1104 38-39 0.2973989165583433 1104 40-41 0.4421383091133393 1104 42-43 0.6065542925684753 1104 44-45 0.5586604449232269 1104 46-47 0.933672323339394 1104 48-49 0.8297626593261285 1104 50-51 0.7216826118555986 1104 52-53 0.7177998679735609 1104 54-55 0.8129020863935423 1104 56-57 0.9633218531056542 1104 58-59 0.935316845813837 1104 60-61 0.7272470748685933 1104 62-63 0.5969248535526503 1104 64-65 0.8059395998800838 1104 66-67 0.7385384865810032 1104 68-69 0.6591878071915822 1104 70-71 0.6465109256995163 1104 72-73 0.732805068541019 1104 74-75 0.5948158692469327 1104 76-77 0.4833657290274722 1104 78-79 0.4228782609240902 1104 80-81 0.6941266806157209 1104 82-83 0.5030097669990035 1104 84-85 0.31648717230951817 1104 86-87 0.3533506724674602 1104 88-89 0.5590620988576873 1104 90-91 0.8090679173386235 1104 92-93 0.8420556443329623 1104 94-95 0.8202398262722959 1104 96-97 0.658852531957713 1104 98-99 0.5609846704634336 1104 100-101 1.1393847215104458 1105 1 0.33087132342163983 1105 2 0.2932879514236433 1105 3 0.37493722451275957 1105 4 0.40984670565877224 1105 5 0.5036099171282018 1105 6 0.3196185035046071 1105 7 0.3305171100718738 1105 8 0.2610396056192883 1105 9 0.14329046724747485 1105 10-11 0.4546152289338963 1105 12-13 0.3703556000639452 1105 14-15 0.5501261600982801 1105 16-17 0.595794054314922 1105 18-19 0.4531335606185962 1105 20-21 0.5403849656009143 1105 22-23 0.4706349911743928 1105 24-25 0.36310045495523724 1105 26-27 0.30768873381610007 1105 28-29 0.3847569237387489 1105 30-31 0.2991092330568179 1105 32-33 0.364139858964009 1105 34-35 0.19906738843535976 1105 36-37 0.3198541555811758 1105 38-39 0.03453045722925907 1105 40-41 0.2554343745844889 1105 42-43 0.19984688287668462 1105 44-45 0.14599151978422498 1105 46-47 0.5982128883870814 1105 48-49 0.757272256299288 1105 50-51 0.6124140531046294 1105 52-53 0.6374129051278814 1105 54-55 0.35296461631217113 1105 56-57 0.3193913057329709 1105 58-59 0.3330500499601001 1105 60-61 0.27681452064685175 1105 62-63 0.5464185384221167 1105 64-65 0.6391559437324972 1105 66-67 0.48789788286925173 1105 68-69 0.563186315863387 1105 70-71 0.6315069672357687 1105 72-73 0.3996269284666347 1105 74-75 0.349468329938464 1105 76-77 0.5472403746068792 1105 78-79 0.6385643639546181 1105 80-81 0.8462523986626351 1105 82-83 0.7075496876456135 1105 84-85 0.5860128701038256 1105 86-87 0.7183075666383907 1105 88-89 0.7910764045615579 1105 90-91 0.6873324992143814 1105 92-93 0.7747636017656703 1105 94-95 0.9700921893830987 1105 96-97 0.9886761041490431 1105 98-99 1.2779591153579375 1105 100-101 1.2837990299760342 1106 1 0.17607430308054361 1106 2 0.08373349936026031 1106 3 0.16490775503015342 1106 4 0.26501999040307567 1106 5 0.25935941065382195 1106 6 0.18599138780808033 1106 7 0.28571986199845867 1106 8 0.46915290313960867 1106 9 0.47285962299775264 1106 10-11 0.5453593132417822 1106 12-13 0.5706673403092353 1106 14-15 0.4243428126677742 1106 16-17 0.3638781284875705 1106 18-19 0.40836472157727144 1106 20-21 0.27663689183680873 1106 22-23 0.12081824668384655 1106 24-25 -0.015114970160048813 1106 26-27 0.09688589456913377 1106 28-29 0.12580917128423863 1106 30-31 0.2878454543887159 1106 32-33 0.25409626547678954 1106 34-35 0.2422766061196242 1106 36-37 -0.14880999192127575 1106 38-39 0.3661355169353797 1106 40-41 0.12073703199065022 1106 42-43 0.1398656397388507 1106 44-45 0.012098141297016696 1106 46-47 0.22726649496546258 1106 48-49 0.26195698620299623 1106 50-51 0.13976355481941027 1106 52-53 0.11325993709713345 1106 54-55 0.3234574083541375 1106 56-57 0.4275126313927231 1106 58-59 0.28647625974205226 1106 60-61 0.45684002666691725 1106 62-63 0.37456873777991007 1106 64-65 0.08895465366340005 1106 66-67 0.30239196401418766 1106 68-69 0.48830056732909455 1106 70-71 0.19975224828850813 1106 72-73 0.24126739249769713 1106 74-75 0.3997957929152882 1106 76-77 0.33017731184638066 1106 78-79 0.46287336313087124 1106 80-81 0.6056430789818918 1106 82-83 0.5772146841985766 1106 84-85 0.7484664121350448 1106 86-87 0.8234449087813651 1106 88-89 0.7151931194469014 1106 90-91 1.0130667806809512 1106 92-93 1.0474284310848958 1106 94-95 1.2533938433868492 1106 96-97 1.1486011273075825 1106 98-99 1.0477837315657155 1106 100-101 1.6821890084656808 1107 1 0.11750220979875436 1107 2 0.15391964515698575 1107 3 0.19355446621235117 1107 4 0.21069451900944358 1107 5 0.20345201825492865 1107 6 0.1577301830199076 1107 7 0.21054678755806577 1107 8 0.3424524165050258 1107 9 0.2905130814383696 1107 10-11 0.3285882834197267 1107 12-13 0.24047685532426755 1107 14-15 0.02628247015879026 1107 16-17 0.059118290005777396 1107 18-19 0.37637968191493343 1107 20-21 0.16207839975851357 1107 22-23 0.1577137861059157 1107 24-25 0.30842149515871853 1107 26-27 0.09473624815917958 1107 28-29 0.1546884190267903 1107 30-31 0.04556421133679578 1107 32-33 0.17113259990269114 1107 34-35 0.18921366816642404 1107 36-37 0.14389537540355946 1107 38-39 0.38467288326328486 1107 40-41 0.24927091531311163 1107 42-43 -4.7067872887396334E-4 1107 44-45 0.028411449515651555 1107 46-47 0.010186387633311256 1107 48-49 -0.004253557665833796 1107 50-51 -0.07253739388492875 1107 52-53 0.1981887887542726 1107 54-55 0.15096178788608228 1107 56-57 0.3102363192365374 1107 58-59 0.4182064841605637 1107 60-61 0.2300958119524097 1107 62-63 0.2693703185354366 1107 64-65 0.3045762244495407 1107 66-67 0.12625375638582526 1107 68-69 0.25832672797229606 1107 70-71 0.25583569607838896 1107 72-73 0.2827333119394133 1107 74-75 0.26176836637666767 1107 76-77 0.2451445751813175 1107 78-79 0.38496207263476734 1107 80-81 0.35872556121273647 1107 82-83 0.23944059363505588 1107 84-85 0.2526919656505058 1107 86-87 0.6467418894594239 1107 88-89 0.5698614673995941 1107 90-91 0.37804395063368545 1107 92-93 0.5738043527141343 1107 94-95 0.45721959091064335 1107 96-97 0.9084686341391119 1107 98-99 1.045987540727495 1107 100-101 1.3095555022222705 1108 1 0.15430804387214891 1108 2 0.11721944892127212 1108 3 0.2722886742508521 1108 4 0.27627413054867844 1108 5 0.24464719400764068 1108 6 0.3262529721452978 1108 7 0.31041662025638317 1108 8 0.32806328968231924 1108 9 -0.015828590719074498 1108 10-11 0.1419168242568034 1108 12-13 -0.006369228555762163 1108 14-15 0.47261442005422793 1108 16-17 0.4814655269496626 1108 18-19 0.45191476441988243 1108 20-21 0.4400791410138467 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0.32424956852982945 2311 7 0.6022809884270615 2311 8 0.2559565926704934 2311 9 0.5699147856633857 2311 10-11 0.32908783959043575 2311 12-13 0.5348203156437492 2311 14-15 0.8159869463806686 2311 16-17 0.7222190978346461 2311 18-19 0.7782113713257814 2311 20-21 0.5845210941497854 2311 22-23 0.5802401328845335 2311 24-25 0.6461683814033847 2311 26-27 0.5924845840475896 2311 28-29 0.5950577952033811 2311 30-31 0.5528090790021949 2311 32-33 0.4928552135507829 2311 34-35 0.5186429751970039 2311 36-37 0.5590160036354703 2311 38-39 0.6221919222487671 2311 40-41 0.6185872692803684 2311 42-43 0.6129387493171592 2311 44-45 0.7308940388971124 2311 46-47 0.7767464574942267 2311 48-49 0.4733628507127108 2311 50-51 0.6057619605271469 2311 52-53 0.7290115906574712 2311 54-55 0.8503738133759882 2311 56-57 0.7365081582188466 2311 58-59 0.8066256178693507 2311 60-61 0.686071198788845 2311 62-63 0.8030655994485869 2311 64-65 0.7661207007111344 2311 66-67 0.7655760628285968 2311 68-69 0.8187863047557578 2311 70-71 0.7996063733598895 2311 72-73 0.5890427870492267 2311 74-75 0.6076910944076168 2311 76-77 0.8826761744362415 2311 78-79 0.9424677520609492 2311 80-81 0.9285648791255703 2311 82-83 0.7361087711747096 2311 84-85 0.6636354355018845 2311 86-87 0.7599567077972189 2311 88-89 0.8354647475660855 2311 90-91 0.6941937514566057 2311 92-93 0.9721596276301376 2311 94-95 0.9601571053077897 2311 96-97 0.978686373608344 2311 98-99 0.9903098024894703 2311 100-101 0.6847705906306807 2312 1 0.3118466679150238 2312 2 0.2782115288860929 2312 3 0.12341400151229465 2312 4 0.2900694590842292 2312 5 0.23681038701072055 2312 6 0.23756255222314593 2312 7 0.31450404889591965 2312 8 0.3273882166264741 2312 9 0.47728299258925233 2312 10-11 0.3989270007005601 2312 12-13 0.1743284085858008 2312 14-15 0.43962112351939453 2312 16-17 0.40284931368822896 2312 18-19 0.3104854179563574 2312 20-21 0.29747792115581007 2312 22-23 0.3086589065955394 2312 24-25 0.1881705683154209 2312 26-27 0.3089978496804804 2312 28-29 -0.07417845511428567 2312 30-31 0.30618745353053356 2312 32-33 0.4312799360272095 2312 34-35 0.023910965938803486 2312 36-37 0.3092739829014022 2312 38-39 0.3832321812593804 2312 40-41 0.49259025277995505 2312 42-43 0.1432065121369206 2312 44-45 0.1542089542297731 2312 46-47 0.3654025778279504 2312 48-49 0.24971149394975356 2312 50-51 0.31397074395903957 2312 52-53 0.15441142290684695 2312 54-55 -0.2894600656407178 2312 56-57 0.18094817084573123 2312 58-59 0.38943838674613573 2312 60-61 0.04658233676943979 2312 62-63 0.04381581056138373 2312 64-65 0.1791637392310932 2312 66-67 0.17142588157359384 2312 68-69 0.16462966191074102 2312 70-71 0.2979666166894681 2312 72-73 0.24065282838288837 2312 74-75 -0.16300152080413355 2312 76-77 -0.07689102439041662 2312 78-79 0.15000929370287608 2312 80-81 -0.15438108169691134 2312 82-83 -0.24090443576825038 2312 84-85 -0.4574128235725432 2312 86-87 -0.5082202178306794 2312 88-89 -0.2196502220120209 2312 90-91 -0.18589275635599378 2312 92-93 -0.021554139887097534 2312 94-95 -0.11039402729383951 2312 96-97 -0.05360320021161513 2312 98-99 -0.04266382237135602 2312 100-101 -0.23162860001813357 2313 1 -0.10437530206116463 2313 2 -0.2088998043221224 2313 3 -0.056880922840274906 2313 4 0.05385694079701864 2313 5 -0.09272286235784577 2313 6 0.07052825706362853 2313 7 0.19551847741922046 2313 8 0.1424635474921132 2313 9 0.023670321899210478 2313 10-11 -0.03660733629059365 2313 12-13 -0.13185515542452464 2313 14-15 -0.05090033611080713 2313 16-17 -0.06453614111212147 2313 18-19 -0.04716151849799388 2313 20-21 -0.056476807967378306 2313 22-23 -0.13432711485391025 2313 24-25 -0.18688822209450962 2313 26-27 -0.271689444129116 2313 28-29 -0.1421506268998627 2313 30-31 -0.13362473372144024 2313 32-33 -0.2614963619406794 2313 34-35 -0.5382884398258128 2313 36-37 -0.43189925886963465 2313 38-39 -0.44051077802373584 2313 40-41 -0.19671769364841651 2313 42-43 -0.40595357641048224 2313 44-45 -0.3176940348103443 2313 46-47 -0.44400541404992566 2313 48-49 -0.4884682419004136 2313 50-51 -0.7252119336727958 2313 52-53 -0.5140623893572851 2313 54-55 -0.6958657330362357 2313 56-57 -0.5432609285530248 2313 58-59 -0.5889015868886247 2313 60-61 -0.6395034429609865 2313 62-63 -0.4965322173595368 2313 64-65 -0.666607817064115 2313 66-67 -0.6455745525709773 2313 68-69 -0.7866198038879375 2313 70-71 -0.7594583294381643 2313 72-73 -0.635187478774391 2313 74-75 -0.5302417519861145 2313 76-77 -0.5343069823688396 2313 78-79 -0.5767227056911288 2313 80-81 -0.41516912191859845 2313 82-83 -0.471752894441984 2313 84-85 -0.6127506536185159 2313 86-87 -0.6231517948382042 2313 88-89 -0.4467507920528817 2313 90-91 -0.41480961088817025 2313 92-93 -0.1752743857214618 2313 94-95 -0.34221676742439655 2313 96-97 -0.4243205449653651 2313 98-99 -0.25626210071681044 2313 100-101 -0.20464069275322316 2314 1 -0.6571644160882038 2314 2 -0.6266880744417413 2314 3 -0.4942207968167658 2314 4 -0.22191956222373221 2314 5 -0.22865741409153628 2314 6 -0.11427556691891994 2314 7 -0.3260249643381883 2314 8 -0.307321256115479 2314 9 -0.37482059852248284 2314 10-11 -0.3428126524745707 2314 12-13 -0.8397317328822425 2314 14-15 -1.05168960593614 2314 16-17 -1.135221231648245 2314 18-19 -1.1964541509698208 2314 20-21 -1.303735461264246 2314 22-23 -1.161614266255235 2314 24-25 -1.0238477426685435 2314 26-27 -0.9645485267368272 2314 28-29 -0.829717165754225 2314 30-31 -1.0260232917216783 2314 32-33 -1.1229630081227526 2314 34-35 -1.3814065911925155 2314 36-37 -1.3710873441951676 2314 38-39 -1.00881561685906 2314 40-41 -0.7883865963091026 2314 42-43 -0.7800117540897844 2314 44-45 -0.8269913520502854 2314 46-47 -0.9295921785521557 2314 48-49 -1.3921832738390663 2314 50-51 -1.0600205722553753 2314 52-53 -1.15356677767587 2314 54-55 -1.0882761922949769 2314 56-57 -1.2298488593785848 2314 58-59 -0.9005595728494669 2314 60-61 -1.0653837574138763 2314 62-63 -1.1881397744422983 2314 64-65 -1.0040777393380793 2314 66-67 -1.0406741552853305 2314 68-69 -0.917199330261127 2314 70-71 -1.033450455132769 2314 72-73 -0.9265659907275818 2314 74-75 -0.9094834687101958 2314 76-77 -0.7991689494088483 2314 78-79 -0.6572525713583808 2314 80-81 -0.5526970898760801 2314 82-83 -0.6124181964310118 2314 84-85 -0.6975733573247815 2314 86-87 -0.6722956074820985 2314 88-89 -0.7087697107261732 2314 90-91 -0.7233445984243048 2314 92-93 -0.6785583216191178 2314 94-95 -0.8579718698184955 2314 96-97 -1.0173021023424162 2314 98-99 -0.815138437985496 2314 100-101 -1.386072403331923 2315 1 -0.885565930735897 2315 2 -1.3046259256704182 2315 3 -1.1985506774405827 2315 4 -0.8197409052889384 2315 5 -0.9617412850056439 2315 6 -0.622707133440386 2315 7 -0.4042762196170102 2315 8 -0.3617544270661668 2315 9 -0.7025190323335408 2315 10-11 -1.039008160072754 2315 12-13 -1.0349244722165238 2315 14-15 -0.7407493522201847 2315 16-17 -1.0735260441046037 2315 18-19 -1.2701602162110674 2315 20-21 -1.1280094154756597 2315 22-23 -1.052934449642322 2315 24-25 -1.3320857177624816 2315 26-27 -1.3468271695393454 2315 28-29 -1.1456577519943707 2315 30-31 -1.072624478833042 2315 32-33 -1.1462926975451637 2315 34-35 -0.9161106862279311 2315 36-37 -1.1900305199674577 2315 38-39 -1.393480120732967 2315 40-41 -1.6312418984073318 2315 42-43 -1.5337391424447802 2315 44-45 -1.7659923870318295 2315 46-47 -2.1594832976623195 2315 48-49 -2.568214306659204 2315 50-51 -2.29802112507684 2315 52-53 -2.0344108903350744 2315 54-55 -2.048805570807268 2315 56-57 -2.4387821217734214 2315 58-59 -2.537236887217837 2315 60-61 -2.617102180085375 2315 62-63 -2.756476613134815 2315 64-65 -2.2245204952985773 2315 66-67 -2.1014419120771386 2315 68-69 -2.2418946549709986 2315 70-71 -1.8960249613343443 2315 72-73 -1.9408208871462982 2315 74-75 -2.0561110499292248 2315 76-77 -1.9792152118369408 2315 78-79 -1.9307318966667957 2315 80-81 -2.247297000925858 2315 82-83 -2.008099122843408 2315 84-85 -2.0247756026943833 2315 86-87 -2.0727342276729672 2315 88-89 -2.3877607284164597 2315 90-91 -2.226599698514086 2315 92-93 -2.094619343964741 2315 94-95 -2.197508164833959 2315 96-97 -2.043742451659078 2315 98-99 -2.280858830862826 2315 100-101 -2.7980787088218975 2316 1 -5.263728875557874 2316 2 -5.3276966111192365 2316 3 -6.292414307430953 2316 4 -8.387726108268911 2316 5 -8.997017408803327 2316 6 -9.73975244989845 2316 7 -11.804041131143354 2316 8 -11.831805183742588 2316 9 -11.917107370449656 2316 10-11 -11.386364816862276 2316 12-13 -9.704053803593126 2316 14-15 -10.078406856823808 2316 16-17 -8.958412145374922 2316 18-19 -9.72078462615302 2316 20-21 -10.50076485803088 2316 22-23 -10.236913380480509 2316 24-25 -9.956953100755722 2316 26-27 -8.006128237018615 2316 28-29 -6.26769168792692 2316 30-31 -8.631499419243024 2316 32-33 -6.073846422038297 2316 34-35 -4.83278105292856 2316 36-37 -5.299990466126118 2316 38-39 -4.395030629701846 2316 40-41 -3.9904055583095577 2316 42-43 -3.9550207251557303 2316 44-45 -4.228046396622322 2316 46-47 -4.133146845395096 2316 48-49 -4.968543570734134 2316 50-51 -5.276932684138728 2316 52-53 -4.88106356697859 2316 54-55 -4.252290885956686 2316 56-57 -4.93182060524245 2316 58-59 -5.106666271874925 2316 60-61 -5.316145117616337 2316 62-63 -5.016705064915975 2316 64-65 -5.776311818056822 2316 66-67 -5.232893408137183 2316 68-69 -5.503817332829687 2316 70-71 -5.163135476285596 2316 72-73 -4.079621819087482 2316 74-75 -4.581536709625812 2316 76-77 -4.946027659841725 2316 78-79 -5.2056089776437915 2316 80-81 -5.863347397725292 2316 82-83 -5.416419291845376 2316 84-85 -5.188631801458651 2316 86-87 -5.872479042172738 2316 88-89 -6.9571112439285585 2316 90-91 -6.361405762207639 2316 92-93 -5.7904239728240725 2316 94-95 -5.400017839449081 2316 96-97 -6.4666592546427815 2316 98-99 -8.136839682602854 2316 100-101 -8.678327304366903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4493.0 3 1863.0 4 1950.0 5 2151.0 6 2439.0 7 2895.0 8 3551.0 9 4554.0 10 5860.0 11 7260.0 12 9277.0 13 11164.0 14 12567.0 15 15343.0 16 19275.0 17 23999.0 18 18969.0 19 14106.0 20 14203.0 21 14995.0 22 16671.0 23 19087.0 24 21734.0 25 24587.0 26 28503.0 27 33912.0 28 40640.0 29 48094.0 30 58301.0 31 70770.0 32 87247.0 33 111240.0 34 145289.0 35 196172.0 36 262578.0 37 315415.0 38 215537.0 39 27542.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.33352572537813 18.199503231193024 14.134227956341643 22.332743087087202 2 16.01752051846688 20.278714618512765 39.06765215228345 24.63611271073691 3 19.435235648516063 25.204565890828874 29.37869756015238 25.981500900502684 4 12.873236978604693 16.302588891077637 36.42124095596738 34.402933174350295 5 14.06452401766805 37.76877387018175 32.563617675783036 15.60308443636717 6 32.06732070612851 35.317869658233555 17.26155203208953 15.353257603548409 7 29.288354893504447 33.305432242960116 19.717531522045434 17.68868134149 8 27.966288622828355 32.46099952853414 19.414985087670697 20.157726760966806 9 28.13747826514278 16.16278741506023 19.79584133632646 35.903892983470534 10-11 25.847010317361786 26.0378063681449 26.094391772247015 22.020791542246297 12-13 27.270095148900765 23.710843713514322 26.7810498677239 22.238011269861012 14-15 23.92265788204206 24.655328396424476 25.8044689321256 25.61754478940786 16-17 22.784202399632854 28.124232277434942 26.08696447594147 23.004600846990737 18-19 22.695542388043553 25.916959999958184 29.20366054859097 22.183837063407292 20-21 23.975183384111535 24.902645360547872 28.884088283533078 22.23808297180752 22-23 23.288088136831213 25.099890098034017 28.94682463778982 22.665197127344953 24-25 23.22688184263363 25.40978402718177 29.059519564004795 22.303814566179803 26-27 22.7754302881808 25.686108374332754 29.051731773252097 22.48672956423434 28-29 22.462602860744667 25.85832005044624 29.2039358095665 22.475141279242596 30-31 22.497238417613946 25.43218209282626 29.157947885041903 22.912631604517895 32-33 22.40346870306858 25.704227651663675 28.87061040595247 23.021693239315276 34-35 23.314176445387197 25.578703461823803 28.529978788100436 22.57714130468856 36-37 23.46840634224068 25.13394979494815 28.610688817490793 22.78695504532038 38-39 22.95418864237779 25.418158925208132 28.62197597235175 23.00567646006233 40-41 22.967171581948016 25.560176987220697 28.70893835617333 22.76371307465796 42-43 23.058262824759115 25.720122579391365 28.058685980687372 23.162928615162148 44-45 22.871625451424315 25.833799580925692 28.08009428388288 23.214480683767114 46-47 23.369449805626147 26.124143810793253 27.487359757102553 23.019046626478044 48-49 23.500975858473357 26.241111224880004 27.017670978972024 23.240241937674615 50-51 23.30340894576284 26.53403081735658 26.96684565227442 23.195714584606158 52-53 23.72436339301876 26.484793759163278 26.72073713170906 23.070105716108902 54-55 22.99666575681313 26.373040821950365 26.927180807923822 23.70311261331268 56-57 23.372096273214858 26.677755972408733 26.571547310786762 23.378600443589644 58-59 23.656290349165335 26.009344615274504 26.69056064295885 23.643804392601314 60-61 23.252594920705242 26.204415178953923 27.364606472725907 23.17838342761493 62-63 23.271114138817612 26.324311772156495 27.16963744404569 23.2349366449802 64-65 23.040575732294514 26.510338659602358 26.99471273723072 23.45437287087241 66-67 23.02529538094569 26.806124469301647 26.62516331691762 23.543416832835046 68-69 23.1363065497274 26.448419042126137 27.058583521928202 23.35669088621826 70-71 23.17035932609281 26.503926876279433 26.835400318830953 23.4903134787968 72-73 23.223931288984684 26.537108520296528 26.747578459227327 23.491381731491458 74-75 23.013087157322293 27.186143612310403 26.442515940290335 23.35825329007697 76-77 23.210750610919238 26.81050386564976 26.51220963210552 23.46653589132548 78-79 23.203969404941954 26.886124403913914 26.613374116990542 23.296532074153593 80-81 23.223825948070004 27.308611555864857 26.35097379518757 23.116588700877568 82-83 23.262047241544053 27.116124905081467 26.280267158932606 23.341560694441867 84-85 23.073280173194053 27.088527644204085 26.14999267300141 23.68819950960046 86-87 23.367571389651683 26.87220296986384 26.08790481432017 23.67232082616431 88-89 23.242698381934165 26.97402412183895 25.95798555949512 23.82529193673176 90-91 22.97666744858046 26.940265094898034 26.39191539448944 23.69115206203206 92-93 23.19009811551032 26.778410564782646 26.49291190606992 23.538579413637112 94-95 22.885001965811856 26.592562308398655 26.5975519438026 23.924883781986892 96-97 23.068790643203968 26.434223612106344 26.558231671715898 23.938754072973794 98-99 22.883153561878437 26.78602080445821 26.084709650702997 24.246115982960355 100-101 22.874997028223646 26.570502160971266 26.42790914698393 24.126591663821166 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 706.0 1 512.0 2 445.0 3 597.0 4 579.5 5 593.0 6 865.0 7 1571.5 8 2474.0 9 2877.5 10 2546.5 11 2190.0 12 2281.0 13 2468.0 14 2682.0 15 2837.0 16 2936.0 17 3104.0 18 3193.0 19 3372.0 20 3712.5 21 4089.5 22 4760.0 23 5608.5 24 6425.0 25 7730.0 26 9429.5 27 11453.5 28 13511.0 29 15618.0 30 18443.5 31 21950.0 32 26972.0 33 29905.0 34 31190.5 35 33267.5 36 35892.5 37 39406.0 38 41298.0 39 41534.0 40 43035.0 41 47860.0 42 52530.0 43 55847.0 44 58596.5 45 62157.5 46 64298.0 47 66902.0 48 76962.0 49 78685.0 50 71133.0 51 67288.5 52 66670.0 53 68841.0 54 69390.0 55 66737.5 56 62708.0 57 57513.0 58 54288.0 59 50688.5 60 44450.5 61 38659.5 62 33744.5 63 26540.0 64 19389.0 65 15617.0 66 12072.5 67 9592.5 68 7228.0 69 5014.5 70 4009.0 71 3166.0 72 2604.0 73 2389.0 74 1925.0 75 1587.5 76 1191.0 77 735.0 78 493.5 79 343.5 80 233.5 81 156.0 82 102.5 83 63.0 84 30.5 85 17.5 86 19.5 87 14.0 88 9.0 89 7.0 90 6.0 91 3.0 92 2.5 93 2.0 94 2.5 95 3.0 96 2.0 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07689763994247305 2 0.030873984514946717 3 0.015776553846893245 4 0.021000578299506904 5 0.025911161284963742 6 0.030665023536842168 7 0.07961413265783214 8 0.054904496996969546 9 0.07350202404827416 10-11 0.06245321233099629 12-13 0.042366838310696764 14-15 0.046493817628261554 16-17 0.05782995069043319 18-19 0.053415650027974645 20-21 0.018493046562252348 22-23 0.01329514223190176 24-25 0.023534230159024526 26-27 0.025414878961965443 28-29 0.006059868365031843 30-31 0.001175405501838073 32-33 0.010630889761068794 34-35 0.011127172084067091 36-37 0.004335940295669336 38-39 0.009821165970913676 40-41 0.004362060417932404 42-43 0.002350811003676146 44-45 7.313634233659121E-4 46-47 4.962823229982975E-4 48-49 0.0010970451350488682 50-51 0.0010186847682596633 52-53 1.3060061131534145E-4 54-55 2.8732134489375114E-4 56-57 0.003996378706249448 58-59 0.0043098201734062674 60-61 0.005694186653348887 62-63 0.0031082945493051263 64-65 5.224024452613658E-4 66-67 5.224024452613658E-4 68-69 0.004022498828512517 70-71 0.00274261283762217 72-73 0.021967022823240433 74-75 0.019433370963722805 76-77 0.027086566786801818 78-79 0.02060877646556088 80-81 0.013190661742849486 82-83 0.004779982374141497 84-85 0.0041530994398278585 86-87 0.0019590091697301217 88-89 0.0017761683138886437 90-91 0.00658227081029321 92-93 0.01115329220633016 94-95 0.013869784921689263 96-97 0.012772739786640395 98-99 0.024056632604285894 100-101 0.02060877646556088 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1914233.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.80995407923891 #Duplication Level Percentage of deduplicated Percentage of total 1 71.64141131050074 32.102483509954055 2 14.367830146393345 12.876436181563808 3 5.450308622834775 7.3268423732071835 4 2.7051068485445144 4.848628546508575 5 1.5388559858257633 3.447803302970719 6 0.9390124356644429 2.524626247317478 7 0.6370394028682258 1.9981994472433686 8 0.45812382039146504 1.6422805883477634 9 0.3242524429565673 1.3076763371068334 >10 1.6704483907587289 13.750668087431766 >50 0.1532380028687286 4.7687311068391045 >100 0.10484228907781377 8.684797635643458 >500 0.006353534209990066 1.9100839636678986 >1k 0.002941451023143549 1.949994906848682 >5k 2.3531608185148396E-4 0.860747765349364 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9331 0.48745372167338047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6995 0.36542051046032537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4313 0.22531217464122708 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3136 0.1638254068339643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 5.224024452613658E-5 12-13 0.0 0.0 0.0 0.0 1.0448048905227315E-4 14-15 0.0 0.0 0.0 0.0 1.8284085584147801E-4 16-17 0.0 0.0 0.0 5.224024452613658E-5 2.612012226306829E-4 18-19 0.0 0.0 0.0 5.224024452613658E-5 3.918018339460243E-4 20-21 0.0 0.0 0.0 1.3060061131534145E-4 4.179219562090926E-4 22-23 0.0 0.0 0.0 2.089609781045463E-4 4.179219562090926E-4 24-25 0.0 0.0 0.0 3.134414671568195E-4 4.179219562090926E-4 26-27 0.0 0.0 0.0 4.962823229982975E-4 4.179219562090926E-4 28-29 0.0 0.0 0.0 0.0010970451350488682 4.179219562090926E-4 30-31 0.0 0.0 0.0 0.002585892104043761 4.179219562090926E-4 32-33 0.0 0.0 0.0 0.0042837000511432 4.179219562090926E-4 34-35 0.0 0.0 0.0 0.006294949465399458 4.179219562090926E-4 36-37 0.0 0.0 0.0 0.009847286093176744 4.179219562090926E-4 38-39 0.0 0.0 0.0 0.015019070301264266 4.440420784721609E-4 40-41 0.0 0.0 0.0 0.020556536221034743 4.701622007352292E-4 42-43 0.0 0.0 0.0 0.026459683852488175 4.962823229982975E-4 44-45 0.0 0.0 0.0 0.03129190647115581 5.485225675244341E-4 46-47 0.0 0.0 0.0 0.03993766694023142 5.746426897875024E-4 48-49 0.0 0.0 0.0 0.04607589567205246 5.746426897875024E-4 50-51 0.0 0.0 0.0 0.05325892929439624 5.746426897875024E-4 52-53 0.0 0.0 0.0 0.06825187947339745 5.746426897875024E-4 54-55 0.0 0.0 0.0 0.09290927488973391 5.746426897875024E-4 56-57 0.0 0.0 0.0 0.12723111554340563 5.746426897875024E-4 58-59 0.0 0.0 0.0 0.16897107091978875 5.746426897875024E-4 60-61 0.0 0.0 0.0 0.21930454652072134 5.746426897875024E-4 62-63 0.0 0.0 0.0 0.25550703597733404 5.746426897875024E-4 64-65 2.612012226306829E-5 0.0 0.0 0.2935901742368876 6.26882934313639E-4 66-67 5.224024452613658E-5 0.0 0.0 0.3635137415351214 6.530030565767072E-4 68-69 5.224024452613658E-5 0.0 0.0 0.4609940378208922 6.791231788397755E-4 70-71 5.224024452613658E-5 0.0 0.0 0.5647431634497995 6.791231788397755E-4 72-73 5.224024452613658E-5 0.0 0.0 0.6721229860732733 7.052433011028438E-4 74-75 5.224024452613658E-5 0.0 0.0 0.7783535233171719 7.313634233659121E-4 76-77 5.224024452613658E-5 0.0 0.0 0.8728300055426899 7.313634233659121E-4 78-79 5.224024452613658E-5 0.0 0.0 0.9683251725364677 7.313634233659121E-4 80-81 5.224024452613658E-5 0.0 0.0 1.0670331145686027 7.836036678920486E-4 82-83 5.224024452613658E-5 0.0 0.0 1.2030405911923991 8.358439124181852E-4 84-85 5.224024452613658E-5 0.0 0.0 1.4173300742386115 8.880841569443218E-4 86-87 5.224024452613658E-5 0.0 0.0 1.6879084207617359 8.880841569443218E-4 88-89 5.224024452613658E-5 0.0 0.0 1.9894913524111224 8.880841569443218E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10225 0.0 29.373043 1 GTATCAA 14785 0.0 29.185001 1 GCGCGAC 90 8.887431E-4 26.420479 9 GTACATG 13165 0.0 25.91784 1 TACATGG 13470 0.0 25.082056 2 GAGTACT 8015 0.0 23.932213 12-13 ATCAACG 18005 0.0 23.759398 3 TATCAAC 18130 0.0 23.693638 2 TCAACGC 18060 0.0 23.68828 4 ACATGGG 13500 0.0 23.554743 3 CAACGCA 18325 0.0 23.32161 5 TAAGGTG 845 0.0 23.065876 5 AACGCAG 18635 0.0 22.933647 6 TAAGGTA 520 0.0 22.853115 4 AGTACTT 8295 0.0 22.493969 12-13 GTACTTT 8760 0.0 21.594738 14-15 CATGGGG 4955 0.0 21.392914 4 ACGCAGA 20605 0.0 20.694868 7 CATGGGA 8185 0.0 20.44244 4 CGCAGAG 20870 0.0 20.415718 8 >>END_MODULE