FastQCFastQC Report
Thu 26 May 2016
SRR936468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936468_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914233
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112100.5856131411379911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96530.5042750804107964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60740.3173072452517536No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33810.17662426674286777No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23180.12109288681158459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA169600.028.2402421
GGTATCA118950.028.1616691
CGCGAAA601.712939E-423.74773472-73
GTACATG127000.023.3461151
AGGTAAG7200.023.0922972
ATCAACG207950.022.8187963
TCAACGC208800.022.7229394
TATCAAC210650.022.6188772
TACATGG129450.022.6052992
ATGGGTA6950.022.5506275
GAGTACT109650.022.41519512-13
CAACGCA212400.022.3154435
GTGTAAG6400.022.2728081
AACGCAG216150.021.9485426
TAAGGTA5000.021.8467734
AGTACTT113250.021.76556612-13
ACATGGG130900.021.3002473
GTACTTT117100.021.02858414-15
ACGCAGA238800.019.8876677
CGCAGAG241200.019.689788
Produced by FastQC (version 0.11.5)