##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936468_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1914233 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.809001307573322 31.0 31.0 34.0 30.0 34.0 2 31.953742308276997 31.0 31.0 34.0 30.0 34.0 3 32.027550460158196 33.0 31.0 34.0 30.0 34.0 4 35.7577400452296 37.0 35.0 37.0 35.0 37.0 5 35.58232200573284 37.0 35.0 37.0 33.0 37.0 6 35.67950453262482 37.0 35.0 37.0 33.0 37.0 7 35.616661608069656 37.0 35.0 37.0 33.0 37.0 8 35.581828335422074 37.0 35.0 37.0 33.0 37.0 9 37.18222442095607 39.0 37.0 39.0 33.0 39.0 10-11 37.07318022414199 39.0 37.0 39.0 33.0 39.0 12-13 36.95785753353954 39.0 37.0 39.0 33.0 39.0 14-15 38.03859039103391 40.0 37.0 41.0 33.0 41.0 16-17 37.93603495499242 40.0 37.0 41.0 33.0 41.0 18-19 38.10500341389998 40.0 37.0 41.0 33.0 41.0 20-21 38.12703625943132 40.0 37.0 41.0 33.0 41.0 22-23 38.03692967366042 40.0 37.0 41.0 33.0 41.0 24-25 37.881043477988314 40.0 37.0 41.0 33.0 41.0 26-27 37.66569795839901 39.5 37.0 41.0 32.0 41.0 28-29 37.5557497441534 39.0 36.5 41.0 32.0 41.0 30-31 37.481423891448955 39.0 36.5 41.0 32.0 41.0 32-33 37.280137005265296 39.0 36.0 41.0 31.5 41.0 34-35 37.07761960012182 39.0 35.5 41.0 31.0 41.0 36-37 36.83371773446597 39.0 35.0 41.0 31.0 41.0 38-39 36.63272130404188 39.0 35.0 41.0 30.5 41.0 40-41 36.39378722443924 39.0 35.0 40.0 30.0 41.0 42-43 36.05781976384275 38.5 35.0 40.0 30.0 41.0 44-45 35.71125928766247 38.0 35.0 40.0 28.5 41.0 46-47 35.22876447120073 38.0 34.5 40.0 26.5 41.0 48-49 34.754088452137225 38.0 34.0 40.0 25.5 41.0 50-51 34.41848484484386 38.0 33.5 40.0 24.0 41.0 52-53 34.02142920950584 38.0 33.0 40.0 23.0 41.0 54-55 33.79019638675125 37.0 33.0 40.0 23.0 41.0 56-57 33.456509474029545 37.0 33.0 40.0 22.0 41.0 58-59 33.05430373418492 36.0 32.0 39.0 20.5 41.0 60-61 32.614796108937625 36.0 31.5 39.0 19.0 40.0 62-63 32.26647356930948 35.0 31.0 39.0 18.0 40.0 64-65 31.830267005113797 35.0 31.0 38.0 17.5 40.0 66-67 31.401005781427862 35.0 30.5 38.0 14.0 40.0 68-69 31.03649190041129 35.0 30.0 37.0 13.0 39.5 70-71 30.659263005078273 34.0 30.0 37.0 9.0 39.0 72-73 30.22084850694769 34.0 29.0 36.0 7.5 39.0 74-75 29.733585200965607 34.0 29.0 36.0 6.5 38.5 76-77 28.278240684388994 32.0 27.0 34.5 2.0 36.0 78-79 29.054379743740704 33.5 29.0 35.0 2.0 37.0 80-81 28.84943630164144 34.0 29.0 35.0 2.0 36.5 82-83 28.567621339721967 33.5 28.5 35.0 2.0 36.0 84-85 28.20502258606972 33.0 27.5 35.0 2.0 36.0 86-87 27.821511278929997 33.0 27.0 35.0 2.0 35.0 88-89 27.350072587819767 33.0 26.0 35.0 2.0 35.0 90-91 26.88104060477486 32.5 25.0 34.5 2.0 35.0 92-93 26.273100505528845 32.0 24.5 34.0 2.0 35.0 94-95 25.710043134769904 32.0 23.5 34.0 2.0 35.0 96-97 24.970348437206965 32.0 19.5 34.0 2.0 35.0 98-99 24.054596801956713 31.0 8.5 34.0 2.0 35.0 100-101 20.85451875503139 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.298040640744869 1101 2 0.32491035667820967 1101 3 0.25174634634679194 1101 4 0.16974082625769427 1101 5 0.2733141463587003 1101 6 0.2670382766974839 1101 7 0.21086130090115773 1101 8 0.28356029384863035 1101 9 0.28944375976714554 1101 10-11 0.3886934313786199 1101 12-13 0.40546230144342843 1101 14-15 0.5504178356078384 1101 16-17 0.466270476380366 1101 18-19 0.3684473547444398 1101 20-21 0.3292699771350769 1101 22-23 0.3905707233663023 1101 24-25 0.3760815010100629 1101 26-27 0.4509994706836622 1101 28-29 0.3455265512443759 1101 30-31 0.4308380699076224 1101 32-33 0.47382372170500275 1101 34-35 0.577638815892719 1101 36-37 0.5674971582547599 1101 38-39 0.4798199599369539 1101 40-41 0.5043086491585242 1101 42-43 0.4492002852138768 1101 44-45 0.6746289591289027 1101 46-47 0.5805430432601 1101 48-49 0.4498109112233877 1101 50-51 0.5070022405001069 1101 52-53 0.7288345109341847 1101 54-55 0.5617034227755298 1101 56-57 0.5679707027790499 1101 58-59 0.42296446225056883 1101 60-61 0.46309120695401873 1101 62-63 0.37327587352989156 1101 64-65 0.43639580092641594 1101 66-67 0.20691515110383563 1101 68-69 0.49748609499037855 1101 70-71 0.4211400531111984 1101 72-73 0.3884119239043571 1101 74-75 0.4972316398468628 1101 76-77 0.3112210923091432 1101 78-79 0.47818419955648395 1101 80-81 0.5709644165356842 1101 82-83 0.6228412256199469 1101 84-85 0.5855322417068152 1101 86-87 0.5535464015421034 1101 88-89 0.5914401065843684 1101 90-91 0.4932458679113658 1101 92-93 0.39834547156497635 1101 94-95 0.4984750784386911 1101 96-97 0.4827200059039569 1101 98-99 0.42295493628101255 1101 100-101 0.8686446727317367 1102 1 0.2512727374927728 1102 2 0.2019013643044616 1102 3 0.24904976014610725 1102 4 0.18572277073788257 1102 5 0.19209379567114127 1102 6 0.20370911464173957 1102 7 0.22500906278018107 1102 8 0.23392637975335617 1102 9 0.2567952022356863 1102 10-11 0.32250053713391225 1102 12-13 0.29111433968621014 1102 14-15 0.35126841701316636 1102 16-17 0.3413062646553229 1102 18-19 0.29666653018258415 1102 20-21 0.299818237422798 1102 22-23 0.22566853928964292 1102 24-25 0.21549240723888374 1102 26-27 0.19280328552611792 1102 28-29 0.41231243173780996 1102 30-31 0.28618682195573797 1102 32-33 0.3272974094146335 1102 34-35 0.19674916495038275 1102 36-37 0.08178592861788303 1102 38-39 0.21755168566634353 1102 40-41 0.2779121558196991 1102 42-43 0.35844376665436783 1102 44-45 0.20255400051998862 1102 46-47 0.19552929627328552 1102 48-49 0.4340768038982361 1102 50-51 0.27314355673656365 1102 52-53 0.25354115431032653 1102 54-55 0.27814300144432025 1102 56-57 0.11455658177125372 1102 58-59 0.22396838368043603 1102 60-61 0.3715884961597453 1102 62-63 0.31715811589721454 1102 64-65 0.2631070970463689 1102 66-67 0.12958540142338393 1102 68-69 0.16345442116069364 1102 70-71 0.18078264753862783 1102 72-73 0.45667839348083916 1102 74-75 0.1686711488156476 1102 76-77 0.10397936838123556 1102 78-79 0.32877110045808067 1102 80-81 0.3424045420814892 1102 82-83 0.23852059162864592 1102 84-85 0.24896214708637388 1102 86-87 0.3886515895820075 1102 88-89 0.2900244189347809 1102 90-91 0.28749384768334707 1102 92-93 0.3503212256118644 1102 94-95 0.23582238179887227 1102 96-97 0.32831987100762916 1102 98-99 0.19825496802132392 1102 100-101 0.7713268363916512 1103 1 0.17631022249877404 1103 2 0.20214076854276897 1103 3 0.2892161935727344 1103 4 0.25892998785103316 1103 5 0.2522867185020061 1103 6 0.20414743931187473 1103 7 0.10004254938554169 1103 8 0.0887944325322465 1103 9 0.19944414265951593 1103 10-11 0.18604636880124303 1103 12-13 0.24211543924639045 1103 14-15 0.2653040527588715 1103 16-17 0.2621206888856378 1103 18-19 0.2480529756044163 1103 20-21 0.23122617424807146 1103 22-23 0.07571552049716246 1103 24-25 0.12462025609933391 1103 26-27 0.1741957285489022 1103 28-29 0.07738115424880476 1103 30-31 0.13305182595413356 1103 32-33 0.17993685363695278 1103 34-35 0.10845348322306592 1103 36-37 0.0592454448114097 1103 38-39 0.1969499263700598 1103 40-41 0.11001706385648902 1103 42-43 0.13732896257599947 1103 44-45 0.17096263706536519 1103 46-47 0.310376357448817 1103 48-49 0.15193341125529258 1103 50-51 0.013291247660198735 1103 52-53 0.10633128825674021 1103 54-55 0.10804629012885414 1103 56-57 0.15493742943219502 1103 58-59 0.062468233740410994 1103 60-61 -0.07942809720290711 1103 62-63 0.1259133637980483 1103 64-65 -0.04188590575251183 1103 66-67 -0.028881711731354898 1103 68-69 0.06846591656253409 1103 70-71 0.09558747560739889 1103 72-73 0.10904969496024819 1103 74-75 -0.04545320144424991 1103 76-77 0.03132243115613065 1103 78-79 -0.09680042093336283 1103 80-81 0.010747954716432417 1103 82-83 -0.04505822684396321 1103 84-85 0.2666340783138814 1103 86-87 0.13489584188108594 1103 88-89 0.07672248970647289 1103 90-91 -0.02621616831025264 1103 92-93 0.15631307887056067 1103 94-95 -0.052209405486213 1103 96-97 0.16664529083969626 1103 98-99 0.03431254499053438 1103 100-101 0.4629271962477084 1104 1 0.25972954707663476 1104 2 0.20865577792363155 1104 3 0.2640403023907467 1104 4 0.23713355257898883 1104 5 0.18322715002170753 1104 6 0.07800766445258489 1104 7 0.26778176139304577 1104 8 0.18173533309509793 1104 9 0.22203795128991288 1104 10-11 0.2340705245235739 1104 12-13 0.22343227789554732 1104 14-15 0.27356507527294127 1104 16-17 0.38325929113704404 1104 18-19 0.26614162476014513 1104 20-21 0.19458100539252854 1104 22-23 0.1702933816603931 1104 24-25 0.10437166322878966 1104 26-27 0.19830454656015917 1104 28-29 0.2186018390518072 1104 30-31 0.16019769837439668 1104 32-33 0.20036251029736007 1104 34-35 0.16695723556828312 1104 36-37 0.26733526657500306 1104 38-39 0.17199683068525218 1104 40-41 0.06917161357505819 1104 42-43 0.1917074803996428 1104 44-45 0.19032591098656582 1104 46-47 0.2576312824019098 1104 48-49 0.32418950881626785 1104 50-51 0.29616530957389386 1104 52-53 0.3625930143355447 1104 54-55 0.18345904810259128 1104 56-57 0.04548738252788098 1104 58-59 0.18161340259594283 1104 60-61 0.31274943563010993 1104 62-63 0.21592860770049072 1104 64-65 0.1958427527840776 1104 66-67 0.0663997141598287 1104 68-69 -0.010564336720769063 1104 70-71 0.18557457692071821 1104 72-73 0.3216177062172818 1104 74-75 0.18546260530997216 1104 76-77 0.34222416661578947 1104 78-79 0.28602204623361516 1104 80-81 0.5237885269490796 1104 82-83 0.3932297656513448 1104 84-85 0.6123212265315203 1104 86-87 0.5245792377535103 1104 88-89 0.573406354622044 1104 90-91 0.6952964958548478 1104 92-93 0.8150595610469189 1104 94-95 0.8447875457333005 1104 96-97 1.1343176641980435 1104 98-99 0.8700196275421277 1104 100-101 1.4589884063790421 1105 1 -0.03208333726258061 1105 2 0.11655751596538977 1105 3 0.13211920469954208 1105 4 0.18404364942326623 1105 5 0.2546624613594801 1105 6 0.18917283365389181 1105 7 0.06345414788639658 1105 8 0.20675144312198057 1105 9 0.14759811993887695 1105 10-11 0.14373297343177427 1105 12-13 0.22793675648301814 1105 14-15 0.19327810966554893 1105 16-17 0.1986016752369082 1105 18-19 0.13173498006448625 1105 20-21 0.13104018258442096 1105 22-23 0.1789891138841213 1105 24-25 0.05705099084229914 1105 26-27 -0.05053369035849897 1105 28-29 -0.059952147949701384 1105 30-31 0.0727609877657116 1105 32-33 0.06669174861529115 1105 34-35 0.1891591540423434 1105 36-37 0.2344368656102489 1105 38-39 0.16836056747503392 1105 40-41 0.30135449920307167 1105 42-43 0.12439909766222002 1105 44-45 0.3769982008197559 1105 46-47 0.4279337136351131 1105 48-49 0.5024745734404874 1105 50-51 0.5540243560444154 1105 52-53 0.5457672419748292 1105 54-55 0.372462804040282 1105 56-57 0.3050317679533663 1105 58-59 0.2687959199947656 1105 60-61 0.5029788193985638 1105 62-63 0.4908493233539133 1105 64-65 0.4178643540286906 1105 66-67 0.34758596448202894 1105 68-69 0.3148767328009008 1105 70-71 0.26630318740788894 1105 72-73 0.4624886142704554 1105 74-75 0.4015292501897463 1105 76-77 0.260153590889459 1105 78-79 0.4002325858734892 1105 80-81 0.42865686200081754 1105 82-83 0.5792504048972127 1105 84-85 0.5327844128186641 1105 86-87 0.5270231288313134 1105 88-89 0.5930140984818202 1105 90-91 0.6591932351854091 1105 92-93 0.2936996216206218 1105 94-95 0.4279867439142464 1105 96-97 0.4161838727262328 1105 98-99 0.30520864418608795 1105 100-101 1.0917685137178523 1106 1 0.09775629087584292 1106 2 0.1643718722569787 1106 3 0.15385913024717723 1106 4 0.11556246988945418 1106 5 0.1722937507683895 1106 6 0.04304712403192923 1106 7 -0.02384301219952789 1106 8 0.012225944117908227 1106 9 0.05654567686563183 1106 10-11 0.04867807729036144 1106 12-13 0.19331373743792568 1106 14-15 0.29864812983250744 1106 16-17 0.16590920519463026 1106 18-19 0.19100277694288792 1106 20-21 0.07478086769985737 1106 22-23 -0.033312503084367506 1106 24-25 0.12408158449445494 1106 26-27 0.07006368602713309 1106 28-29 -0.10733116745394256 1106 30-31 -0.09478950267539688 1106 32-33 0.020414930546500898 1106 34-35 0.09740626155431897 1106 36-37 -0.1577394709772051 1106 38-39 0.12716816103678497 1106 40-41 0.3510355704375172 1106 42-43 0.38597267279973835 1106 44-45 0.28576391693784586 1106 46-47 0.26148956521712563 1106 48-49 0.07019503500560376 1106 50-51 0.2114927455329365 1106 52-53 0.011140772440008107 1106 54-55 0.1339811713421568 1106 56-57 0.026516173235009433 1106 58-59 0.08708749511802694 1106 60-61 -0.07523768687016741 1106 62-63 0.2137827042886684 1106 64-65 0.01370826215456944 1106 66-67 0.07146661986344682 1106 68-69 0.0872169860530434 1106 70-71 0.2601456734719356 1106 72-73 0.3619711328243156 1106 74-75 0.12544142004401593 1106 76-77 0.15788728623283887 1106 78-79 0.15996543975743904 1106 80-81 0.1723426383230482 1106 82-83 0.11887433372142908 1106 84-85 0.676331456450562 1106 86-87 0.60010944697245 1106 88-89 0.5350145866111014 1106 90-91 0.6573115681204023 1106 92-93 0.4712013244373061 1106 94-95 0.23953916754973292 1106 96-97 0.2757997780116668 1106 98-99 0.6451870073151689 1106 100-101 0.8310519252124422 1107 1 -0.017437502291908658 1107 2 0.017001070690565 1107 3 -0.027643107482962392 1107 4 -0.0363380318067712 1107 5 0.03856584118129547 1107 6 -0.11937321958874492 1107 7 -0.13368924893990197 1107 8 -0.11394491948814078 1107 9 -0.21589258831973268 1107 10-11 -0.13664201853038804 1107 12-13 -0.17853026515841464 1107 14-15 0.010580718212089835 1107 16-17 0.04648044498319592 1107 18-19 -0.09080441427544628 1107 20-21 -0.0558674733675133 1107 22-23 -0.18443896621561606 1107 24-25 -0.1868847948653496 1107 26-27 -0.2112713719243402 1107 28-29 -0.2331526098574912 1107 30-31 -0.2526826116141194 1107 32-33 -0.14345207064408072 1107 34-35 -0.10126894123997943 1107 36-37 -0.1455163407728648 1107 38-39 -0.24752814061209705 1107 40-41 -0.3273912452079486 1107 42-43 -0.36682079170139303 1107 44-45 -0.2393310924577534 1107 46-47 -0.16827285077833665 1107 48-49 -0.17277877476582404 1107 50-51 -0.09167553428374475 1107 52-53 0.18077613718284624 1107 54-55 0.06911131561119532 1107 56-57 -0.20435475294858918 1107 58-59 -0.16299408879326194 1107 60-61 0.08272288568751662 1107 62-63 -0.009257959463695897 1107 64-65 -0.1494930681114468 1107 66-67 -0.1689870539228373 1107 68-69 -0.10785552501009121 1107 70-71 -0.14433357462922203 1107 72-73 -0.024263464850456273 1107 74-75 0.12885741013201724 1107 76-77 0.06879922402107042 1107 78-79 -0.10494020979394136 1107 80-81 -0.003249360536567991 1107 82-83 0.020909874721379396 1107 84-85 0.17092125523415547 1107 86-87 0.12178790595098477 1107 88-89 -0.05563712987853009 1107 90-91 0.15682419390306634 1107 92-93 0.26752440031213 1107 94-95 0.3695543367780765 1107 96-97 0.35267300288920467 1107 98-99 0.5007996218016046 1107 100-101 0.917900576298674 1108 1 -4.7457222057545323E-4 1108 2 0.11526843110538465 1108 3 0.0845066762280382 1108 4 0.06296959273844749 1108 5 0.05654307354156174 1108 6 -0.028043937496327942 1108 7 0.027625995853405527 1108 8 -0.0058827829539183085 1108 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70-71 -1.7748306428594987 2316 72-73 -1.172947607501257 2316 74-75 -1.0746147165617046 2316 76-77 -1.1450243711367811 2316 78-79 -1.4827645099521334 2316 80-81 -1.2213384227141582 2316 82-83 -1.3079932049264649 2316 84-85 -1.229049298105135 2316 86-87 -1.4799470827002317 2316 88-89 -1.6320748577140058 2316 90-91 -1.6030662301288459 2316 92-93 -1.8694262152735313 2316 94-95 -1.9144146432067934 2316 96-97 -1.8236039504776045 2316 98-99 -2.7733681338899885 2316 100-101 -3.692727518824789 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 13.0 4 33.0 5 88.0 6 191.0 7 548.0 8 1105.0 9 2055.0 10 3185.0 11 5081.0 12 7112.0 13 9572.0 14 12286.0 15 16379.0 16 20336.0 17 26057.0 18 22308.0 19 14649.0 20 13702.0 21 13780.0 22 14771.0 23 16597.0 24 19479.0 25 23091.0 26 28195.0 27 34702.0 28 42639.0 29 52333.0 30 63777.0 31 77816.0 32 95514.0 33 120461.0 34 157193.0 35 209610.0 36 282656.0 37 331706.0 38 167623.0 39 7583.0 >>END_MODULE >>Per base sequence content fail #Base G A 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22.809748243682062 18-19 22.499075988365945 25.56428704829544 30.016704040706138 21.919932922632473 20-21 23.52192758143862 24.522720065948082 29.906103384488723 22.049248968124573 22-23 22.991289193600668 24.681027347005834 30.01345448552069 22.314228973872805 24-25 23.049623530677824 24.954433446712077 30.11007541924102 21.88586760336908 26-27 22.599430100305796 25.300223879836786 29.959202203117602 22.141143816739817 28-29 22.236850044706284 25.422188996168295 30.16304014214064 22.177920816984784 30-31 22.270719431281847 25.064890049339162 30.109165737427055 22.55522478195193 32-33 22.218808881755702 25.217123719050385 29.912042918228465 22.652024480965448 34-35 23.021515083126456 25.055610931400825 29.590109038173807 22.332764947298912 36-37 23.347343438963314 24.633459120416816 29.567104191878286 22.452093248741583 38-39 22.784371037024556 24.955144505348834 29.59326403554483 22.66722042208178 40-41 22.67630153678181 25.113524907567154 29.648435204279533 22.561738351371506 42-43 22.90706528482521 25.26975295339224 28.930640179195322 22.89254158258723 44-45 22.73794065951116 25.373232183526145 28.857106959834976 23.031720197127715 46-47 23.355621709343175 25.725586810129315 28.129612666003734 22.789178814523776 48-49 23.51353673944405 25.882808971712446 27.56731017625227 23.036344112591234 50-51 23.373563587675676 26.1154740467582 27.4650499338899 23.045912431676218 52-53 23.787599024881303 26.13454976770015 27.185887160284157 22.891964047134394 54-55 23.011302342225623 26.039896458320815 27.420887522496283 23.527913676957272 56-57 23.389880277502893 26.29537401132541 27.02928430879773 23.28546140237397 58-59 23.788865882414417 25.632429113568524 27.128108323481193 23.45059668053587 60-61 23.365160861519556 25.907374551580375 27.804642532714286 22.922822054185783 62-63 23.32832448948456 25.976463310392965 27.67619600950496 23.01901619061751 64-65 23.117489630870484 26.027769098374275 27.518295543965216 23.336445726790025 66-67 23.188505738119215 26.336628490901422 27.086665386740222 23.38820038423914 68-69 23.30397650617972 25.976207883636317 27.48111422728646 23.2387013828975 70-71 23.312402375890045 26.152290999514165 27.268431693161222 23.26687493143457 72-73 23.40002497083688 26.098729882934833 27.14784459363098 23.353400552597307 74-75 23.171033687250418 26.6904747476225 26.804073355079627 23.334418210047456 76-77 23.30392303344196 26.401825949674585 27.005295212262993 23.28895580462046 78-79 23.31539718664251 26.334918485359548 27.10147585281597 23.248208475181976 80-81 23.346381876806213 26.78638513720042 26.747746330880894 23.11948665511247 82-83 23.417546413509275 26.642789907597518 26.6556696678511 23.283994011042108 84-85 23.126875828421483 26.560228087149483 26.58894277937841 23.723953305050625 86-87 23.324885070713055 26.34776434519695 26.488229119808064 23.839121464281927 88-89 23.271847072906805 26.481224474543723 26.310220739585926 23.936707712963543 90-91 23.04510348814176 26.467913475645098 26.69221051883358 23.79477251737956 92-93 23.19790784287187 26.224653445323348 26.86785746199748 23.7095812498073 94-95 22.912370117153564 26.05323109668455 27.007349238960398 24.02704954720149 96-97 23.087440905063936 25.916784921873166 26.99940153718702 23.996372635875886 98-99 22.867643004112807 26.171713394075436 26.528032917087106 24.432610684724647 100-101 22.72363029434042 26.043656827789242 26.881974766041992 24.350738111828345 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 861.0 1 664.5 2 605.5 3 736.0 4 579.5 5 507.0 6 900.5 7 1813.5 8 2931.5 9 3510.5 10 3081.0 11 2516.0 12 2471.5 13 2539.5 14 2731.0 15 2988.0 16 3196.0 17 3390.0 18 3596.5 19 3806.0 20 4095.0 21 4522.5 22 5216.0 23 6003.0 24 6795.0 25 7954.0 26 9722.0 27 12027.5 28 14024.0 29 16063.0 30 18777.5 31 22221.0 32 27144.0 33 29861.0 34 31092.0 35 32969.5 36 35693.0 37 39311.5 38 41375.0 39 41907.5 40 43021.5 41 47630.0 42 52543.0 43 55848.5 44 58067.0 45 61122.0 46 63090.0 47 66420.0 48 77446.5 49 79299.0 50 71454.0 51 67048.0 52 66191.5 53 68344.0 54 69123.0 55 66428.5 56 61866.0 57 57180.0 58 54575.5 59 50745.0 60 43992.0 61 37985.0 62 32885.0 63 25801.5 64 19076.5 65 15264.5 66 11659.0 67 9340.0 68 7141.5 69 4844.0 70 3708.5 71 2959.5 72 2537.5 73 2353.0 74 1993.5 75 1693.0 76 1269.5 77 794.0 78 547.5 79 386.0 80 270.0 81 200.0 82 135.5 83 85.5 84 49.0 85 26.5 86 13.0 87 5.0 88 1.5 89 1.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02016473438708872 2 0.0134257428432171 3 0.009246523281126174 4 0.0 5 0.00365681711682956 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011231652573119365 14-15 3.6568171168295603E-4 16-17 5.2240244526136584E-5 18-19 0.0013321262354164828 20-21 0.0 22-23 7.836036678920487E-5 24-25 0.0 26-27 0.0019067689252039851 28-29 0.003787417728144902 30-31 0.0017500481916255754 32-33 0.009115922669810832 34-35 0.0021418500255716 36-37 0.0010186847682596633 38-39 0.0015933274580471656 40-41 2.8732134489375114E-4 42-43 0.006294949465399457 44-45 0.0022202103923608047 46-47 3.3956158941988773E-4 48-49 8.619640346812535E-4 50-51 1.0448048905227317E-4 52-53 2.350811003676146E-4 54-55 0.0014627268467318241 56-57 0.01603775506952393 58-59 0.027191047275854092 60-61 0.022384944779449523 62-63 0.014888469689948924 64-65 0.019799052675405764 66-67 0.003160534793831263 68-69 0.0014366067244687558 70-71 1.5672073357840975E-4 72-73 0.0 74-75 0.0019328890474670534 76-77 0.0026642524708329657 78-79 0.011545094040276184 80-81 0.018493046562252348 82-83 0.01972069230861656 84-85 0.03022098145837001 86-87 0.012015256241011412 88-89 0.04307208161179961 90-91 0.048165505453097925 92-93 0.03186654916094331 94-95 0.03309419490730752 96-97 0.0521357640370843 98-99 0.055505259809020116 100-101 0.06143452756273662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1914233.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.861616423799056 #Duplication Level Percentage of deduplicated Percentage of total 1 69.47699889423532 29.084194779871897 2 15.036128345916111 12.58873274631505 3 5.938816110787673 7.45825326123815 4 3.032564375609321 5.077921866889403 5 1.7130154905207504 3.5854798696102823 6 1.0472922227765131 2.6304807188098978 7 0.7170209175475086 2.1010958232749974 8 0.48745864672936035 1.6324645513478921 9 0.3635066646048975 1.3695278905066313 >10 1.8828281335855253 14.408284354042117 >50 0.17331825405412044 5.046311263903065 >100 0.11902498914294327 9.225013679576145 >500 0.008519092763795642 2.4625342150674094 >1k 0.0031320193984542798 1.9170059240412953 >5k 2.505615518763424E-4 0.8247955617157949 >10k+ 1.252807759381712E-4 0.5879034937899192 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11210 0.5856131411379911 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9653 0.5042750804107964 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6074 0.3173072452517536 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3381 0.17662426674286777 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2318 0.12109288681158459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0448048905227315E-4 2 0.0 0.0 0.0 0.0 1.0448048905227315E-4 3 0.0 0.0 0.0 0.0 1.0448048905227315E-4 4 0.0 0.0 0.0 0.0 1.0448048905227315E-4 5 0.0 0.0 0.0 0.0 1.0448048905227315E-4 6 0.0 0.0 0.0 0.0 1.5672073357840975E-4 7 0.0 0.0 0.0 0.0 1.5672073357840975E-4 8 0.0 0.0 0.0 0.0 1.5672073357840975E-4 9 0.0 0.0 0.0 0.0 1.5672073357840975E-4 10-11 0.0 0.0 0.0 0.0 1.5672073357840975E-4 12-13 0.0 0.0 0.0 2.612012226306829E-5 2.350811003676146E-4 14-15 0.0 0.0 0.0 5.224024452613658E-5 2.612012226306829E-4 16-17 0.0 0.0 0.0 1.0448048905227315E-4 3.134414671568195E-4 18-19 0.0 0.0 0.0 1.0448048905227315E-4 3.6568171168295603E-4 20-21 0.0 0.0 0.0 1.8284085584147801E-4 4.701622007352292E-4 22-23 0.0 0.0 0.0 2.612012226306829E-4 4.701622007352292E-4 24-25 0.0 0.0 0.0 3.6568171168295603E-4 4.701622007352292E-4 26-27 0.0 0.0 0.0 5.485225675244341E-4 5.224024452613658E-4 28-29 0.0 0.0 0.0 0.0011492853795750048 5.224024452613658E-4 30-31 0.0 0.0 0.0 0.002585892104043761 5.224024452613658E-4 32-33 0.0 0.0 0.0 0.004257579928880132 5.224024452613658E-4 34-35 0.0 0.0 0.0 0.006373309832188662 5.224024452613658E-4 36-37 0.0 0.0 0.0 0.009951766582229018 5.224024452613658E-4 38-39 0.0 0.0 0.0 0.015254151401631881 5.224024452613658E-4 40-41 0.0 0.0 0.0 0.020843857565928495 5.224024452613658E-4 42-43 0.0 0.0 0.0 0.026747005197381928 5.224024452613658E-4 44-45 0.0 0.0 0.0 0.03168370830510184 5.224024452613658E-4 46-47 0.0 0.0 0.0 0.0402772285296513 5.224024452613658E-4 48-49 0.0 0.0 0.0 0.046310976772420076 6.26882934313639E-4 50-51 0.0 0.0 0.0 0.053232809172133175 6.791231788397755E-4 52-53 0.0 0.0 0.0 0.06825187947339745 7.313634233659121E-4 54-55 0.0 0.0 0.0 0.09322271635689072 7.313634233659121E-4 56-57 0.0 0.0 0.0 0.1278318783554562 7.836036678920486E-4 58-59 0.0 0.0 0.0 0.16980691483220695 7.836036678920486E-4 60-61 0.0 0.0 0.0 0.21930454652072134 7.836036678920486E-4 62-63 0.0 0.0 0.0 0.2554547957328078 7.836036678920486E-4 64-65 5.224024452613658E-5 0.0 0.0 0.2936946547259398 8.358439124181852E-4 66-67 5.224024452613658E-5 0.0 0.0 0.36395778361359354 8.358439124181852E-4 68-69 5.224024452613658E-5 0.0 0.0 0.4614119597771013 8.358439124181852E-4 70-71 5.224024452613658E-5 0.0 0.0 0.5650043646724301 8.358439124181852E-4 72-73 5.224024452613658E-5 0.0 0.0 0.6723058269291147 8.358439124181852E-4 74-75 5.224024452613658E-5 2.612012226306829E-5 0.0 0.7777788806273844 8.358439124181852E-4 76-77 5.224024452613658E-5 5.224024452613658E-5 0.0 0.8718374408966933 8.358439124181852E-4 78-79 5.224024452613658E-5 5.224024452613658E-5 0.0 0.9665490042225791 8.358439124181852E-4 80-81 5.224024452613658E-5 5.224024452613658E-5 0.0 1.0646823035649264 8.358439124181852E-4 82-83 5.224024452613658E-5 5.224024452613658E-5 0.0 1.2000628972544094 9.403244014704584E-4 84-85 5.224024452613658E-5 5.224024452613658E-5 0.0 1.4129157735761528 9.403244014704584E-4 86-87 7.836036678920486E-5 5.224024452613658E-5 0.0 1.6802291048163938 0.0010186847682596633 88-89 1.0448048905227315E-4 5.224024452613658E-5 0.0 1.9792000242394736 0.0011231652573119365 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16960 0.0 28.240242 1 GGTATCA 11895 0.0 28.161669 1 CGCGAAA 60 1.712939E-4 23.747734 72-73 GTACATG 12700 0.0 23.346115 1 AGGTAAG 720 0.0 23.092297 2 ATCAACG 20795 0.0 22.818796 3 TCAACGC 20880 0.0 22.722939 4 TATCAAC 21065 0.0 22.618877 2 TACATGG 12945 0.0 22.605299 2 ATGGGTA 695 0.0 22.550627 5 GAGTACT 10965 0.0 22.415195 12-13 CAACGCA 21240 0.0 22.315443 5 GTGTAAG 640 0.0 22.272808 1 AACGCAG 21615 0.0 21.948542 6 TAAGGTA 500 0.0 21.846773 4 AGTACTT 11325 0.0 21.765566 12-13 ACATGGG 13090 0.0 21.300247 3 GTACTTT 11710 0.0 21.028584 14-15 ACGCAGA 23880 0.0 19.887667 7 CGCAGAG 24120 0.0 19.68978 8 >>END_MODULE