FastQCFastQC Report
Thu 26 May 2016
SRR936467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033403
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62710.3083992695987957No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59250.2913834591568912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49960.24569649990680648No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35970.17689557849575316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29550.145322889756728No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26430.12997915317327652No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG23890.11748777787777434No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23540.11576652537642564No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG21870.10755369201284742No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG21720.10681601236941225No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG21300.1047505093677938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG99050.029.1107391
TACATGG101650.027.480272
GTGTAAG5300.026.8883651
ACATGGG103150.026.20563
ATGGGAG23450.022.4869825
GATGTGC13350.022.4192498
ATTCGCG5850.021.92975480-81
GTATCAA147100.021.5716231
CATGGGA53350.021.1930564
CATGGGG52750.021.1639374
GTGTAGG10950.020.8231351
GAGTACT59250.020.72637212-13
CGCGGAA6100.020.636282-83
AGTACTT62900.020.61878412-13
AGAGTAC115200.020.2898910-11
GTACTTT62100.020.00564414-15
AATTCGC6250.019.76796578-79
GTAAGGT5650.019.3388423
ATGGGGA29300.018.8079515
TACTTTT68150.018.75248514-15
Produced by FastQC (version 0.11.5)