##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936467_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2033403 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.393143907036627 31.0 31.0 34.0 30.0 34.0 2 31.46928621625915 31.0 31.0 34.0 28.0 34.0 3 31.4823490473851 31.0 31.0 34.0 28.0 34.0 4 35.11552358288052 37.0 35.0 37.0 32.0 37.0 5 35.1209986411941 37.0 35.0 37.0 32.0 37.0 6 35.112667779087566 37.0 35.0 37.0 32.0 37.0 7 35.00845331692734 37.0 35.0 37.0 32.0 37.0 8 34.961152806403845 37.0 35.0 37.0 32.0 37.0 9 36.45961917042514 39.0 37.0 39.0 32.0 39.0 10-11 36.312050046154155 39.0 36.0 39.0 32.0 39.0 12-13 36.208319010053586 39.0 35.0 39.0 32.0 39.0 14-15 37.299752434711664 39.5 37.0 41.0 32.0 41.0 16-17 37.18797823156551 39.0 36.0 41.0 31.0 41.0 18-19 37.12322594193084 39.0 36.0 41.0 31.0 41.0 20-21 36.97079993488748 39.0 36.0 41.0 31.0 41.0 22-23 37.087665357039405 39.0 36.0 41.0 31.0 41.0 24-25 37.1189454328532 39.0 36.0 41.0 31.0 41.0 26-27 37.01923180009078 39.0 36.0 41.0 31.0 41.0 28-29 36.865252731504775 39.0 36.0 41.0 30.0 41.0 30-31 36.65613555207699 39.0 36.0 41.0 30.0 41.0 32-33 36.48279042570509 39.0 35.0 41.0 30.0 41.0 34-35 36.28594676018477 39.0 35.0 40.0 30.0 41.0 36-37 36.06800447328936 38.5 35.0 40.0 29.0 41.0 38-39 35.85567691205334 38.0 35.0 40.0 28.5 41.0 40-41 35.62138469354083 38.0 35.0 40.0 27.5 41.0 42-43 35.35898884775915 38.0 34.0 40.0 27.0 41.0 44-45 35.07571838932076 38.0 34.0 40.0 26.0 41.0 46-47 34.873248195266754 38.0 34.0 40.0 25.5 41.0 48-49 34.69117976121802 38.0 34.0 40.0 24.0 41.0 50-51 33.684791209612655 37.0 32.0 39.5 23.5 40.5 52-53 33.77037606416436 37.5 33.0 39.5 23.0 40.0 54-55 34.16424633975656 38.0 33.0 40.0 23.5 41.0 56-57 34.02089108750208 37.5 33.0 40.0 23.0 41.0 58-59 33.7590165353351 37.0 33.0 40.0 23.0 41.0 60-61 33.42058804870456 36.5 33.0 40.0 22.0 41.0 62-63 33.0219341665179 36.0 32.0 39.0 21.0 41.0 64-65 32.64088230419647 35.5 32.0 39.0 20.0 41.0 66-67 32.157970653136644 35.0 31.0 38.5 18.5 40.0 68-69 31.760761147691824 35.0 31.0 38.0 18.0 40.0 70-71 31.348084959056322 35.0 31.0 37.0 16.0 39.5 72-73 30.951761406863273 35.0 30.0 36.5 12.5 39.0 74-75 30.530941234964246 34.5 30.0 36.0 9.5 39.0 76-77 30.07322773695131 34.0 29.5 35.5 7.0 37.5 78-79 29.587223732826203 34.0 29.0 35.0 4.5 37.0 80-81 29.14627449649676 34.0 29.0 35.0 2.0 37.0 82-83 28.767429033988837 34.0 28.5 35.0 2.0 36.0 84-85 28.410888053179818 34.0 27.5 35.0 2.0 36.0 86-87 28.045469835541702 33.5 27.0 35.0 2.0 35.5 88-89 27.641008693308706 33.0 26.5 35.0 2.0 35.0 90-91 27.220410317089133 33.0 25.5 35.0 2.0 35.0 92-93 26.757630927071517 33.0 25.0 35.0 2.0 35.0 94-95 26.232314007602035 33.0 24.5 35.0 2.0 35.0 96-97 25.58501339872126 32.0 21.5 35.0 2.0 35.0 98-99 24.748745329873124 32.0 10.5 34.5 2.0 35.0 100-101 21.747181940815473 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4601536166547717 1101 2 0.48133657996731216 1101 3 0.4051098946556557 1101 4 0.45394090772492035 1101 5 0.39447535581660986 1101 6 0.43882970770587804 1101 7 0.6354018362854674 1101 8 0.5447974187153619 1101 9 0.5884916593100158 1101 10-11 0.699618308546512 1101 12-13 0.7507416850054227 1101 14-15 0.7549986033301579 1101 16-17 0.8535333870992261 1101 18-19 0.8047076273112168 1101 20-21 0.8015901756637547 1101 22-23 0.7178169059646109 1101 24-25 0.7330741830644385 1101 26-27 0.5998505874935844 1101 28-29 0.693674979041198 1101 30-31 0.6710995302734588 1101 32-33 0.655418865455232 1101 34-35 0.6185720675370803 1101 36-37 0.5149758940370432 1101 38-39 0.5043145712866988 1101 40-41 0.3834534535322476 1101 42-43 0.63272874387021 1101 44-45 0.5356992015550972 1101 46-47 0.659695072790349 1101 48-49 0.6809463122955535 1101 50-51 0.43330594768935526 1101 52-53 0.7269221471718055 1101 54-55 0.683271417748621 1101 56-57 0.601097605605716 1101 58-59 0.6498203926096195 1101 60-61 0.7564784133216875 1101 62-63 0.6616892076279797 1101 64-65 0.565581705384524 1101 66-67 0.7118048278530509 1101 68-69 0.5393513235235794 1101 70-71 0.5222709686543006 1101 72-73 0.505957998844984 1101 74-75 0.40312613705566847 1101 76-77 0.3434222192106553 1101 78-79 0.33550228621426825 1101 80-81 0.35933959824022565 1101 82-83 0.2834696900378155 1101 84-85 0.2326691430633403 1101 86-87 0.23455898619502236 1101 88-89 0.26734565487605266 1101 90-91 0.3839044598623822 1101 92-93 0.3177181806184066 1101 94-95 0.3099552792684044 1101 96-97 0.28267894106059543 1101 98-99 0.31578324824226556 1101 100-101 0.4673929507504013 1102 1 0.2648868303041958 1102 2 0.2913674722224968 1102 3 0.3291684073242074 1102 4 0.29013690196628517 1102 5 0.297258668941744 1102 6 0.30414035808879447 1102 7 0.28399591157074155 1102 8 0.2642436057072217 1102 9 0.2903129658553496 1102 10-11 0.387854869291715 1102 12-13 0.36921761758267735 1102 14-15 0.3723697073319414 1102 16-17 0.25818831655515595 1102 18-19 0.22183846044688238 1102 20-21 0.06604164648749133 1102 22-23 0.22998818158557555 1102 24-25 0.3144362638145566 1102 26-27 -0.018595343835464462 1102 28-29 -0.09754181941021045 1102 30-31 0.21049116917642863 1102 32-33 0.02914250800410656 1102 34-35 0.1015116921823278 1102 36-37 -0.04544330868831281 1102 38-39 -0.1594004341135289 1102 40-41 -0.11649938731503795 1102 42-43 -0.09670826013089595 1102 44-45 -0.06463530734554013 1102 46-47 -0.058961549578825156 1102 48-49 0.011167843920475207 1102 50-51 0.006836097238050343 1102 52-53 0.1612903529928147 1102 54-55 -0.030606176355966852 1102 56-57 -0.01079340417409469 1102 58-59 -0.017751729789054593 1102 60-61 -0.013801928776821626 1102 62-63 0.06315768344014572 1102 64-65 0.2084721319962597 1102 66-67 0.17934929692729185 1102 68-69 -0.04208611153224595 1102 70-71 -0.023847672535989517 1102 72-73 -0.3475800212652871 1102 74-75 -0.2660030400913662 1102 76-77 -0.15024059405154233 1102 78-79 -0.2254014517205185 1102 80-81 -0.054438319476485475 1102 82-83 -0.026116802457057275 1102 84-85 -0.28161596632382313 1102 86-87 -0.33375516038686115 1102 88-89 -0.21423187296490198 1102 90-91 -0.1329909587965048 1102 92-93 -0.43603950080485987 1102 94-95 -0.47764130167295704 1102 96-97 -0.4321663187221212 1102 98-99 -0.48013461536383417 1102 100-101 -0.38258334926690196 1103 1 0.30695424632003565 1103 2 0.23363356065872054 1103 3 0.31894833116644605 1103 4 0.477363623168614 1103 5 0.5594332108418101 1103 6 0.3977190780650943 1103 7 0.4868625274973297 1103 8 0.5118379185305244 1103 9 0.42006721791760526 1103 10-11 0.5116019010169666 1103 12-13 0.15416077718087706 1103 14-15 0.49131648146594387 1103 16-17 0.6214079730551489 1103 18-19 0.7387782222861361 1103 20-21 0.6954222959366803 1103 22-23 0.39648809682026354 1103 24-25 0.5661272027402475 1103 26-27 0.4954986261548342 1103 28-29 0.6424173622214013 1103 30-31 0.4356403422282753 1103 32-33 0.3667527177972616 1103 34-35 0.5709524078794814 1103 36-37 0.5582941467404154 1103 38-39 0.562102639472684 1103 40-41 0.5126682570311587 1103 42-43 0.6325469684198097 1103 44-45 0.6016053491151467 1103 46-47 0.7338508880134569 1103 48-49 0.7901327661686537 1103 50-51 0.7118697869827173 1103 52-53 0.7979302481140991 1103 54-55 0.8302702254975145 1103 56-57 0.6761861675844045 1103 58-59 0.5445026344333925 1103 60-61 0.696413561716561 1103 62-63 0.7929402633944775 1103 64-65 0.6709325136426401 1103 66-67 0.8031564782762146 1103 68-69 0.5879496328282734 1103 70-71 0.4320974924466867 1103 72-73 0.2131195843675755 1103 74-75 0.3853647782321552 1103 76-77 0.4842076115640914 1103 78-79 0.36776773467939705 1103 80-81 0.5611085120676442 1103 82-83 0.23902651047304602 1103 84-85 0.2093614908677104 1103 86-87 0.4073656236307279 1103 88-89 0.14723431282239474 1103 90-91 0.24250458080120296 1103 92-93 0.22249049003572452 1103 94-95 0.1238245625222909 1103 96-97 0.10795050256679417 1103 98-99 -0.038733351467385546 1103 100-101 0.24725675993146723 1104 1 0.3724936042520248 1104 2 0.28054455945393286 1104 3 0.261505464440976 1104 4 0.41806443179395103 1104 5 0.3557035257822534 1104 6 0.4498464596931768 1104 7 0.3226775125787569 1104 8 0.429386882010931 1104 9 0.5292259052139272 1104 10-11 0.5925876982701155 1104 12-13 0.5175282393684455 1104 14-15 0.3192581601927742 1104 16-17 0.28557701694762017 1104 18-19 0.2313650043373201 1104 20-21 0.11233610399442284 1104 22-23 0.23836961473934792 1104 24-25 0.38099639184621026 1104 26-27 0.1096937151052586 1104 28-29 0.13884353799605975 1104 30-31 0.27830587372355353 1104 32-33 0.16872346051549414 1104 34-35 0.19438509737570087 1104 36-37 0.12612350410727657 1104 38-39 0.15774267220099603 1104 40-41 0.08686698246136615 1104 42-43 0.17221592590493628 1104 44-45 0.022981625671064876 1104 46-47 -0.13429928222129917 1104 48-49 0.14591910987660128 1104 50-51 0.18557226396601578 1104 52-53 0.2025277332098554 1104 54-55 0.10552615095296147 1104 56-57 -0.008465922040073792 1104 58-59 0.20587285837994074 1104 60-61 0.2081958790878744 1104 62-63 0.42450220517640247 1104 64-65 0.42101912574572964 1104 66-67 0.219804102565611 1104 68-69 0.36475700013976464 1104 70-71 0.24032416799160572 1104 72-73 0.07422878441573744 1104 74-75 0.09668991474347521 1104 76-77 -0.07862097182227501 1104 78-79 -0.02750635679445068 1104 80-81 0.043837075879263665 1104 82-83 -0.09568094537525695 1104 84-85 -0.20239879467785116 1104 86-87 -0.021087865055644528 1104 88-89 -0.0015136815852869745 1104 90-91 -0.12053854670245912 1104 92-93 -0.153342767537616 1104 94-95 -0.08037790586121574 1104 96-97 0.12937379127134818 1104 98-99 0.10986619430025257 1104 100-101 0.19027705296294073 1105 1 0.20322217364321915 1105 2 0.13912460100449664 1105 3 0.3345992634653463 1105 4 0.16463350773173602 1105 5 0.06001479305781032 1105 6 0.23816251087159657 1105 7 0.2798509065393233 1105 8 0.41514442920985317 1105 9 0.3848969068060555 1105 10-11 0.29104085296882687 1105 12-13 0.12592896796284947 1105 14-15 0.1939148592831188 1105 16-17 0.18395765415080234 1105 18-19 0.1856191416710331 1105 20-21 0.21446901075752578 1105 22-23 -0.11261068502187754 1105 24-25 0.05195920233910556 1105 26-27 0.010276774117571108 1105 28-29 -0.13093634909515828 1105 30-31 -0.1955878278880121 1105 32-33 -0.2840227799590238 1105 34-35 -0.14896335106681846 1105 36-37 -0.10546522536451874 1105 38-39 -0.15568428195576445 1105 40-41 -0.23156092664114425 1105 42-43 -0.37057877910857684 1105 44-45 -0.1426195189293935 1105 46-47 -0.005468344314458307 1105 48-49 -0.08950170427093695 1105 50-51 -0.051394603169754305 1105 52-53 0.042318904507105515 1105 54-55 0.05923380077727103 1105 56-57 -0.06439405007120058 1105 58-59 0.1566631281083275 1105 60-61 0.05022783682672838 1105 62-63 0.16796708193836452 1105 64-65 0.19740210819557547 1105 66-67 0.3266725393986576 1105 68-69 0.26916839076595167 1105 70-71 0.2440538774795975 1105 72-73 -0.004759198280147814 1105 74-75 0.12081042639423245 1105 76-77 0.21536845788746461 1105 78-79 0.353168377009208 1105 80-81 0.3909383776913309 1105 82-83 0.21749851520133134 1105 84-85 0.22851630188842975 1105 86-87 -0.1216982797189381 1105 88-89 0.011739630908820686 1105 90-91 0.002616302128377157 1105 92-93 -0.30054139875424823 1105 94-95 -0.04104289838428343 1105 96-97 0.13025305026681622 1105 98-99 0.3159826843367064 1105 100-101 0.3482325850178398 1106 1 0.3517484493347638 1106 2 0.31760025037171147 1106 3 0.3601862193216405 1106 4 0.30189342807648245 1106 5 0.11036349738442652 1106 6 0.3830988580896104 1106 7 0.4544156425920036 1106 8 0.54743822514682 1106 9 0.5465856867065426 1106 10-11 0.5532278608629255 1106 12-13 0.5592038721350576 1106 14-15 0.6030047248351096 1106 16-17 0.5941924364933229 1106 18-19 0.48955394816181297 1106 20-21 0.1320286246574085 1106 22-23 0.3867662642701717 1106 24-25 0.3596463428786052 1106 26-27 0.3518133604374114 1106 28-29 0.5035025183852966 1106 30-31 0.5690163446598362 1106 32-33 0.5547284657172398 1106 34-35 0.48367948931316107 1106 36-37 0.3189891854348659 1106 38-39 0.28492927100205634 1106 40-41 0.28386377669233553 1106 42-43 0.41814602251854893 1106 44-45 0.2890613022476032 1106 46-47 0.42612277116526087 1106 48-49 0.2636900329415326 1106 50-51 -0.008166235422308432 1106 52-53 -0.00530759328043473 1106 54-55 0.13084020448862077 1106 56-57 0.08221290632928913 1106 58-59 0.0039651123473376515 1106 60-61 0.0813742076148074 1106 62-63 0.22068002030587053 1106 64-65 0.26507549102718286 1106 66-67 0.15833831042549917 1106 68-69 0.275236704843028 1106 70-71 -0.05832638454606709 1106 72-73 -0.26958189560717116 1106 74-75 0.15004449279175347 1106 76-77 0.18913910720365834 1106 78-79 -0.0022677032174449607 1106 80-81 0.24138962710887668 1106 82-83 0.0887191844143338 1106 84-85 0.11998601282351018 1106 86-87 0.14961128741493113 1106 88-89 0.002018679399213852 1106 90-91 -0.0699987592456246 1106 92-93 0.17811629790955052 1106 94-95 0.23359895905933215 1106 96-97 0.17512928191328214 1106 98-99 0.252522280686577 1106 100-101 0.08050784461167382 1107 1 0.21483194362010138 1107 2 0.2799262318328104 1107 3 0.2914181771210629 1107 4 0.35800856587216145 1107 5 0.3407316794228734 1107 6 0.42400316606521926 1107 7 0.43845543408127696 1107 8 0.49245927005416235 1107 9 0.5897969734226365 1107 10-11 0.6164270502186326 1107 12-13 0.44532989582442895 1107 14-15 0.3233886905540686 1107 16-17 0.33716939093525156 1107 18-19 0.36259050676564897 1107 20-21 0.4798917704556942 1107 22-23 0.3982756306384658 1107 24-25 0.4375220187039517 1107 26-27 0.45577321243079183 1107 28-29 0.45338111211474796 1107 30-31 0.5225531351730126 1107 32-33 0.25954335360464853 1107 34-35 0.185205245095311 1107 36-37 0.22187325499381672 1107 38-39 0.15338042812582842 1107 40-41 0.257325733176458 1107 42-43 0.24703759970974204 1107 44-45 0.47915081233047374 1107 46-47 0.3546860408792796 1107 48-49 0.3235858121915385 1107 50-51 0.3060657292997746 1107 52-53 0.46480898442995766 1107 54-55 0.5814937042428667 1107 56-57 0.44963994142027985 1107 58-59 0.5765471113366232 1107 60-61 0.6499652891430614 1107 62-63 0.8839909241984927 1107 64-65 0.7096578119204793 1107 66-67 0.6208901438938312 1107 68-69 0.8168631335084093 1107 70-71 0.5756968790760091 1107 72-73 0.4918431007584303 1107 74-75 0.5951455810349877 1107 76-77 0.5892329093399944 1107 78-79 0.6591282179472238 1107 80-81 0.743913804522986 1107 82-83 0.5886226997445796 1107 84-85 0.5962568413975085 1107 86-87 0.4623280534218672 1107 88-89 0.15937435910126396 1107 90-91 0.3792844851314392 1107 92-93 0.5599541621283635 1107 94-95 0.5011730142883692 1107 96-97 0.5302941106118908 1107 98-99 0.7515241186546362 1107 100-101 0.7689186079741503 1108 1 0.2664039764807491 1108 2 0.17721424171284994 1108 3 0.21033727459837337 1108 4 0.2614892189273448 1108 5 0.18268758568933663 1108 6 0.20887620366680437 1108 7 0.28096057406320085 1108 8 0.20027618275287296 1108 9 0.2912732537494094 1108 10-11 0.34054699898617713 1108 12-13 0.15382905633809685 1108 14-15 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-2.470021342299546 2316 82-83 -2.444197415551276 2316 84-85 -2.0309896012708677 2316 86-87 -1.884953940287648 2316 88-89 -2.1391960999734607 2316 90-91 -2.2954279434769056 2316 92-93 -3.017341508485451 2316 94-95 -2.8586394591671507 2316 96-97 -3.412817541041367 2316 98-99 -4.730869280197158 2316 100-101 -5.259083776945317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4366.0 3 1878.0 4 2060.0 5 2253.0 6 2564.0 7 3139.0 8 3807.0 9 4671.0 10 5818.0 11 7135.0 12 8691.0 13 10109.0 14 11534.0 15 13465.0 16 16244.0 17 20163.0 18 17045.0 19 14194.0 20 14972.0 21 16056.0 22 17697.0 23 20052.0 24 22953.0 25 26707.0 26 30946.0 27 36407.0 28 42626.0 29 52331.0 30 63130.0 31 76896.0 32 95004.0 33 122009.0 34 160550.0 35 217399.0 36 292427.0 37 343172.0 38 211381.0 39 21550.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.191131047236816 17.476784906254302 13.836933640244741 23.49515040626414 2 16.298556940359436 19.380428688065003 38.55772730402621 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46-47 24.166046190934726 25.541633511787726 26.3688989695021 23.923421327775444 48-49 24.551254529994477 25.51068633627276 26.045408876108922 23.892650257623842 50-51 23.899596401672426 25.77803887065255 26.173301133335976 24.149063594339047 52-53 24.494572941922858 25.77719799540655 25.745230729886444 23.982998332784152 54-55 23.888894353486222 25.41162352640302 26.084055889715685 24.615426230395073 56-57 24.126712236135024 25.849042406752503 25.916663838861325 24.10758151825115 58-59 24.63884832785534 25.20640224424786 25.86115486893882 24.29359455895798 60-61 24.26137122294268 25.317340314275167 26.728094167961125 23.693194294821023 62-63 23.875509187380466 25.684904054171685 26.43813007558325 24.0014566828646 64-65 23.749023183440862 25.776793543396227 26.080104613426535 24.39407865973637 66-67 23.849606175471592 25.939223604829238 25.765029385283526 24.44614083441564 68-69 23.637104306817548 25.59249890470449 26.38566258797726 24.3847342005007 70-71 23.976692354380727 25.71686829836916 26.073509523349102 24.232929823901014 72-73 24.061229147442997 25.54567346946806 26.24038160744957 24.152715775639376 74-75 23.683170313850937 26.34985173897697 25.707784964327573 24.25919298284452 76-77 24.006359613307175 25.976865725973862 25.759336254715105 24.257438406003853 78-79 24.085123196922435 25.895747801271934 25.90913195173144 24.109997050074192 80-81 24.013932761677324 26.4097889610869 25.736480427294982 23.83979784994079 82-83 24.05914024787163 26.116044740686363 25.718243692380966 24.106571319061043 84-85 23.853512450403862 26.08715540938193 25.569484023115063 24.489848117099143 86-87 24.078781974773104 25.893119550463755 25.744326751936825 24.283771722826316 88-89 23.821877244242444 26.049838168599788 25.37238438154827 24.7559002056095 90-91 23.929796837285824 25.934684274692284 25.781256918621757 24.354261969400135 92-93 24.17912863267508 25.77548022257033 25.84685659866234 24.198534546092244 94-95 23.87071848367713 25.655578175314997 25.86705024619687 24.606653094811005 96-97 23.802124642931364 25.713393317175324 25.970593407187977 24.513888632705335 98-99 23.767026518920844 25.981885449342162 25.408022664222003 24.843065367514996 100-101 23.66618774712359 25.579101817933964 25.74703943815085 25.007670996791592 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 283.0 1 217.5 2 229.0 3 311.0 4 298.0 5 309.0 6 482.0 7 910.0 8 1490.5 9 1799.5 10 1680.5 11 1540.5 12 1617.0 13 1784.5 14 1929.0 15 2045.0 16 2128.5 17 2239.5 18 2378.5 19 2506.0 20 2699.5 21 2982.5 22 3367.5 23 3880.0 24 4526.0 25 5419.0 26 6517.5 27 7868.5 28 9298.0 29 11202.5 30 13440.0 31 16260.0 32 20315.5 33 22693.5 34 24612.0 35 26742.5 36 29653.5 37 33834.5 38 36599.0 39 37998.0 40 40137.0 41 46677.0 42 53450.0 43 58019.5 44 61506.5 45 65821.0 46 68874.0 47 74573.5 48 90061.0 49 93251.5 50 84120.0 51 79611.5 52 78991.5 53 82383.5 54 84395.0 55 80670.0 56 74815.0 57 69135.5 58 64912.5 59 59690.5 60 51902.5 61 44082.5 62 37194.0 63 30112.0 64 23821.0 65 19749.5 66 15492.0 67 12700.5 68 9806.0 69 6557.0 70 5105.5 71 4196.0 72 3577.0 73 3196.5 74 2563.5 75 2108.5 76 1490.5 77 893.5 78 606.5 79 424.0 80 274.0 81 162.5 82 102.0 83 72.0 84 45.0 85 24.0 86 19.5 87 14.5 88 14.0 89 12.0 90 9.0 91 8.5 92 5.5 93 3.0 94 2.0 95 3.5 96 3.5 97 2.0 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011950410223649715 2 0.010966837365736157 3 0.014360163725537929 4 0.017212525013487242 5 0.028917042022658566 6 0.04568695925008471 7 0.005409650718524562 8 0.02409753501888214 9 0.033293941240373895 10-11 0.037597072493745706 12-13 0.028425255593701793 14-15 0.027171200199862005 16-17 0.027367914771444714 18-19 0.02198285337436799 20-21 0.05239984400534474 22-23 0.012319250045367299 24-25 0.0026310573949187644 26-27 0.0011802874294962681 28-29 1.7212525013487242E-4 30-31 0.0026556467163666034 32-33 0.0018687884300357578 34-35 0.0 36-37 2.2130389303055026E-4 38-39 4.917864289567784E-5 40-41 0.0014261806439746572 42-43 0.0042785419319239714 44-45 0.001844199108587919 46-47 8.360369292265233E-4 48-49 5.901437147481341E-4 50-51 0.0034179156812496098 52-53 0.003590040931384482 54-55 0.0055571866472115955 56-57 0.0011802874294962681 58-59 0.005704722575898629 60-61 0.002827771966501476 62-63 7.868582863308454E-4 64-65 5.901437147481341E-4 66-67 0.016106005548334492 68-69 0.04162972121119129 70-71 0.054514525649858876 72-73 0.015687987083721232 74-75 0.03297428006155199 76-77 0.028277719665014755 78-79 0.05648167136568599 80-81 0.08089886756339004 82-83 0.04819507003776428 84-85 0.017532186192309146 86-87 0.01841740176443135 88-89 0.020802565944871725 90-91 0.04155595324684777 92-93 0.02544994769851328 94-95 0.013474948153415729 96-97 0.01748300754941347 98-99 0.046645942786550425 100-101 0.06877633208960544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2033403.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.95230665008251 #Duplication Level Percentage of deduplicated Percentage of total 1 74.77497126479716 33.613074380462706 2 13.300801958695253 11.958034566785742 3 4.672722536832788 6.301489690993771 4 2.2045897378835067 3.964055757398577 5 1.2536371176272985 2.817694006975395 6 0.7430429976302342 2.0040898010202497 7 0.5089225370144406 1.601406936150777 8 0.3580802011840536 1.2877224807159053 9 0.2776518125975232 1.1232980479651595 >10 1.597332347526779 13.532624315428176 >50 0.16952394613791555 5.328143091734697 >100 0.12780755929145363 11.086097140483888 >500 0.008049159020814151 2.4598374129482776 >1k 0.002646298856158077 2.3179313415113683 >5k 2.2052490467983974E-4 0.6045010294252677 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6271 0.3083992695987957 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5925 0.2913834591568912 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4996 0.24569649990680648 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3597 0.17689557849575316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2955 0.145322889756728 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2643 0.12997915317327652 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2389 0.11748777787777434 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2354 0.11576652537642564 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2187 0.10755369201284742 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2172 0.10681601236941225 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2130 0.1047505093677938 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.458932144783892E-5 0.0 18-19 0.0 0.0 0.0 4.917864289567784E-5 0.0 20-21 0.0 0.0 0.0 4.917864289567784E-5 0.0 22-23 0.0 0.0 0.0 1.9671457158271135E-4 0.0 24-25 0.0 0.0 0.0 2.704825359262281E-4 0.0 26-27 0.0 0.0 0.0 5.163757504046173E-4 0.0 28-29 0.0 0.0 0.0 0.0010819301437049124 0.0 30-31 0.0 0.0 0.0 0.0027540040021579587 0.0 32-33 0.0 0.0 0.0 0.005262114789837529 0.0 34-35 0.0 0.0 0.0 0.007253849827112481 0.0 36-37 0.0 0.0 0.0 0.008901334364117688 0.0 38-39 0.0 0.0 0.0 0.010794712115601285 0.0 40-41 0.0 0.0 0.0 0.013032340367354626 0.0 42-43 0.0 0.0 0.0 0.015835523012408263 0.0 44-45 0.0 0.0 0.0 0.01873706294325326 0.0 46-47 0.0 0.0 0.0 0.02294183691083371 0.0 48-49 0.0 0.0 0.0 0.028302308986462597 0.0 50-51 0.0 0.0 0.0 0.03693316081465406 0.0 52-53 0.0 0.0 0.0 0.054489936328411044 0.0 54-55 0.0 0.0 0.0 0.07684162952449662 0.0 56-57 0.0 0.0 0.0 0.1049472239393765 0.0 58-59 0.0 0.0 0.0 0.13637237674971464 0.0 60-61 0.0 0.0 0.0 0.1815183709279469 0.0 62-63 0.0 0.0 0.0 0.2162630821337433 0.0 64-65 0.0 0.0 0.0 0.24879475440923415 0.0 66-67 0.0 0.0 0.0 0.31201389985162803 0.0 68-69 0.0 0.0 0.0 0.395986432595998 0.0 70-71 0.0 0.0 0.0 0.46579551618641263 0.0 72-73 0.0 0.0 0.0 0.5289900723073586 0.0 74-75 0.0 0.0 0.0 0.6456172239344586 0.0 76-77 0.0 0.0 0.0 0.7359583909338188 0.0 78-79 0.0 0.0 0.0 0.8141278438164987 0.0 80-81 0.0 0.0 0.0 0.9120671111432411 0.0 82-83 0.0 0.0 0.0 1.0655044769777562 0.0 84-85 0.0 0.0 0.0 1.2303266986426202 0.0 86-87 0.0 0.0 0.0 1.4339754588736222 0.0 88-89 0.0 0.0 0.0 1.6908846893606433 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9905 0.0 29.110739 1 TACATGG 10165 0.0 27.48027 2 GTGTAAG 530 0.0 26.888365 1 ACATGGG 10315 0.0 26.2056 3 ATGGGAG 2345 0.0 22.486982 5 GATGTGC 1335 0.0 22.419249 8 ATTCGCG 585 0.0 21.929754 80-81 GTATCAA 14710 0.0 21.571623 1 CATGGGA 5335 0.0 21.193056 4 CATGGGG 5275 0.0 21.163937 4 GTGTAGG 1095 0.0 20.823135 1 GAGTACT 5925 0.0 20.726372 12-13 CGCGGAA 610 0.0 20.6362 82-83 AGTACTT 6290 0.0 20.618784 12-13 AGAGTAC 11520 0.0 20.28989 10-11 GTACTTT 6210 0.0 20.005644 14-15 AATTCGC 625 0.0 19.767965 78-79 GTAAGGT 565 0.0 19.338842 3 ATGGGGA 2930 0.0 18.807951 5 TACTTTT 6815 0.0 18.752485 14-15 >>END_MODULE