FastQCFastQC Report
Thu 26 May 2016
SRR936467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936467_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033403
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69180.3402178515522993No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66060.3248741149688478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62270.3062354093113859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37580.18481334000195732No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32010.15742083590906475No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26570.130667654173816No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG24580.12088110423757613No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG23770.11689763416302622No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG22800.11212730580214547No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22760.11193059123056276No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC20480.10071786065034821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG6500.025.57475
GTACATG96700.025.3025781
GTAAGGT5850.025.169994
TACATGG102050.023.6950022
ACATGGG102300.022.7536473
GAGTACT78800.022.66551212-13
GTACTTT83100.021.4082614-15
ATACCGT2951.3460522E-1020.9298676
GGTATCA132200.020.2689691
GTATCAA166150.019.873231
CATGGGG50100.019.8146214
AGTACTT82300.019.62379512-13
ACTTTTT94500.019.07608416-17
ATGGGAG23800.018.9584335
CGCCGGT3557.8216544E-1118.7303057
ACCGTCG3306.002665E-1018.7100358
TACCGTC3054.5747583E-918.686447
AGAGTAC138950.018.3570810-11
CCGTCGT2854.2824468E-818.3312879
GTATACC1300.007561363318.2707863
Produced by FastQC (version 0.11.5)