##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936467_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2033403 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.848236675169655 31.0 31.0 34.0 30.0 34.0 2 31.96378189665305 31.0 31.0 34.0 30.0 34.0 3 32.08813304593335 33.0 31.0 34.0 30.0 34.0 4 35.83742770124761 37.0 35.0 37.0 35.0 37.0 5 35.68941228079235 37.0 35.0 37.0 33.0 37.0 6 35.73101446196352 37.0 35.0 37.0 33.0 37.0 7 35.65426282935552 37.0 35.0 37.0 33.0 37.0 8 35.61633281744937 37.0 35.0 37.0 33.0 37.0 9 37.208950217935154 39.0 37.0 39.0 33.0 39.0 10-11 37.098986034740776 39.0 37.0 39.0 33.0 39.0 12-13 36.96622951770997 39.0 37.0 39.0 33.0 39.0 14-15 38.04483715230084 40.0 37.0 41.0 33.0 41.0 16-17 38.015438897257454 40.0 37.0 41.0 33.0 41.0 18-19 38.140822552145345 40.0 37.0 41.0 33.0 41.0 20-21 38.13581124843428 40.0 37.0 41.0 33.0 41.0 22-23 38.036031716290374 40.0 37.0 41.0 33.0 41.0 24-25 37.89746154598966 40.0 37.0 41.0 33.0 41.0 26-27 37.68409336466996 39.5 37.0 41.0 32.0 41.0 28-29 37.70129925056666 40.0 37.0 41.0 32.0 41.0 30-31 37.58408293879767 39.5 37.0 41.0 32.0 41.0 32-33 37.400547997617785 39.0 36.0 41.0 32.0 41.0 34-35 37.20731699520459 39.0 36.0 41.0 31.0 41.0 36-37 37.02374590772218 39.0 36.0 41.0 31.0 41.0 38-39 36.80504258132795 39.0 35.0 40.5 31.0 41.0 40-41 36.57116567645469 39.0 35.0 40.0 30.0 41.0 42-43 36.28610093523025 39.0 35.0 40.0 30.0 41.0 44-45 35.98868669909507 38.0 35.0 40.0 29.5 41.0 46-47 35.592706167936214 38.0 35.0 40.0 28.5 41.0 48-49 35.17282284918435 38.0 34.0 40.0 27.0 41.0 50-51 34.82868127960862 38.0 34.0 40.0 26.0 41.0 52-53 34.518890500309084 38.0 33.5 40.0 25.5 41.0 54-55 34.14608171621661 37.0 33.0 40.0 24.0 41.0 56-57 33.75906325504585 37.0 33.0 40.0 23.5 41.0 58-59 33.350765195094134 36.0 32.0 39.0 23.0 40.5 60-61 32.928204345129814 36.0 31.5 39.0 22.0 40.0 62-63 32.56153428513679 35.0 31.0 39.0 22.0 40.0 64-65 32.20999428052383 35.0 31.0 38.0 20.5 40.0 66-67 31.72681263871451 35.0 31.0 38.0 19.5 40.0 68-69 31.263759077762746 34.5 30.0 37.0 18.0 39.0 70-71 30.732714321755203 34.0 29.5 36.5 15.5 39.0 72-73 30.15851481482028 34.0 29.0 36.0 12.0 38.5 74-75 29.620740453318895 34.0 29.0 35.5 8.0 37.5 76-77 28.281128728540285 31.5 26.5 34.0 7.0 36.0 78-79 29.071904831457417 33.0 29.0 35.0 7.0 36.0 80-81 28.92649366603669 33.0 29.0 35.0 4.0 36.0 82-83 28.532506591167614 33.0 27.5 35.0 2.0 36.0 84-85 28.086252946415442 33.0 27.0 35.0 2.0 35.0 86-87 27.64201882263378 33.0 26.0 35.0 2.0 35.0 88-89 27.134310070359884 32.0 25.5 34.0 2.0 35.0 90-91 26.521739419092036 32.0 25.0 34.0 2.0 35.0 92-93 25.893557253530165 32.0 24.0 34.0 2.0 35.0 94-95 25.077743319941987 31.0 20.5 34.0 2.0 35.0 96-97 24.16530786076346 31.0 13.0 34.0 2.0 35.0 98-99 22.90519710062393 30.5 2.0 34.0 2.0 35.0 100-101 19.057252300699858 24.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2829995837180661 1101 2 0.40820340393844035 1101 3 0.23366961134336606 1101 4 0.19926628529248802 1101 5 0.19836153985406213 1101 6 0.25609147258467857 1101 7 0.27641871854081046 1101 8 0.21468715445212183 1101 9 0.18695314268673258 1101 10-11 0.2719159594295135 1101 12-13 0.3584101533359174 1101 14-15 0.392570852385127 1101 16-17 0.3857353622961597 1101 18-19 0.303716191800504 1101 20-21 0.2738829216547387 1101 22-23 0.21781219494762638 1101 24-25 0.30526904866889737 1101 26-27 0.1727712329783202 1101 28-29 0.2543624104072606 1101 30-31 0.4319554699240058 1101 32-33 0.33919312335607543 1101 34-35 0.3391844850662906 1101 36-37 0.3022396453599825 1101 38-39 0.4048510119818758 1101 40-41 0.19163667798945028 1101 42-43 0.37125292259499787 1101 44-45 0.30641973976399584 1101 46-47 0.11643921553397973 1101 48-49 0.2660230781841548 1101 50-51 0.16824147829782987 1101 52-53 0.2210100474319674 1101 54-55 0.09704461913143092 1101 56-57 0.08387049403424385 1101 58-59 0.17045806649437623 1101 60-61 0.06350837485188521 1101 62-63 0.051488731652888475 1101 64-65 0.1695937460340744 1101 66-67 0.005848829089792673 1101 68-69 0.21808148318775622 1101 70-71 0.014562748363925238 1101 72-73 -0.014526345716923572 1101 74-75 0.13339387485014953 1101 76-77 0.1588732436242246 1101 78-79 0.24294637183671952 1101 80-81 0.311712130988937 1101 82-83 0.11750939559628648 1101 84-85 -0.00822706953096386 1101 86-87 0.056822195871358616 1101 88-89 0.06432385870495594 1101 90-91 0.24738610129694294 1101 92-93 0.1741306366393367 1101 94-95 0.270695616080463 1101 96-97 0.39674590416038313 1101 98-99 -0.32346341491505015 1101 100-101 -0.13596321770820197 1102 1 0.2944604618897202 1102 2 0.37358978991382585 1102 3 0.3222298845534013 1102 4 0.1989369739325113 1102 5 0.06557641662017488 1102 6 0.12078584314645013 1102 7 0.1987634475322082 1102 8 0.1251046313531674 1102 9 0.18615826011703263 1102 10-11 0.3157570142212265 1102 12-13 0.33964713189114093 1102 14-15 0.4115545184614007 1102 16-17 0.41103715077191083 1102 18-19 0.28253731733495613 1102 20-21 0.2956936284279621 1102 22-23 0.2810757969516686 1102 24-25 0.19022529902259322 1102 26-27 0.4299044060106638 1102 28-29 0.37138594141971737 1102 30-31 0.46972553634415704 1102 32-33 0.39834936383644504 1102 34-35 0.4099819122234507 1102 36-37 0.29630371277470147 1102 38-39 0.19470882412630175 1102 40-41 0.28773489023613763 1102 42-43 0.3062739314958307 1102 44-45 0.43299189547509087 1102 46-47 0.19820295179110303 1102 48-49 0.2814257591518867 1102 50-51 0.3090047261065294 1102 52-53 0.43197190149707154 1102 54-55 0.3661714684224151 1102 56-57 0.44650130047983083 1102 58-59 0.2944312511981124 1102 60-61 0.3245756246063465 1102 62-63 0.0925643536620413 1102 64-65 0.16146852503826636 1102 66-67 0.23946343165051687 1102 68-69 0.2842936359609354 1102 70-71 0.0929100548538635 1102 72-73 -0.008970395496117334 1102 74-75 0.053924180525747545 1102 76-77 -0.04822737955889167 1102 78-79 0.05365374708670956 1102 80-81 0.22618940890645334 1102 82-83 0.2864194331911598 1102 84-85 0.1167950551353556 1102 86-87 0.16998349261561074 1102 88-89 0.1865751323030942 1102 90-91 0.040551275125576325 1102 92-93 0.31775504027673307 1102 94-95 0.24547740967749476 1102 96-97 0.1671955175155304 1102 98-99 0.18441216358727885 1102 100-101 0.09168555135763867 1103 1 0.2902865147364224 1103 2 0.35456801733816334 1103 3 0.3229268658363438 1103 4 0.19805934526492308 1103 5 0.29537577097450907 1103 6 0.15021369913197447 1103 7 0.2102365415931189 1103 8 0.25711121882878274 1103 9 0.27187226791227204 1103 10-11 0.3221979867949827 1103 12-13 0.1968393196659619 1103 14-15 0.39223606729633786 1103 16-17 0.3744395787521668 1103 18-19 0.31634071104421935 1103 20-21 0.41696529469958676 1103 22-23 0.2787571579927359 1103 24-25 0.2720420876361729 1103 26-27 0.2891228221976263 1103 28-29 0.4218412522344579 1103 30-31 0.5515839135589218 1103 32-33 0.5986889015141301 1103 34-35 0.4960132440234659 1103 36-37 0.5616081194989064 1103 38-39 0.29117403124150343 1103 40-41 0.4994908463392207 1103 42-43 0.6640803203784387 1103 44-45 0.7979644271469439 1103 46-47 0.612390580502364 1103 48-49 0.5969743574671398 1103 50-51 0.6395797128358964 1103 52-53 0.6048549225883519 1103 54-55 0.3812294205004392 1103 56-57 0.5080725793720049 1103 58-59 0.6134546317340153 1103 60-61 0.3743768330778039 1103 62-63 0.662272606150708 1103 64-65 0.4922721001889627 1103 66-67 0.5263080538572744 1103 68-69 0.5760389636453667 1103 70-71 0.42350548848500935 1103 72-73 0.643591978472557 1103 74-75 0.5055628905207854 1103 76-77 0.5783675284082115 1103 78-79 0.23740558681158674 1103 80-81 0.4289450557253289 1103 82-83 0.2976298937467057 1103 84-85 0.37847112936198 1103 86-87 0.42605036629226944 1103 88-89 0.3164500806399282 1103 90-91 0.30564237897638336 1103 92-93 0.26749466982651526 1103 94-95 0.24167825621893257 1103 96-97 0.5351302692683078 1103 98-99 0.32414192304040057 1103 100-101 0.20102704598248522 1104 1 0.19636623390844932 1104 2 0.22898620104755807 1104 3 0.11399676897002564 1104 4 0.14194125467890473 1104 5 0.13546976251068799 1104 6 0.06142175573656772 1104 7 0.042344201051527364 1104 8 0.12285549731106471 1104 9 0.1513970848392603 1104 10-11 0.1399001902232655 1104 12-13 0.1788532824379061 1104 14-15 0.21317936523952596 1104 16-17 0.2196223896691336 1104 18-19 0.20046456921822653 1104 20-21 0.19761410330199425 1104 22-23 0.078543965269958 1104 24-25 0.09468916645359826 1104 26-27 0.23149219353708617 1104 28-29 0.2370716322384112 1104 30-31 0.24101154474452358 1104 32-33 0.20441074684743654 1104 34-35 0.23198290234998353 1104 36-37 0.25691083596569086 1104 38-39 0.3456053357631035 1104 40-41 0.36707220233666504 1104 42-43 0.437367509731736 1104 44-45 0.4422935662431158 1104 46-47 0.3356753537260815 1104 48-49 0.22823858294199084 1104 50-51 0.30434009420762465 1104 52-53 0.2552035004708486 1104 54-55 0.22541056142139837 1104 56-57 0.3513767471545961 1104 58-59 0.4080590457980193 1104 60-61 0.16606182547313608 1104 62-63 0.23014189108282324 1104 64-65 0.4046618079683313 1104 66-67 0.5047029411632558 1104 68-69 0.3913094759713118 1104 70-71 0.10126792315736921 1104 72-73 0.3983464300889459 1104 74-75 0.6378076960469237 1104 76-77 0.24854290665534862 1104 78-79 0.3383328482065089 1104 80-81 0.29029607348920905 1104 82-83 0.18679711530414878 1104 84-85 0.31625026399445133 1104 86-87 0.43073561012888817 1104 88-89 0.368071508351381 1104 90-91 0.31511954491370986 1104 92-93 0.47626853729648033 1104 94-95 0.4945778164833463 1104 96-97 0.7464050356203877 1104 98-99 0.6967326528016287 1104 100-101 0.5069051616634965 1105 1 0.03133117614048686 1105 2 0.21039686858381756 1105 3 0.18329246937894084 1105 4 0.1139313199071168 1105 5 0.07669222650422824 1105 6 0.020862416075296153 1105 7 0.07645578041503143 1105 8 0.10994913453676247 1105 9 0.14851765236893755 1105 10-11 0.07119080148027734 1105 12-13 0.1741029486239043 1105 14-15 0.20905308221361452 1105 16-17 0.15636494880696716 1105 18-19 0.17445355690067998 1105 20-21 0.06817954794390602 1105 22-23 0.10019600472548262 1105 24-25 0.020719861495635428 1105 26-27 0.11543709525273727 1105 28-29 0.08676317225314278 1105 30-31 0.16943856979246164 1105 32-33 0.2795293227522748 1105 34-35 0.23332879203832135 1105 36-37 0.3068586684056527 1105 38-39 0.2802078156195904 1105 40-41 0.30499947800573324 1105 42-43 0.3422551222089183 1105 44-45 0.16562118102216772 1105 46-47 0.454045882682621 1105 48-49 0.3861976595553287 1105 50-51 0.5347559918441078 1105 52-53 0.5588604005585012 1105 54-55 0.5212302343045252 1105 56-57 0.5888190021276074 1105 58-59 0.5602536278778985 1105 60-61 0.3123668109571227 1105 62-63 0.39546405808407314 1105 64-65 0.3537102399807779 1105 66-67 0.3361195718978358 1105 68-69 0.41278616403599244 1105 70-71 0.3466589546739165 1105 72-73 0.4503344686637476 1105 74-75 0.5743215269139412 1105 76-77 0.38255598899502274 1105 78-79 0.4257258129669239 1105 80-81 0.539994994525621 1105 82-83 0.5326669958150028 1105 84-85 0.596213250464249 1105 86-87 0.8298011811238908 1105 88-89 0.8288914189284249 1105 90-91 0.7422234071479785 1105 92-93 0.8142409331093994 1105 94-95 0.978332896669599 1105 96-97 0.9497213244927281 1105 98-99 0.8488240256800026 1105 100-101 0.3740580305456902 1106 1 0.20529985822349772 1106 2 0.28621646404938517 1106 3 0.2244856656907146 1106 4 0.20783065225865016 1106 5 0.19689616029037182 1106 6 0.06944719178554237 1106 7 0.13120644109110913 1106 8 0.13929607706050007 1106 9 0.25604587352621877 1106 10-11 0.21562356387157422 1106 12-13 0.23873143949347764 1106 14-15 0.3430096221415795 1106 16-17 0.34928130228312426 1106 18-19 0.24036216082494377 1106 20-21 0.18084799058497936 1106 22-23 0.1611948160187069 1106 24-25 0.2584746086701202 1106 26-27 0.22839910085104975 1106 28-29 0.2809772823216008 1106 30-31 0.243261894634216 1106 32-33 0.17802753884182465 1106 34-35 0.13582635170323698 1106 36-37 0.22553365148486648 1106 38-39 0.23522678561112542 1106 40-41 0.009438549556016085 1106 42-43 0.1540578836138664 1106 44-45 0.1615172516575356 1106 46-47 0.03344690371201153 1106 48-49 0.11517740392385178 1106 50-51 0.11178342563194832 1106 52-53 0.07300945486488786 1106 54-55 0.09459015056787479 1106 56-57 0.15708354975580363 1106 58-59 0.09691179730347699 1106 60-61 0.11759257171245707 1106 62-63 0.2126906130111621 1106 64-65 0.19892497246222973 1106 66-67 -0.1884880613342581 1106 68-69 0.12898475969542744 1106 70-71 -0.09960095546733427 1106 72-73 -0.060173214565452326 1106 74-75 -0.10706870732421692 1106 76-77 -0.12285526273532099 1106 78-79 -0.12172042313506992 1106 80-81 -0.16972034506358824 1106 82-83 -0.2505863959956791 1106 84-85 -0.12880307512236655 1106 86-87 -0.10928482410709961 1106 88-89 -0.3005043728115062 1106 90-91 -0.2172189890399956 1106 92-93 -0.3853763509251955 1106 94-95 -0.23215769571111267 1106 96-97 0.08962501203460249 1106 98-99 0.08060887995713628 1106 100-101 0.06923318198251138 1107 1 0.0982944654659903 1107 2 0.1711842390350249 1107 3 0.12494290783867967 1107 4 0.04216431095732531 1107 5 0.08120801258834831 1107 6 0.06069521017214896 1107 7 0.05026575450101234 1107 8 0.09318765425169317 1107 9 0.015250424487433634 1107 10-11 0.12563451899192302 1107 12-13 0.09292017216085213 1107 14-15 0.11326534545643341 1107 16-17 0.1134238780690211 1107 18-19 0.14258727902948465 1107 20-21 0.03243596383579472 1107 22-23 0.1599128246443442 1107 24-25 0.25454383187766183 1107 26-27 0.21403197105797034 1107 28-29 0.2383275969568146 1107 30-31 0.18170683866445358 1107 32-33 0.1662719851323189 1107 34-35 0.07685820981625824 1107 36-37 0.19618889921250116 1107 38-39 0.1335410296905124 1107 40-41 0.22020390230051845 1107 42-43 0.3245229310687492 1107 44-45 0.26718483903890444 1107 46-47 0.22079330704043088 1107 48-49 0.2815866339156088 1107 50-51 0.08760947285838228 1107 52-53 0.10986616604430566 1107 54-55 0.14221939675866935 1107 56-57 0.25810646032187634 1107 58-59 0.1556194738487875 1107 60-61 0.055659365063924326 1107 62-63 -0.10603502785471619 1107 64-65 0.04505485553838895 1107 66-67 0.25725943092966475 1107 68-69 0.3163333263572454 1107 70-71 0.22158569347730506 1107 72-73 0.2755990608459271 1107 74-75 0.17127398891608436 1107 76-77 -0.012524393871398587 1107 78-79 0.3145832534933142 1107 80-81 0.44495882393947994 1107 82-83 0.4351846679489988 1107 84-85 0.35034931354384113 1107 86-87 0.6877443133978325 1107 88-89 0.5622758221307897 1107 90-91 0.610851386791591 1107 92-93 0.48634490096988614 1107 94-95 0.6327572766328338 1107 96-97 0.8005642967846605 1107 98-99 1.208877676728406 1107 100-101 0.8125047824338694 1108 1 -0.09865096704124454 1108 2 0.11433965059474005 1108 3 -0.02611486256312645 1108 4 0.029217898902544448 1108 5 0.08018265886774145 1108 6 0.022336058009301496 1108 7 0.018540570439448345 1108 8 0.07406974033560232 1108 9 0.1216220641196415 1108 10-11 -0.03748260917200952 1108 12-13 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-1.1757462160418655 2316 66-67 -1.3045591369900542 2316 68-69 -1.3250158460489665 2316 70-71 -1.0492530230024038 2316 72-73 -0.8357277473715001 2316 74-75 -0.977947199742033 2316 76-77 -1.0772702796625673 2316 78-79 -1.2209547938427363 2316 80-81 -1.4377743442612 2316 82-83 -1.320120886864057 2316 84-85 -1.1886198399136987 2316 86-87 -1.661246922097373 2316 88-89 -1.3144259946254202 2316 90-91 -1.6805509488256796 2316 92-93 -1.882131468131302 2316 94-95 -1.8155674882164838 2316 96-97 -1.7674396919411777 2316 98-99 -1.3261172297013744 2316 100-101 -0.7263084454046442 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 18.0 4 37.0 5 97.0 6 217.0 7 427.0 8 974.0 9 1853.0 10 2784.0 11 4202.0 12 5960.0 13 8332.0 14 10438.0 15 13584.0 16 17402.0 17 21718.0 18 19470.0 19 15257.0 20 14928.0 21 16066.0 22 17046.0 23 19433.0 24 22792.0 25 27142.0 26 32521.0 27 40092.0 28 49221.0 29 59828.0 30 73220.0 31 88609.0 32 109727.0 33 138393.0 34 180107.0 35 239566.0 36 315661.0 37 335252.0 38 127966.0 39 3058.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.6892946025438 18.367434265534776 14.508012079599444 23.435259052321978 2 16.733162961170493 20.06496176463066 37.543945591079265 25.65792968311958 3 20.144239068583886 23.319190415931608 28.88892932688926 27.64764118859524 4 13.933776827643593 16.269129933800432 34.82212278271287 34.9749704558431 5 14.959002687251882 36.5257236497838 32.59217510023095 15.923098562733365 6 34.14620712175599 34.475900743728616 16.046155139930452 15.33173699458494 7 30.756565358512834 31.756811252090095 19.032113701190127 18.454509688206944 8 28.330020271805466 32.731539722804456 18.573439311958595 20.365000693431483 9 27.16993491695198 16.264024526384844 19.29613524526877 37.2699053113944 10-11 26.69473291816723 25.3006905173249 25.589664222979902 22.41491234152797 12-13 27.54854792680054 22.528072398830925 26.447733184223686 23.475646490144847 14-15 23.86133026457017 24.079512440416693 25.008132960065506 27.05102433494763 16-17 23.83459199438945 27.48049179872341 25.339181920723902 23.345734286163236 18-19 23.68361454665095 25.484843693558673 27.778090614974264 23.05345114481611 20-21 24.346350505141412 24.448323022970005 27.814670174108176 23.390656297780406 22-23 23.858271685688607 24.453064391000776 27.968830491824644 23.719833431485974 24-25 24.234402129050707 24.810901180267773 27.86640762527881 23.088289065402705 26-27 23.880200649159043 25.095775548342676 27.858783318579718 23.16524048391856 28-29 23.219079758493283 25.406056159114705 28.060008822657213 23.3148552597348 30-31 23.36717496322649 24.819083177723144 28.0011045577515 23.812637301298864 32-33 23.48785295480231 24.9638032905563 28.005285899906262 23.54305785473513 34-35 24.425569500293477 24.819199132873855 27.422618507526984 23.332612859305684 36-37 24.351087093100826 24.444920935889307 27.676360781289688 23.52763118972018 38-39 23.709379556928255 24.687122689414526 27.957905970820907 23.645591782836316 40-41 23.718324335688582 24.821204852457885 27.957548985014036 23.502921826839497 42-43 23.916043953557967 24.999022560292048 27.13151322174701 23.953420264402975 44-45 23.797226184035804 25.02874637289136 27.0439433433335 24.130084099739342 46-47 24.21667028129692 25.435513766823398 26.673241851221817 23.674574100657864 48-49 24.644634021053317 25.386508990162348 26.319028859354326 23.649828129430013 50-51 24.03201268428969 25.536132791608463 26.461463330909616 23.970391193192228 52-53 24.541701296849105 25.688382454915192 25.97546461819916 23.79445163003654 54-55 23.965175576577266 25.321278321707897 26.309081757381293 24.404464344333544 56-57 24.23251357613728 25.731680145664658 26.086447569624276 23.949358708573783 58-59 24.83997573478623 25.122979732841667 26.017236957386125 24.019807574985975 60-61 24.32868474316033 25.291238727601208 26.979863923430436 23.40021260580803 62-63 23.930724843162814 25.51637074582282 26.69528378229845 23.857620628715917 64-65 23.83443833351448 25.604635345022185 26.375315799232098 24.185610522231233 66-67 23.996125671263847 25.713533274203677 26.077655259002906 24.21268579552957 68-69 23.754836190417176 25.4334559378607 26.570707599759412 24.241000271962708 70-71 24.00423548031168 25.588089703459822 26.348553176745483 24.059121639483017 72-73 24.13021063992133 25.386387941288064 26.48324699394079 24.00015442484981 74-75 23.82592957360295 26.154631176818476 25.812175781965642 24.207263467612933 76-77 24.092028142332612 25.740664057543654 26.025829924391825 24.14147787573191 78-79 24.180440544407322 25.6605780774393 26.127243137488275 24.031738240665103 80-81 24.062723077010087 26.181615024069878 25.92959256163657 23.826069337283464 82-83 24.194343994102326 25.923417741128773 25.88611463978286 23.996123624986048 84-85 23.9285853042472 25.875778205858964 25.78027140259757 24.415365087296266 86-87 24.04101033249893 25.693841319176354 25.878532893345596 24.386615454979125 88-89 23.83577994420065 25.948984856077907 25.463482310287866 24.751752889433583 90-91 23.92507341103021 25.861413057511314 25.884774745376042 24.328738786082436 92-93 24.11813451626864 25.60031179918952 25.979265845693828 24.302287838848017 94-95 23.82417676451864 25.456619460709668 26.001717817177138 24.717485957594555 96-97 23.836628307268615 25.457017802694992 26.150732762860233 24.55562112717616 98-99 23.789841697932175 25.61592925315397 25.595151153909896 24.999077895003964 100-101 23.446279337510653 25.602684422490647 25.83063214057907 25.12040409941963 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 400.0 1 311.5 2 302.5 3 360.0 4 293.0 5 290.0 6 482.5 7 970.0 8 1548.5 9 1924.0 10 1829.0 11 1569.0 12 1600.5 13 1784.0 14 2012.0 15 2228.5 16 2446.0 17 2607.5 18 2765.0 19 2941.5 20 3087.5 21 3337.5 22 3736.0 23 4291.5 24 4976.0 25 5816.5 26 6909.0 27 8184.5 28 9607.0 29 11525.5 30 13874.0 31 16724.5 32 20322.5 33 22598.0 34 24342.5 35 26340.0 36 29497.0 37 33708.5 38 36579.5 39 37797.5 40 39923.5 41 46434.0 42 53524.0 43 58277.5 44 61142.0 45 64900.5 46 68322.5 47 74904.5 48 90952.5 49 93954.0 50 84203.5 51 79689.5 52 78754.5 53 82059.0 54 84151.5 55 80376.5 56 74314.5 57 69018.0 58 65556.5 59 59986.5 60 51808.0 61 43765.0 62 36578.0 63 29506.5 64 23048.0 65 19049.5 66 15201.5 67 12401.0 68 9339.0 69 6214.5 70 4792.5 71 3891.0 72 3497.0 73 3132.0 74 2598.0 75 2173.0 76 1498.0 77 907.0 78 590.0 79 447.5 80 306.5 81 191.0 82 125.0 83 77.0 84 44.0 85 23.5 86 12.0 87 6.5 88 3.5 89 4.0 90 3.5 91 1.5 92 1.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010966837365736157 2 0.010179979079405311 3 0.005508008004315917 4 2.9507185737406703E-4 5 0.004868685646672106 6 0.0 7 1.4753592868703351E-4 8 0.00181960978714008 9 4.917864289567784E-5 10-11 0.0 12-13 0.0 14-15 5.163757504046173E-4 16-17 0.004499845824954522 18-19 0.0020409136801706304 20-21 5.901437147481341E-4 22-23 2.704825359262281E-4 24-25 4.1801846461326164E-4 26-27 1.4753592868703351E-4 28-29 9.835728579135568E-5 30-31 4.917864289567783E-4 32-33 0.003393326359801771 34-35 9.589835364657177E-4 36-37 0.0010573408222570736 38-39 0.004819507003776428 40-41 2.458932144783892E-5 42-43 0.0014507699654224963 44-45 0.005065400218254817 46-47 0.0 48-49 0.0020409136801706304 50-51 0.0010573408222570736 52-53 6.393223576438118E-4 54-55 1.2294660723919458E-4 56-57 0.01180287429496268 58-59 0.0019425563943792745 60-61 0.0030736651809798645 62-63 2.2130389303055026E-4 64-65 0.0034425050026974484 66-67 9.0980489357004E-4 68-69 0.001770431144244402 70-71 0.0050899895397026555 72-73 0.0025327001091274086 74-75 0.007155492541321125 76-77 0.001229466072391946 78-79 0.007180081862768964 80-81 0.0025818787520230863 82-83 0.00295071857374067 84-85 0.0019671457158271133 86-87 6.393223576438118E-4 88-89 0.0022622175732011805 90-91 0.007843993541860616 92-93 0.002114681644514147 94-95 4.1801846461326164E-4 96-97 1.4753592868703351E-4 98-99 5.901437147481341E-4 100-101 0.002065503001618469 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2033403.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.35934948172381 #Duplication Level Percentage of deduplicated Percentage of total 1 72.63200572126112 30.76644513905464 2 14.339857909903136 12.148541054476972 3 5.036331467580954 6.400071742231939 4 2.429157799797798 4.115901767515609 5 1.3637903869687586 2.8884636810712503 6 0.8505750329509436 2.161788304871866 7 0.5588026101057371 1.6569360536937856 8 0.40210897924806077 1.3626459825366266 9 0.2979934729763328 1.1360528698569392 >10 1.750118528882388 14.065190239538673 >50 0.184320282219632 5.5040462125585226 >100 0.14189137631963447 11.647147411915936 >500 0.009202393848749397 2.6427956025677544 >1k 0.0034945799425630617 2.529406052629355 >5k 3.494579942563062E-4 0.9745678854801293 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6918 0.3402178515522993 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6606 0.3248741149688478 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6227 0.3062354093113859 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3758 0.18481334000195732 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3201 0.15742083590906475 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2657 0.130667654173816 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2458 0.12088110423757613 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2377 0.11689763416302622 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2280 0.11212730580214547 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2276 0.11193059123056276 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2048 0.10071786065034821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.917864289567784E-5 0.0 10-11 0.0 0.0 0.0 4.917864289567784E-5 0.0 12-13 0.0 0.0 0.0 4.917864289567784E-5 0.0 14-15 0.0 0.0 0.0 4.917864289567784E-5 0.0 16-17 0.0 0.0 0.0 7.376796434351676E-5 0.0 18-19 0.0 0.0 0.0 9.835728579135568E-5 0.0 20-21 0.0 0.0 0.0 9.835728579135568E-5 0.0 22-23 0.0 0.0 0.0 2.2130389303055026E-4 0.0 24-25 0.0 0.0 0.0 2.704825359262281E-4 0.0 26-27 0.0 0.0 0.0 5.163757504046173E-4 0.0 28-29 0.0 0.0 0.0 0.0011311087866005902 0.0 30-31 0.0 0.0 0.0 0.002827771966501476 0.0 32-33 0.0 0.0 0.0 0.0054342400399724 0.0 34-35 0.0 0.0 0.0 0.007450564398695192 0.0 36-37 0.0 0.0 0.0 0.0090980489357004 0.0 38-39 0.0 0.0 0.0 0.011016016008631835 0.0 40-41 0.0 0.0 0.0 0.013401180189072211 0.0 42-43 0.0 0.0 0.0 0.016253541477021526 0.0 44-45 0.0 0.0 0.0 0.01905672412207516 0.0 46-47 0.0 0.0 0.0 0.023064783518072902 0.0 48-49 0.0 0.0 0.0 0.028376076950806114 0.0 50-51 0.0 0.0 0.0 0.03690857149320621 0.0 52-53 0.0 0.0 0.0 0.054440757685515366 0.0 54-55 0.0 0.0 0.0 0.07671868291725742 0.0 56-57 0.0 0.0 0.0 0.10472592004634595 0.0 58-59 0.0 0.0 0.0 0.13592976896365355 0.0 60-61 0.0 0.0 0.0 0.18068233399872036 0.0 62-63 0.0 0.0 0.0 0.21550081316886027 0.0 64-65 0.0 0.0 0.0 0.2477374135869771 0.0 66-67 0.0 0.0 0.0 0.3105139512433098 0.0 68-69 0.0 0.0 0.0 0.39416682280885784 0.0 70-71 0.0 0.0 0.0 0.4636562452204506 0.0 72-73 0.0 0.0 0.0 0.5268999799842924 0.0 74-75 0.0 0.0 0.0 0.6428632199323007 0.0 76-77 0.0 0.0 0.0 0.7337453520035133 0.0 78-79 0.0 0.0 0.0 0.8114476077786843 2.458932144783892E-5 80-81 0.0 0.0 0.0 0.9087721420692307 4.917864289567784E-5 82-83 0.0 0.0 0.0 1.0627012943327023 4.917864289567784E-5 84-85 0.0 0.0 0.0 1.2258760314605615 4.917864289567784E-5 86-87 0.0 0.0 0.0 1.4255167322955655 4.917864289567784E-5 88-89 0.0 0.0 0.0 1.6778523489932886 4.917864289567784E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 650 0.0 25.5747 5 GTACATG 9670 0.0 25.302578 1 GTAAGGT 585 0.0 25.16999 4 TACATGG 10205 0.0 23.695002 2 ACATGGG 10230 0.0 22.753647 3 GAGTACT 7880 0.0 22.665512 12-13 GTACTTT 8310 0.0 21.40826 14-15 ATACCGT 295 1.3460522E-10 20.929867 6 GGTATCA 13220 0.0 20.268969 1 GTATCAA 16615 0.0 19.87323 1 CATGGGG 5010 0.0 19.814621 4 AGTACTT 8230 0.0 19.623795 12-13 ACTTTTT 9450 0.0 19.076084 16-17 ATGGGAG 2380 0.0 18.958433 5 CGCCGGT 355 7.8216544E-11 18.730305 7 ACCGTCG 330 6.002665E-10 18.710035 8 TACCGTC 305 4.5747583E-9 18.68644 7 AGAGTAC 13895 0.0 18.35708 10-11 CCGTCGT 285 4.2824468E-8 18.331287 9 GTATACC 130 0.0075613633 18.270786 3 >>END_MODULE