FastQCFastQC Report
Thu 26 May 2016
SRR936466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101422
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65570.31202680851347325No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60270.2868057915068939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50960.2425024578594875No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37370.1778319633086548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31050.14775709019892244No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27500.13086376748696835No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23840.11344698970506636No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG23080.10983039103997197No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG22800.10849795995283194No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG22360.10640413967304045No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG22210.1056903373049297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG5050.026.3466071
GTACATG101100.026.3205471
GTACCGT2007.166818E-1026.1336486
TACATGG107250.024.19042
ACATGGG108950.023.0279033
GAGTACT60800.022.3067612-13
GTACTTT63500.021.47024314-15
GTATCAA158550.020.8293911
AGTACTT64350.020.11647812-13
GTATTAG6150.019.3162561
GATGTGC14550.019.2638768
AGAGTAC122950.019.04858610-11
TATAACG1250.006020419319.0067422
TAAGGTG7250.019.0062895
GTCTTAG6800.018.8674351
ACTTTTT73800.018.85970516-17
CATGGGG54400.018.6922914
TACTTTT69450.018.39961814-15
TCAACGC181200.018.2515074
ATCAACG182450.018.100423
Produced by FastQC (version 0.11.5)