##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936466_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2101422 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.257241525024483 31.0 31.0 34.0 28.0 34.0 2 31.299508142581548 31.0 31.0 34.0 28.0 34.0 3 31.34468041164507 31.0 31.0 34.0 28.0 34.0 4 34.99329834749993 37.0 35.0 37.0 32.0 37.0 5 34.897402806290216 37.0 35.0 37.0 32.0 37.0 6 35.02351074653259 37.0 35.0 37.0 32.0 37.0 7 34.84404798274692 37.0 35.0 37.0 32.0 37.0 8 34.8272441232651 37.0 35.0 37.0 32.0 37.0 9 36.309052631979675 39.0 35.0 39.0 32.0 39.0 10-11 36.172818691343295 39.0 35.0 39.0 31.5 39.0 12-13 36.06446872641478 39.0 35.0 39.0 30.5 39.0 14-15 37.11191255254775 39.0 36.0 41.0 31.0 41.0 16-17 37.04127704954074 39.0 36.0 41.0 31.0 41.0 18-19 36.97076669988227 39.0 36.0 41.0 31.0 41.0 20-21 36.85849272540213 39.0 36.0 41.0 30.0 41.0 22-23 36.74794448711397 39.0 36.0 41.0 30.0 41.0 24-25 36.57969294125597 39.0 36.0 40.5 30.0 41.0 26-27 36.36926424107104 39.0 35.5 40.0 29.5 41.0 28-29 36.47615043527668 39.0 36.0 40.5 30.0 41.0 30-31 36.416821561780544 39.0 35.5 40.0 30.0 41.0 32-33 36.28055859318118 39.0 35.0 40.0 30.0 41.0 34-35 36.0956302446629 38.5 35.0 40.0 29.5 41.0 36-37 35.85308710006843 38.0 35.0 40.0 28.5 41.0 38-39 35.66232579653206 38.0 35.0 40.0 27.0 41.0 40-41 35.49840346203666 38.0 34.5 40.0 27.0 41.0 42-43 35.2168234176667 38.0 34.0 40.0 26.0 41.0 44-45 34.94163190449134 38.0 34.0 40.0 25.5 41.0 46-47 34.57418643185424 38.0 33.0 40.0 24.0 41.0 48-49 34.305201192335474 38.0 33.0 40.0 24.0 41.0 50-51 33.57295726417635 37.0 32.0 39.5 23.0 40.5 52-53 33.746319634990016 37.5 33.0 39.5 23.0 40.5 54-55 34.06177578801402 38.0 33.0 40.0 23.0 41.0 56-57 33.951031492008745 37.5 33.0 40.0 23.0 41.0 58-59 33.68661553938238 37.0 33.0 40.0 22.5 41.0 60-61 33.33268520078309 36.5 32.5 39.5 22.0 41.0 62-63 32.98412979401567 36.0 32.0 39.0 21.0 41.0 64-65 32.646022550444414 35.5 32.0 39.0 20.0 41.0 66-67 32.25912881848576 35.0 31.0 38.5 19.5 40.0 68-69 31.873028120958093 35.0 31.0 38.0 18.0 40.0 70-71 31.46254821734997 35.0 31.0 37.0 17.5 39.5 72-73 31.059994137303214 35.0 30.5 37.0 14.0 39.0 74-75 30.643252283453776 34.5 30.0 36.0 12.0 39.0 76-77 30.24223668544443 34.0 30.0 36.0 7.5 38.0 78-79 29.808912488781406 34.0 29.0 35.0 7.0 37.0 80-81 29.409261680899885 34.0 29.0 35.0 4.0 37.0 82-83 29.03426632061528 34.0 29.0 35.0 2.0 36.0 84-85 28.673088270704312 34.0 29.0 35.0 2.0 36.0 86-87 28.347381439805996 34.0 27.5 35.0 2.0 35.5 88-89 27.975997443635784 33.5 27.0 35.0 2.0 35.0 90-91 27.63271632256634 33.0 26.5 35.0 2.0 35.0 92-93 27.266141450884213 33.0 26.0 35.0 2.0 35.0 94-95 26.823543295920572 33.0 25.0 35.0 2.0 35.0 96-97 26.208617545642902 33.0 24.5 35.0 2.0 35.0 98-99 25.504661367397887 32.5 21.5 35.0 2.0 35.0 100-101 23.361172577426142 30.5 9.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4647537065126919 1101 2 0.5282640036100084 1101 3 0.5346613690627464 1101 4 0.5462169360524953 1101 5 0.5747462824489062 1101 6 0.5436344990136419 1101 7 0.571013771678679 1101 8 0.4878457249679258 1101 9 0.7993348839618477 1101 10-11 0.6253655277116152 1101 12-13 0.8147204057630404 1101 14-15 0.8342653021403947 1101 16-17 0.8663372725653744 1101 18-19 0.8850029144140095 1101 20-21 0.9442025210525671 1101 22-23 0.8562469288153665 1101 24-25 0.9114329541484452 1101 26-27 0.7781225368917788 1101 28-29 0.8875348964015188 1101 30-31 0.6951112069788437 1101 32-33 0.7112938401728144 1101 34-35 0.7457477460797506 1101 36-37 0.9148495120745821 1101 38-39 0.7192420250945517 1101 40-41 0.7228917596130628 1101 42-43 0.4794014564226856 1101 44-45 0.5641422257609321 1101 46-47 0.6418243651585769 1101 48-49 0.6190173057510009 1101 50-51 0.7320840376604139 1101 52-53 0.6868208329824199 1101 54-55 0.8555526600165706 1101 56-57 0.6327987073276589 1101 58-59 0.6150093915117267 1101 60-61 0.5242078276620745 1101 62-63 0.5417314953473067 1101 64-65 0.5253743286653574 1101 66-67 0.5038670587203669 1101 68-69 0.5075810325448487 1101 70-71 0.3190421354070665 1101 72-73 0.43363228481839755 1101 74-75 0.4417864999681953 1101 76-77 0.4545741587411989 1101 78-79 0.5103240028385336 1101 80-81 0.4766858292068683 1101 82-83 0.5006547239884114 1101 84-85 0.4455618780938728 1101 86-87 0.39441122667114925 1101 88-89 0.47550179543372195 1101 90-91 0.6506776487947477 1101 92-93 0.25474080783505215 1101 94-95 0.2308249913517102 1101 96-97 0.21653844280100643 1101 98-99 0.21925807685204646 1101 100-101 0.4641302923919888 1102 1 0.21338988497848277 1102 2 0.30131089685031753 1102 3 0.3766621612707688 1102 4 0.33039982661346556 1102 5 0.30972089797843694 1102 6 0.3925864335630891 1102 7 0.16512671606793106 1102 8 0.3066450467605719 1102 9 0.5018152840590986 1102 10-11 0.5786735597670614 1102 12-13 0.5481379782895246 1102 14-15 0.6461859396424217 1102 16-17 0.5156940463465034 1102 18-19 0.39186086765118944 1102 20-21 0.25920463843782215 1102 22-23 0.06103209874559212 1102 24-25 0.06481638283120361 1102 26-27 0.3606019386781085 1102 28-29 0.4420742725216016 1102 30-31 0.3100375251920582 1102 32-33 0.24267441561855208 1102 34-35 0.25858268341963964 1102 36-37 0.1658196448660334 1102 38-39 0.19310016672266528 1102 40-41 -0.062281390975698514 1102 42-43 0.3283024690642051 1102 44-45 0.37421820205265277 1102 46-47 0.6015278265601793 1102 48-49 0.6632583801267771 1102 50-51 0.695792014003672 1102 52-53 0.6168038133913711 1102 54-55 0.44104148618004047 1102 56-57 0.6100607671651304 1102 58-59 0.5376988652891583 1102 60-61 0.07363086030523647 1102 62-63 0.22663699394725967 1102 64-65 0.30556719589483805 1102 66-67 0.4107479972614527 1102 68-69 0.43125814980934507 1102 70-71 0.4868755108734142 1102 72-73 0.28040343331070616 1102 74-75 0.2680375284699714 1102 76-77 0.3280684401908118 1102 78-79 0.27916973803817413 1102 80-81 0.3030064979463738 1102 82-83 0.25773528445948557 1102 84-85 0.11394130548205084 1102 86-87 0.10338145285297884 1102 88-89 -0.13953663148589612 1102 90-91 -0.3042429774360329 1102 92-93 -0.3686538437946929 1102 94-95 -0.15930585983716483 1102 96-97 -0.342201084997928 1102 98-99 -0.4010756246755314 1102 100-101 -0.1934001520656956 1103 1 0.20012527516562173 1103 2 0.311544300143602 1103 3 0.2861199794907172 1103 4 0.26118095213914216 1103 5 0.32183437708513907 1103 6 0.26524484902502365 1103 7 0.2418655025210512 1103 8 0.021074638410453872 1103 9 0.5382973628869081 1103 10-11 0.45526780927836086 1103 12-13 0.39183540072710343 1103 14-15 0.48315578173952645 1103 16-17 0.665410880713587 1103 18-19 0.52681020537932 1103 20-21 0.4899175256238877 1103 22-23 0.351296827506026 1103 24-25 0.45751413706988586 1103 26-27 0.5095352450917332 1103 28-29 0.4841729066592322 1103 30-31 0.3703210507978696 1103 32-33 0.5451797878226969 1103 34-35 0.5320785445139293 1103 36-37 0.5517645815342789 1103 38-39 0.40956184447805555 1103 40-41 0.26887293253349753 1103 42-43 0.33261138371462806 1103 44-45 0.31839521011448113 1103 46-47 0.4072725635217225 1103 48-49 0.6727004299689412 1103 50-51 0.09889650630885427 1103 52-53 0.47195033018410726 1103 54-55 0.7189695135129384 1103 56-57 0.7071053505904104 1103 58-59 0.6062778096921306 1103 60-61 0.7526365760487295 1103 62-63 0.6298678680396961 1103 64-65 0.6824709438386805 1103 66-67 0.5622750648753154 1103 68-69 0.48970483547631716 1103 70-71 0.5566570386296696 1103 72-73 0.3946789800390995 1103 74-75 0.29398707435634464 1103 76-77 0.3447293159892091 1103 78-79 0.39692880505104 1103 80-81 0.5826661122745485 1103 82-83 0.6666419920065536 1103 84-85 0.7033662018333366 1103 86-87 0.6402078535779303 1103 88-89 0.6438101617034206 1103 90-91 0.7347933438288194 1103 92-93 0.6459647727512312 1103 94-95 0.5234357484904137 1103 96-97 0.7591936925779557 1103 98-99 0.7615579761333109 1103 100-101 0.6799396686052042 1104 1 0.2666887632402499 1104 2 0.2775711531699514 1104 3 0.30535845614010526 1104 4 0.25972938586455996 1104 5 0.2659460363800079 1104 6 0.13903114245567139 1104 7 0.352521123455972 1104 8 0.4574089644008552 1104 9 0.4870229971066422 1104 10-11 0.4616299509407398 1104 12-13 0.5108204326133148 1104 14-15 0.5270671811124856 1104 16-17 0.1887242659325281 1104 18-19 0.47184880047894495 1104 20-21 0.428769963901928 1104 22-23 0.4586094012762416 1104 24-25 0.43783308656289677 1104 26-27 0.19583357634136433 1104 28-29 0.2877877853465165 1104 30-31 0.3117756524073201 1104 32-33 0.07580361941821678 1104 34-35 0.17484992681454514 1104 36-37 0.2775660486001357 1104 38-39 0.3173529108683155 1104 40-41 0.16787282958284777 1104 42-43 0.18802858270456824 1104 44-45 0.1145181491543994 1104 46-47 0.3884794149449249 1104 48-49 0.32129279817215206 1104 50-51 -0.017809460156584578 1104 52-53 0.3726435945898885 1104 54-55 0.608340769917433 1104 56-57 0.6363746402574719 1104 58-59 0.5098151334841035 1104 60-61 0.38357765522234644 1104 62-63 0.23661221876485428 1104 64-65 0.3195996044278431 1104 66-67 0.354284156444038 1104 68-69 0.5159460462064374 1104 70-71 0.4267528739146975 1104 72-73 0.018655029385516997 1104 74-75 -0.14622661093907396 1104 76-77 0.13690745937478255 1104 78-79 0.20414771651689279 1104 80-81 0.19712482925103458 1104 82-83 0.14074847717614958 1104 84-85 0.0805630681824887 1104 86-87 0.05934676060665467 1104 88-89 0.03285556598229178 1104 90-91 -0.07481717598082582 1104 92-93 -0.23690815080809813 1104 94-95 -0.2134693838087749 1104 96-97 -0.02561986986048126 1104 98-99 0.1143372069969324 1104 100-101 0.4654706565630171 1105 1 0.12405241150280943 1105 2 0.2167941006388503 1105 3 0.07856121359265344 1105 4 0.254516713398651 1105 5 0.269708362388954 1105 6 0.2884848213664526 1105 7 0.4119885390118725 1105 8 -0.06670231356798695 1105 9 0.342659790615194 1105 10-11 0.34286538774546926 1105 12-13 0.2079649933796972 1105 14-15 0.32958935607688034 1105 16-17 0.2693558040835953 1105 18-19 0.13199687218298806 1105 20-21 -0.2730023334041718 1105 22-23 -0.2711136541328827 1105 24-25 -0.1984803099052428 1105 26-27 -0.21749221597106327 1105 28-29 -0.09170597026888316 1105 30-31 -0.13259046270965769 1105 32-33 -0.27525756616399377 1105 34-35 -0.25051408105989736 1105 36-37 -0.3039714130801343 1105 38-39 -0.3457830180796222 1105 40-41 -0.43448510247506533 1105 42-43 -0.2568818609042651 1105 44-45 -0.14940089888646213 1105 46-47 -0.14441610099330404 1105 48-49 0.05119509290770452 1105 50-51 -0.08775907105236769 1105 52-53 0.06206254375636888 1105 54-55 0.11472690279968845 1105 56-57 0.1506842279371412 1105 58-59 -0.07377479791199448 1105 60-61 0.0944794481255613 1105 62-63 -0.12335427160259371 1105 64-65 0.052913206955096825 1105 66-67 -0.3512561330246413 1105 68-69 -0.15341018619425384 1105 70-71 -0.021208037935625867 1105 72-73 0.04056642320831472 1105 74-75 0.024750896886356344 1105 76-77 -0.05076178155139388 1105 78-79 -0.06894313440150412 1105 80-81 0.11385050261201002 1105 82-83 -0.05205222134726739 1105 84-85 -0.2673653954947284 1105 86-87 0.002030122532023171 1105 88-89 0.03705352476933399 1105 90-91 0.005347565547186406 1105 92-93 -0.11955559309690855 1105 94-95 -0.34435082766560043 1105 96-97 -0.08701906605992704 1105 98-99 -0.11463224997968169 1105 100-101 0.00847156862487708 1106 1 0.10821375049777515 1106 2 0.041491138439383235 1106 3 -0.0027156785629998126 1106 4 0.10228882945781237 1106 5 0.3353314278817976 1106 6 0.1437337538908423 1106 7 0.41681981848177685 1106 8 0.41731944494747353 1106 9 0.5047715382506297 1106 10-11 0.34963551836300866 1106 12-13 0.32841082221469264 1106 14-15 0.3635972520166746 1106 16-17 0.2907652728719867 1106 18-19 0.18980429007395827 1106 20-21 0.3093820372482554 1106 22-23 0.23469230188647572 1106 24-25 -0.039900941313099736 1106 26-27 0.17855966137683055 1106 28-29 0.0654542028185503 1106 30-31 0.12219680403723032 1106 32-33 0.0869162575506337 1106 34-35 0.01607069584620291 1106 36-37 0.1596593749748294 1106 38-39 0.05734001399791566 1106 40-41 -0.16915769555194515 1106 42-43 -1.548837591869301E-4 1106 44-45 -0.06828993756075619 1106 46-47 -0.07982279955789551 1106 48-49 -0.138141531291609 1106 50-51 0.15434222045850277 1106 52-53 -0.09532676281315844 1106 54-55 0.21710316976464128 1106 56-57 0.08412963936731188 1106 58-59 0.10405006467473754 1106 60-61 0.017640489628888645 1106 62-63 0.09080734235255505 1106 64-65 -0.07184033134322476 1106 66-67 -0.05621059083562585 1106 68-69 -0.06534779238329946 1106 70-71 0.1667601820141833 1106 72-73 0.11998924360470298 1106 74-75 0.006751029783774953 1106 76-77 -0.20234732796409816 1106 78-79 0.04480875069874557 1106 80-81 0.046972072054156655 1106 82-83 0.0010184346351564955 1106 84-85 0.1681192084537777 1106 86-87 0.3318693930321217 1106 88-89 0.2556143106360764 1106 90-91 0.2989172971669589 1106 92-93 0.1787347656153777 1106 94-95 0.19270807606053353 1106 96-97 0.11190001583276299 1106 98-99 0.21804445680698237 1106 100-101 0.5130976781560257 1107 1 0.6160472890525313 1107 2 0.5751934766918225 1107 3 0.6079272057343594 1107 4 0.5959870708517983 1107 5 0.647263596259954 1107 6 0.6480563483377395 1107 7 0.6811253260312142 1107 8 0.6337182791141842 1107 9 0.6958080025618614 1107 10-11 0.728611276497567 1107 12-13 0.7812650426374859 1107 14-15 0.8840818849367906 1107 16-17 0.8380524213177054 1107 18-19 1.055624567888195 1107 20-21 1.0964407790221742 1107 22-23 1.01896602017613 1107 24-25 1.1140792793086334 1107 26-27 1.1939816991142607 1107 28-29 0.9512935614558131 1107 30-31 0.9509183707267752 1107 32-33 1.0483622986999919 1107 34-35 0.9754983983307071 1107 36-37 1.0871611043305478 1107 38-39 1.2358325744754026 1107 40-41 1.0997497377026448 1107 42-43 1.0815657468299946 1107 44-45 1.0222445026207936 1107 46-47 1.1677198802144844 1107 48-49 1.2286633324333422 1107 50-51 1.072304673795422 1107 52-53 1.1457218058526024 1107 54-55 1.2312373314324887 1107 56-57 1.1072960794652928 1107 58-59 1.1696569152397842 1107 60-61 1.0870686145167596 1107 62-63 1.2165274151876133 1107 64-65 1.1636955327464449 1107 66-67 1.0794863769895002 1107 68-69 1.1362911099674875 1107 70-71 1.110923793826398 1107 72-73 1.335841905677018 1107 74-75 1.1559717182830944 1107 76-77 1.1321109760545944 1107 78-79 1.1007759476708863 1107 80-81 1.105603816711774 1107 82-83 1.1642794930871538 1107 84-85 1.2085520674387276 1107 86-87 1.3777311572156776 1107 88-89 1.2881465234347154 1107 90-91 1.158309322463925 1107 92-93 1.2210078044260726 1107 94-95 1.4910465774006383 1107 96-97 1.6983844934174535 1107 98-99 1.9151275344651602 1107 100-101 2.0827580113459874 1108 1 0.1652263502515794 1108 2 0.09269805540013465 1108 3 -0.01658051072313782 1108 4 0.18532222342734883 1108 5 0.20169113510472414 1108 6 0.2925777830514349 1108 7 0.2650961622902983 1108 8 0.34996578642618914 1108 9 0.3986754040071787 1108 10-11 0.18267508191620863 1108 12-13 0.2254476862090513 1108 14-15 0.42078918797346887 1108 16-17 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-4.181262868145193 2316 98-99 -4.657733797285669 2316 100-101 -5.245575916797517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4880.0 3 2111.0 4 2192.0 5 2508.0 6 2832.0 7 3448.0 8 4106.0 9 4939.0 10 6124.0 11 7595.0 12 9044.0 13 10453.0 14 11606.0 15 13669.0 16 16095.0 17 19522.0 18 17817.0 19 14291.0 20 15002.0 21 16234.0 22 17924.0 23 20577.0 24 23341.0 25 27510.0 26 32134.0 27 38277.0 28 44900.0 29 54049.0 30 65392.0 31 80238.0 32 99521.0 33 125979.0 34 163893.0 35 221148.0 36 297762.0 37 351786.0 38 226509.0 39 26010.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.23226394763995 17.474956499887682 13.814474400612237 23.47830515186013 2 16.25281017583468 19.407345575959933 38.592161394037575 25.74768285416782 3 20.05940333014631 23.2463953238795 28.315000411663842 28.37920093431035 4 13.327084509549847 15.556096125887933 35.25631443802001 35.860504926542205 5 15.162231439268425 36.40433315432459 32.37025674424982 16.06317866215717 6 34.068000870401995 33.95296305695848 16.58958593529409 15.389450137345436 7 30.93011688042782 31.435146951374794 19.158537078774696 18.47619908942269 8 28.21983480594006 32.55926085820376 18.794120488947 20.426783846909192 9 27.47250969014385 16.455539563962564 19.30999131884952 36.76195942704407 10-11 26.680861800965015 25.14714458803562 25.720226519113442 22.45176709188592 12-13 27.60719913545629 22.433338252726692 26.404217030939808 23.55524558087721 14-15 24.015801905033882 24.001047114188992 24.88562062338991 27.09753035738721 16-17 23.919850999815797 27.481380926700798 25.121291119153028 23.477476954330378 18-19 23.785992872648997 25.69492624288925 27.3269149251909 23.192165959270852 20-21 24.575989833037628 24.74153615852084 27.09457393121355 23.58790007722798 22-23 24.053800906850974 24.653434339924907 27.39849803699651 23.89426671622761 24-25 24.3864310280091 25.00515144290369 27.1820584166487 23.42635911243851 26-27 23.876425614859613 25.300969746461128 27.396455842701013 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24.462852785223202 25.812261097053042 25.739547754416396 23.985338363307363 54-55 23.93201460195121 25.41923979816909 26.05044916085417 24.59829643902553 56-57 24.160924195092306 25.813223678121155 25.8485576940236 24.177294432762935 58-59 24.6444879663058 25.232461386509424 25.84515676792836 24.277893879256418 60-61 24.282991617513044 25.326626709273082 26.697875944903526 23.692505728310344 62-63 23.909859771556242 25.652989624736932 26.399018745903014 24.038131857803812 64-65 23.79580474756365 25.762348659898166 26.029462467369214 24.412384125168966 66-67 23.918871973997916 25.93791193364329 25.711749953601696 24.431466138757095 68-69 23.64316727673089 25.652964631345203 26.303448633607633 24.400419458316268 70-71 23.98152499681732 25.741504627674196 26.009563503064292 24.267406872444187 72-73 24.041768734838488 25.564469211797753 26.20953609197973 24.184225961384026 74-75 23.686563026819694 26.33612479611297 25.716784111147728 24.26052806591961 76-77 24.047629131786493 25.94045002262819 25.71911727685057 24.29280356873474 78-79 24.15325139373328 25.905111410594706 25.830396548120987 24.111240647551025 80-81 24.020140637951876 26.428816048538582 25.70808640854404 23.8429569049655 82-83 24.141265534480844 26.08900491045329 25.70907949642453 24.060650058641333 84-85 23.93000807855352 26.073953863012004 25.534487333089828 24.461550725344647 86-87 24.14878737447454 25.866965026338875 25.657900685633955 24.326346913552634 88-89 23.835854517298742 26.092582453340075 25.291409652478507 24.78015337688268 90-91 23.962863547452134 25.95703822156227 25.76167087860573 24.318427352379864 92-93 24.19424079683065 25.787602254793146 25.85989689328188 24.15826005509432 94-95 23.875850791756257 25.683203269629246 25.850601395912392 24.59034454270211 96-97 23.835136833544272 25.708164422493272 25.974448093719673 24.482250650242786 98-99 23.759757195496608 25.96813964352784 25.452182453695343 24.819920707280207 100-101 23.545612342930703 25.69100016853082 25.87509866670221 24.888288821836262 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 369.0 1 266.0 2 238.0 3 321.5 4 317.0 5 343.0 6 532.5 7 969.0 8 1555.5 9 1936.0 10 1808.0 11 1588.0 12 1688.0 13 1904.0 14 2015.0 15 2101.0 16 2164.5 17 2252.0 18 2372.5 19 2463.5 20 2613.0 21 2987.5 22 3557.5 23 4061.0 24 4618.0 25 5475.5 26 6620.0 27 8033.0 28 9545.5 29 11558.5 30 13932.0 31 16734.5 32 20670.0 33 23055.0 34 25022.0 35 27592.5 36 30900.5 37 34808.0 38 37864.0 39 39367.5 40 41150.0 41 47912.5 42 54896.0 43 60018.5 44 63512.0 45 67402.5 46 70659.5 47 76779.5 48 92893.0 49 96210.5 50 86743.5 51 82400.0 52 81686.5 53 84801.0 54 86980.5 55 83657.0 56 77917.0 57 72327.0 58 67726.0 59 62132.0 60 54082.0 61 45614.5 62 38701.5 63 31336.0 64 24796.5 65 20631.0 66 16287.0 67 13426.5 68 10127.5 69 6840.5 70 5417.0 71 4359.0 72 3649.5 73 3265.5 74 2714.5 75 2168.5 76 1483.0 77 885.0 78 583.0 79 405.5 80 274.0 81 176.5 82 113.5 83 68.5 84 47.0 85 32.5 86 19.0 87 23.0 88 19.0 89 9.0 90 5.5 91 3.5 92 2.0 93 2.5 94 2.5 95 4.0 96 4.0 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013229137222318981 2 0.00618628719029305 3 0.009184257136358143 4 0.036165986650943976 5 0.04596887250633143 6 0.05915042290410969 7 0.026505861269178678 8 0.023745825445817166 9 0.015132610203947612 10-11 0.04458885459465067 12-13 0.04244744749031846 14-15 0.019486804649423106 16-17 0.023079609902247146 18-19 0.030526947942869163 20-21 0.023769618858087524 22-23 0.014513981484918307 24-25 0.0032834908933093874 26-27 0.002284167577954357 28-29 0.0015703652098436201 30-31 0.0069476763829445015 32-33 0.001427604736221473 34-35 0.0017369190957361254 36-37 0.005686625532615533 38-39 0.006543188374348417 40-41 0.003545218428283324 42-43 6.424221312996628E-4 44-45 0.0013086376748696834 46-47 0.002450721463846862 48-49 5.472484822182312E-4 50-51 0.001594158622113978 52-53 9.279430785439574E-4 54-55 0.00280762264790223 56-57 0.0022603741656839985 58-59 0.002450721463846862 60-61 8.08976017192168E-4 62-63 0.001403811323951115 64-65 0.0033548711301204614 66-67 0.0024745148761172197 68-69 0.006685948847970565 70-71 0.009731505618576374 72-73 0.004259020796394061 74-75 0.003545218428283324 76-77 0.002902796296983661 78-79 0.036594268071810425 80-81 0.02357927155992466 82-83 0.004211433971853345 84-85 0.008541835005058479 86-87 0.007375957803810943 88-89 0.00606732012894126 90-91 0.011991879784260372 92-93 0.014180873713133296 94-95 0.006852502733863069 96-97 0.004354194445475492 98-99 0.0224847745954882 100-101 0.04358953127929564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2101422.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.892495465301096 #Duplication Level Percentage of deduplicated Percentage of total 1 74.84182287177383 33.598361938859746 2 13.192743873232407 11.845103890079292 3 4.695734843114201 6.324097654522818 4 2.205129174978615 3.9597500595252253 5 1.2488897288977274 2.803288824560117 6 0.7743713100402063 2.085807631466354 7 0.5043160873749 1.5848005365889262 8 0.372099989672748 1.3363597679217938 9 0.273018040925 1.1030815047753855 >10 1.585419072292172 13.460697757827706 >50 0.16603980775904512 5.2472921947586455 >100 0.12918772698993958 11.17774428340843 >500 0.008019623535155902 2.3959151758546793 >1k 0.0028870644726561247 2.229009480501924 >5k 3.2078494140623607E-4 0.848689299349069 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6557 0.31202680851347325 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6027 0.2868057915068939 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5096 0.2425024578594875 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3737 0.1778319633086548 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3105 0.14775709019892244 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2750 0.13086376748696835 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2384 0.11344698970506636 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2308 0.10983039103997197 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2280 0.10849795995283194 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2236 0.10640413967304045 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2221 0.1056903373049297 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.758682454071576E-5 0.0 5 0.0 0.0 0.0 4.758682454071576E-5 0.0 6 0.0 0.0 0.0 4.758682454071576E-5 0.0 7 0.0 0.0 0.0 4.758682454071576E-5 0.0 8 0.0 0.0 0.0 4.758682454071576E-5 0.0 9 0.0 0.0 0.0 4.758682454071576E-5 0.0 10-11 0.0 0.0 0.0 4.758682454071576E-5 0.0 12-13 0.0 0.0 0.0 4.758682454071576E-5 0.0 14-15 0.0 0.0 0.0 4.758682454071576E-5 0.0 16-17 0.0 0.0 0.0 4.758682454071576E-5 0.0 18-19 0.0 0.0 0.0 9.517364908143153E-5 4.758682454071576E-5 20-21 0.0 0.0 0.0 1.427604736221473E-4 4.758682454071576E-5 22-23 0.0 0.0 0.0 1.9034729816286305E-4 4.758682454071576E-5 24-25 0.0 0.0 0.0 1.9034729816286305E-4 4.758682454071576E-5 26-27 0.0 0.0 0.0 3.806945963257261E-4 4.758682454071576E-5 28-29 0.0 0.0 0.0 0.0011182903767068203 4.758682454071576E-5 30-31 0.0 0.0 0.0 0.002617275349739367 4.758682454071576E-5 32-33 0.0 0.0 0.0 0.00471109562953086 4.758682454071576E-5 34-35 0.0 0.0 0.0 0.006543188374348417 4.758682454071576E-5 36-37 0.0 0.0 0.0 0.008113553584192038 4.758682454071576E-5 38-39 0.0 0.0 0.0 0.009493571495872793 4.758682454071576E-5 40-41 0.0 0.0 0.0 0.01168256542474572 4.758682454071576E-5 42-43 0.0 0.0 0.0 0.013990526414970434 4.758682454071576E-5 44-45 0.0 0.0 0.0 0.016679182001520874 4.758682454071576E-5 46-47 0.0 0.0 0.0 0.021390277631051738 4.758682454071576E-5 48-49 0.0 0.0 0.0 0.026101373260582594 4.758682454071576E-5 50-51 0.0 0.0 0.0 0.03354871130120461 4.758682454071576E-5 52-53 0.0 0.0 0.0 0.05115583638126944 4.758682454071576E-5 54-55 0.0 0.0 0.0 0.0716895511705883 4.758682454071576E-5 56-57 0.0 0.0 0.0 0.09652987358084192 4.758682454071576E-5 58-59 0.0 0.0 0.0 0.12689026763781858 7.138023681107364E-5 60-61 0.0 0.0 0.0 0.16962323607538132 9.517364908143153E-5 62-63 0.0 0.0 0.0 0.20095916003544267 9.517364908143153E-5 64-65 0.0 0.0 0.0 0.23165266186420433 9.517364908143153E-5 66-67 0.0 0.0 0.0 0.29301587210945734 9.517364908143153E-5 68-69 0.0 0.0 0.0 0.37010652786541687 9.517364908143153E-5 70-71 0.0 0.0 0.0 0.435871519380686 9.517364908143153E-5 72-73 0.0 0.0 0.0 0.4911436160847274 9.517364908143153E-5 74-75 0.0 0.0 0.0 0.5981187976522564 9.517364908143153E-5 76-77 0.0 0.0 0.0 0.6789212257223918 9.517364908143153E-5 78-79 0.0 0.0 0.0 0.7488024775604329 9.517364908143153E-5 80-81 0.0 0.0 0.0 0.8408116028099069 9.517364908143153E-5 82-83 0.0 0.0 0.0 0.989877330683699 9.517364908143153E-5 84-85 0.0 0.0 0.0 1.142987938643452 9.517364908143153E-5 86-87 0.0 0.0 0.0 1.3380939192603867 9.517364908143153E-5 88-89 0.0 0.0 0.0 1.5820715686806364 9.517364908143153E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAAG 505 0.0 26.346607 1 GTACATG 10110 0.0 26.320547 1 GTACCGT 200 7.166818E-10 26.133648 6 TACATGG 10725 0.0 24.1904 2 ACATGGG 10895 0.0 23.027903 3 GAGTACT 6080 0.0 22.30676 12-13 GTACTTT 6350 0.0 21.470243 14-15 GTATCAA 15855 0.0 20.829391 1 AGTACTT 6435 0.0 20.116478 12-13 GTATTAG 615 0.0 19.316256 1 GATGTGC 1455 0.0 19.263876 8 AGAGTAC 12295 0.0 19.048586 10-11 TATAACG 125 0.0060204193 19.006742 2 TAAGGTG 725 0.0 19.006289 5 GTCTTAG 680 0.0 18.867435 1 ACTTTTT 7380 0.0 18.859705 16-17 CATGGGG 5440 0.0 18.692291 4 TACTTTT 6945 0.0 18.399618 14-15 TCAACGC 18120 0.0 18.251507 4 ATCAACG 18245 0.0 18.10042 3 >>END_MODULE