FastQCFastQC Report
Thu 26 May 2016
SRR936466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101422
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72250.3438148073066714No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66860.31816550887922557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62220.29608522229233347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39190.18649276537506507No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31650.15061229967136538No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25870.12310711508683168No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG24430.1162546123529686No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG24240.11535046268669501No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG23320.11097247482894916No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22900.1089738281982391No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC21750.10350134337605678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95300.025.3371321
TACATGG102150.023.671792
ACATGGG101700.022.7439943
GCGCGAC2005.40329E-721.3697229
GATGTGC14200.021.0687398
GAGTACT79550.021.01303712-13
AGTACTT82700.020.87301812-13
GTATCAA165850.020.319081
GTACTTT81750.020.2151914-15
AGAGTAC136750.019.58565510-11
CATGGGG52150.019.3973624
TACTTTT87850.019.21693814-15
ATGGGAG23400.019.0764855
TAAGGTG6750.018.9953085
GTATAGT3809.094947E-1218.9491631
ATGGGGA30400.018.901585
CTCCTAA12700.018.8994271
GTAAGGT7050.018.8619354
GGTATCA131700.018.334311
ACTTTTT94600.017.84681116-17
Produced by FastQC (version 0.11.5)