##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936466_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2101422 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.401655164931174 31.0 31.0 34.0 30.0 34.0 2 31.61855591118776 31.0 31.0 34.0 30.0 34.0 3 31.89780015627513 31.0 31.0 34.0 30.0 34.0 4 35.7237789458757 37.0 35.0 37.0 33.0 37.0 5 35.51660970523769 37.0 35.0 37.0 33.0 37.0 6 35.61253379854213 37.0 35.0 37.0 33.0 37.0 7 35.53926579240153 37.0 35.0 37.0 33.0 37.0 8 35.512245517559066 37.0 35.0 37.0 33.0 37.0 9 37.129524198376146 39.0 37.0 39.0 33.0 39.0 10-11 37.002941579558986 39.0 37.0 39.0 33.0 39.0 12-13 36.8612234953284 39.0 37.0 39.0 32.5 39.0 14-15 37.96691835338166 40.0 37.0 41.0 33.0 41.0 16-17 37.87151414613533 40.0 37.0 41.0 33.0 41.0 18-19 37.9107290206346 39.5 37.0 41.0 33.0 41.0 20-21 37.96896672824402 40.0 37.0 41.0 33.0 41.0 22-23 37.88255571703351 40.0 37.0 41.0 33.0 41.0 24-25 37.75771382425805 39.5 37.0 41.0 32.0 41.0 26-27 37.54071742848414 39.0 36.5 41.0 32.0 41.0 28-29 37.44637083841323 39.0 36.5 41.0 32.0 41.0 30-31 37.4540304136913 39.0 36.0 41.0 32.0 41.0 32-33 37.293180284588246 39.0 36.0 41.0 31.5 41.0 34-35 37.11162631779814 39.0 36.0 41.0 31.0 41.0 36-37 36.87352540327454 39.0 35.5 41.0 30.5 41.0 38-39 36.71517762733996 39.0 35.0 41.0 30.0 41.0 40-41 36.47363356812673 39.0 35.0 40.0 30.0 41.0 42-43 36.22058682168551 38.5 35.0 40.0 30.0 41.0 44-45 35.93008567531891 38.0 35.0 40.0 29.5 41.0 46-47 35.532427565715025 38.0 34.5 40.0 27.5 41.0 48-49 35.06769796832812 38.0 34.0 40.0 26.0 41.0 50-51 34.83771489020292 38.0 34.0 40.0 26.0 41.0 52-53 34.529926402217164 38.0 33.5 40.0 25.5 41.0 54-55 34.206832087986136 37.0 33.0 40.0 24.5 41.0 56-57 33.88731463742171 37.0 33.0 40.0 24.0 41.0 58-59 33.48786250453264 36.5 32.5 39.0 23.0 41.0 60-61 33.064721174518965 36.0 32.0 39.0 22.0 40.0 62-63 32.74825261180287 35.5 31.5 39.0 22.0 40.0 64-65 32.28310210895289 35.0 31.0 38.0 20.5 40.0 66-67 31.842943254615207 35.0 31.0 38.0 20.0 40.0 68-69 31.379959617820695 34.5 30.0 37.0 18.5 39.5 70-71 30.88484702263515 34.0 30.0 36.5 18.0 39.0 72-73 30.466372056635933 34.0 29.0 36.0 15.5 39.0 74-75 30.098573251826622 34.0 29.0 35.5 12.5 38.0 76-77 28.7142075699217 32.5 27.0 34.5 10.0 36.0 78-79 29.532593881666795 33.5 29.0 35.0 9.0 37.0 80-81 29.394669419088597 34.0 29.0 35.0 7.0 36.0 82-83 29.076557207452858 34.0 29.0 35.0 4.5 36.0 84-85 28.632389401081745 33.0 28.5 35.0 2.0 35.5 86-87 28.241822680070925 33.0 27.0 35.0 2.0 35.0 88-89 27.820143693175382 33.0 27.0 35.0 2.0 35.0 90-91 27.351566938958477 33.0 26.0 35.0 2.0 35.0 92-93 26.861112142158973 32.0 25.0 34.0 2.0 35.0 94-95 26.268523409386596 32.0 24.5 34.0 2.0 35.0 96-97 25.5204087993749 32.0 23.5 34.0 2.0 35.0 98-99 24.57131194971786 31.5 17.0 34.0 2.0 35.0 100-101 21.685662851155072 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.46750359719024104 1101 2 0.4366795427101984 1101 3 0.23522847114269396 1101 4 0.20302994103089844 1101 5 0.27429956081804363 1101 6 0.3067423620767826 1101 7 0.308874184480338 1101 8 0.25505176515665795 1101 9 0.333171589662129 1101 10-11 0.40230455481215444 1101 12-13 0.321098501857378 1101 14-15 0.46454403697320856 1101 16-17 0.35600970453125313 1101 18-19 0.5207522636418744 1101 20-21 0.21300754066467675 1101 22-23 0.36920517055485647 1101 24-25 0.24655135223370195 1101 26-27 0.29907986355690497 1101 28-29 0.2340076148808521 1101 30-31 0.05710606900954218 1101 32-33 0.2517893302742138 1101 34-35 0.26000414231758384 1101 36-37 0.13378555864186126 1101 38-39 0.2421185380315407 1101 40-41 0.26381614593977787 1101 42-43 0.42285392184648174 1101 44-45 0.3589804602097928 1101 46-47 0.30702516842257666 1101 48-49 0.2553010109388296 1101 50-51 0.051279800461777825 1101 52-53 0.07817913007396271 1101 54-55 0.015848946793937557 1101 56-57 0.08527940278007407 1101 58-59 0.13322044200018723 1101 60-61 0.09206139108852796 1101 62-63 -0.04885857368867619 1101 64-65 0.07722965176066765 1101 66-67 -0.252619064517166 1101 68-69 -0.34024020051559845 1101 70-71 -0.2985628048811222 1101 72-73 -0.17768029784821238 1101 74-75 -0.053324465520876885 1101 76-77 0.27738084064835533 1101 78-79 0.2750328854967954 1101 80-81 -0.012987272252303228 1101 82-83 0.10101702202875984 1101 84-85 0.048572869323393775 1101 86-87 -0.1272368533698831 1101 88-89 -0.06749297090341955 1101 90-91 0.1300248364713319 1101 92-93 0.1073930703695467 1101 94-95 -0.23736836928738825 1101 96-97 -0.09450951445449007 1101 98-99 -0.06184310572260543 1101 100-101 -0.014586955636268328 1102 1 0.4390094687076278 1102 2 0.5264144106513804 1102 3 0.1865974452494008 1102 4 0.14538070562434768 1102 5 0.1571594478800975 1102 6 0.15210963583457016 1102 7 0.19806207464681336 1102 8 0.04142610921002188 1102 9 0.06461347266974826 1102 10-11 0.1799873543980226 1102 12-13 0.2329983680515113 1102 14-15 0.32256570676094043 1102 16-17 0.3781563064901974 1102 18-19 0.26204340975112217 1102 20-21 0.2938244327295578 1102 22-23 0.15928554263499706 1102 24-25 0.25082114415028656 1102 26-27 -0.0746976081079751 1102 28-29 0.10974970608460666 1102 30-31 0.16356868307058647 1102 32-33 0.28058013422043615 1102 34-35 0.39241036147259933 1102 36-37 0.3154077011781169 1102 38-39 0.13088335369021564 1102 40-41 0.3111071004377308 1102 42-43 0.2522027332929184 1102 44-45 0.13898372887297228 1102 46-47 0.14153057781862088 1102 48-49 0.10722855610837456 1102 50-51 0.13846495010374582 1102 52-53 -0.027798015046641922 1102 54-55 0.27155481702317275 1102 56-57 0.10339955110975296 1102 58-59 -0.1794461108804981 1102 60-61 0.08050050780878593 1102 62-63 0.24657765598080061 1102 64-65 0.08363997526171119 1102 66-67 0.03768230461795952 1102 68-69 0.06963607333718969 1102 70-71 -0.12892683412841066 1102 72-73 0.016113388318256483 1102 74-75 -0.3669255259847084 1102 76-77 -0.04447902870172982 1102 78-79 0.04605779657466513 1102 80-81 0.1323994041613119 1102 82-83 0.15045474755444133 1102 84-85 0.2387255384364586 1102 86-87 0.1294294885773688 1102 88-89 -0.012640414985927606 1102 90-91 -0.12192992072174391 1102 92-93 0.2558813539027298 1102 94-95 0.017137313839064916 1102 96-97 -0.13383793298507385 1102 98-99 -0.5548875353891844 1102 100-101 -0.5930308465369833 1103 1 0.5486302137889112 1103 2 0.5352740478300042 1103 3 0.333238272122788 1103 4 0.1829125749635807 1103 5 0.12909212668880343 1103 6 0.10056308273152581 1103 7 0.13703140965166938 1103 8 0.31246065565200354 1103 9 0.1032084358664278 1103 10-11 0.16494420460390558 1103 12-13 0.27630780231498164 1103 14-15 0.2196788024182581 1103 16-17 0.2566554777699963 1103 18-19 0.33098623979540065 1103 20-21 0.2532594004963258 1103 22-23 0.16931333097718948 1103 24-25 0.18802817708154151 1103 26-27 0.329793171108129 1103 28-29 0.3126601651752523 1103 30-31 0.28467843254510683 1103 32-33 0.09940460361256243 1103 34-35 0.1742292262433054 1103 36-37 0.15784052319342834 1103 38-39 0.17718291061053293 1103 40-41 0.3034525898991376 1103 42-43 0.2844066125486222 1103 44-45 0.37346576700819156 1103 46-47 0.29640343695809435 1103 48-49 0.46484479996340156 1103 50-51 0.270269415958758 1103 52-53 0.26138268665009434 1103 54-55 0.3814136528708474 1103 56-57 0.3411244754763203 1103 58-59 0.40228573435619097 1103 60-61 0.36317098236261103 1103 62-63 0.11331197407476168 1103 64-65 0.1211411295226128 1103 66-67 0.21114443781732106 1103 68-69 0.10229617910661659 1103 70-71 0.13233914358144006 1103 72-73 0.0359696104497047 1103 74-75 0.18147224249637972 1103 76-77 0.0977959530257344 1103 78-79 0.1454474860119639 1103 80-81 0.1432874103340609 1103 82-83 0.2957969330907311 1103 84-85 0.4293255115167476 1103 86-87 0.4093829503427706 1103 88-89 0.34213145610621254 1103 90-91 0.24073807314932694 1103 92-93 0.22960489785150884 1103 94-95 0.2670304957959715 1103 96-97 0.040378186914072245 1103 98-99 0.056903611468801074 1103 100-101 0.3054157139220308 1104 1 0.30216824038057055 1104 2 0.424937725888487 1104 3 0.17137210721711327 1104 4 0.014729000645928636 1104 5 -0.027137964610574272 1104 6 0.07668650803284294 1104 7 0.11689334940160734 1104 8 0.09104133458689745 1104 9 0.0419932534177363 1104 10-11 0.04780725097842975 1104 12-13 -0.011909494990938185 1104 14-15 0.10957079540241921 1104 16-17 0.009834031818513722 1104 18-19 0.11647173571935099 1104 20-21 0.08848772871822064 1104 22-23 0.10474256493692735 1104 24-25 0.11314857426594216 1104 26-27 0.17724869433489943 1104 28-29 0.007979595231297765 1104 30-31 0.009034696522753904 1104 32-33 0.1959452525967933 1104 34-35 0.10973073460338867 1104 36-37 0.17552229853382073 1104 38-39 0.14767738527536522 1104 40-41 0.26214591812672694 1104 42-43 0.19336565604204736 1104 44-45 0.23951119673980514 1104 46-47 0.30385482316399504 1104 48-49 0.3453763558593437 1104 50-51 0.2685468919125569 1104 52-53 0.1706778393456716 1104 54-55 0.28343235949908063 1104 56-57 0.3701205660105913 1104 58-59 0.23661305438344016 1104 60-61 0.0566305575145023 1104 62-63 -0.08047174368999777 1104 64-65 -0.043869779068216985 1104 66-67 -5.651357200271434E-4 1104 68-69 0.05533548384397946 1104 70-71 -0.02062444873547875 1104 72-73 -0.19772752619241274 1104 74-75 0.0022034276939741915 1104 76-77 0.21522675289915583 1104 78-79 0.2697896560066724 1104 80-81 0.21374659305780597 1104 82-83 0.05619855841947796 1104 84-85 0.28894849177491366 1104 86-87 0.21265773096479634 1104 88-89 0.16360855870243185 1104 90-91 0.0882303509003215 1104 92-93 0.029020263483992892 1104 94-95 0.09048199065090756 1104 96-97 -0.10226355733598425 1104 98-99 0.06708081576202929 1104 100-101 0.11724594107068498 1105 1 0.2781078633584855 1105 2 0.3645000759596968 1105 3 0.061429273041063936 1105 4 0.07124642775134049 1105 5 -0.08807064749465354 1105 6 -0.06523383075781197 1105 7 0.0514721284852655 1105 8 -0.07625241789322956 1105 9 0.04316388075726252 1105 10-11 0.06941767760768869 1105 12-13 -0.11722487593494435 1105 14-15 -0.044503159612474974 1105 16-17 -0.017801121527426744 1105 18-19 0.022991722037481566 1105 20-21 0.07376706159284652 1105 22-23 0.010966116984420182 1105 24-25 -0.17699806012380037 1105 26-27 -0.0835714398509495 1105 28-29 0.03484547378635483 1105 30-31 -0.009541504838750825 1105 32-33 -0.050050901595867 1105 34-35 -0.13806820080189652 1105 36-37 0.0028978287205347897 1105 38-39 0.021492049545372538 1105 40-41 -0.11000544674580937 1105 42-43 0.0636028053472728 1105 44-45 -0.08774411655410574 1105 46-47 0.10628463982425984 1105 48-49 -0.040586352917166835 1105 50-51 -0.22581764612498745 1105 52-53 -0.1639073991531248 1105 54-55 0.027015015553921273 1105 56-57 -0.023532435341536484 1105 58-59 0.01515899470712867 1105 60-61 -0.029263420818502084 1105 62-63 -0.07168382272075746 1105 64-65 0.08161597556050992 1105 66-67 -0.02909743005706744 1105 68-69 -0.19032353420782044 1105 70-71 -0.1698402436297819 1105 72-73 -0.26053164518390304 1105 74-75 -0.3555932091416487 1105 76-77 -0.06482562880241716 1105 78-79 -0.06421706820671247 1105 80-81 -0.17165828289403962 1105 82-83 -0.2558837398177616 1105 84-85 -0.053102848487618814 1105 86-87 -0.17585248048283475 1105 88-89 -0.12259226073670604 1105 90-91 -0.4449162700795135 1105 92-93 -0.3435164942711282 1105 94-95 -0.06483209652135358 1105 96-97 -0.10675870588081082 1105 98-99 -0.19036002440011757 1105 100-101 -0.08013848686594471 1106 1 0.3753826642892264 1106 2 0.34870265898144126 1106 3 0.115405865528313 1106 4 0.05745717479904755 1106 5 -0.07455960077853518 1106 6 -0.049287095127063196 1106 7 6.636882770720831E-4 1106 8 0.10989466823537697 1106 9 0.06408300389397681 1106 10-11 -0.06824199501403427 1106 12-13 -0.0489487311363348 1106 14-15 0.01531041738648753 1106 16-17 0.05117178000131162 1106 18-19 -0.04241366664737711 1106 20-21 -0.06428557495065945 1106 22-23 -0.08097970745885164 1106 24-25 -0.12913388968821238 1106 26-27 -0.062450297360861384 1106 28-29 -0.016810091999580834 1106 30-31 -0.09666846120906314 1106 32-33 -0.10049120067616713 1106 34-35 -0.3314176205786339 1106 36-37 -0.19701846118066868 1106 38-39 0.012800061846782285 1106 40-41 0.035752185022182914 1106 42-43 -0.20480377368602376 1106 44-45 -0.06865376502133813 1106 46-47 -0.13598783349218735 1106 48-49 -0.3359457125043619 1106 50-51 -0.1608431238587542 1106 52-53 -0.22926581547415026 1106 54-55 -0.27847357149828156 1106 56-57 -0.3422594002661654 1106 58-59 -0.3865291844110956 1106 60-61 -0.18423384159540745 1106 62-63 -0.4896480213779597 1106 64-65 -0.2909292888041577 1106 66-67 -0.33648410159154807 1106 68-69 -0.3488295800856882 1106 70-71 -0.3230473867897068 1106 72-73 -0.037956536183195055 1106 74-75 0.08677742877742034 1106 76-77 -0.07367915560588045 1106 78-79 -0.0535242678480401 1106 80-81 -0.20928295537636643 1106 82-83 -0.07208613113115803 1106 84-85 0.07215736122297756 1106 86-87 -0.01216935452166723 1106 88-89 -0.02442832642316617 1106 90-91 -0.17045073347372508 1106 92-93 0.24109772705119425 1106 94-95 0.21140196867705185 1106 96-97 0.057266879163801576 1106 98-99 0.0330693705996552 1106 100-101 0.052093395454154034 1107 1 0.6344912478435667 1107 2 0.5992680209061838 1107 3 0.40175583262172765 1107 4 0.2901323915760017 1107 5 0.11627020382130127 1107 6 0.31469765838240704 1107 7 0.2577038579287958 1107 8 0.22514833508265752 1107 9 0.39736970631807367 1107 10-11 0.34029007607509243 1107 12-13 0.44238143977351996 1107 14-15 0.3973451934468528 1107 16-17 0.4491394520636902 1107 18-19 0.506422067042287 1107 20-21 0.40795991225983386 1107 22-23 0.5452417990495704 1107 24-25 0.3696887177033048 1107 26-27 0.40249045034760655 1107 28-29 0.3989421243690501 1107 30-31 0.5490664911252097 1107 32-33 0.6314459457029571 1107 34-35 0.6143597537711258 1107 36-37 0.7071373066464588 1107 38-39 0.7141502083480802 1107 40-41 0.8247279794925078 1107 42-43 0.6928258621341499 1107 44-45 0.6239929417993935 1107 46-47 0.793233372791903 1107 48-49 0.8296677466294753 1107 50-51 0.8995211558958189 1107 52-53 0.5795964342461701 1107 54-55 0.8448284691641703 1107 56-57 0.9837971645407251 1107 58-59 0.8854145569109022 1107 60-61 0.7860673410885894 1107 62-63 0.682367006189537 1107 64-65 0.6978461365855608 1107 66-67 0.8263327520302433 1107 68-69 0.8528095493636805 1107 70-71 0.8453311111530049 1107 72-73 0.7567386225746127 1107 74-75 0.986353299462035 1107 76-77 1.052531790818385 1107 78-79 1.099569814099123 1107 80-81 1.0140635057691085 1107 82-83 0.8517798634448184 1107 84-85 0.8909749315679001 1107 86-87 1.265627057956582 1107 88-89 1.270566261817578 1107 90-91 1.14235474250901 1107 92-93 1.4355202312491109 1107 94-95 1.4239245706636154 1107 96-97 1.281633467148378 1107 98-99 1.5171711071316878 1107 100-101 1.745658261849787 1108 1 0.3197694097648309 1108 2 0.33048607438766453 1108 3 0.06861490649894719 1108 4 0.06123295999882572 1108 5 0.024650441546896218 1108 6 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-1.7562670261785343 2316 76-77 -1.8062772020942894 2316 78-79 -1.5637720437038816 2316 80-81 -1.5394629814534433 2316 82-83 -1.3103355197860616 2316 84-85 -1.6776798752353557 2316 86-87 -1.3979073846377226 2316 88-89 -1.6727388583152205 2316 90-91 -1.7929062985904842 2316 92-93 -1.8835597276935587 2316 94-95 -2.0418517748844067 2316 96-97 -1.982394625033141 2316 98-99 -2.213577613169374 2316 100-101 -2.6015610606014334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 48.0 5 111.0 6 240.0 7 557.0 8 1204.0 9 2092.0 10 3085.0 11 4666.0 12 6429.0 13 8641.0 14 10943.0 15 13989.0 16 17653.0 17 21556.0 18 19062.0 19 14500.0 20 14180.0 21 14937.0 22 15995.0 23 18126.0 24 21411.0 25 26234.0 26 32166.0 27 39493.0 28 48803.0 29 59956.0 30 72406.0 31 88251.0 32 109081.0 33 136174.0 34 176309.0 35 234434.0 36 317437.0 37 366795.0 38 177215.0 39 7225.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43564211239942 18.49861646348353 14.587066638138488 23.47867478597856 2 16.557125044022882 20.23510598806385 37.4809868740422 25.726782093871066 3 20.11958078198636 23.433988105690602 28.87660784232545 27.56982326999759 4 13.971813745320455 16.254546285253046 34.75027675109915 35.02336321832735 5 14.93840837299695 36.609019987418044 32.51246061000599 15.940111029579018 6 34.193750707854015 34.50277954642142 16.03661710974759 15.26685263597697 7 30.733284414077705 31.69382446743205 19.055144564014274 18.51774655447597 8 28.334765696752008 32.74673054721993 18.525170099104322 20.39333365692374 9 27.08502074384704 16.281802235329167 19.29867808537956 37.33449893544423 10-11 26.70369564917387 25.23747071618817 25.60876779810956 22.4500658365284 12-13 27.514879837062363 22.45403400323829 26.495234764153242 23.5358513955461 14-15 23.855690982435217 24.0870820268761 24.977051242944658 27.08017574774403 16-17 23.80238238678381 27.459120538378297 25.365942680718106 23.372554394119792 18-19 23.671352065410943 25.4469354560864 27.824777698149155 23.056934780353494 20-21 24.342617604321646 24.490160588915646 27.72987237448556 23.437349432277152 22-23 23.809170302351966 24.490004720680385 27.971173393636217 23.729651583331428 24-25 24.22300232889919 24.823476674366216 27.820471090528226 23.133049906206367 26-27 23.834509164391818 25.12711038694522 27.88419648319007 23.154183965472896 28-29 23.2336008664609 25.432778375785542 28.04229707312477 23.29132368462879 30-31 23.348325573106383 24.849928893336674 27.977694135942805 23.824051397614138 32-33 23.48745413364126 24.936047049764927 27.96148379886354 23.615015017730272 34-35 24.427177230944793 24.803305013506385 27.393561524183603 23.375956231365222 36-37 24.38618557054262 24.469321889359694 27.627740595650074 23.516751944447616 38-39 23.763091454653132 24.70350384752014 27.899487893534776 23.633916804291957 40-41 23.721629338001428 24.845546198372336 27.880847443068323 23.55197702055791 42-43 23.955378463303 24.94910712904719 27.10294339856108 23.992571009088735 44-45 23.79284991969544 24.996752126583786 27.084171078460535 24.126226875260244 46-47 24.210505026470763 25.457950151686397 26.62606626613527 23.705478555707575 48-49 24.666525004586568 25.353641615718686 26.315500171446015 23.66433320824873 50-51 24.041840448669856 25.508045425657944 26.459429768990983 23.99068435668121 52-53 24.575720298755343 25.636211355843884 25.953002424591396 23.83506592080937 54-55 24.029931047512896 25.240389019857183 26.291083387131202 24.43859654549872 56-57 24.233516478940633 25.70075145139979 26.108349769325052 23.957382300334526 58-59 24.897996379549596 25.05539174910393 25.997027690164064 24.049584181182414 60-61 24.38241024425669 25.23984992247708 26.903332866958486 23.474406966307747 62-63 23.974149036358956 25.438419913265403 26.66731862756995 23.920112422805687 64-65 23.877941297557292 25.599325296479748 26.315602852069254 24.207130553893705 66-67 24.039039692844856 25.727645842657704 26.017245592532607 24.216068871964836 68-69 23.82595263718544 25.33153232593348 26.539003572114332 24.303511464766743 70-71 24.07664908310908 25.544650101480208 26.275450355597112 24.103250459813598 72-73 24.16942531519736 25.363712134857288 26.41692777960062 24.04993477034474 74-75 23.85490012355922 26.10732782547433 25.810623903866976 24.227148147099477 76-77 24.095892467648284 25.809277691895694 25.96852900846128 24.126300831994737 78-79 24.19402660458201 25.641259327332683 26.14004169964359 24.024672368441717 80-81 24.097233063125152 26.17878954602741 25.929253113613086 23.79472427723436 82-83 24.17434464063179 25.90657361800633 25.874853565436233 24.044228175925646 84-85 23.94727048445403 25.80756615579664 25.818749964306992 24.42641339544234 86-87 24.081116723197344 25.669861161544798 25.847805488147596 24.401216627110266 88-89 23.87969580514698 25.901735247280293 25.490134944637212 24.728434002935515 90-91 24.019025932423954 25.7875589860071 25.88646794121819 24.30694714035076 92-93 24.131575553581175 25.621156363750575 26.01390145348519 24.23336662918306 94-95 23.904852213495968 25.427035953470718 26.010686457990033 24.657425375043278 96-97 23.878549862745622 25.44912259995203 26.196145265009463 24.47618227229289 98-99 23.840445798791844 25.65351413014458 25.567659123260473 24.938380947803108 100-101 23.389132679370423 25.583699138035858 25.971408100030956 25.055760082562756 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 433.0 1 337.5 2 334.5 3 405.5 4 324.5 5 317.0 6 524.0 7 1081.0 8 1775.0 9 2073.5 10 1846.0 11 1612.0 12 1705.5 13 1909.0 14 2087.0 15 2284.0 16 2494.0 17 2704.5 18 2818.0 19 2912.5 20 3104.0 21 3365.5 22 3835.0 23 4372.5 24 5055.5 25 6010.5 26 7066.0 27 8370.5 28 9933.0 29 11941.5 30 14342.0 31 17259.0 32 20955.5 33 23422.5 34 25172.5 35 27254.0 36 30667.5 37 34740.0 38 37805.0 39 39270.5 40 41008.0 41 47848.0 42 55204.5 43 60073.5 44 62965.5 45 66560.5 46 70041.0 47 76864.0 48 93593.5 49 96473.0 50 86277.0 51 82251.5 52 81874.5 53 84992.0 54 86973.0 55 83202.0 56 77093.5 57 71878.5 58 68311.0 59 62507.0 60 53805.0 61 45244.5 62 38038.5 63 30628.0 64 23909.5 65 19882.5 66 15827.5 67 12932.5 68 9776.5 69 6468.0 70 4963.0 71 4044.5 72 3571.5 73 3207.0 74 2660.5 75 2222.5 76 1554.0 77 966.5 78 649.0 79 472.0 80 332.5 81 219.5 82 147.0 83 91.0 84 51.5 85 25.5 86 16.0 87 10.5 88 3.5 89 0.5 90 1.5 91 2.5 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0282085178512455 2 0.011516011538853215 3 0.003854532787797977 4 0.012182227082423235 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003616598665094398 10-11 2.379341227035788E-4 12-13 2.141407104332209E-4 14-15 4.758682454071576E-5 16-17 0.0 18-19 0.0 20-21 2.379341227035788E-5 22-23 0.001427604736221473 24-25 0.0 26-27 2.379341227035788E-5 28-29 0.0 30-31 7.138023681107365E-5 32-33 0.0034500447792018933 34-35 4.04488008596084E-4 36-37 0.002569688525198651 38-39 1.665538858925052E-4 40-41 0.0031169370074168824 42-43 0.009255637373169217 44-45 0.002236580753413641 46-47 0.002236580753413641 48-49 0.008446661355977048 50-51 4.996616576775155E-4 52-53 0.0017607125080064832 54-55 0.009160463724087785 56-57 0.004211433971853345 58-59 0.0012372574380586099 60-61 0.005139377050397302 62-63 0.0032834908933093874 64-65 0.016702975413791232 66-67 0.0034976316037426086 68-69 2.617275349739367E-4 70-71 0.0012848442625993256 72-73 2.379341227035788E-5 74-75 2.379341227035788E-5 76-77 0.0012610508503289677 78-79 0.009755299030846732 80-81 0.01551330480027334 82-83 0.010826002583012835 84-85 0.00808976017192168 86-87 0.0096601253817653 88-89 0.028433127663077668 90-91 0.023793412270357882 92-93 0.00280762264790223 94-95 0.007828032636947743 96-97 0.010183580451713173 98-99 0.009755299030846732 100-101 0.011063936705716414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2101422.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.546252968869624 #Duplication Level Percentage of deduplicated Percentage of total 1 72.66010223528176 30.914150904462268 2 14.329147871297637 12.19303100321138 3 5.0482733753884155 6.443553482558545 4 2.4589560200548566 4.184774594743151 5 1.3406677136705822 2.852019384651233 6 0.8350926540116836 2.131803798601548 7 0.5490423433797518 1.635178610243913 8 0.38705325515325895 1.3174132564939995 9 0.2968215193655714 1.136577910457865 >10 1.7589584676868701 14.111702171305089 >50 0.182216943658127 5.417832841867613 >100 0.1402526933773223 11.479064894865862 >500 0.009356616277004133 2.6056121695030012 >1k 0.003720100447483571 2.6116554477373164 >5k 3.381909497712337E-4 0.9656295292972782 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7225 0.3438148073066714 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6686 0.31816550887922557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6222 0.29608522229233347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3919 0.18649276537506507 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3165 0.15061229967136538 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2587 0.12310711508683168 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2443 0.1162546123529686 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2424 0.11535046268669501 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2332 0.11097247482894916 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2290 0.1089738281982391 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2175 0.10350134337605678 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.758682454071576E-5 0.0 20-21 0.0 0.0 0.0 9.517364908143153E-5 0.0 22-23 0.0 0.0 0.0 1.427604736221473E-4 0.0 24-25 0.0 0.0 0.0 1.427604736221473E-4 0.0 26-27 0.0 0.0 0.0 3.806945963257261E-4 0.0 28-29 0.0 0.0 0.0 0.001213464025788252 2.379341227035788E-5 30-31 0.0 0.0 0.0 0.00280762264790223 4.758682454071576E-5 32-33 0.0 0.0 0.0 0.004925236339964081 4.758682454071576E-5 34-35 0.0 0.0 0.0 0.006828709321592711 4.758682454071576E-5 36-37 0.0 0.0 0.0 0.008446661355977048 4.758682454071576E-5 38-39 0.0 0.0 0.0 0.009731505618576374 4.758682454071576E-5 40-41 0.0 0.0 0.0 0.011849119310638225 4.758682454071576E-5 42-43 0.0 0.0 0.0 0.014347427599025802 4.758682454071576E-5 44-45 0.0 0.0 0.0 0.017107463422387316 4.758682454071576E-5 46-47 0.0 0.0 0.0 0.0215806249292146 4.758682454071576E-5 48-49 0.0 0.0 0.0 0.02636310079555653 4.758682454071576E-5 50-51 0.0 0.0 0.0 0.03376285201163784 4.758682454071576E-5 52-53 0.0 0.0 0.0 0.05115583638126944 4.758682454071576E-5 54-55 0.0 0.0 0.0 0.07104712903928864 4.758682454071576E-5 56-57 0.0 0.0 0.0 0.09553055026548689 4.758682454071576E-5 58-59 0.0 0.0 0.0 0.1259147377347339 4.758682454071576E-5 60-61 0.0 0.0 0.0 0.16826701157597096 4.758682454071576E-5 62-63 0.0 0.0 0.0 0.19948396847468047 4.758682454071576E-5 64-65 0.0 0.0 0.0 0.22977298229484605 4.758682454071576E-5 66-67 0.0 0.0 0.0 0.2904223901719883 4.758682454071576E-5 68-69 0.0 0.0 0.0 0.36670406991075566 4.758682454071576E-5 70-71 0.0 0.0 0.0 0.4318266392947252 4.758682454071576E-5 72-73 0.0 0.0 0.0 0.48669424799017047 4.758682454071576E-5 74-75 0.0 0.0 0.0 0.5935742559086181 4.758682454071576E-5 76-77 0.0 0.0 0.0 0.6743766839787535 4.758682454071576E-5 78-79 0.0 0.0 0.0 0.7437106873345762 4.758682454071576E-5 80-81 0.0 0.0 0.0 0.8348870431545877 4.758682454071576E-5 82-83 0.0 0.0 0.0 0.9834055225461616 4.758682454071576E-5 84-85 0.0 0.0 0.0 1.1358261215500742 4.758682454071576E-5 86-87 0.0 0.0 0.0 1.3288858687117582 4.758682454071576E-5 88-89 0.0 0.0 0.0 1.5709124583258385 4.758682454071576E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9530 0.0 25.337132 1 TACATGG 10215 0.0 23.67179 2 ACATGGG 10170 0.0 22.743994 3 GCGCGAC 200 5.40329E-7 21.369722 9 GATGTGC 1420 0.0 21.068739 8 GAGTACT 7955 0.0 21.013037 12-13 AGTACTT 8270 0.0 20.873018 12-13 GTATCAA 16585 0.0 20.31908 1 GTACTTT 8175 0.0 20.21519 14-15 AGAGTAC 13675 0.0 19.585655 10-11 CATGGGG 5215 0.0 19.397362 4 TACTTTT 8785 0.0 19.216938 14-15 ATGGGAG 2340 0.0 19.076485 5 TAAGGTG 675 0.0 18.995308 5 GTATAGT 380 9.094947E-12 18.949163 1 ATGGGGA 3040 0.0 18.90158 5 CTCCTAA 1270 0.0 18.899427 1 GTAAGGT 705 0.0 18.861935 4 GGTATCA 13170 0.0 18.33431 1 ACTTTTT 9460 0.0 17.846811 16-17 >>END_MODULE