FastQCFastQC Report
Thu 26 May 2016
SRR936465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097899
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64340.3066877862089643No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59840.2852377545344175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50640.24138435644423303No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36290.17298258877095607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.14252354379309967No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27620.13165552774466263No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG24390.1162591716760435No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23880.11382816808626153No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG22660.10801282616560665No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG22420.10686882447629747No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG21440.10219748424495174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG102750.025.9017891
TACATGG107150.024.3952052
ACATGGG108050.023.7081643
TAAGGTG7450.023.607045
GTAAGGT6750.023.237854
GTGTAAG5600.022.9139161
GAGTACT62150.022.29268812-13
GTATCAA152250.021.8818631
GTACTTT64200.021.39576514-15
AGTACTT64950.021.25846912-13
ACTTGAC10350.021.1258228
GATGTGC13700.020.12358
ATTCGCG6250.019.75938680-81
AGAGTAC122500.019.55530710-11
TACTTTT70700.019.42868814-15
GTACTAG2451.7479579E-719.3980261
CATGGGA54100.019.3290794
CATGGGG58400.019.2081224
CGCGGAA6700.019.13982882-83
TCAACGC173950.018.9362744
Produced by FastQC (version 0.11.5)