##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936465_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2097899 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.281966863037734 31.0 31.0 34.0 28.0 34.0 2 31.308434295454642 31.0 31.0 34.0 28.0 34.0 3 31.36662870805506 31.0 31.0 34.0 28.0 34.0 4 35.00055484081931 37.0 35.0 37.0 32.0 37.0 5 34.95371369165055 37.0 35.0 37.0 32.0 37.0 6 35.09453505626343 37.0 35.0 37.0 32.0 37.0 7 34.94188042417676 37.0 35.0 37.0 32.0 37.0 8 34.882746500189 37.0 35.0 37.0 32.0 37.0 9 36.34190063487327 39.0 37.0 39.0 32.0 39.0 10-11 36.23414854575935 39.0 35.0 39.0 32.0 39.0 12-13 36.11332814401456 39.0 35.0 39.0 31.0 39.0 14-15 37.153901355594336 39.0 36.0 41.0 31.0 41.0 16-17 37.01924187008049 39.0 36.0 41.0 31.0 41.0 18-19 36.93399634586794 39.0 36.0 41.0 31.0 41.0 20-21 36.824465810794514 39.0 36.0 41.0 30.5 41.0 22-23 36.68236030428538 39.0 36.0 40.5 30.0 41.0 24-25 36.80862972907657 39.0 36.0 41.0 30.0 41.0 26-27 36.79311134616108 39.0 36.0 41.0 30.0 41.0 28-29 36.68312583208248 39.0 36.0 41.0 30.0 41.0 30-31 36.49942156414585 39.0 35.5 41.0 30.0 41.0 32-33 36.32907875927297 39.0 35.0 40.0 30.0 41.0 34-35 36.09716435347936 38.5 35.0 40.0 29.5 41.0 36-37 35.89509147008507 38.0 35.0 40.0 28.5 41.0 38-39 35.68469573606737 38.0 35.0 40.0 27.5 41.0 40-41 35.48587491580862 38.0 34.5 40.0 27.0 41.0 42-43 35.2341509290962 38.0 34.0 40.0 26.5 41.0 44-45 34.96624599182324 38.0 34.0 40.0 25.5 41.0 46-47 34.652867464067626 38.0 33.0 40.0 24.0 41.0 48-49 34.539525020031945 38.0 33.0 40.0 24.0 41.0 50-51 33.57968376933303 37.0 32.0 39.5 23.0 40.5 52-53 33.70749020806054 37.5 33.0 39.5 23.0 40.0 54-55 34.08578582667707 38.0 33.0 40.0 23.0 41.0 56-57 33.96042540656151 37.5 33.0 40.0 23.0 41.0 58-59 33.66708835840048 37.0 33.0 40.0 22.5 41.0 60-61 33.30851628224238 36.5 32.5 39.5 22.0 41.0 62-63 32.95252869656737 36.0 32.0 39.0 21.0 41.0 64-65 32.58662785958714 35.5 31.5 39.0 20.0 41.0 66-67 32.173710936513146 35.0 31.0 38.5 18.5 40.0 68-69 31.754808262933533 35.0 31.0 38.0 18.0 40.0 70-71 31.30211011111593 35.0 30.5 37.0 15.5 39.5 72-73 30.91964651301135 35.0 30.0 36.5 12.5 39.0 74-75 30.492426232149402 34.0 30.0 36.0 9.5 39.0 76-77 30.075645443369773 34.0 29.5 35.5 7.0 37.5 78-79 29.63736004450167 34.0 29.0 35.0 6.0 37.0 80-81 29.18095580387807 34.0 29.0 35.0 2.0 37.0 82-83 28.72436327964311 34.0 28.5 35.0 2.0 36.0 84-85 28.427058928957017 34.0 27.5 35.0 2.0 36.0 86-87 28.114968833103976 33.5 27.0 35.0 2.0 35.5 88-89 27.71619629924987 33.0 26.5 35.0 2.0 35.0 90-91 27.308803235999445 33.0 26.0 35.0 2.0 35.0 92-93 26.92549688998374 33.0 25.0 35.0 2.0 35.0 94-95 26.43279323742468 33.0 25.0 35.0 2.0 35.0 96-97 25.805069262152276 32.5 23.5 35.0 2.0 35.0 98-99 25.044573165819706 32.0 19.0 35.0 2.0 35.0 100-101 22.50630916931654 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4790098486036065 1101 2 0.461320737231933 1101 3 0.6170236255750687 1101 4 0.633065840861768 1101 5 0.5221434878968623 1101 6 0.37745004893662326 1101 7 0.49944344912747596 1101 8 0.5066640193950178 1101 9 0.8343334585757489 1101 10-11 0.7090165108108621 1101 12-13 0.6457876892264807 1101 14-15 0.8918114176033356 1101 16-17 0.9890330675555248 1101 18-19 1.0637146228448202 1101 20-21 1.0187039769349013 1101 22-23 0.8610108751852223 1101 24-25 0.9965126167071929 1101 26-27 0.8236859294940615 1101 28-29 0.8240359335815484 1101 30-31 0.8377384498639557 1101 32-33 0.8310627847286582 1101 34-35 0.8456688021961654 1101 36-37 0.7081157874877633 1101 38-39 0.6847815366380487 1101 40-41 0.7230960067348278 1101 42-43 0.6879302137918017 1101 44-45 0.44987032617033407 1101 46-47 0.6384701807030453 1101 48-49 0.7302451116431641 1101 50-51 0.672439344757116 1101 52-53 0.7142368250830202 1101 54-55 0.7558494587872886 1101 56-57 0.6862208223138495 1101 58-59 0.6670192853246135 1101 60-61 0.6353910081633884 1101 62-63 0.7497552910568714 1101 64-65 0.7301988838795808 1101 66-67 0.6015481041342738 1101 68-69 0.6158349091218724 1101 70-71 0.5499750246628281 1101 72-73 0.4387395277758479 1101 74-75 0.28876103882128845 1101 76-77 0.3643843660418078 1101 78-79 0.4193733161828206 1101 80-81 0.509394543917761 1101 82-83 0.6050927317806369 1101 84-85 0.6897630954401954 1101 86-87 0.42769469613362077 1101 88-89 0.46685670259655865 1101 90-91 0.4928093571660739 1101 92-93 0.3135983964371576 1101 94-95 0.4039629808906646 1101 96-97 0.33705576566088524 1101 98-99 0.30965742318261746 1101 100-101 0.8178859373881942 1102 1 0.3709696554661228 1102 2 0.4334221876418205 1102 3 0.30028426097483774 1102 4 0.30397885227841925 1102 5 0.42268339921895404 1102 6 0.3774887985530384 1102 7 0.3247067508139807 1102 8 0.3478994041356529 1102 9 0.5483685305012997 1102 10-11 0.5312550918077363 1102 12-13 0.4607188362464427 1102 14-15 0.5973341968980463 1102 16-17 0.464077825319535 1102 18-19 0.6057885845116644 1102 20-21 0.7182397447879012 1102 22-23 0.47573185446239563 1102 24-25 0.4277880192335175 1102 26-27 0.4385129809535542 1102 28-29 0.48884617397008157 1102 30-31 0.5817464479413488 1102 32-33 0.43070399850755337 1102 34-35 0.18378171153302247 1102 36-37 0.30864775318143955 1102 38-39 0.11650862661230832 1102 40-41 0.022467910679615954 1102 42-43 0.1750217967488652 1102 44-45 0.03501977052431471 1102 46-47 -0.0022664690755718198 1102 48-49 0.10651014962561334 1102 50-51 0.2152509190718277 1102 52-53 0.13066779753400226 1102 54-55 0.3397132182672422 1102 56-57 0.3800184178022974 1102 58-59 0.3420754722684194 1102 60-61 0.2531661617527803 1102 62-63 0.3035268421061659 1102 64-65 0.3307147038997513 1102 66-67 0.2891007488151658 1102 68-69 0.17991033544593193 1102 70-71 0.028966470000035827 1102 72-73 0.06019256793766914 1102 74-75 0.039819510035414396 1102 76-77 0.1534598315068969 1102 78-79 0.11816119686844218 1102 80-81 0.27036939424247564 1102 82-83 0.14646988531992733 1102 84-85 0.2408327414174387 1102 86-87 0.2518259414758397 1102 88-89 0.2566365108368416 1102 90-91 0.0803775212640403 1102 92-93 0.25509303176803755 1102 94-95 0.3618776683260805 1102 96-97 0.14966986175367225 1102 98-99 0.29521095949609943 1102 100-101 0.36270867550654273 1103 1 0.20224423851902174 1103 2 0.3424305451079661 1103 3 0.3156260927634662 1103 4 0.3540512893118475 1103 5 0.43960634196089643 1103 6 0.3523812814645382 1103 7 0.25441253373598016 1103 8 0.27688641255178226 1103 9 0.611113396232291 1103 10-11 0.5390306882844556 1103 12-13 0.4922938766907663 1103 14-15 0.2770510749969759 1103 16-17 0.5350303240691545 1103 18-19 0.420193385192114 1103 20-21 0.5631362142447145 1103 22-23 0.6627697162230533 1103 24-25 0.7121016387851 1103 26-27 0.47268400336156446 1103 28-29 0.07433354746576981 1103 30-31 0.21275241193662708 1103 32-33 0.4437196058517543 1103 34-35 0.31623207460580005 1103 36-37 0.3192908257774789 1103 38-39 0.36782075188477137 1103 40-41 0.20151956056137266 1103 42-43 0.3263898224171129 1103 44-45 0.30187469500765474 1103 46-47 0.27406827106187137 1103 48-49 0.29029644918535524 1103 50-51 0.32238450037110766 1103 52-53 0.23495995225980693 1103 54-55 0.21592095239670073 1103 56-57 0.2506063965736587 1103 58-59 0.07569368472157123 1103 60-61 0.1766910628030942 1103 62-63 0.05596932470892568 1103 64-65 0.09959068722185549 1103 66-67 0.14026734395813634 1103 68-69 0.1202101792258432 1103 70-71 0.2964648182658678 1103 72-73 0.3084404947797381 1103 74-75 0.0868845752403189 1103 76-77 0.22298838489650308 1103 78-79 0.19965035099530937 1103 80-81 -0.028157339924032243 1103 82-83 -0.1048688683771779 1103 84-85 0.06638028357525272 1103 86-87 0.4073115320248313 1103 88-89 0.5165994008467791 1103 90-91 0.3706671960730894 1103 92-93 0.3165133263324442 1103 94-95 0.2608665021400114 1103 96-97 0.3967606098811949 1103 98-99 0.45293333771543587 1103 100-101 0.9604681830119013 1104 1 0.3599045524013853 1104 2 0.38740347882237813 1104 3 0.37981185572790466 1104 4 0.38211348626258257 1104 5 0.4875318245347984 1104 6 0.2459354225027468 1104 7 0.42767364013273124 1104 8 0.4668847755918577 1104 9 0.5802245269952806 1104 10-11 0.5269248992791802 1104 12-13 0.30918401033819976 1104 14-15 0.42380932988480424 1104 16-17 0.22253627451254943 1104 18-19 0.6314503069924697 1104 20-21 0.7412541995421762 1104 22-23 0.530535623428122 1104 24-25 0.46544291858671727 1104 26-27 0.06930735017316181 1104 28-29 0.47224190781521 1104 30-31 0.4212947085349512 1104 32-33 0.13139254502269182 1104 34-35 0.18578066879590693 1104 36-37 0.244225112185255 1104 38-39 0.43012767998836665 1104 40-41 0.5567871846927943 1104 42-43 0.5769382039905935 1104 44-45 0.5295535234711863 1104 46-47 0.5142110440719776 1104 48-49 0.4070375630330929 1104 50-51 0.42257369154356894 1104 52-53 0.5553289079734611 1104 54-55 0.44381578430726165 1104 56-57 0.48192821348627035 1104 58-59 0.5312372400856233 1104 60-61 0.5317783391087616 1104 62-63 0.7567283472745103 1104 64-65 0.5522576147986129 1104 66-67 0.6269947976575736 1104 68-69 0.7163094166283059 1104 70-71 0.817259101324467 1104 72-73 0.6834259597045822 1104 74-75 0.4660290473019302 1104 76-77 0.6432542309507703 1104 78-79 0.581172742537305 1104 80-81 0.5116192809014848 1104 82-83 0.5873323346942847 1104 84-85 0.5087768869774969 1104 86-87 0.5286467644825485 1104 88-89 0.5626722963654629 1104 90-91 0.7002985225939113 1104 92-93 0.6400611829498111 1104 94-95 0.7324749375638255 1104 96-97 0.6769045682760684 1104 98-99 0.9155426330135974 1104 100-101 1.3455836075019505 1105 1 0.2679061524970905 1105 2 0.35220623337966117 1105 3 0.25323979908152694 1105 4 0.3447340542217887 1105 5 0.3237881987478346 1105 6 0.28237236826399226 1105 7 0.21266713507074542 1105 8 0.20448403386525627 1105 9 0.40550678686877006 1105 10-11 0.404279058780304 1105 12-13 0.3719675787011667 1105 14-15 0.15787043260496603 1105 16-17 0.27000715127526576 1105 18-19 0.2467233700541911 1105 20-21 0.2947744100968279 1105 22-23 0.051888489393931536 1105 24-25 0.39238658624533684 1105 26-27 0.3433551436860469 1105 28-29 0.16327613790000584 1105 30-31 0.12212055232373586 1105 32-33 0.06477902795251111 1105 34-35 0.04316821347148192 1105 36-37 0.1751133148441042 1105 38-39 0.16121589461054953 1105 40-41 -0.09195797389613602 1105 42-43 0.4045751841464167 1105 44-45 0.10950709078736054 1105 46-47 0.33390605386696137 1105 48-49 0.2592006830701834 1105 50-51 0.4563185136637671 1105 52-53 0.23158580558228437 1105 54-55 0.15947784259859787 1105 56-57 0.26067890511642844 1105 58-59 0.2117132685583556 1105 60-61 -0.015419437206830366 1105 62-63 0.12765146790976445 1105 64-65 0.20447326134195265 1105 66-67 0.31669773316025385 1105 68-69 0.24184290648190299 1105 70-71 0.35646499775191387 1105 72-73 0.4335270872486596 1105 74-75 0.45123694758755306 1105 76-77 0.16866846133464364 1105 78-79 0.31843908370164087 1105 80-81 0.11030611824502756 1105 82-83 0.18161367488659508 1105 84-85 0.2840370894568949 1105 86-87 0.3163295791555747 1105 88-89 0.2748279767052182 1105 90-91 0.26072101515421053 1105 92-93 0.30551369599731615 1105 94-95 0.36387363804684725 1105 96-97 0.39472096103227017 1105 98-99 0.29268743419796905 1105 100-101 0.8777225391763679 1106 1 0.15280612076543676 1106 2 0.1165698134497184 1106 3 0.2762668141186353 1106 4 0.2186977692283989 1106 5 0.2554757990645342 1106 6 0.1965625024919717 1106 7 0.3285092408203383 1106 8 0.26797044932811787 1106 9 0.593794879488236 1106 10-11 0.41808771998173455 1106 12-13 0.6088312897028061 1106 14-15 0.7930881025156609 1106 16-17 0.6553330322908053 1106 18-19 0.6150196728542809 1106 20-21 0.38615464466462157 1106 22-23 0.3541661983684463 1106 24-25 0.18583430757362862 1106 26-27 0.10221074001010066 1106 28-29 0.4089037931155488 1106 30-31 0.43265292015215095 1106 32-33 0.25898349533822795 1106 34-35 0.24308553402588728 1106 36-37 0.26948533615051673 1106 38-39 0.3070759919004189 1106 40-41 0.2663195594760168 1106 42-43 0.19917914690638838 1106 44-45 0.3048173524348812 1106 46-47 0.3466776598891883 1106 48-49 0.4747356387228834 1106 50-51 0.19797432071066368 1106 52-53 0.5176948115188793 1106 54-55 0.5180068416343246 1106 56-57 0.26398974132255404 1106 58-59 0.42391890354574 1106 60-61 0.5219497852443951 1106 62-63 0.5364714816498832 1106 64-65 0.4125468495143707 1106 66-67 0.5790796251678856 1106 68-69 0.5013446886918445 1106 70-71 0.5159918495096321 1106 72-73 0.5916772318895518 1106 74-75 0.4760090111001567 1106 76-77 0.6104081416151139 1106 78-79 0.6534658630618928 1106 80-81 0.7804830992399197 1106 82-83 0.769445470929071 1106 84-85 0.6548842476253292 1106 86-87 0.8805651928550269 1106 88-89 0.8284631381515695 1106 90-91 1.0579289761027084 1106 92-93 1.115860449048025 1106 94-95 0.874332953752166 1106 96-97 0.9318616308474574 1106 98-99 1.3480576317044175 1106 100-101 1.771791376842767 1107 1 0.04819368339437702 1107 2 0.05457452904063942 1107 3 -0.12167011485277612 1107 4 -0.04133759770352441 1107 5 0.07538443449040955 1107 6 0.11113662568678251 1107 7 0.14748949428334868 1107 8 0.1679999217713828 1107 9 0.1819027486305842 1107 10-11 0.0245863937217905 1107 12-13 0.10191263364622216 1107 14-15 -0.06318215978908626 1107 16-17 -0.010884179616056144 1107 18-19 0.22733178602845783 1107 20-21 0.13695776874360632 1107 22-23 0.29802856205839845 1107 24-25 0.2198970838777896 1107 26-27 0.001105523387643359 1107 28-29 0.025017851470984453 1107 30-31 0.11589046804487424 1107 32-33 0.007856174383320536 1107 34-35 0.21156475595338264 1107 36-37 0.21910956249887903 1107 38-39 0.19499965827235854 1107 40-41 0.15425059395877838 1107 42-43 0.22344970393557162 1107 44-45 0.28864434919290716 1107 46-47 0.24434933687121685 1107 48-49 0.15450280642108538 1107 50-51 0.05804652819666245 1107 52-53 0.2768239008203821 1107 54-55 0.2940973220351566 1107 56-57 0.2130981999137731 1107 58-59 0.3607152489627765 1107 60-61 0.29319941683885276 1107 62-63 0.31038100124905554 1107 64-65 0.3788303744185839 1107 66-67 0.42814387509420015 1107 68-69 0.3310750158359639 1107 70-71 0.41168887279285826 1107 72-73 0.28002824868884346 1107 74-75 0.2805919760279494 1107 76-77 0.33299140126733917 1107 78-79 0.3707072423722373 1107 80-81 0.33606812723874313 1107 82-83 0.5979821641209391 1107 84-85 0.4866555414655913 1107 86-87 0.3645542880510142 1107 88-89 0.2735559763478541 1107 90-91 0.3251187786373393 1107 92-93 0.33582802936043166 1107 94-95 0.5143638998175959 1107 96-97 0.6881908380387323 1107 98-99 0.8949261846074492 1107 100-101 1.1119623170137096 1108 1 0.005658656706465592 1108 2 0.24499114317205084 1108 3 0.19807320743860046 1108 4 0.17515885473392245 1108 5 0.19792588003140565 1108 6 0.054112184375348704 1108 7 -0.09768738714744529 1108 8 0.10190140001796522 1108 9 0.22679517553016382 1108 10-11 -0.016217453005168636 1108 12-13 0.2892196382196488 1108 14-15 0.20888255218399365 1108 16-17 0.3072461192808902 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4877.0 3 2014.0 4 2208.0 5 2459.0 6 2820.0 7 3356.0 8 4059.0 9 5087.0 10 6006.0 11 7436.0 12 9088.0 13 10589.0 14 11692.0 15 13844.0 16 16595.0 17 20301.0 18 18094.0 19 14416.0 20 15457.0 21 16881.0 22 18497.0 23 21153.0 24 24345.0 25 27944.0 26 32624.0 27 38392.0 28 45466.0 29 54665.0 30 65706.0 31 81181.0 32 100409.0 33 126303.0 34 164920.0 35 221620.0 36 297566.0 37 348122.0 38 218269.0 39 23437.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.23033978163605 17.50578525716678 13.734889547245514 23.528985413951663 2 16.30116478098971 19.341276538936487 38.53680049966863 25.820758180405168 3 20.10833263009141 23.269631534607367 28.28877640196641 28.333259433334813 4 13.335272796642503 15.595908050362455 35.170927127050746 35.897892025944294 5 15.06286786992315 36.38004310585353 32.41314084663559 16.14394817758772 6 34.07041957975258 33.97317894375399 16.573638678786377 15.382762797707047 7 30.999637071371033 31.430811543705715 19.133826235876903 18.43572514904635 8 28.20322313285003 32.54115708123069 18.802975346286015 20.45264443963326 9 27.471390823031548 16.448947456016235 19.35462026822549 36.72504145272673 10-11 26.671547171790998 25.137335744970557 25.720492517684878 22.47062456555357 12-13 27.568974537137493 22.428497918591138 26.416152057063364 23.586375487208002 14-15 23.988007784353755 24.01542755529614 24.910051229669435 27.086513430680675 16-17 23.917217500786904 27.49840234259498 25.08436584923837 23.500014307379747 18-19 23.795346570139706 25.675991156809253 27.357661566959795 23.171000706091245 20-21 24.60820105719211 24.72151931320651 27.105969969947065 23.564309659654313 22-23 24.076398319963555 24.631142177339246 27.342261774927657 23.95019772776954 24-25 24.307826809045956 25.05154265682196 27.206633498222168 23.43399703590992 26-27 23.889724733729388 25.2530416965807 27.361825790230913 23.495407779459 28-29 23.341368854244678 25.641264600630482 27.486534504037387 23.53083204108746 30-31 23.450135863088146 24.979239166706392 27.455737236020404 24.114887734185057 32-33 23.499142203538252 25.20613094469155 27.352505008778174 23.942221842992026 34-35 24.490180688301656 25.173372054581893 26.75350980834746 23.58293744876899 36-37 24.358635773384005 24.74615248203284 27.028158244490676 23.86705350009248 38-39 23.87272823484151 24.98478060956698 27.229908855277777 23.91258230031373 40-41 23.873398485502833 25.074194726239813 27.353805643661293 23.69860114459606 42-43 24.092982749255608 25.193203618082592 26.520156455306775 24.19365717735502 44-45 23.875018113731354 25.291726410201655 26.59250682601666 24.240748650050335 46-47 24.266870172826113 25.554211876796153 26.23246511135718 23.94645283902056 48-49 24.511058926042082 25.53809794718514 26.02582946414122 23.925013662631553 50-51 23.984331894985086 25.70951562103397 26.131939553623457 24.174212930357488 52-53 24.489471481428872 25.814390707332258 25.701559614572417 23.994578196666456 54-55 23.94522401208155 25.419402939538855 26.004269348404762 24.63110369997483 56-57 24.149094411045372 25.83281287991113 25.81989292167454 24.198199787368953 58-59 24.594127345487603 25.232239652395528 25.822799232386956 24.350833769729913 60-61 24.292884125098443 25.343832889349926 26.661052517166617 23.702230468385014 62-63 23.886628964986635 25.679568134858467 26.377900113190844 24.055902786964054 64-65 23.78080345183608 25.80113523155082 26.00264645850275 24.415414858110356 66-67 23.90908995568573 25.96317758506281 25.702564278577366 24.425168180674095 68-69 23.683178607142473 25.60885520894293 26.301124302599852 24.40684188131474 70-71 23.9512300460442 25.70919458643405 26.01802297395278 24.32155239356897 72-73 24.055792518414144 25.587531770713166 26.161883343089382 24.194792367783304 74-75 23.698066438254134 26.323873082079547 25.66740354535975 24.310656934306568 76-77 24.045731931861166 25.932804559559507 25.72291265889372 24.2985508496856 78-79 24.129368590078958 25.904560239701762 25.881416654371886 24.084654515847397 80-81 24.00428186922517 26.410294792060952 25.692740844417788 23.892682494296086 82-83 24.151037746327404 26.151831924136477 25.639382257419435 24.057748072116684 84-85 23.878497176323695 26.06082435472364 25.524837171694443 24.53584129725823 86-87 24.13411834470713 25.898487179927198 25.64403061487155 24.323363860494116 88-89 23.800978423439258 26.068179786796648 25.353418225371076 24.777423564393015 90-91 23.949426511397434 25.94547621731813 25.76810148256391 24.336995788720518 92-93 24.182847308219024 25.79737955250013 25.80715289439904 24.21262024488181 94-95 23.856644510947355 25.610843550111372 25.875496537200515 24.657015401740757 96-97 23.841416033827688 25.70527580338372 25.939843351496165 24.513464811292422 98-99 23.76672004111668 25.942533429354896 25.448170166995997 24.842576362532423 100-101 23.58807867977885 25.65544825830115 25.767291400380564 24.98918166153943 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 335.0 1 248.5 2 238.5 3 316.5 4 288.5 5 296.0 6 488.5 7 942.5 8 1527.0 9 1875.0 10 1739.0 11 1556.5 12 1643.5 13 1813.5 14 1955.5 15 2086.0 16 2253.0 17 2342.0 18 2427.0 19 2503.5 20 2670.5 21 3043.0 22 3472.5 23 4017.5 24 4559.5 25 5446.0 26 6667.0 27 8013.5 28 9477.0 29 11474.0 30 13860.0 31 16729.0 32 20685.5 33 23204.5 34 25328.5 35 27642.0 36 30585.5 37 34664.5 38 37602.0 39 39017.0 40 41096.5 41 47589.5 42 54664.5 43 59750.0 44 63317.0 45 67574.0 46 70661.0 47 76527.0 48 92605.5 49 96468.5 50 86691.0 51 81968.0 52 81815.5 53 84996.5 54 86907.0 55 83452.5 56 77725.0 57 72142.5 58 67688.5 59 61908.5 60 53853.0 61 45717.0 62 38755.5 63 31276.0 64 24646.5 65 20545.0 66 16222.5 67 13314.0 68 10188.0 69 6890.0 70 5492.5 71 4479.0 72 3705.5 73 3314.5 74 2708.5 75 2199.5 76 1513.0 77 886.0 78 568.5 79 400.0 80 269.0 81 165.0 82 102.0 83 66.0 84 50.5 85 35.0 86 27.0 87 20.0 88 9.5 89 10.5 90 12.0 91 10.0 92 7.0 93 6.0 94 7.0 95 4.5 96 2.5 97 2.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03613138668734767 2 0.024262369160765126 3 0.02240336641563774 4 0.052385744022948676 5 0.05720008446545806 6 0.049525739799675776 7 0.05090807517425767 8 0.04447306567189364 9 0.04442539893483909 10-11 0.05345824560667601 12-13 0.04776207052865748 14-15 0.04156539471156619 16-17 0.05143240928185771 18-19 0.05455458055893062 20-21 0.012727018793564417 22-23 0.021187864620746757 24-25 0.005815341920654903 26-27 0.010534348889055193 28-29 0.00619667581709129 30-31 0.00948568067385513 32-33 0.003908672438472967 34-35 0.0081271786678005 36-37 0.008723012880982354 38-39 0.011726017315418903 40-41 0.0019543362192364837 42-43 0.002264170010091048 44-45 0.0020496696933455807 46-47 4.7666737054548385E-4 48-49 0.001120168320781887 50-51 3.098337908545645E-4 52-53 0.0034796718049820317 54-55 0.007388344243455 56-57 0.017589025973128353 58-59 0.010844182679909758 60-61 0.01062968236316429 62-63 0.00545784139274579 64-65 4.766673705454838E-5 66-67 0.007388344243455 68-69 0.017946526501037466 70-71 0.006292009291200387 72-73 0.0026216705380001613 74-75 0.004123172755218435 76-77 0.007269177400818629 78-79 0.005982175500345822 80-81 0.010343681940836999 82-83 0.006292009291200387 84-85 0.01751752586754653 86-87 0.004528340020182096 88-89 0.006506509607945855 90-91 0.003837172332891145 92-93 0.0169931917599465 94-95 0.011749850683946177 96-97 0.009962348044400611 98-99 0.021616865254237692 100-101 0.03648888721525679 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2097899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.73459152424221 #Duplication Level Percentage of deduplicated Percentage of total 1 74.60516241371423 33.374314661772544 2 13.29020041738456 11.890633738940233 3 4.824735017016462 6.474976505968176 4 2.217787421643906 3.9684725757936987 5 1.2410968738303858 2.7759980846408143 6 0.7630956802199548 2.0482064129132063 7 0.5163144387341859 1.6167980860389528 8 0.36341583463585386 1.300580713270118 9 0.2625640061373921 1.0571124207172373 >10 1.6067962041477823 13.5733868667816 >50 0.169455547933958 5.332364431265055 >100 0.12735138811158647 10.92571257048904 >500 0.008911203470115451 2.6447539487341203 >1k 0.002791461327987973 2.1770494858573386 >5k 3.2209169169091997E-4 0.8396394968178166 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6434 0.3066877862089643 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5984 0.2852377545344175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5064 0.24138435644423303 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3629 0.17298258877095607 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2990 0.14252354379309967 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2762 0.13165552774466263 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2439 0.1162591716760435 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2388 0.11382816808626153 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2266 0.10801282616560665 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2242 0.10686882447629747 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2144 0.10219748424495174 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.150010558182257E-5 0.0 16-17 0.0 0.0 0.0 9.533347410909677E-5 0.0 18-19 0.0 0.0 0.0 9.533347410909677E-5 0.0 20-21 0.0 0.0 0.0 9.533347410909677E-5 0.0 22-23 0.0 0.0 0.0 1.6683357969091934E-4 0.0 24-25 0.0 0.0 0.0 3.098337908545645E-4 0.0 26-27 0.0 0.0 0.0 4.766673705454838E-4 0.0 28-29 0.0 0.0 0.0 0.001001001478145516 0.0 30-31 0.0 0.0 0.0 0.002359503484200145 0.0 32-33 0.0 0.0 0.0 0.004885840548091209 0.0 34-35 0.0 0.0 0.0 0.006792510030273145 0.0 36-37 0.0 0.0 0.0 0.008198678773382323 0.0 38-39 0.0 0.0 0.0 0.00972401435912787 0.0 40-41 0.0 0.0 0.0 0.012012017737746194 0.0 42-43 0.0 0.0 0.0 0.015062688909237289 0.0 44-45 0.0 0.0 0.0 0.018113360080728384 0.0 46-47 0.0 0.0 0.0 0.022141199361837723 0.0 48-49 0.0 0.0 0.0 0.02802804138807445 0.0 50-51 0.0 0.0 0.0 0.03622672016145677 0.0 52-53 0.0 0.0 0.0 0.05267174444527596 0.0 54-55 0.0 0.0 0.0 0.07371660885485908 0.0 56-57 0.0 0.0 0.0 0.10007631444602433 0.0 58-59 0.0 0.0 0.0 0.1317031944817172 0.0 60-61 0.0 0.0 0.0 0.17503225846430165 0.0 62-63 0.0 0.0 0.0 0.20670680523704907 0.0 64-65 0.0 0.0 0.0 0.24119368949601483 0.0 66-67 0.0 0.0 0.0 0.3024216132425822 0.0 68-69 0.0 0.0 0.0 0.3783308920019505 0.0 70-71 0.0 0.0 0.0 0.44675649304375475 0.0 72-73 0.0 0.0 0.0 0.5091999185852131 0.0 74-75 0.0 0.0 0.0 0.6246725890998566 0.0 76-77 0.0 0.0 0.0 0.7068738771504253 0.0 78-79 0.0 0.0 0.0 0.779517984421557 0.0 80-81 0.0 0.0 0.0 0.8737074568413445 0.0 82-83 4.766673705454838E-5 0.0 0.0 1.0222370095033173 0.0 84-85 4.766673705454838E-5 0.0 0.0 1.1776782390381997 0.0 86-87 4.766673705454838E-5 0.0 0.0 1.3725398601171934 0.0 88-89 4.766673705454838E-5 0.0 0.0 1.6221228953348088 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10275 0.0 25.901789 1 TACATGG 10715 0.0 24.395205 2 ACATGGG 10805 0.0 23.708164 3 TAAGGTG 745 0.0 23.60704 5 GTAAGGT 675 0.0 23.23785 4 GTGTAAG 560 0.0 22.913916 1 GAGTACT 6215 0.0 22.292688 12-13 GTATCAA 15225 0.0 21.881863 1 GTACTTT 6420 0.0 21.395765 14-15 AGTACTT 6495 0.0 21.258469 12-13 ACTTGAC 1035 0.0 21.125822 8 GATGTGC 1370 0.0 20.1235 8 ATTCGCG 625 0.0 19.759386 80-81 AGAGTAC 12250 0.0 19.555307 10-11 TACTTTT 7070 0.0 19.428688 14-15 GTACTAG 245 1.7479579E-7 19.398026 1 CATGGGA 5410 0.0 19.329079 4 CATGGGG 5840 0.0 19.208122 4 CGCGGAA 670 0.0 19.139828 82-83 TCAACGC 17395 0.0 18.936274 4 >>END_MODULE