FastQCFastQC Report
Thu 26 May 2016
SRR936465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097899
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73270.349254182398676No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66500.31698380141274674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64780.3087851226393644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40090.19109594885168446No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32520.15501222890139135No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26080.12431485023826219No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG24540.11697417273186174No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG24130.11501983651262525No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG23850.1136851678750979No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23040.10982416217367946No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC21750.10367515309364275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG98300.024.7017771
TACATGG103350.022.74992
ACATGGG102900.022.2482433
GAGTACT75750.021.22538812-13
TAAGGTG7900.021.0428735
GTAAGGT7100.020.7380264
GTACTTT78400.020.6283814-15
GTATCAA165550.020.4080351
AGTACTT78450.020.04078112-13
ATGGGCG3404.1836756E-1119.5574935
CATGGGG53300.019.5155584
AGAGTAC135900.018.99611910-11
GGTATCA130800.018.8184051
GCGCGAC2302.0594362E-618.5870239
ATGCACT17350.018.3416924
TACTTTT85750.018.33403214-15
ATACCGT2654.0117447E-717.9237376
AGGTAAG8250.017.8481242
CATGCAC19000.017.7487933
GTGTAAG5100.017.702891
Produced by FastQC (version 0.11.5)