##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936465_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2097899 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.765741820745422 31.0 31.0 34.0 30.0 34.0 2 31.913802332714777 31.0 31.0 34.0 30.0 34.0 3 32.01908623818401 31.0 31.0 34.0 30.0 34.0 4 35.78776146992777 37.0 35.0 37.0 35.0 37.0 5 35.54566544909931 37.0 35.0 37.0 33.0 37.0 6 35.6415590073688 37.0 35.0 37.0 33.0 37.0 7 35.58353619502178 37.0 35.0 37.0 33.0 37.0 8 35.551724368046315 37.0 35.0 37.0 33.0 37.0 9 37.16400265217725 39.0 37.0 39.0 33.0 39.0 10-11 37.02249345654867 39.0 37.0 39.0 33.0 39.0 12-13 36.9179174021247 39.0 37.0 39.0 33.0 39.0 14-15 37.98865483991365 40.0 37.0 41.0 33.0 41.0 16-17 37.9026387828966 40.0 37.0 41.0 33.0 41.0 18-19 38.034404897471234 40.0 37.0 41.0 33.0 41.0 20-21 38.05094978356918 40.0 37.0 41.0 33.0 41.0 22-23 37.95605484344098 40.0 37.0 41.0 33.0 41.0 24-25 37.81103189429043 40.0 37.0 41.0 32.5 41.0 26-27 37.581075638055026 39.0 37.0 41.0 32.0 41.0 28-29 37.48823584929494 39.0 36.5 41.0 32.0 41.0 30-31 37.47128150592569 39.0 36.5 41.0 32.0 41.0 32-33 37.332817738127524 39.0 36.0 41.0 31.5 41.0 34-35 37.14975506447165 39.0 36.0 41.0 31.0 41.0 36-37 36.883449107893185 39.0 35.5 41.0 30.5 41.0 38-39 36.713828930754055 39.0 35.0 40.5 30.0 41.0 40-41 36.477414546648816 39.0 35.0 40.0 30.0 41.0 42-43 36.20815778071299 38.5 35.0 40.0 30.0 41.0 44-45 35.95109535778414 38.0 35.0 40.0 29.5 41.0 46-47 35.579128451846344 38.0 35.0 40.0 28.0 41.0 48-49 35.195995136086154 38.0 34.0 40.0 27.0 41.0 50-51 34.83158793631152 38.0 34.0 40.0 26.0 41.0 52-53 34.52336480450202 38.0 33.0 40.0 25.5 41.0 54-55 34.20740655293701 37.0 33.0 40.0 24.5 41.0 56-57 33.84388786114108 37.0 33.0 40.0 23.5 41.0 58-59 33.46220838086104 36.5 32.5 39.0 23.0 41.0 60-61 33.05027672924197 36.0 32.0 39.0 22.0 40.0 62-63 32.62591430760013 35.0 31.0 39.0 22.0 40.0 64-65 32.17774640247218 35.0 31.0 38.0 20.5 40.0 66-67 31.68242393937935 35.0 30.5 38.0 19.5 40.0 68-69 31.37303726251836 35.0 30.0 37.0 18.5 39.0 70-71 30.92063726614103 34.0 30.0 36.5 17.5 39.0 72-73 30.462830908446975 34.0 29.0 36.0 14.0 39.0 74-75 29.932538458715126 34.0 29.0 35.5 10.0 38.0 76-77 28.55520689985552 32.0 27.0 34.5 8.0 36.0 78-79 29.351896349633613 33.5 29.0 35.0 7.5 37.0 80-81 29.172880343619973 34.0 29.0 35.0 7.0 36.0 82-83 28.84952159279355 33.5 29.0 35.0 2.0 36.0 84-85 28.438762304572336 33.0 27.5 35.0 2.0 35.5 86-87 28.04136710108542 33.0 27.0 35.0 2.0 35.0 88-89 27.628439452995593 33.0 26.5 35.0 2.0 35.0 90-91 27.112177707315748 32.5 25.5 34.5 2.0 35.0 92-93 26.576598063109806 32.0 25.0 34.0 2.0 35.0 94-95 25.915289296577193 32.0 24.0 34.0 2.0 35.0 96-97 25.06164119435683 31.5 20.0 34.0 2.0 35.0 98-99 24.0017898859764 31.0 9.5 34.0 2.0 35.0 100-101 20.691016583734488 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3943365959156253 1101 2 0.446634913677169 1101 3 0.44180513034717706 1101 4 0.21899387816579008 1101 5 0.29673961830251727 1101 6 0.24993704221755308 1101 7 0.22674067903474082 1101 8 0.29854281501094704 1101 9 0.3857935820309777 1101 10-11 0.4118274859117719 1101 12-13 0.426540482810843 1101 14-15 0.5147272860526257 1101 16-17 0.6018828144793389 1101 18-19 0.5675621457621034 1101 20-21 0.4444408707799141 1101 22-23 0.4061847130353513 1101 24-25 0.26891524003470835 1101 26-27 0.3292896508004688 1101 28-29 0.2941853006023436 1101 30-31 0.4396321217215231 1101 32-33 0.5000787922052297 1101 34-35 0.3727112988600396 1101 36-37 0.4799348170470168 1101 38-39 0.465995420731808 1101 40-41 0.5218694414436982 1101 42-43 0.6819801863668431 1101 44-45 0.6062914393608949 1101 46-47 0.4915751946957059 1101 48-49 0.4171651686220059 1101 50-51 0.5631039797727411 1101 52-53 0.5323736619829091 1101 54-55 0.5148709739682289 1101 56-57 0.45732102551966847 1101 58-59 0.41236266370645325 1101 60-61 0.3019840044854334 1101 62-63 0.4108936656083273 1101 64-65 0.46765086283341617 1101 66-67 0.5471582216171278 1101 68-69 0.42714571416933467 1101 70-71 0.3958202219656215 1101 72-73 0.47689080907354864 1101 74-75 0.4313514162296741 1101 76-77 0.2492136038558712 1101 78-79 0.02349888897690633 1101 80-81 0.15822246992022215 1101 82-83 0.3696756104043395 1101 84-85 0.4253506086325487 1101 86-87 0.3618677165123465 1101 88-89 0.26251374152598217 1101 90-91 0.33445745926713144 1101 92-93 0.4497150329288395 1101 94-95 0.5308686659750599 1101 96-97 0.4463793675579737 1101 98-99 0.533523548190626 1101 100-101 0.9062268092091372 1102 1 0.22717818748370888 1102 2 0.27971467839262587 1102 3 0.18357122826863304 1102 4 0.163437104889411 1102 5 0.1738407086149607 1102 6 0.20754241768959503 1102 7 0.23264647284607065 1102 8 0.2058425773466297 1102 9 0.2460051236237959 1102 10-11 0.28791130094067796 1102 12-13 0.30951145504263167 1102 14-15 0.3878857007471126 1102 16-17 0.36296060570423805 1102 18-19 0.24348599141494276 1102 20-21 0.19786796122387074 1102 22-23 0.25263025769056213 1102 24-25 0.2912956876828048 1102 26-27 -0.026108620888358303 1102 28-29 0.15141625301311734 1102 30-31 0.25350176409779834 1102 32-33 0.32089136774740723 1102 34-35 0.20276467939223153 1102 36-37 0.37030667245693394 1102 38-39 0.3387480408321366 1102 40-41 0.2205210135038982 1102 42-43 0.4292247559408011 1102 44-45 0.5643375427934103 1102 46-47 0.41167691048174504 1102 48-49 0.2754013604506156 1102 50-51 0.08152932452691175 1102 52-53 0.07160549584960307 1102 54-55 0.17063768014124037 1102 56-57 0.22237975271611532 1102 58-59 0.05114451275986909 1102 60-61 0.1791048610163486 1102 62-63 0.17163611608710028 1102 64-65 0.15371497426948366 1102 66-67 0.13546409621554645 1102 68-69 -0.011230653418135006 1102 70-71 -0.09658818020372806 1102 72-73 -0.32775994728764957 1102 74-75 -0.08322881191554288 1102 76-77 -0.34903242802684176 1102 78-79 -0.24616321436792887 1102 80-81 0.0837097952729593 1102 82-83 -0.07872751398897648 1102 84-85 -0.09174942095806671 1102 86-87 -0.18829287912014436 1102 88-89 -0.1289130624056547 1102 90-91 0.017817610727245636 1102 92-93 0.17695951639253948 1102 94-95 0.24913224086593289 1102 96-97 0.36881907472047004 1102 98-99 0.3931886810509262 1102 100-101 1.1398959007928795 1103 1 0.18729382431213537 1103 2 0.16891978518419037 1103 3 0.19415178195462346 1103 4 0.1583568645698037 1103 5 0.021969926711790322 1103 6 0.1792346656534889 1103 7 0.19655511650989865 1103 8 0.22827085040453454 1103 9 0.1790730345968683 1103 10-11 0.13214080554391927 1103 12-13 0.12558593513228544 1103 14-15 0.1529315325110403 1103 16-17 0.3371895970349428 1103 18-19 0.13871452504548643 1103 20-21 0.24755283890291935 1103 22-23 0.23426310883072432 1103 24-25 0.17935017638617978 1103 26-27 0.32373571860321704 1103 28-29 0.35847097172432996 1103 30-31 0.22632137851820744 1103 32-33 0.2253777170135436 1103 34-35 0.12748515811728112 1103 36-37 0.19237206569316356 1103 38-39 0.23157509577468005 1103 40-41 0.18836323507091635 1103 42-43 0.1623028744766586 1103 44-45 0.1556840380296265 1103 46-47 0.18156766833443072 1103 48-49 0.046012243526362795 1103 50-51 0.059722477857242495 1103 52-53 -0.07521051640102172 1103 54-55 0.005623744599006386 1103 56-57 -0.05194589207768985 1103 58-59 0.0015397133143508768 1103 60-61 -0.09844687531131058 1103 62-63 -0.07543919527250864 1103 64-65 -0.15117373435225545 1103 66-67 0.13835945499690538 1103 68-69 0.05168652523466477 1103 70-71 -0.005444678465551078 1103 72-73 -0.01602474265785503 1103 74-75 -0.10328329598788599 1103 76-77 -0.2646128929514191 1103 78-79 -0.19397844860544566 1103 80-81 0.01602950540133108 1103 82-83 0.12555688376096086 1103 84-85 0.04621215769844156 1103 86-87 -0.31773268653102704 1103 88-89 -0.09784106763563116 1103 90-91 0.17181934196076654 1103 92-93 0.17901154524694718 1103 94-95 0.1550444650880749 1103 96-97 0.1499334241224517 1103 98-99 0.5720763152736232 1103 100-101 0.7820188461590689 1104 1 0.12166452611636558 1104 2 0.24833924769686533 1104 3 0.12862540526192134 1104 4 0.1418297785278071 1104 5 0.026906960201728225 1104 6 0.11922296233083785 1104 7 0.14672304601901942 1104 8 0.04314423061387629 1104 9 0.1650331868529591 1104 10-11 0.1455829747604156 1104 12-13 0.19573089075561967 1104 14-15 0.1689554885727702 1104 16-17 0.18432670465847423 1104 18-19 0.08447047125535789 1104 20-21 -0.0010490656152768452 1104 22-23 -0.018062973585500686 1104 24-25 0.09860769276048131 1104 26-27 0.15017685484155407 1104 28-29 0.11588574161856258 1104 30-31 0.06492710260955192 1104 32-33 0.020330329846174777 1104 34-35 0.15954435961951674 1104 36-37 0.273840644529713 1104 38-39 0.22459609590074336 1104 40-41 0.3156135375321867 1104 42-43 0.2506014333874518 1104 44-45 0.22768797802281426 1104 46-47 0.21952180711475933 1104 48-49 0.2574604262186142 1104 50-51 0.1821253092334345 1104 52-53 0.06636158603880205 1104 54-55 0.0919293518452804 1104 56-57 0.40102857699223904 1104 58-59 0.13292249287230362 1104 60-61 0.19390983895262792 1104 62-63 0.1430443401093413 1104 64-65 0.20349244812549472 1104 66-67 0.09462242602271331 1104 68-69 0.293076304207446 1104 70-71 0.21865278274131938 1104 72-73 0.002828708034840588 1104 74-75 0.04685377588594264 1104 76-77 0.0546173407575381 1104 78-79 0.09772997308295572 1104 80-81 0.4056713122287725 1104 82-83 0.2434669187015892 1104 84-85 0.20789487723452282 1104 86-87 0.02181071454485206 1104 88-89 0.17331692157548417 1104 90-91 0.26422300122473885 1104 92-93 0.2988707855093864 1104 94-95 0.6320066061563878 1104 96-97 0.702265245306215 1104 98-99 0.6770931270401661 1104 100-101 0.7620504752352417 1105 1 0.04035910224054362 1105 2 0.15445550137932784 1105 3 0.012850256094125712 1105 4 0.024107223160008573 1105 5 -0.06227108409250803 1105 6 0.012774726174654916 1105 7 0.08041141616452308 1105 8 0.04066707310165185 1105 9 -0.043974080871464594 1105 10-11 0.06164823473858405 1105 12-13 -0.08853919642716335 1105 14-15 0.02523418187779214 1105 16-17 0.09158003569883277 1105 18-19 0.025880050893768214 1105 20-21 0.06359816562098786 1105 22-23 0.001299866808686545 1105 24-25 -0.048103512395712755 1105 26-27 0.07108352589477818 1105 28-29 -0.04271346431524137 1105 30-31 0.024046526575098426 1105 32-33 0.03794521522249994 1105 34-35 -8.361290895209095E-6 1105 36-37 -0.15814145533420287 1105 38-39 -0.051596311869438694 1105 40-41 0.11100952383417706 1105 42-43 0.02434808739293004 1105 44-45 -0.0930534495018307 1105 46-47 0.015258000681477313 1105 48-49 -0.022202095651849163 1105 50-51 0.06542431029517815 1105 52-53 0.008292692198430984 1105 54-55 0.07544687065014699 1105 56-57 -0.06941675805584424 1105 58-59 -0.08121442746685403 1105 60-61 -0.021717450903956603 1105 62-63 0.11398073256646057 1105 64-65 -0.12976486819996325 1105 66-67 0.27365145205791563 1105 68-69 0.32596630884323474 1105 70-71 0.281995823820413 1105 72-73 0.2052023079510974 1105 74-75 0.16343869311463877 1105 76-77 0.004769499391326093 1105 78-79 0.22897726264045914 1105 80-81 0.4435341376687987 1105 82-83 0.36781454323240226 1105 84-85 0.26111394828176415 1105 86-87 0.1851075846380148 1105 88-89 0.2881893999293297 1105 90-91 0.3275916513122219 1105 92-93 0.33977098888603763 1105 94-95 0.29647345277151516 1105 96-97 0.2203397843440662 1105 98-99 0.4852824019126807 1105 100-101 0.5349974552415233 1106 1 0.1944521006354769 1106 2 0.215574365694156 1106 3 0.11198549147830761 1106 4 0.10222678372279859 1106 5 -0.028135294174546743 1106 6 0.010111423679560971 1106 7 0.09919740309600655 1106 8 0.08409245904175577 1106 9 0.07937387058834133 1106 10-11 0.18650767583015693 1106 12-13 0.03398542641284763 1106 14-15 0.21185193404432567 1106 16-17 0.07738916979434407 1106 18-19 0.06941865414641768 1106 20-21 0.0671914674691223 1106 22-23 0.1388398394516912 1106 24-25 -0.05267340983778013 1106 26-27 -0.026744704187443347 1106 28-29 0.1727877957087216 1106 30-31 0.19435963791007538 1106 32-33 0.10449812544656822 1106 34-35 0.1605761481161636 1106 36-37 0.1718681411808589 1106 38-39 0.3621842760595939 1106 40-41 0.39693907647944826 1106 42-43 0.1261737743216429 1106 44-45 0.12143482364918867 1106 46-47 0.08447344321483996 1106 48-49 0.052018206753899676 1106 50-51 0.020148745517936106 1106 52-53 -0.2055890596353862 1106 54-55 -0.2549705392458179 1106 56-57 -0.11788609079766843 1106 58-59 -0.16363663099924253 1106 60-61 -0.11296925698965055 1106 62-63 -0.14982041932341872 1106 64-65 0.0850938643206689 1106 66-67 0.14594925517993929 1106 68-69 0.22368236136374975 1106 70-71 0.04949771847747897 1106 72-73 -0.10623425800607222 1106 74-75 -0.06284651592919133 1106 76-77 -0.33658570413797406 1106 78-79 -0.14666102884097398 1106 80-81 0.025417596234344586 1106 82-83 0.3153078965289531 1106 84-85 0.054217589104077035 1106 86-87 0.128070319968284 1106 88-89 0.19917652396312846 1106 90-91 0.3400359283696197 1106 92-93 0.32332424877445476 1106 94-95 0.46080048752537905 1106 96-97 0.3989930451567041 1106 98-99 0.8325772783286673 1106 100-101 1.0789702507387062 1107 1 -0.04539464265954152 1107 2 -0.0023448798112966074 1107 3 0.02199138596696315 1107 4 -0.019848076283096816 1107 5 -0.05927293048459603 1107 6 0.010023006349761943 1107 7 -0.06963548815067355 1107 8 -0.18248579029511802 1107 9 -0.12226185001289736 1107 10-11 -0.09146904093966413 1107 12-13 -0.22590257830274396 1107 14-15 -0.017635245442853886 1107 16-17 -0.07646435216262404 1107 18-19 -0.06816607912797679 1107 20-21 -0.1905450488880831 1107 22-23 -0.21481474351323726 1107 24-25 -0.16635599162380288 1107 26-27 -0.31235478376304826 1107 28-29 -0.21680106370772734 1107 30-31 -0.23372023241118 1107 32-33 -0.24441876226342174 1107 34-35 -0.17427501604534967 1107 36-37 -0.21383772383535415 1107 38-39 -0.08325486334506138 1107 40-41 -0.039090985412677526 1107 42-43 -0.22107055223303007 1107 44-45 -0.14914430986097926 1107 46-47 0.02702428317947181 1107 48-49 0.16471346169053902 1107 50-51 0.04573082627242542 1107 52-53 -0.16053306199318484 1107 54-55 -0.11960360732716424 1107 56-57 -0.29816165814219886 1107 58-59 -0.32711290568241225 1107 60-61 -0.27808862038488513 1107 62-63 -0.1856220697802371 1107 64-65 0.02345961734159374 1107 66-67 -0.06897116922324287 1107 68-69 -0.17248622101173083 1107 70-71 -0.2292526499280605 1107 72-73 -0.12939223030197766 1107 74-75 -0.27276399308804855 1107 76-77 -0.1852078674153077 1107 78-79 -0.1076174096249396 1107 80-81 0.09525991866287242 1107 82-83 0.006488267881739063 1107 84-85 -0.026153763681069364 1107 86-87 0.09223182895738091 1107 88-89 -0.016495447743320568 1107 90-91 0.0685726215614828 1107 92-93 0.1423339746807315 1107 94-95 0.5202021969270874 1107 96-97 0.5443216628991152 1107 98-99 0.8611802845178822 1107 100-101 1.1236283703968297 1108 1 -0.054937337584444634 1108 2 -0.08385208764840968 1108 3 0.030802217017395606 1108 4 -0.049617035779370156 1108 5 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4524.0 12 6235.0 13 8593.0 14 10860.0 15 14020.0 16 17741.0 17 21967.0 18 20083.0 19 15026.0 20 14702.0 21 15637.0 22 16359.0 23 18916.0 24 22172.0 25 26678.0 26 32625.0 27 40028.0 28 49307.0 29 60263.0 30 72737.0 31 89358.0 32 109483.0 33 137216.0 34 177776.0 35 236524.0 36 319631.0 37 361947.0 38 164615.0 39 5762.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65187487095466 18.455928599596305 14.4841214020488 23.408075127400245 2 16.819415154277085 20.106121507266717 37.36728852922894 25.70717480922726 3 20.107216880127982 23.34045506634557 28.881249153877103 27.67107889964935 4 13.948790402538172 16.23132407449778 34.80119053066703 35.018694992297014 5 15.037368552262349 36.47551083542631 32.545699553884454 15.941421058426883 6 34.15803615733354 34.49570925412093 16.022645642973142 15.323608945572378 7 30.830750193407784 31.730173854890058 18.9330372911184 18.506038660583755 8 28.408898617140295 32.75143369628376 18.46804827115128 20.371619415424668 9 27.129286112312073 16.21864469878938 19.30847329004777 37.34359589885078 10-11 26.708723219216264 25.310699157415108 25.542384382018213 22.438193241350415 12-13 27.50267413326622 22.486815235981535 26.443269287603272 23.567241343148975 14-15 23.88982463282147 24.044482568031974 25.005821420970133 27.059871378176425 16-17 23.825811518262217 27.474941517296937 25.326615320263556 23.372631644177293 18-19 23.635980569131306 25.47868129018604 27.75128831273574 23.13404982794691 20-21 24.359299470565553 24.45968561880243 27.759177157718266 23.42183775291375 22-23 23.925370722492364 24.455505435769638 27.940742555638764 23.678381286099235 24-25 24.23070103018089 24.80430281178559 27.866340560297896 23.098655597735622 26-27 23.806042844336947 25.097631880202314 27.874560224539618 23.221765050921118 28-29 23.25419042681873 25.360123463410854 28.089284543834875 23.29640156593554 30-31 23.378156467854378 24.80331287612465 28.00526723470178 23.813263421319192 32-33 23.471131974381905 24.93567352370856 28.008805982386132 23.584388519523408 34-35 24.424515853463202 24.750990603095183 27.4577253693548 23.366768174086815 36-37 24.44383900834334 24.45775927145538 27.569104929465503 23.529296790735778 38-39 23.791915042614317 24.646751780095492 27.917865692143888 23.643467485146306 40-41 23.748269661978703 24.817361153482413 27.879392103175814 23.554977081363067 42-43 23.998914144224813 24.919079196209324 27.105424536002815 23.97658212356305 44-45 23.839188741181257 24.95639040543636 27.071244218257945 24.13317663512444 46-47 24.257023996644257 25.38400946662075 26.638360549073536 23.720605987661454 48-49 24.662320864355628 25.30863800305689 26.314316813612177 23.71472431897531 50-51 24.032784556585273 25.491496645658135 26.478875320681183 23.99684347707541 52-53 24.575611913945934 25.630790468690712 26.00117072575463 23.792426891608716 54-55 24.004717219471782 25.257359215118747 26.283244813966327 24.454678751443147 56-57 24.244787390254732 25.67909967707715 26.0949024712929 23.98121046137522 58-59 24.878115049478524 25.0146099872252 26.03790493259862 24.069370030697655 60-61 24.353721034770906 25.18670599008398 27.013104574898573 23.44646840024654 62-63 23.99621412096651 25.461657249240933 26.652264245966776 23.88986438382578 64-65 23.86523739958436 25.576858858803025 26.302099161541452 24.255804580071157 66-67 24.031313160202874 25.67250123937002 26.057039621706135 24.23914597872097 68-69 23.867903752155794 25.30343201606991 26.534892655948944 24.293771575825353 70-71 24.09968172889327 25.47038772637551 26.330892317401666 24.099038227329558 72-73 24.193677934499895 25.34484307807736 26.42765332244612 24.033825664976625 74-75 23.906370253985955 26.13337426562019 25.78838945195942 24.171866028434433 76-77 24.153652365931354 25.733220042877182 25.974299909073682 24.138827682117785 78-79 24.182492776448754 25.649988523830984 26.112634249130828 24.05488445058943 80-81 24.133901357518695 26.18762626334852 25.861498580892917 23.816973798239868 82-83 24.206132236691886 25.894759221450673 25.857410552679262 24.041697989178182 84-85 23.95292459874911 25.867802850008115 25.792246912038348 24.387025639204428 86-87 24.120034111325143 25.642292513670036 25.853510067750733 24.38416330725409 88-89 23.888815263839337 25.91597241952676 25.483216031150068 24.71199628548383 90-91 24.01500826283958 25.8218921270978 25.87883695259135 24.284262657471274 92-93 24.253924324448 25.543895090131596 25.94775575498468 24.254424830435724 94-95 23.89151472351594 25.427014257205528 26.019453712524452 24.662017306754077 96-97 23.936467245362397 25.448566253956077 26.101654600111495 24.513311900570027 98-99 23.87650679368033 25.598260489877166 25.543728001098277 24.981504715344222 100-101 23.426392418368323 25.5842410669809 25.859218698383597 25.13014781626718 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 466.0 1 342.5 2 305.5 3 408.0 4 347.0 5 301.5 6 517.5 7 1066.0 8 1694.5 9 2021.5 10 1879.5 11 1644.5 12 1650.5 13 1826.5 14 2092.5 15 2333.0 16 2523.0 17 2682.0 18 2822.0 19 2969.0 20 3156.5 21 3432.5 22 3845.0 23 4342.5 24 4935.0 25 5861.0 26 6967.0 27 8348.5 28 9860.0 29 11721.0 30 14023.0 31 17004.5 32 20969.0 33 23176.5 34 24931.0 35 27302.0 36 30339.0 37 34535.0 38 37710.5 39 38851.5 40 40636.0 41 47386.5 42 54885.0 43 59626.0 44 62558.5 45 66585.0 46 70186.0 47 76796.0 48 93415.0 49 97018.0 50 86919.5 51 81920.0 52 81689.5 53 85356.5 54 87072.0 55 83296.5 56 77098.5 57 71520.5 58 67637.5 59 62241.5 60 53958.5 61 45258.0 62 38093.5 63 30990.5 64 24180.5 65 19979.5 66 15987.5 67 13057.5 68 9845.5 69 6392.0 70 4950.5 71 4081.0 72 3602.5 73 3260.0 74 2680.5 75 2254.0 76 1551.5 77 916.0 78 631.0 79 480.0 80 334.5 81 230.5 82 157.0 83 92.5 84 56.5 85 29.5 86 13.5 87 7.5 88 4.5 89 2.5 90 3.0 91 2.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036608054057893157 2 0.005481674761273064 3 0.004528340020182096 4 5.243341076000323E-4 5 5.720008446545806E-4 6 5.720008446545806E-4 7 0.0 8 0.0 9 0.0022880033786183223 10-11 4.5283400201820965E-4 12-13 0.0010963349522546126 14-15 0.002073503061872855 16-17 0.0026931706435819837 18-19 0.0 20-21 0.0 22-23 0.0017160025339637416 24-25 7.150010558182257E-4 26-27 0.004552173388709371 28-29 0.005314841181582145 30-31 0.0011440016893091611 32-33 3.3366715938183873E-4 34-35 0.008913679829200548 36-37 0.011320850050455241 38-39 0.00898517993478237 40-41 0.0020496696933455807 42-43 6.911676872909515E-4 44-45 0.001120168320781887 46-47 7.150010558182257E-5 48-49 5.958342131818548E-4 50-51 0.001001001478145516 52-53 0.0026216705380001613 54-55 0.0025263370638910643 56-57 0.0014061687431091772 58-59 9.056680040364193E-4 60-61 0.003908672438472967 62-63 0.004933507285145758 64-65 0.008603846038345983 66-67 0.0028123374862183545 68-69 0.003098337908545645 70-71 9.533347410909677E-5 72-73 0.0011678350578364354 74-75 0.005982175500345822 76-77 0.0022165032730364995 78-79 0.0035035051735093064 80-81 0.004814340442509387 82-83 0.0047666737054548386 84-85 0.005505508129800338 86-87 0.002955337697382 88-89 0.008723012880982354 90-91 0.012131184580382563 92-93 0.0010486682152000646 94-95 0.002264170010091048 96-97 9.771681096182418E-4 98-99 0.003193671382654742 100-101 0.004385339809018451 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2097899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.187421987494346 #Duplication Level Percentage of deduplicated Percentage of total 1 72.50958086304948 30.589922860058135 2 14.361495185714562 12.117489155422174 3 5.151419370996195 6.519753085163075 4 2.40146511117627 4.052464881337533 5 1.359866037732331 2.8684621190137882 6 0.8466203228030479 2.143003729276852 7 0.5820295705392112 1.7188028971076468 8 0.39476981623333673 1.3323456660289101 9 0.3018457288497245 1.146068382429551 >10 1.7464652701947112 13.957775295528222 >50 0.18576866547638402 5.5027684664462395 >100 0.145405358367974 11.829682149992644 >500 0.009072614609767595 2.552991818329021 >1k 0.003855861209151229 2.689825022362322 >5k 3.4022304786628484E-4 0.9786444715039095 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7327 0.349254182398676 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6650 0.31698380141274674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6478 0.3087851226393644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4009 0.19109594885168446 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3252 0.15501222890139135 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2608 0.12431485023826219 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2454 0.11697417273186174 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2413 0.11501983651262525 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2385 0.1136851678750979 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2304 0.10982416217367946 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2175 0.10367515309364275 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.383336852727419E-5 0.0 16-17 0.0 0.0 0.0 4.766673705454838E-5 0.0 18-19 0.0 0.0 0.0 4.766673705454838E-5 0.0 20-21 0.0 0.0 0.0 4.766673705454838E-5 0.0 22-23 0.0 0.0 0.0 1.1916684263637095E-4 0.0 24-25 0.0 0.0 0.0 2.383336852727419E-4 0.0 26-27 0.0 0.0 0.0 3.8133389643638707E-4 0.0 28-29 0.0 0.0 0.0 9.295013725636935E-4 0.0 30-31 0.0 0.0 0.0 0.002264170010091048 0.0 32-33 0.0 0.0 0.0 0.004838173811036661 0.0 34-35 0.0 0.0 0.0 0.006792510030273145 0.0 36-37 0.0 0.0 0.0 0.008270178878964144 0.0 38-39 0.0 0.0 0.0 0.009771681096182419 0.0 40-41 0.0 0.0 0.0 0.012107351211855289 0.0 42-43 0.0 0.0 0.0 0.015062688909237289 0.0 44-45 0.0 0.0 0.0 0.018280193660419306 0.0 46-47 0.0 0.0 0.0 0.02235569967858319 0.0 48-49 0.0 0.0 0.0 0.028171041599238097 0.0 50-51 0.0 0.0 0.0 0.03629822026703859 0.0 52-53 0.0 0.0 0.0 0.05271941118233051 0.0 54-55 0.0 0.0 0.0 0.07335910832694997 0.0 56-57 0.0 0.0 0.0 0.09971881391811521 0.0 58-59 0.0 0.0 0.0 0.1313933606908626 0.0 60-61 0.0 0.0 0.0 0.17438875751406527 0.0 62-63 0.0 0.0 0.0 0.20582497060153992 0.0 64-65 0.0 0.0 0.0 0.23988285422701475 0.0 66-67 0.0 0.0 0.0 0.3003242768121821 0.0 68-69 0.0 0.0 0.0 0.37568538809542307 0.0 70-71 0.0 0.0 0.0 0.4433721547128818 0.0 72-73 0.0 0.0 0.0 0.5052674127782129 0.0 74-75 0.0 0.0 0.0 0.6202634159223108 0.0 76-77 0.0 0.0 0.0 0.7024170372358249 0.0 78-79 0.0 0.0 0.0 0.7746798106105204 0.0 80-81 0.0 0.0 0.0 0.8677252813409988 0.0 82-83 0.0 0.0 0.0 1.015158499050717 0.0 84-85 0.0 0.0 0.0 1.1681210582587627 0.0 86-87 0.0 0.0 0.0 1.360408675536811 0.0 88-89 0.0 0.0 0.0 1.6079420410610807 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9830 0.0 24.701777 1 TACATGG 10335 0.0 22.7499 2 ACATGGG 10290 0.0 22.248243 3 GAGTACT 7575 0.0 21.225388 12-13 TAAGGTG 790 0.0 21.042873 5 GTAAGGT 710 0.0 20.738026 4 GTACTTT 7840 0.0 20.62838 14-15 GTATCAA 16555 0.0 20.408035 1 AGTACTT 7845 0.0 20.040781 12-13 ATGGGCG 340 4.1836756E-11 19.557493 5 CATGGGG 5330 0.0 19.515558 4 AGAGTAC 13590 0.0 18.996119 10-11 GGTATCA 13080 0.0 18.818405 1 GCGCGAC 230 2.0594362E-6 18.587023 9 ATGCACT 1735 0.0 18.341692 4 TACTTTT 8575 0.0 18.334032 14-15 ATACCGT 265 4.0117447E-7 17.923737 6 AGGTAAG 825 0.0 17.848124 2 CATGCAC 1900 0.0 17.748793 3 GTGTAAG 510 0.0 17.70289 1 >>END_MODULE