FastQCFastQC Report
Thu 26 May 2016
SRR936464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162364
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63750.29481622890503173No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62970.2912090656337231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50400.23307824214609563No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36720.16981414784929827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30280.14003192801951939No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27220.12588074903207785No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG24280.11228451824022229No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24260.11219202687429129No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG23590.10909356611560311No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG22370.10345159279381269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG107650.027.5899831
TACATGG112300.025.855142
ACATGGG111500.024.7637753
GTAAGGT6850.022.8954434
GTATCAA160150.022.6403471
GAGTACT69300.022.5612512-13
GTACTTT71550.021.51669714-15
TAAGGTG7150.021.271585
AGTACTT71250.021.21010412-13
CGTATAG1150.003714803820.6612991
CATGGGG55300.020.3680234
AGAGTAC129700.020.26567310-11
GTACTAG2351.12775524E-720.2216971
CGCGGAA7150.019.92550582-83
CATGGGA56500.019.7671994
TCAACGC187200.019.3198834
TATCAAC188300.019.3061892
ATTCGCG6650.019.28241380-81
GGTATCA122500.019.2023581
ATCAACG188550.019.13073
Produced by FastQC (version 0.11.5)