##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936464_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2162364 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.228390317263884 31.0 31.0 34.0 28.0 34.0 2 31.272759812871467 31.0 31.0 34.0 28.0 34.0 3 31.22045733280798 31.0 31.0 34.0 27.0 34.0 4 34.883111724020566 37.0 35.0 37.0 32.0 37.0 5 34.914822388830004 37.0 35.0 37.0 32.0 37.0 6 34.97697519936514 37.0 35.0 37.0 32.0 37.0 7 34.831954287067305 37.0 35.0 37.0 32.0 37.0 8 34.80960004883544 37.0 35.0 37.0 32.0 37.0 9 36.27474190284337 39.0 35.0 39.0 32.0 39.0 10-11 36.138451944261 39.0 35.0 39.0 31.0 39.0 12-13 36.014242282982885 39.0 35.0 39.0 30.0 39.0 14-15 36.972305772756116 39.0 36.0 41.0 31.0 41.0 16-17 36.880545088615975 39.0 36.0 41.0 31.0 41.0 18-19 36.71389807636457 39.0 36.0 41.0 30.0 41.0 20-21 36.682794848600885 39.0 36.0 41.0 30.0 41.0 22-23 36.676026330442056 39.0 36.0 40.5 30.0 41.0 24-25 36.778443638536345 39.0 36.0 41.0 30.0 41.0 26-27 36.70500086941884 39.0 36.0 41.0 30.0 41.0 28-29 36.581076312776204 39.0 36.0 41.0 30.0 41.0 30-31 36.377670225734434 39.0 35.5 40.5 30.0 41.0 32-33 36.22514595137544 39.0 35.0 40.0 30.0 41.0 34-35 36.01768550530808 38.0 35.0 40.0 29.0 41.0 36-37 35.79636985262426 38.0 35.0 40.0 27.5 41.0 38-39 35.591485522326494 38.0 35.0 40.0 27.0 41.0 40-41 35.34084340101852 38.0 34.0 40.0 26.5 41.0 42-43 35.03929403190212 38.0 34.0 40.0 26.0 41.0 44-45 34.8797001799882 38.0 34.0 40.0 25.0 41.0 46-47 34.71904175245241 38.0 33.5 40.0 24.0 41.0 48-49 34.45646523896994 38.0 33.0 40.0 24.0 41.0 50-51 33.43211619320336 37.0 32.0 39.0 22.5 40.5 52-53 33.52642062113502 37.0 32.5 39.5 23.0 40.0 54-55 33.92733785801096 38.0 33.0 40.0 23.0 41.0 56-57 33.74208597627411 37.0 33.0 40.0 22.0 41.0 58-59 33.48237207056721 37.0 33.0 40.0 22.0 41.0 60-61 33.14424560342292 36.0 32.0 39.5 21.0 41.0 62-63 32.784603332278934 36.0 32.0 39.0 20.0 41.0 64-65 32.391674343450035 35.0 31.0 39.0 19.0 40.5 66-67 32.00432512749935 35.0 31.0 38.5 18.0 40.0 68-69 31.565948193736116 35.0 31.0 38.0 16.0 40.0 70-71 31.15395997158665 35.0 30.0 37.0 13.0 39.5 72-73 30.738070694850634 35.0 30.0 36.5 9.5 39.0 74-75 30.332170485635167 34.0 30.0 36.0 7.5 39.0 76-77 29.888172851564306 34.0 29.0 35.5 7.0 37.5 78-79 29.435383219476464 34.0 29.0 35.0 2.0 37.0 80-81 29.0012615822313 34.0 29.0 35.0 2.0 36.5 82-83 28.629943201052182 34.0 28.5 35.0 2.0 36.0 84-85 28.274796703977685 34.0 27.0 35.0 2.0 36.0 86-87 27.931187348661005 33.0 27.0 35.0 2.0 35.0 88-89 27.581555880508553 33.0 26.0 35.0 2.0 35.0 90-91 27.219504671738893 33.0 25.5 35.0 2.0 35.0 92-93 26.78758779742911 33.0 25.0 35.0 2.0 35.0 94-95 26.265529300339814 33.0 24.5 35.0 2.0 35.0 96-97 25.616709073957946 32.0 21.5 35.0 2.0 35.0 98-99 24.823756546076424 32.0 13.0 34.5 2.0 35.0 100-101 22.173939956455065 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.49509553283703056 1101 2 0.5194599313930937 1101 3 0.545575707477699 1101 4 0.5566976786164801 1101 5 0.5975744574331827 1101 6 0.5527151784842772 1101 7 0.5333660325500205 1101 8 0.5328413340035709 1101 9 0.7277636916397796 1101 10-11 0.7623127035051098 1101 12-13 0.7610916539715475 1101 14-15 0.9859797790070601 1101 16-17 0.9978360256559782 1101 18-19 1.218560176251188 1101 20-21 1.181382422962379 1101 22-23 0.9902279034347856 1101 24-25 0.9831238791734691 1101 26-27 0.7509277255132574 1101 28-29 0.7640240379503851 1101 30-31 0.7448290885980668 1101 32-33 0.6754857402636034 1101 34-35 0.7692736214126228 1101 36-37 0.645428528299199 1101 38-39 0.7767502978889169 1101 40-41 0.7346314440185395 1101 42-43 0.7599272005357776 1101 44-45 0.6240678133621671 1101 46-47 0.7481929913889971 1101 48-49 0.8484179819572759 1101 50-51 0.7550238253266315 1101 52-53 0.8501141595106887 1101 54-55 0.8556777798750304 1101 56-57 1.202714654142703 1101 58-59 0.8738157479260025 1101 60-61 0.6804714284880333 1101 62-63 0.7695685117385196 1101 64-65 0.6723464970040922 1101 66-67 0.8350097032580663 1101 68-69 0.8229436913366044 1101 70-71 0.679118860166529 1101 72-73 0.7943100807041219 1101 74-75 0.5888863295845113 1101 76-77 0.766411521511678 1101 78-79 0.7892557630593338 1101 80-81 0.7208784636937544 1101 82-83 0.8127263277993748 1101 84-85 0.8162174228783705 1101 86-87 0.7697310869204479 1101 88-89 0.9334337090350004 1101 90-91 0.7830456897238633 1101 92-93 0.7310060174310244 1101 94-95 0.9274242204048058 1101 96-97 0.9911373303379563 1101 98-99 0.9236795927886341 1101 100-101 1.2508482431855583 1102 1 0.4315704537459766 1102 2 0.48960402572418715 1102 3 0.6285494496551145 1102 4 0.4254716756767749 1102 5 0.42907753353567557 1102 6 0.5009961441076598 1102 7 0.5872094302283912 1102 8 0.44404396589324335 1102 9 0.6388696227562534 1102 10-11 0.6091331820823243 1102 12-13 0.561948829983244 1102 14-15 0.8110257882170444 1102 16-17 0.6920260396216023 1102 18-19 0.8311295463321642 1102 20-21 0.9295418509823961 1102 22-23 0.7481956399852336 1102 24-25 0.8461343065705265 1102 26-27 0.7102324774910471 1102 28-29 0.5570656538915557 1102 30-31 0.6432898158693163 1102 32-33 0.7472411748470549 1102 34-35 0.4961374015781672 1102 36-37 0.5382591329742183 1102 38-39 0.33214202763162604 1102 40-41 0.6207516257752772 1102 42-43 0.48387244957589104 1102 44-45 0.6201088837073954 1102 46-47 0.45545155500589374 1102 48-49 0.7209191321875252 1102 50-51 0.7258939897790242 1102 52-53 0.5400074018570749 1102 54-55 0.7573852101588514 1102 56-57 0.6789182225030288 1102 58-59 0.5762056820880517 1102 60-61 0.6622986048661801 1102 62-63 0.7713882167095534 1102 64-65 0.7967615082213797 1102 66-67 0.5989311537289552 1102 68-69 0.7583663042525757 1102 70-71 0.5080228230660069 1102 72-73 0.7413159193950172 1102 74-75 0.5763685671793297 1102 76-77 0.7978433444128612 1102 78-79 0.7785784708438506 1102 80-81 0.9284617383280214 1102 82-83 0.7892164358192275 1102 84-85 0.5930749938363782 1102 86-87 0.5229365648665656 1102 88-89 0.6423040200654064 1102 90-91 1.0305054113070682 1102 92-93 1.0755748948470192 1102 94-95 1.0615179611164436 1102 96-97 1.063438360862829 1102 98-99 1.010053435942016 1102 100-101 1.4439854402067667 1103 1 0.2598652573366813 1103 2 0.30558033406586915 1103 3 0.5244170253449063 1103 4 0.5177300664724314 1103 5 0.49110766356748314 1103 6 0.4814048154880197 1103 7 0.5293409014259041 1103 8 0.3725585689412583 1103 9 0.5843418511920788 1103 10-11 0.42201868212608673 1103 12-13 0.3654223422035088 1103 14-15 0.7191505876257196 1103 16-17 0.7871034070854535 1103 18-19 0.9946442952647772 1103 20-21 0.9278087419871568 1103 22-23 0.5583657414236569 1103 24-25 0.5568675225478117 1103 26-27 0.5503239957606567 1103 28-29 0.6348897365205914 1103 30-31 0.5963806241672316 1103 32-33 0.4987583746408788 1103 34-35 0.4564033005676791 1103 36-37 0.3721525010605049 1103 38-39 0.32830283135849214 1103 40-41 0.4173607814924978 1103 42-43 0.49636114351973504 1103 44-45 0.49878201468258965 1103 46-47 0.7140869504990306 1103 48-49 0.8529344792469757 1103 50-51 0.7155391011862235 1103 52-53 0.8445993626433008 1103 54-55 0.7874481350477467 1103 56-57 0.8253328654261693 1103 58-59 0.6752389535014771 1103 60-61 0.6370416048370018 1103 62-63 0.6837927838699542 1103 64-65 0.596619027599715 1103 66-67 0.6672810044403832 1103 68-69 0.5626198127770934 1103 70-71 0.55356702123564 1103 72-73 0.6160856648750261 1103 74-75 0.42087941613041835 1103 76-77 0.5301713707887998 1103 78-79 0.224006447619022 1103 80-81 0.5614744497391158 1103 82-83 0.5175525733786763 1103 84-85 0.7330420239177826 1103 86-87 0.5399603246043618 1103 88-89 0.3401809129180329 1103 90-91 0.44308017716229386 1103 92-93 0.34856387569723424 1103 94-95 0.4919582408258414 1103 96-97 0.6728941140649951 1103 98-99 0.8372492422461733 1103 100-101 1.1618149691940367 1104 1 0.23127957746919137 1104 2 0.0923236709127302 1104 3 0.26111895276547514 1104 4 0.30081055133916834 1104 5 0.19577939975179248 1104 6 0.20439834055954265 1104 7 0.22203778442545286 1104 8 0.347419282689188 1104 9 0.515831837639908 1104 10-11 0.22673935672329293 1104 12-13 0.35580970856398864 1104 14-15 0.4031994883936747 1104 16-17 0.3971461340844016 1104 18-19 0.2714663100215944 1104 20-21 0.42431999782211705 1104 22-23 0.18575995916194898 1104 24-25 0.3570388069929251 1104 26-27 -0.04098190696483073 1104 28-29 -0.10851110672590636 1104 30-31 0.03637742434792557 1104 32-33 0.014823349795214824 1104 34-35 0.17811219230084419 1104 36-37 0.23582796409318263 1104 38-39 0.05398702801561939 1104 40-41 0.2924003086721356 1104 42-43 0.22884307395190007 1104 44-45 0.21476511210767057 1104 46-47 0.29264627891342343 1104 48-49 0.45534093158848776 1104 50-51 0.16855728368886247 1104 52-53 0.07672837182865777 1104 54-55 0.2076324069947404 1104 56-57 0.14234461064526016 1104 58-59 0.17475898060583006 1104 60-61 0.08066172981817488 1104 62-63 0.2002003279145228 1104 64-65 0.2880500997274069 1104 66-67 0.31396072135048314 1104 68-69 0.05358220548903603 1104 70-71 -0.07466667850837538 1104 72-73 0.06999603228956985 1104 74-75 0.16942161610030126 1104 76-77 0.1702076981491345 1104 78-79 -0.028943032880903274 1104 80-81 0.17968236270403182 1104 82-83 0.05314585752262957 1104 84-85 0.10638282349615835 1104 86-87 0.33039662937773784 1104 88-89 0.25267620730140905 1104 90-91 0.3883331511120005 1104 92-93 0.3540553302974452 1104 94-95 0.27625089253492163 1104 96-97 0.1620401387600694 1104 98-99 0.03367297361193167 1104 100-101 0.5886906279803625 1105 1 0.4283182580025553 1105 2 0.35616361804110497 1105 3 0.48137394216210083 1105 4 0.40715333087610617 1105 5 0.5752473426969118 1105 6 0.43151683691372966 1105 7 0.5205620269377249 1105 8 0.5249996795168883 1105 9 0.5549227071167095 1105 10-11 0.6339249593726777 1105 12-13 0.6473331812421463 1105 14-15 0.8022936623471324 1105 16-17 0.7686272295728784 1105 18-19 0.641121046680226 1105 20-21 0.7594166265296636 1105 22-23 0.628480669152367 1105 24-25 0.42759737248692176 1105 26-27 0.4252089392716911 1105 28-29 0.5496614813692631 1105 30-31 0.4334569569278841 1105 32-33 0.4808805644482206 1105 34-35 0.21795383161177995 1105 36-37 0.2781069272863945 1105 38-39 0.19382285725770032 1105 40-41 0.3974605155105877 1105 42-43 0.22706991356495365 1105 44-45 0.28329178758627904 1105 46-47 0.5576932678452451 1105 48-49 0.6709346116860999 1105 50-51 0.6254827041925921 1105 52-53 0.6980998022676133 1105 54-55 0.573933826104053 1105 56-57 0.38081235023797433 1105 58-59 0.49473436043908237 1105 60-61 0.4513450302277846 1105 62-63 0.6245608572117973 1105 64-65 0.5770892232785201 1105 66-67 0.6878502719416559 1105 68-69 0.6437873372323466 1105 70-71 0.8160328537810848 1105 72-73 0.694140318086859 1105 74-75 0.6225770281794354 1105 76-77 0.7372800407688942 1105 78-79 0.915388155568678 1105 80-81 1.0212379723255047 1105 82-83 0.9012989818871553 1105 84-85 0.8315769161086557 1105 86-87 0.8793765991653792 1105 88-89 0.9865454589645424 1105 90-91 0.977352847245399 1105 92-93 1.215443609936056 1105 94-95 1.1474616382572584 1105 96-97 1.1634554352188324 1105 98-99 1.4909849452587984 1105 100-101 1.8218757440364897 1106 1 0.15493109173741715 1106 2 -0.006527511061793945 1106 3 0.05427450411068335 1106 4 0.21700804747698044 1106 5 0.28727166080638256 1106 6 0.09338309585729831 1106 7 0.1311204789192928 1106 8 0.2356388880175473 1106 9 0.37166665269222676 1106 10-11 0.4809134319848951 1106 12-13 0.4085908465241772 1106 14-15 0.283640615568892 1106 16-17 0.1159810292952912 1106 18-19 0.22927739813771808 1106 20-21 0.08775329860621639 1106 22-23 -0.08822275573588456 1106 24-25 -0.34753934032394795 1106 26-27 -0.4471690553602059 1106 28-29 -0.40787493917991924 1106 30-31 -0.3279609395428267 1106 32-33 0.028859479448058778 1106 34-35 -0.016889010077676403 1106 36-37 -0.22943586690340823 1106 38-39 -0.11524975310776142 1106 40-41 -0.1300896350711227 1106 42-43 -0.10343860325683352 1106 44-45 -0.23670488672638612 1106 46-47 6.6572618635518666E-6 1106 48-49 -0.08866448275843908 1106 50-51 -0.30384430345078783 1106 52-53 -0.13025289292780684 1106 54-55 0.10829754093241917 1106 56-57 0.2057711708224801 1106 58-59 -0.03964733588934877 1106 60-61 -0.08306123488290496 1106 62-63 -0.08841096132674409 1106 64-65 -0.1855713550777338 1106 66-67 -0.10931003971868591 1106 68-69 0.01104879394037539 1106 70-71 -0.22535895705211217 1106 72-73 -0.2327805512065062 1106 74-75 -0.053635085156003015 1106 76-77 -0.3235732722067688 1106 78-79 -0.09742286207028172 1106 80-81 -0.08970307612769446 1106 82-83 0.22045209240181052 1106 84-85 0.2412337649949805 1106 86-87 0.2682179923648853 1106 88-89 0.3098790011918666 1106 90-91 0.4927702922202286 1106 92-93 0.5190943529458814 1106 94-95 0.7166621059899008 1106 96-97 0.5360700379060184 1106 98-99 0.5822257470983949 1106 100-101 1.1026675495998575 1107 1 0.2107312275731239 1107 2 0.30610716417848494 1107 3 0.3183283693206178 1107 4 0.47251354686865454 1107 5 0.3878413889883845 1107 6 0.24619338028163185 1107 7 0.363465501615579 1107 8 0.3903034764304678 1107 9 0.6327521656674691 1107 10-11 0.5297348862087503 1107 12-13 0.44716232395736455 1107 14-15 0.2889983081208882 1107 16-17 0.2696451730661451 1107 18-19 0.5152054540746676 1107 20-21 0.3328817806177611 1107 22-23 0.3088364989267518 1107 24-25 0.36064476103489795 1107 26-27 0.30744593836105594 1107 28-29 0.37736050123560005 1107 30-31 0.2549220511880961 1107 32-33 0.3544938273145064 1107 34-35 0.3175942034629031 1107 36-37 0.32623967143108956 1107 38-39 0.3808974967077816 1107 40-41 0.35311779778059105 1107 42-43 0.0924286686442315 1107 44-45 0.14265297195562 1107 46-47 0.23437333549248507 1107 48-49 0.3816153485707545 1107 50-51 0.08821028491327354 1107 52-53 0.09706547567558488 1107 54-55 0.2752850665237716 1107 56-57 0.4131759270267068 1107 58-59 0.4390293897860431 1107 60-61 0.44553633473289267 1107 62-63 0.34249972425268993 1107 64-65 0.35525997899504347 1107 66-67 0.25948024069899134 1107 68-69 0.4641243977496359 1107 70-71 0.5491452181857248 1107 72-73 0.43818187898224537 1107 74-75 0.4078813885881303 1107 76-77 0.4996143227682879 1107 78-79 0.6545975719199184 1107 80-81 0.607084018502885 1107 82-83 0.5459325257079435 1107 84-85 0.5547442873761099 1107 86-87 0.6927571037648228 1107 88-89 0.44214291335795863 1107 90-91 0.41423722013446707 1107 92-93 0.6113453656059669 1107 94-95 0.5230314813899568 1107 96-97 0.6347036186427566 1107 98-99 0.7600789077216739 1107 100-101 1.2398689431374024 1108 1 0.2540259681146999 1108 2 0.2647986142369163 1108 3 0.3898908773568124 1108 4 0.46398767817367315 1108 5 0.3733805213363226 1108 6 0.458359681688286 1108 7 0.48293445546709535 1108 8 0.5454952879391612 1108 9 0.275392152638112 1108 10-11 0.34102017046865996 1108 12-13 0.33474718619196153 1108 14-15 0.6415966068759573 1108 16-17 0.7653845602865275 1108 18-19 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1.197570901114485 2311 82-83 1.1803316011937746 2311 84-85 1.1657294286693833 2311 86-87 1.3082276087917428 2311 88-89 1.363028222169973 2311 90-91 1.3421972770574797 2311 92-93 1.3629733304918048 2311 94-95 1.3435005726502425 2311 96-97 1.3395948633353107 2311 98-99 1.431218364843044 2311 100-101 1.4874514797644274 2312 1 0.22743780159896332 2312 2 0.2226921207389303 2312 3 0.3672584912546313 2312 4 0.43083670307635913 2312 5 0.26576609949231056 2312 6 0.24035636722533837 2312 7 0.4345302367321935 2312 8 0.3737448208193186 2312 9 0.5285751997496675 2312 10-11 0.3759625562924569 2312 12-13 0.2644889438398721 2312 14-15 0.37631511497460934 2312 16-17 0.49833632371508685 2312 18-19 0.37898422049370595 2312 20-21 0.22918305818734552 2312 22-23 0.28246794094957295 2312 24-25 0.39413939122518826 2312 26-27 0.2695597726213066 2312 28-29 0.1427083557151434 2312 30-31 0.24111502981163824 2312 32-33 0.3639986113125886 2312 34-35 0.34655885662841257 2312 36-37 0.27140014043992977 2312 38-39 0.3236813056401857 2312 40-41 0.5331719277135321 2312 42-43 0.339847814719235 2312 44-45 0.15373864622324618 2312 46-47 0.2804522664780933 2312 48-49 0.21765784902896712 2312 50-51 0.292982129063148 2312 52-53 0.2909874943379194 2312 54-55 -0.037460674422433726 2312 56-57 0.265485838185775 2312 58-59 0.40063879685009596 2312 60-61 0.3945085850819794 2312 62-63 0.2873249831685314 2312 64-65 0.3298852295477346 2312 66-67 0.19792370909466683 2312 68-69 0.28623872832794817 2312 70-71 0.2823710354789277 2312 72-73 0.12934874619528003 2312 74-75 -0.0017167188377626985 2312 76-77 0.19554418247441419 2312 78-79 0.13319834969330913 2312 80-81 -0.04954301957643281 2312 82-83 0.34772253392650043 2312 84-85 -0.04115999436372064 2312 86-87 -0.26093515089871033 2312 88-89 -0.08868117393461716 2312 90-91 -0.10418565692364012 2312 92-93 0.1539036624612251 2312 94-95 0.13176050462428535 2312 96-97 -0.06597516469799913 2312 98-99 0.24512996399984033 2312 100-101 0.0214581223093937 2313 1 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-1.8867346498082327 2315 96-97 -1.5433504070426238 2315 98-99 -1.653859007014649 2315 100-101 -2.3226565609426757 2316 1 -5.396914498569711 2316 2 -5.391082516495921 2316 3 -6.340021675899784 2316 4 -8.053088156685156 2316 5 -8.682095967901905 2316 6 -9.646865666369486 2316 7 -11.776982407205576 2316 8 -11.910740373746329 2316 9 -11.555838077010304 2316 10-11 -11.481309326941158 2316 12-13 -10.356212116080982 2316 14-15 -10.166808203011797 2316 16-17 -9.101826803897161 2316 18-19 -9.708592424347486 2316 20-21 -10.821396159993792 2316 22-23 -10.259045522712498 2316 24-25 -10.435380985857904 2316 26-27 -8.322204276549275 2316 28-29 -6.540829896310704 2316 30-31 -8.745332029990841 2316 32-33 -6.574137340581725 2316 34-35 -5.1971409925274585 2316 36-37 -5.810414412167667 2316 38-39 -4.510099408946431 2316 40-41 -4.089655710021816 2316 42-43 -4.213157638800045 2316 44-45 -4.595961015213284 2316 46-47 -4.89519521046747 2316 48-49 -5.33291839734202 2316 50-51 -5.385094555030712 2316 52-53 -5.287188448215005 2316 54-55 -4.525392029570341 2316 56-57 -5.024555279284186 2316 58-59 -5.420800980779255 2316 60-61 -5.715885370454291 2316 62-63 -5.246232077939062 2316 64-65 -6.0206758110613094 2316 66-67 -5.565364058959517 2316 68-69 -5.7450796929573436 2316 70-71 -5.2823770305537 2316 72-73 -4.407075121105475 2316 74-75 -4.597560939521415 2316 76-77 -5.41206902553769 2316 78-79 -5.515991472946585 2316 80-81 -6.2232517618741845 2316 82-83 -5.639136121582009 2316 84-85 -5.484856079226734 2316 86-87 -6.194608558373755 2316 88-89 -7.549377127295031 2316 90-91 -6.649533374808712 2316 92-93 -5.959128889013623 2316 94-95 -5.381403747570456 2316 96-97 -6.483643764774818 2316 98-99 -8.023407630513006 2316 100-101 -8.545985994658082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5517.0 3 2321.0 4 2503.0 5 2796.0 6 3119.0 7 3622.0 8 4413.0 9 5512.0 10 6782.0 11 8389.0 12 10040.0 13 11647.0 14 12741.0 15 14765.0 16 18170.0 17 21345.0 18 18629.0 19 15378.0 20 16282.0 21 17784.0 22 19826.0 23 22300.0 24 26085.0 25 29683.0 26 34433.0 27 40770.0 28 48204.0 29 57410.0 30 68607.0 31 83546.0 32 102941.0 33 129670.0 34 169851.0 35 227152.0 36 302769.0 37 354034.0 38 219978.0 39 23348.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.22647438597141 17.430312903509463 13.765671786849815 23.577540923669314 2 16.357428312506446 19.38415392636104 38.49037960243493 25.768038158697586 3 20.118434331988 23.239372778142144 28.337886128021566 28.30430676184829 4 13.382977522736756 15.561043480835075 35.11738188034884 35.938597116079336 5 15.163072414078622 36.36089037487204 32.32394206577665 16.15209514527269 6 34.01308683152035 34.078636638039704 16.53950682675561 15.368769703684343 7 31.027184879394287 31.431348229326716 19.07694514892908 18.464521742349916 8 28.197101948198572 32.55486827542042 18.78532798809537 20.462701788285635 9 27.493693544438585 16.437832532194044 19.367054456029738 36.70141946733763 10-11 26.71546046466861 25.117638647945668 25.711072354535975 22.455828532849743 12-13 27.604778825381615 22.392572543012506 26.376762178558483 23.625886453047396 14-15 24.008063467651976 23.937829683536194 24.836664811347337 27.217442037464494 16-17 23.977804096441684 27.38677801019333 25.032928535418797 23.602489357946194 18-19 23.795350683729104 25.60804775311118 27.31224026287254 23.28436130028718 20-21 24.652991785866842 24.70350187065394 27.058118281944793 23.58538806153442 22-23 24.089928332072958 24.597408484057993 27.339336880884158 23.97332630298489 24-25 24.38097172059564 24.9660708206282 27.17112055567068 23.48183690310548 26-27 23.911798542048967 25.244391134984212 27.349140832111747 23.494669490855077 28-29 23.424123613836258 25.603483460845172 27.418495132890193 23.553897792428373 30-31 23.438049535768368 24.98771583423764 27.447763102551338 24.126471527442654 32-33 23.506177681535593 25.186690211263112 27.328667272856773 23.978464834344525 34-35 24.580927295054103 25.07882401097084 26.75479107069763 23.585457623277424 36-37 24.413133163742767 24.72713238631778 26.96785988221156 23.891874567727893 38-39 23.862672734971248 24.96055455565687 27.25705063469992 23.919722074671967 40-41 23.941955028883047 25.00878134373174 27.299284149946274 23.74997947743894 42-43 24.115447884645533 25.168905091732956 26.507774790744527 24.207872232876984 44-45 23.88232467680514 25.257074862540012 26.56259502075629 24.298005439898553 46-47 24.269845307188938 25.498184845191574 26.22808518509954 24.003884662519944 48-49 24.577744593662487 25.46009624266971 25.957495711256268 24.004663452411535 50-51 24.001477100775826 25.70122977657579 26.079684823506344 24.217608299142043 52-53 24.580701372250054 25.729931311191795 25.66104827084839 24.028319045709758 54-55 23.974452888929584 25.387771127630117 25.97837638355906 24.65939959988124 56-57 24.22998986477346 25.80278833193318 25.783294843521396 24.183926959771963 58-59 24.697722440419138 25.154205548046804 25.753106945521942 24.394965066012116 60-61 24.32302812038817 25.287815084271443 26.669114746334703 23.72004204900568 62-63 23.955213595842963 25.641860456512212 26.359473303525675 24.043452644119153 64-65 23.80561491749474 25.731947740117565 25.978254183995446 24.48418315839225 66-67 23.94315359240605 25.880718828054437 25.65998706039208 24.516140519147427 68-69 23.724104601339775 25.57386677642608 26.29360797852292 24.408420643711224 70-71 24.015197533950154 25.665551123339718 26.012284465167003 24.306966877543125 72-73 24.111496388305756 25.48690744936655 26.195716754732047 24.20587940759565 74-75 23.693912018680518 26.305521386413794 25.684232727585567 24.316333867320125 76-77 24.059865877675538 25.886672533602855 25.69403503740461 24.359426551316997 78-79 24.21539058577401 25.861218440839213 25.783742142226597 24.139648831160184 80-81 24.05023948672694 26.357392082970264 25.69341536378117 23.898953066521624 82-83 24.158992837862556 26.03303883620643 25.68421617339342 24.123752152537595 84-85 23.923340532491018 26.00957780871206 25.54224918951667 24.524832469280252 86-87 24.204792977945086 25.819417017754 25.651010715293225 24.324779289007694 88-89 23.85784818357303 26.031553117435795 25.346849359161123 24.763749339830053 90-91 23.979194353532733 25.88882211024273 25.727551294363636 24.4044322418609 92-93 24.272084893905742 25.714224927779682 25.74382531332471 24.269864864989867 94-95 23.930280042154447 25.61206392360582 25.796541661048927 24.66111437319081 96-97 23.869538421691026 25.654845942067695 25.87007725155145 24.605538384689833 98-99 23.848407087935406 25.874271599341764 25.388019710039007 24.889301602683823 100-101 23.678667861582493 25.540608828886125 25.760666069208753 25.02005724032263 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 367.0 1 257.0 2 235.5 3 326.0 4 307.0 5 321.0 6 498.0 7 938.0 8 1555.0 9 1846.0 10 1705.0 11 1562.0 12 1617.0 13 1812.0 14 1968.5 15 2107.0 16 2299.5 17 2395.5 18 2453.0 19 2592.0 20 2767.0 21 3069.5 22 3426.5 23 4003.5 24 4694.0 25 5639.0 26 6900.0 27 8268.5 28 9836.0 29 11804.0 30 14104.5 31 16997.5 32 21066.0 33 23766.5 34 25854.5 35 28028.5 36 31174.5 37 35533.5 38 38691.0 39 40031.0 40 41942.5 41 48519.0 42 55758.0 43 61269.5 44 64933.0 45 69334.5 46 72631.5 47 78685.0 48 94981.0 49 98603.5 50 89313.5 51 85007.5 52 84464.5 53 87927.0 54 90102.5 55 86314.5 56 80511.5 57 75050.0 58 70493.0 59 64266.5 60 55794.0 61 47533.5 62 40423.0 63 32640.5 64 25754.5 65 21562.0 66 17140.5 67 14016.5 68 10615.0 69 7142.0 70 5563.5 71 4503.0 72 3843.0 73 3445.5 74 2790.0 75 2313.0 76 1651.0 77 965.5 78 601.5 79 429.0 80 290.5 81 190.5 82 133.0 83 84.0 84 53.0 85 29.5 86 18.0 87 14.5 88 11.0 89 13.0 90 15.0 91 11.5 92 8.0 93 6.0 94 5.0 95 2.5 96 2.0 97 3.0 98 3.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07556544596561911 2 0.030290922342399336 3 0.014891109914889445 4 0.020625574602610844 5 0.024648949020608928 6 0.030013448244606365 7 0.07417807547665425 8 0.054939871363008264 9 0.06858234783782934 10-11 0.05998065080624723 12-13 0.04025686702146355 14-15 0.04675438547811562 16-17 0.05755275245055874 18-19 0.05385309781331912 20-21 0.019400064004025223 22-23 0.014289916036338009 24-25 0.02349280694647155 26-27 0.02603631950957378 28-29 0.0067749925544450425 30-31 0.0012717562815511172 32-33 0.010451524350201908 34-35 0.012440088717718203 36-37 0.004508954089135779 38-39 0.009272259434581782 40-41 0.005063902284721721 42-43 0.0025435125631022345 44-45 3.468426222412138E-4 46-47 6.474395615169323E-4 48-49 8.092994518961654E-4 50-51 9.249136593099034E-4 52-53 1.3873704889648552E-4 54-55 2.7747409779297103E-4 56-57 0.00478642818692875 58-59 0.0033065663320329046 60-61 0.005780710370686897 62-63 0.0025897582460677293 64-65 7.86176610413418E-4 66-67 5.087025126204469E-4 68-69 0.002728495294964215 70-71 0.0018267044771370592 72-73 0.01981627515071468 74-75 0.015052969805268678 76-77 0.024579580496160686 78-79 0.019076344223266758 80-81 0.011098963911718841 82-83 0.004185234308377313 84-85 0.0034221805394466427 86-87 0.001572353220826836 88-89 0.0019423186845507973 90-91 0.0075149234818929645 92-93 0.010636507082063889 94-95 0.014544267292648233 96-97 0.01255570292513194 98-99 0.024117123666505733 100-101 0.01972378378478369 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2162364.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.69287237682089 #Duplication Level Percentage of deduplicated Percentage of total 1 74.64387163125899 33.3604902852766 2 13.20438359564465 11.802836617094673 3 4.84684758284338 6.498586213497661 4 2.1971910556245264 3.927951177460773 5 1.228348013985957 2.7449200511697884 6 0.8117749000026757 2.176833120271567 7 0.4858744450365044 1.5200587194222615 8 0.38528438135383114 1.377557254770333 9 0.27957509754041865 1.1245512738700063 >10 1.6110135702711608 13.583553111629293 >50 0.16705303327454002 5.260348531205319 >100 0.12653905411576635 10.905067071179515 >500 0.00889495144479553 2.596830891975059 >1k 0.003034748139989063 2.292033922682475 >5k 3.1393946275748934E-4 0.8283817584947454 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6375 0.29481622890503173 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6297 0.2912090656337231 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5040 0.23307824214609563 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3672 0.16981414784929827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3028 0.14003192801951939 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2722 0.12588074903207785 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2428 0.11228451824022229 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2426 0.11219202687429129 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2359 0.10909356611560311 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2237 0.10345159279381269 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.3122841482747584E-5 0.0 20-21 0.0 0.0 0.0 6.936852444824274E-5 0.0 22-23 0.0 0.0 0.0 2.081055733447283E-4 0.0 24-25 0.0 0.0 0.0 3.6996546372396134E-4 0.0 26-27 0.0 0.0 0.0 5.780710370686896E-4 0.0 28-29 0.0 0.0 0.0 0.0010867735496891365 0.0 30-31 0.0 0.0 0.0 0.0024047755142057488 0.0 32-33 0.0 0.0 0.0 0.004971410918790731 0.0 34-35 0.0 0.0 0.0 0.007329940750030985 0.0 36-37 0.0 0.0 0.0 0.009017908178271559 0.0 38-39 0.0 0.0 0.0 0.010382155825753666 0.0 40-41 0.0 0.0 0.0 0.012648194291062929 0.0 42-43 0.0 0.0 0.0 0.015538549476406378 0.0 44-45 0.0 0.0 0.0 0.018613887393611805 0.0 46-47 0.0 0.0 0.0 0.022799121701989118 0.0 48-49 0.0 0.0 0.0 0.02834860365784854 0.0 50-51 0.0 0.0 0.0 0.03651096670125844 0.0 52-53 0.0 0.0 0.0 0.054662397265215296 0.0 54-55 2.3122841482747584E-5 0.0 0.0 0.07614351700268779 0.0 56-57 4.624568296549517E-5 0.0 0.0 0.10143990558481367 0.0 58-59 4.624568296549517E-5 0.0 0.0 0.13177707361017849 0.0 60-61 4.624568296549517E-5 0.0 0.0 0.17626542062298484 0.0 62-63 4.624568296549517E-5 0.0 0.0 0.21125027978638194 0.0 64-65 4.624568296549517E-5 0.0 0.0 0.24415408321633175 0.0 66-67 4.624568296549517E-5 0.0 0.0 0.30684010647606047 0.0 68-69 4.624568296549517E-5 0.0 0.0 0.3861283299204019 0.0 70-71 4.624568296549517E-5 4.624568296549517E-5 0.0 0.45290709612257696 0.0 72-73 4.624568296549517E-5 4.624568296549517E-5 0.0 0.5120090789524798 0.0 74-75 4.624568296549517E-5 4.624568296549517E-5 0.0 0.6197383974206008 0.0 76-77 4.624568296549517E-5 4.624568296549517E-5 0.0 0.7040211546252158 0.0 78-79 4.624568296549517E-5 4.624568296549517E-5 0.0 0.7807196198234895 0.0 80-81 4.624568296549517E-5 4.624568296549517E-5 0.0 0.876586920610961 0.0 82-83 4.624568296549517E-5 4.624568296549517E-5 0.0 1.0251974228205798 0.0 84-85 4.624568296549517E-5 4.624568296549517E-5 0.0 1.181715936817298 0.0 86-87 4.624568296549517E-5 4.624568296549517E-5 0.0 1.3798093197999965 0.0 88-89 4.624568296549517E-5 4.624568296549517E-5 0.0 1.6191076063049514 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10765 0.0 27.589983 1 TACATGG 11230 0.0 25.85514 2 ACATGGG 11150 0.0 24.763775 3 GTAAGGT 685 0.0 22.895443 4 GTATCAA 16015 0.0 22.640347 1 GAGTACT 6930 0.0 22.56125 12-13 GTACTTT 7155 0.0 21.516697 14-15 TAAGGTG 715 0.0 21.27158 5 AGTACTT 7125 0.0 21.210104 12-13 CGTATAG 115 0.0037148038 20.661299 1 CATGGGG 5530 0.0 20.368023 4 AGAGTAC 12970 0.0 20.265673 10-11 GTACTAG 235 1.12775524E-7 20.221697 1 CGCGGAA 715 0.0 19.925505 82-83 CATGGGA 5650 0.0 19.767199 4 TCAACGC 18720 0.0 19.319883 4 TATCAAC 18830 0.0 19.306189 2 ATTCGCG 665 0.0 19.282413 80-81 GGTATCA 12250 0.0 19.202358 1 ATCAACG 18855 0.0 19.1307 3 >>END_MODULE