FastQCFastQC Report
Thu 26 May 2016
SRR936464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162364
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73500.3399057697963895No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68650.31747661355812434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64190.29685103895551346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39350.18197676246922348No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33500.1549230379344088No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26520.12264355122449319No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG25280.11690908653677179No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG24800.11468929375442803No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24440.11302444916767021No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG23110.10687377333325934No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG22360.1034053471108472No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC22070.10206422230484784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG105000.025.0677681
GTAAGGT5650.024.3777794
TACATGG109800.023.5359362
TAAGGTG6800.023.0488685
GTACTAG2303.621608E-922.7227081
ACATGGG110250.022.402653
GAGTACT76850.022.093112-13
GTACTTT79450.021.30983414-15
GATGTGC14350.021.1812958
GTATCAA166750.020.8279611
AGTACTT80700.020.71541412-13
GGTATCA131850.020.6836411
TACTTTT84650.019.55195614-15
CATGGGG57350.019.2131754
AGAGTAC141550.019.07414610-11
ACTTTTT90100.018.84367816-17
CATGGGA54350.018.3511924
ATGGGAG24400.018.2971155
ATGGGGA31700.018.2786925
CGTATAG1300.007555026618.2735061
Produced by FastQC (version 0.11.5)