##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936464_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2162364 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.769508278902165 31.0 31.0 34.0 30.0 34.0 2 31.93426129920772 31.0 31.0 34.0 30.0 34.0 3 32.01356617109793 31.0 31.0 34.0 30.0 34.0 4 35.73323131535671 37.0 35.0 37.0 35.0 37.0 5 35.546472286812026 37.0 35.0 37.0 33.0 37.0 6 35.631678107848636 37.0 35.0 37.0 33.0 37.0 7 35.56579650789599 37.0 35.0 37.0 33.0 37.0 8 35.52900159270132 37.0 35.0 37.0 33.0 37.0 9 37.139409923583635 39.0 37.0 39.0 33.0 39.0 10-11 37.0159080524833 39.0 37.0 39.0 33.0 39.0 12-13 36.896067452103345 39.0 37.0 39.0 33.0 39.0 14-15 38.00127060013948 40.0 37.0 41.0 33.0 41.0 16-17 37.884799691448805 40.0 37.0 41.0 33.0 41.0 18-19 38.01377705141225 40.0 37.0 41.0 33.0 41.0 20-21 38.01844902153384 40.0 37.0 41.0 33.0 41.0 22-23 37.92910305572975 40.0 37.0 41.0 33.0 41.0 24-25 37.770490537208346 40.0 37.0 41.0 32.0 41.0 26-27 37.56004077019411 39.0 36.5 41.0 32.0 41.0 28-29 37.46695884689164 39.0 36.5 41.0 32.0 41.0 30-31 37.45032797438359 39.0 36.0 41.0 32.0 41.0 32-33 37.30168371282541 39.0 36.0 41.0 31.5 41.0 34-35 37.11208427443298 39.0 36.0 41.0 31.0 41.0 36-37 36.90011256199234 39.0 35.0 41.0 31.0 41.0 38-39 36.724140338999355 39.0 35.0 40.5 30.0 41.0 40-41 36.515192631767825 39.0 35.0 40.0 30.0 41.0 42-43 36.2115920353835 38.5 35.0 40.0 30.0 41.0 44-45 35.93041180855767 38.0 35.0 40.0 29.5 41.0 46-47 35.54057665591917 38.0 34.5 40.0 27.5 41.0 48-49 35.138186956497606 38.0 34.0 40.0 27.0 41.0 50-51 34.79980058861506 38.0 34.0 40.0 26.0 41.0 52-53 34.40893554461691 38.0 33.0 40.0 24.0 41.0 54-55 34.1707716647151 37.0 33.0 40.0 24.5 41.0 56-57 33.82718311995575 37.0 33.0 40.0 23.5 41.0 58-59 33.40325195018045 36.0 32.0 39.0 23.0 41.0 60-61 32.94243522367187 36.0 32.0 39.0 22.0 40.0 62-63 32.59343824628971 35.0 31.0 39.0 21.5 40.0 64-65 32.14074411153719 35.0 31.0 38.0 20.0 40.0 66-67 31.684533223823557 35.0 30.5 38.0 19.5 40.0 68-69 31.31110696441487 34.5 30.0 37.0 18.0 39.0 70-71 30.91727895950913 34.0 30.0 36.5 17.5 39.0 72-73 30.45278130786491 34.0 29.0 36.0 14.0 39.0 74-75 29.95580161341939 34.0 29.0 35.5 11.0 38.0 76-77 28.498788363106303 32.0 27.0 34.5 8.0 36.0 78-79 29.280969115283085 33.5 29.0 35.0 7.0 37.0 80-81 29.07923781564991 34.0 29.0 35.0 7.0 36.0 82-83 28.792306706918907 33.0 29.0 35.0 2.0 36.0 84-85 28.4234446651905 33.0 27.5 35.0 2.0 35.5 86-87 28.026646068839476 33.0 27.0 35.0 2.0 35.0 88-89 27.552889337780318 33.0 26.5 35.0 2.0 35.0 90-91 27.06296858438265 32.5 25.5 34.0 2.0 35.0 92-93 26.41072317149194 32.0 24.5 34.0 2.0 35.0 94-95 25.81374504939964 32.0 23.5 34.0 2.0 35.0 96-97 25.013365696062273 31.5 19.5 34.0 2.0 35.0 98-99 24.04055307062086 31.0 9.5 34.0 2.0 35.0 100-101 20.75473185828103 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28944545712731085 1101 2 0.3215526070148016 1101 3 0.28574377244771654 1101 4 0.268859197058255 1101 5 0.2134641617532651 1101 6 0.169749958262706 1101 7 0.23977988854597498 1101 8 0.2640586657001833 1101 9 0.3737863706652149 1101 10-11 0.3927271746124177 1101 12-13 0.36534990176689774 1101 14-15 0.5956547951192164 1101 16-17 0.5533616265311849 1101 18-19 0.42066711523045797 1101 20-21 0.43164473551118476 1101 22-23 0.44986843592612047 1101 24-25 0.4563622806165668 1101 26-27 0.46613362768774635 1101 28-29 0.29136131626283657 1101 30-31 0.45643454780580583 1101 32-33 0.320992713286401 1101 34-35 0.3471102679047817 1101 36-37 0.4143584225251473 1101 38-39 0.35401591426069245 1101 40-41 0.42378612668095883 1101 42-43 0.4239615877871543 1101 44-45 0.3999583900785808 1101 46-47 0.3797597389051859 1101 48-49 0.15306528589430712 1101 50-51 0.1487293472387492 1101 52-53 0.10968187812228081 1101 54-55 0.15325545287877418 1101 56-57 0.09618569959167189 1101 58-59 -0.0384115363716262 1101 60-61 0.06981764732996254 1101 62-63 0.01254286102939517 1101 64-65 0.03718383928594449 1101 66-67 -0.00780541454185979 1101 68-69 -0.061111414732408775 1101 70-71 -0.22700899812400266 1101 72-73 0.08255035029374014 1101 74-75 0.13506490582316388 1101 76-77 -0.009993664174444916 1101 78-79 0.09013524850899657 1101 80-81 0.10793044011380815 1101 82-83 0.03829260194332207 1101 84-85 0.07929522366613995 1101 86-87 -0.040271639824752015 1101 88-89 0.03253860236374706 1101 90-91 0.1631437673829268 1101 92-93 0.07700190912910898 1101 94-95 0.30662223021828083 1101 96-97 0.421287110984931 1101 98-99 0.2703989027006308 1101 100-101 0.7517625387743436 1102 1 0.33004430974719057 1102 2 0.34626952822505075 1102 3 0.3251966700100368 1102 4 0.2520764216035545 1102 5 0.20483377043068174 1102 6 0.18960150730730163 1102 7 0.26645667995324374 1102 8 0.22859513308861068 1102 9 0.31313992090686327 1102 10-11 0.3947534120769447 1102 12-13 0.39181374789256296 1102 14-15 0.5272682118934355 1102 16-17 0.6156755029903493 1102 18-19 0.4533502461368357 1102 20-21 0.28138649355160084 1102 22-23 0.3033414187479906 1102 24-25 0.36842402253339657 1102 26-27 0.4024722514016261 1102 28-29 0.3905956497196783 1102 30-31 0.463478842867346 1102 32-33 0.47929116648118963 1102 34-35 0.44404129073431164 1102 36-37 0.35211396704211495 1102 38-39 0.41863432283061996 1102 40-41 0.5179646383440897 1102 42-43 0.5656745351045771 1102 44-45 0.5432899047587725 1102 46-47 0.5014092097992702 1102 48-49 0.6439475226748002 1102 50-51 0.6931642686362878 1102 52-53 0.7348475927104587 1102 54-55 0.5262850982821305 1102 56-57 0.37656999864339724 1102 58-59 0.5588626215173207 1102 60-61 0.7588197157086043 1102 62-63 0.6896994918523092 1102 64-65 0.542189987419313 1102 66-67 0.42355322114255856 1102 68-69 0.5438046489964492 1102 70-71 0.590355508824203 1102 72-73 0.7213794769737909 1102 74-75 0.7643908912549371 1102 76-77 0.6533579642665757 1102 78-79 0.6491310669639851 1102 80-81 0.6450297426467593 1102 82-83 0.579502705240106 1102 84-85 0.4691559949107891 1102 86-87 0.4912327595514796 1102 88-89 0.5140200989252683 1102 90-91 0.40421808243608126 1102 92-93 0.3512970113876328 1102 94-95 0.25642078723916484 1102 96-97 0.7084792462601541 1102 98-99 0.766320165877385 1102 100-101 1.693198616509548 1103 1 0.1833758276277777 1103 2 0.21894728860473833 1103 3 0.23107679177504536 1103 4 0.16177969233618938 1103 5 0.240268166128061 1103 6 0.19014225287483555 1103 7 0.1286385299644337 1103 8 0.07557483567855172 1103 9 0.2697839916706144 1103 10-11 0.2372379239957212 1103 12-13 0.3211987956940803 1103 14-15 0.3843022808093224 1103 16-17 0.32802074374856005 1103 18-19 0.3717749842739906 1103 20-21 0.26451737148509835 1103 22-23 0.1826676264292999 1103 24-25 0.20892888049042568 1103 26-27 0.16939097423062321 1103 28-29 0.1899031841727563 1103 30-31 0.2688843534148049 1103 32-33 0.2095797262112029 1103 34-35 0.31753649113111493 1103 36-37 0.3600233435313527 1103 38-39 0.24693301667721101 1103 40-41 0.35446058761515786 1103 42-43 0.2617103643612424 1103 44-45 0.230836223117862 1103 46-47 0.13056424622178753 1103 48-49 0.1060885249992296 1103 50-51 0.26743119854359776 1103 52-53 0.2778937603221294 1103 54-55 0.2095035069729576 1103 56-57 0.07102443888420851 1103 58-59 0.1657814416027108 1103 60-61 0.14831748367429753 1103 62-63 0.267412215906738 1103 64-65 0.32994686587799293 1103 66-67 0.024212619514486278 1103 68-69 0.15526971936145628 1103 70-71 -0.030251176580531336 1103 72-73 0.008586370507213559 1103 74-75 -0.043251516424426484 1103 76-77 0.09926006597731529 1103 78-79 -0.04077371902331706 1103 80-81 0.04951374883226478 1103 82-83 0.16249547908980588 1103 84-85 0.5451093507427736 1103 86-87 0.4874514308345823 1103 88-89 0.418782744688297 1103 90-91 0.3853731966637177 1103 92-93 0.09058373297174427 1103 94-95 0.15403946435765192 1103 96-97 0.2794088229030578 1103 98-99 0.4666795282644465 1103 100-101 0.7577569265823954 1104 1 0.139045154536376 1104 2 0.15146498172324385 1104 3 0.13343336458034827 1104 4 0.11214861267951193 1104 5 0.03137455070869066 1104 6 0.033881900518267116 1104 7 0.05797710849176241 1104 8 0.08447409783786242 1104 9 0.07141213502251986 1104 10-11 0.14783497263297107 1104 12-13 0.1122101268189084 1104 14-15 0.20356707526799056 1104 16-17 0.27836990050206367 1104 18-19 0.07899151792855719 1104 20-21 0.20133599819793346 1104 22-23 0.0885835276489999 1104 24-25 0.1773005489138626 1104 26-27 0.1513543457108213 1104 28-29 0.29175531487596373 1104 30-31 0.11669624168463599 1104 32-33 0.12394691488761111 1104 34-35 -0.03526617413014321 1104 36-37 0.017352811984899574 1104 38-39 0.19859409934548466 1104 40-41 0.2527891525903172 1104 42-43 0.14823347835010026 1104 44-45 0.16777343136963196 1104 46-47 0.07372604414378969 1104 48-49 0.17225420034933592 1104 50-51 0.08085914614185441 1104 52-53 0.23648245178425498 1104 54-55 0.16405101487840312 1104 56-57 0.14079461656828585 1104 58-59 0.10264430111182321 1104 60-61 0.20188716444526023 1104 62-63 0.27228723472919825 1104 64-65 0.2632842774123958 1104 66-67 0.33972421415385057 1104 68-69 0.24757980067451157 1104 70-71 0.10652698182935083 1104 72-73 0.2172155302723091 1104 74-75 0.13816882689735976 1104 76-77 0.020323347109677314 1104 78-79 0.009057819901546083 1104 80-81 0.23437483902952394 1104 82-83 0.11186869095548602 1104 84-85 0.4975562713872499 1104 86-87 0.5606585909008359 1104 88-89 0.44304772737162423 1104 90-91 0.5309028608709205 1104 92-93 0.4704540461775899 1104 94-95 0.5019285247401228 1104 96-97 0.6132412653063071 1104 98-99 0.5308481399193177 1104 100-101 1.0804389398334067 1105 1 -0.006502880207833073 1105 2 0.11542367213372984 1105 3 0.07939536673007552 1105 4 0.13776811127056732 1105 5 0.19849355544390335 1105 6 0.16079631820411322 1105 7 0.15786490153710986 1105 8 0.15628654881540882 1105 9 0.19976563413648307 1105 10-11 0.2798825861471954 1105 12-13 0.2998043588730539 1105 14-15 0.2264859812320239 1105 16-17 0.2160443841321964 1105 18-19 0.2060540923700671 1105 20-21 0.2651381798787895 1105 22-23 0.25504744712144145 1105 24-25 0.1517436901275815 1105 26-27 0.14636404126321878 1105 28-29 0.23005964628229236 1105 30-31 0.18930812182291845 1105 32-33 0.16582625428277709 1105 34-35 0.20616070615339055 1105 36-37 0.20278333808663973 1105 38-39 0.1890098460845806 1105 40-41 0.37832004763141214 1105 42-43 0.47550739404599796 1105 44-45 0.4415119201707256 1105 46-47 0.252501499507936 1105 48-49 0.15617792140792375 1105 50-51 0.2323966356111029 1105 52-53 0.18307157824040843 1105 54-55 0.06478598858783613 1105 56-57 0.007387991637287428 1105 58-59 -0.08191816439089195 1105 60-61 0.17185945767943878 1105 62-63 0.08420586471496705 1105 64-65 0.036669622966243764 1105 66-67 -0.22150628580257958 1105 68-69 -0.11442434626213327 1105 70-71 -0.061333351378099366 1105 72-73 -0.006259871348699875 1105 74-75 -0.04552339923051818 1105 76-77 -0.13973442676244474 1105 78-79 0.02376110694799749 1105 80-81 0.05766776817573671 1105 82-83 0.2685731472782926 1105 84-85 0.4165058109392952 1105 86-87 0.46321120833685114 1105 88-89 0.4927192769169899 1105 90-91 0.4763423199535062 1105 92-93 0.4078699817439251 1105 94-95 0.6491478621017706 1105 96-97 0.7017658346349549 1105 98-99 0.5424753586393578 1105 100-101 1.0908413234141534 1106 1 0.12670656474420738 1106 2 0.009078588275844623 1106 3 -0.07749589183962513 1106 4 0.10228473650263226 1106 5 0.06787985574847966 1106 6 -0.01658957760573543 1106 7 -0.08523892613673922 1106 8 0.010699066503150334 1106 9 0.011557367773853855 1106 10-11 0.029594526076877514 1106 12-13 0.0768640239827576 1106 14-15 0.06006684207942925 1106 16-17 0.021308477177377938 1106 18-19 0.1309524879929853 1106 20-21 0.021179251761353157 1106 22-23 -0.043577911707401995 1106 24-25 0.08935002132474068 1106 26-27 -0.014396266409150371 1106 28-29 -0.1809397619305244 1106 30-31 -0.0075992738078269895 1106 32-33 -0.03564114294314891 1106 34-35 0.05593933786449412 1106 36-37 -0.0381263905102216 1106 38-39 0.2627386252439905 1106 40-41 0.12397724831421186 1106 42-43 0.23227609355982537 1106 44-45 0.2625602095781616 1106 46-47 0.25542037766172854 1106 48-49 0.1968652491148859 1106 50-51 0.3315987911122633 1106 52-53 0.08088556193670371 1106 54-55 0.12983059339007497 1106 56-57 -0.04582710057398742 1106 58-59 0.10111053978031492 1106 60-61 0.16844154839022707 1106 62-63 0.2411733221166159 1106 64-65 0.16105495832300676 1106 66-67 0.147106453929986 1106 68-69 -0.00811273291921566 1106 70-71 0.24730200587692153 1106 72-73 0.4512532266607998 1106 74-75 0.22865655534927853 1106 76-77 0.09390472263070748 1106 78-79 -0.06526939466107962 1106 80-81 0.18363191484495545 1106 82-83 0.2700255539863541 1106 84-85 0.4239124086187225 1106 86-87 0.556074796048474 1106 88-89 0.37991830043251795 1106 90-91 0.6152519004255304 1106 92-93 0.8786329528147157 1106 94-95 0.7790718555472473 1106 96-97 0.7952349182051321 1106 98-99 0.7085252539193725 1106 100-101 0.9889811105274369 1107 1 0.0017377141195069612 1107 2 0.0640920496921531 1107 3 0.014254287727361259 1107 4 0.03608437394834141 1107 5 0.044155025665268965 1107 6 -0.030710627742791985 1107 7 0.0010118617592240753 1107 8 0.03975471516751128 1107 9 -0.050521504887498736 1107 10-11 0.09659988621251614 1107 12-13 -0.07783354612166704 1107 14-15 -0.018978056996594717 1107 16-17 0.16504146717537793 1107 18-19 0.009168202913897971 1107 20-21 -0.021302388430385122 1107 22-23 -0.04603199386357204 1107 24-25 -0.05273999181907385 1107 26-27 0.031233293004312657 1107 28-29 0.0343732709681035 1107 30-31 -0.02361616043387471 1107 32-33 0.019885367007546506 1107 34-35 0.07694623225956576 1107 36-37 0.02625753592350577 1107 38-39 -0.05527328725435865 1107 40-41 -4.370212756867886E-4 1107 42-43 -0.02349692399620551 1107 44-45 0.06347661198412169 1107 46-47 0.069621072476032 1107 48-49 0.12352007494713035 1107 50-51 0.09190373738875479 1107 52-53 0.11809746075375926 1107 54-55 0.150304894544675 1107 56-57 -0.022157239976607457 1107 58-59 0.04740657367083401 1107 60-61 0.06699597306737815 1107 62-63 0.07251716724436363 1107 64-65 -0.09886886618508584 1107 66-67 0.07129864679714615 1107 68-69 0.11195894729030442 1107 70-71 0.16972051666803978 1107 72-73 0.29251967475250495 1107 74-75 0.2712566853215783 1107 76-77 0.1396869750603571 1107 78-79 0.13849149540483552 1107 80-81 0.3994567747338458 1107 82-83 0.33623691043782244 1107 84-85 0.5262729204945735 1107 86-87 0.6387721120717131 1107 88-89 0.3530919698044954 1107 90-91 0.33597881690610976 1107 92-93 0.4561973404057831 1107 94-95 0.47330103677064983 1107 96-97 0.7794342448900125 1107 98-99 0.6016230409700292 1107 100-101 0.8744943300780079 1108 1 0.07128474408416352 1108 2 0.06784727500566845 1108 3 -0.013959547959920826 1108 4 0.06882740995907 1108 5 0.06278935801092445 1108 6 0.09802102472778529 1108 7 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-1.6132205277770488 2316 80-81 -1.360605898623625 2316 82-83 -1.5983780204611726 2316 84-85 -1.6135428101551774 2316 86-87 -1.7479243183902007 2316 88-89 -1.9489167838970722 2316 90-91 -1.945288291982795 2316 92-93 -1.825217226310187 2316 94-95 -1.7884320690995352 2316 96-97 -2.2436190849345827 2316 98-99 -3.018409898839952 2316 100-101 -3.775399197808916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 18.0 4 44.0 5 100.0 6 235.0 7 553.0 8 1163.0 9 2041.0 10 3189.0 11 4737.0 12 6608.0 13 8772.0 14 11227.0 15 14542.0 16 18095.0 17 22759.0 18 20295.0 19 15460.0 20 15694.0 21 16133.0 22 17508.0 23 19498.0 24 23283.0 25 27816.0 26 34169.0 27 41912.0 28 51541.0 29 62947.0 30 76230.0 31 92473.0 32 113358.0 33 142325.0 34 183245.0 35 243618.0 36 325724.0 37 369885.0 38 169143.0 39 6019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.500946155408165 18.444595442819693 14.544288907894742 23.5101694938774 2 16.80405643225073 20.13734813932955 37.310351940731614 25.74824348768811 3 20.12619775946483 23.322859249214446 28.81985716148929 27.731085829831436 4 13.871855062329933 16.271034848896857 34.757977842768376 35.099132246004835 5 14.981267519066618 36.566330001521536 32.543228535342436 15.909173944069405 6 34.18402267148362 34.43097461851936 16.08165877715315 15.303343932843868 7 30.80286205282737 31.68356483922226 18.94852115555013 18.565051952400243 8 28.357159109197156 32.71169886291115 18.50040048761448 20.430741540277214 9 27.15282903340973 16.195700631346064 19.29258903681341 37.35888129843079 10-11 26.742676071188754 25.31558054055654 25.494296982376696 22.44744640587801 12-13 27.547296420809523 22.454703721165874 26.446316536225805 23.551683321798798 14-15 23.891309470048803 24.0256765815305 24.955956676029125 27.127057272391568 16-17 23.877137215828885 27.448761505639546 25.271051791246013 23.403049487285557 18-19 23.641245974556725 25.483232397132827 27.731029285641778 23.14449234266867 20-21 24.40738469563866 24.463573200441736 27.672121807429278 23.45692029649032 22-23 23.93863193613467 24.42342576695628 27.8859811895554 23.751961107353647 24-25 24.271260527829728 24.782853395635517 27.77483346929564 23.171052607239115 26-27 23.86452397898379 25.085659873534627 27.78383605470208 23.265980092779508 28-29 23.279099365582358 25.429704878790986 27.956946729821695 23.334249025804954 30-31 23.394757616412242 24.815234628089886 27.91845119424031 23.871556561257563 32-33 23.475944002806383 24.948160810058017 27.93035661801518 23.645538569120422 34-35 24.474748308587927 24.811539269964836 27.289640920483343 23.424071500963894 36-37 24.397439087216988 24.45852991693312 27.548148915487474 23.595882080362422 38-39 23.801131227944904 24.70848138856947 27.793240703765203 23.697146679720422 40-41 23.81947766755605 24.801322259930533 27.836364784100326 23.54283528841309 42-43 23.99717611453524 24.981651219159133 27.019001798573626 24.002170867731998 44-45 23.923207658423227 24.951938307146243 26.970783762847862 24.154070271582672 46-47 24.23381202306183 25.405609886600804 26.601108695541587 23.759469394795783 48-49 24.76262003111421 25.29634343281125 26.2279661628335 23.713070373241038 50-51 24.088503158696494 25.50244211946789 26.371213720542357 24.037841001293263 52-53 24.64970002696127 25.620930085217037 25.860806775556906 23.868563112264788 54-55 24.0365918995494 25.229329477627555 26.234677165186827 24.499401457636218 56-57 24.305784244801302 25.651201620661585 26.022233219862358 24.02078091467476 58-59 24.860801253748548 25.05142617135335 25.971060722894464 24.116711852003643 60-61 24.421184140110093 25.209382055239764 26.86929018477241 23.50014361987773 62-63 24.04974038050392 25.422360091882616 26.60534992147704 23.92254960613642 64-65 23.909631294404583 25.510503075957807 26.274237000346208 24.305628629291405 66-67 24.026964575292677 25.718320404695238 25.946154555862144 24.308560464149938 68-69 23.87977356148603 25.337051813464313 26.456133665502346 24.327040959547308 70-71 24.125683887731075 25.494257088835514 26.258491006244792 24.121568017188615 72-73 24.208204539106273 25.312759553895642 26.394029867311886 24.085006039686196 74-75 23.922123856153384 26.10703449194411 25.754683256171234 24.216158395731274 76-77 24.157975765832887 25.76197129100748 25.9017517766805 24.178301166479134 78-79 24.21888730406277 25.62090342488942 26.070867627552296 24.089341643495523 80-81 24.136001983310223 26.170791316727428 25.82486834205811 23.86833835790424 82-83 24.242144622025123 25.867289972072438 25.812734560517885 24.07783084538455 84-85 23.993664440632234 25.828969039903242 25.6948194913743 24.48254702809022 86-87 24.198765347877515 25.625379397104037 25.75377026775463 24.42208498726382 88-89 23.92409443689834 25.92672868452317 25.350839776306387 24.798337102272097 90-91 24.03708889810616 25.782229724556032 25.818618151231966 24.36206322610584 92-93 24.253074229759903 25.569061519751724 25.857923239433035 24.319941011055338 94-95 23.943539808639862 25.4296605667067 25.91151877738974 24.715280847263692 96-97 23.91245507408381 25.460201467980916 26.057563470001888 24.56977998793339 98-99 23.85897871003548 25.625712397280033 25.45740908208644 25.057899810598045 100-101 23.520880053434723 25.523062696264915 25.77922361862932 25.17683363167104 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 452.0 1 344.5 2 313.5 3 407.0 4 337.5 5 301.0 6 533.5 7 1037.0 8 1665.5 9 2020.5 10 1886.0 11 1661.0 12 1653.0 13 1819.5 14 2062.5 15 2323.5 16 2496.5 17 2648.5 18 2849.0 19 2991.0 20 3217.5 21 3542.0 22 3903.5 23 4385.5 24 5033.0 25 5935.5 26 7027.5 27 8402.5 28 10013.5 29 11982.5 30 14391.5 31 17427.5 32 21599.0 33 24051.5 34 25549.5 35 27755.5 36 31223.5 37 35386.5 38 38449.5 39 39887.0 40 41852.0 41 49013.5 42 56555.0 43 61360.5 44 64284.0 45 68251.0 46 71738.0 47 78993.5 48 96239.0 49 99219.5 50 89228.0 51 84868.0 52 84201.0 53 87817.0 54 90310.0 55 86424.5 56 80108.5 57 74464.0 58 70374.0 59 64449.0 60 55587.0 61 46859.5 62 39600.0 63 32104.5 64 25121.5 65 20810.0 66 16625.5 67 13612.5 68 10259.5 69 6829.0 70 5263.5 71 4254.0 72 3767.0 73 3380.5 74 2766.5 75 2337.0 76 1610.5 77 947.5 78 674.5 79 504.0 80 344.5 81 226.0 82 161.0 83 102.5 84 51.5 85 28.5 86 19.0 87 8.5 88 5.5 89 5.5 90 3.5 91 2.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021134277115231293 2 0.012347597351787212 3 0.009341627959030024 4 0.0 5 0.0035609175883431284 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.092994518961654E-4 14-15 2.0810557334472826E-4 16-17 2.3122841482747587E-5 18-19 0.0011330192326546318 20-21 0.0 22-23 6.936852444824276E-5 24-25 0.0 26-27 0.0015261075378613408 28-29 0.0030522150757226816 30-31 0.0012486334400683696 32-33 0.006867483920376033 34-35 0.00201168720899904 36-37 4.624568296549517E-4 38-39 0.0010867735496891365 40-41 2.5435125631022347E-4 42-43 0.004393339881722041 44-45 0.0018035816356543118 46-47 1.3873704889648552E-4 48-49 3.699654637239614E-4 50-51 2.3122841482747587E-5 52-53 1.3873704889648552E-4 54-55 0.0012717562815511172 56-57 0.013133773962200628 58-59 0.024371474922815953 60-61 0.018821992966956535 62-63 0.011908263363615007 64-65 0.01639409461126804 66-67 0.00210417857493003 68-69 5.087025126204469E-4 70-71 1.1561420741373793E-4 72-73 0.0 74-75 0.0015261075378613408 76-77 0.0020810557334472826 78-79 0.008879171129375073 80-81 0.014891109914889445 82-83 0.01581602357419935 84-85 0.027631795571883364 86-87 0.010012190362029705 88-89 0.03866139095915396 90-91 0.0445114698542891 92-93 0.02788614682819359 94-95 0.02906541174381372 96-97 0.04754056208852904 98-99 0.050292180224976 100-101 0.05653534742531785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2162364.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.59595420954464 #Duplication Level Percentage of deduplicated Percentage of total 1 72.03990078923923 29.965684144893334 2 14.48560832415285 12.050854010977211 3 5.3083364257063 6.624159566776114 4 2.453950060051768 4.082975773216906 5 1.4044998462906628 2.9210755646809448 6 0.8400823269247284 2.096641560180523 7 0.5947186681651706 1.731652334199187 8 0.4282814297443951 1.4251819792356932 9 0.29342460302631995 1.0984748716292956 >10 1.7995798915150347 14.174067514738798 >50 0.18996464208342517 5.532163833078711 >100 0.14749627974417628 11.872630787899766 >500 0.009474965631930714 2.5406381461437673 >1k 0.004347337172297622 2.9270614463219284 >5k 3.3441055171520173E-4 0.9567384660278587 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7350 0.3399057697963895 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6865 0.31747661355812434 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6419 0.29685103895551346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3935 0.18197676246922348 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3350 0.1549230379344088 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2652 0.12264355122449319 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2528 0.11690908653677179 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2480 0.11468929375442803 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2444 0.11302444916767021 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2311 0.10687377333325934 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2236 0.1034053471108472 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2207 0.10206422230484784 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.3122841482747584E-5 0.0 20-21 0.0 0.0 0.0 6.936852444824274E-5 0.0 22-23 0.0 0.0 0.0 2.081055733447283E-4 0.0 24-25 0.0 0.0 0.0 3.6996546372396134E-4 0.0 26-27 0.0 0.0 0.0 5.54948195585942E-4 0.0 28-29 0.0 0.0 0.0 0.0010174050252408939 0.0 30-31 0.0 0.0 0.0 0.002289161306792011 0.0 32-33 0.0 0.0 0.0 0.004925165235825236 0.0 34-35 0.0 0.0 0.0 0.00728369506706549 0.0 36-37 0.0 0.0 0.0 0.009064153861237052 0.0 38-39 0.0 0.0 0.0 0.010474647191684656 0.0 40-41 0.0 0.0 0.0 0.012694439974028424 0.0 42-43 0.0 0.0 0.0 0.015469180951958136 0.0 44-45 0.0 0.0 0.0 0.018637010235094554 0.0 46-47 0.0 0.0 0.0 0.02286849022643736 0.0 48-49 0.0 0.0 0.0 0.028186743767469307 0.0 50-51 0.0 0.0 0.0 0.03627973828643096 0.0 52-53 0.0 2.3122841482747584E-5 0.0 0.05431555464297408 0.0 54-55 0.0 4.624568296549517E-5 0.0 0.07563481449006734 0.0 56-57 0.0 4.624568296549517E-5 0.0 0.10093120307219322 0.0 58-59 0.0 4.624568296549517E-5 0.0 0.1308059142679031 0.0 60-61 0.0 4.624568296549517E-5 0.0 0.17427685625546854 0.0 62-63 0.0 4.624568296549517E-5 0.0 0.20896111847958992 0.0 64-65 0.0 4.624568296549517E-5 0.0 0.24121748234802282 0.0 66-67 0.0 4.624568296549517E-5 0.0 0.3034179259366138 0.0 68-69 0.0 4.624568296549517E-5 0.0 0.38178123572164535 0.0 70-71 0.0 4.624568296549517E-5 0.0 0.4476350882645105 0.0 72-73 0.0 4.624568296549517E-5 0.0 0.5061590000573447 0.0 74-75 0.0 4.624568296549517E-5 0.0 0.6125703165609491 0.0 76-77 0.0 4.624568296549517E-5 0.0 0.6958587915818059 0.0 78-79 0.0 4.624568296549517E-5 0.0 0.771424237547425 0.0 80-81 0.0 4.624568296549517E-5 0.0 0.8658116764800006 0.0 82-83 0.0 4.624568296549517E-5 0.0 1.0130116853591717 0.0 84-85 0.0 4.624568296549517E-5 0.0 1.1675416349883738 0.0 86-87 0.0 4.624568296549517E-5 0.0 1.3628833998346255 0.0 88-89 0.0 4.624568296549517E-5 0.0 1.5988057514830991 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10500 0.0 25.067768 1 GTAAGGT 565 0.0 24.377779 4 TACATGG 10980 0.0 23.535936 2 TAAGGTG 680 0.0 23.048868 5 GTACTAG 230 3.621608E-9 22.722708 1 ACATGGG 11025 0.0 22.40265 3 GAGTACT 7685 0.0 22.0931 12-13 GTACTTT 7945 0.0 21.309834 14-15 GATGTGC 1435 0.0 21.181295 8 GTATCAA 16675 0.0 20.827961 1 AGTACTT 8070 0.0 20.715414 12-13 GGTATCA 13185 0.0 20.683641 1 TACTTTT 8465 0.0 19.551956 14-15 CATGGGG 5735 0.0 19.213175 4 AGAGTAC 14155 0.0 19.074146 10-11 ACTTTTT 9010 0.0 18.843678 16-17 CATGGGA 5435 0.0 18.351192 4 ATGGGAG 2440 0.0 18.297115 5 ATGGGGA 3170 0.0 18.278692 5 CGTATAG 130 0.0075550266 18.273506 1 >>END_MODULE