FastQCFastQC Report
Thu 26 May 2016
SRR936463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576763
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT127430.8081747225169541No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75080.4761654097667183No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57080.3620074798812504No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC48490.3075287788970188No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39890.25298665684062854No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34430.21835875144203665No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33750.2140461185352523No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC32390.2054208527216836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30350.19248295400133056No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27270.17294926377648387No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26430.167621893715162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.1640703136742808No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA25360.16083583899419254No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA21080.1336916201103146No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA15970.10128345223727345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG45700.023.909781
TACATGG46600.022.5311872
GAGTACT38000.021.62850212-13
ACATGGG46500.021.1479153
GTACTTT42150.019.78109714-15
GTATAGC7800.019.4903351
AGAGTAC63700.018.30918910-11
CATGGGG27000.017.9467814
AGTGTCG1600.001190241117.814828
AGTACTT41200.017.58476612-13
GTCTAGG5850.017.0540431
GTATTAG5300.017.031061
GTGTACG2304.2666157E-516.5244161
GTGTAGC10100.016.4630621
ACTTTTT52250.016.4122716-17
GTATAGG4651.2732926E-1116.3467331
GTAAGCG2108.58563E-1015.83866294-95
TATAGCC8100.015.83642
GTATAAG6000.015.8358971
ATAGCCC6900.015.8353963
Produced by FastQC (version 0.11.5)