##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936463_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1576763 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.584560266825136 31.0 31.0 34.0 30.0 34.0 2 31.671694477863827 33.0 31.0 34.0 30.0 34.0 3 31.70074069470174 33.0 31.0 34.0 30.0 34.0 4 35.30534202032899 37.0 35.0 37.0 33.0 37.0 5 35.32120997258307 37.0 35.0 37.0 33.0 37.0 6 35.303471098700314 37.0 35.0 37.0 33.0 37.0 7 35.222789347543035 37.0 35.0 37.0 32.0 37.0 8 35.1895529004676 37.0 35.0 37.0 32.0 37.0 9 36.73586645551678 39.0 37.0 39.0 32.0 39.0 10-11 36.60947713765481 39.0 37.0 39.0 32.0 39.0 12-13 36.54029235845844 39.0 37.0 39.0 32.0 39.0 14-15 37.678520804965615 40.0 37.0 41.0 32.0 41.0 16-17 37.59543285833064 40.0 37.0 41.0 32.0 41.0 18-19 37.55763104537587 40.0 37.0 41.0 32.0 41.0 20-21 37.40958691953071 40.0 37.0 41.0 32.0 41.0 22-23 37.53115369906574 40.0 37.0 41.0 32.0 41.0 24-25 37.58267127019089 40.0 37.0 41.0 32.0 41.0 26-27 37.48813233187232 40.0 37.0 41.0 32.0 41.0 28-29 37.37505731679396 40.0 37.0 41.0 31.5 41.0 30-31 37.2053066947918 39.5 37.0 41.0 31.0 41.0 32-33 37.037274783845135 39.0 36.0 41.0 31.0 41.0 34-35 36.859341892218424 39.0 36.0 41.0 30.0 41.0 36-37 36.66263509481133 39.0 36.0 41.0 30.0 41.0 38-39 36.4764412914306 39.0 35.0 40.0 30.0 41.0 40-41 36.315754174850625 39.0 35.0 40.0 30.0 41.0 42-43 36.10540138245253 39.0 35.0 40.0 29.5 41.0 44-45 35.8679072251188 38.0 35.0 40.0 28.5 41.0 46-47 35.76364995880802 39.0 35.0 40.0 28.0 41.0 48-49 35.63575629311444 39.0 35.0 40.0 27.5 41.0 50-51 34.67769823365972 38.0 34.0 39.5 26.0 40.5 52-53 34.810369408719 38.0 34.0 39.5 26.5 40.5 54-55 35.26942571584949 38.0 35.0 40.0 27.0 41.0 56-57 35.153223090597635 38.0 34.0 40.0 26.5 41.0 58-59 34.93288750433642 38.0 34.0 40.0 26.0 41.0 60-61 34.61072875251385 38.0 34.0 40.0 26.0 41.0 62-63 34.28086085226505 37.5 34.0 40.0 25.0 41.0 64-65 33.942986041656226 37.0 33.0 40.0 24.5 41.0 66-67 33.44121786216445 36.0 33.0 39.0 23.0 41.0 68-69 33.053160811104775 36.0 32.0 39.0 22.0 40.5 70-71 32.630466024380326 35.0 32.0 38.5 21.5 40.0 72-73 32.22247002244472 35.0 31.5 38.0 20.5 40.0 74-75 31.807606152605054 35.0 31.0 37.0 20.0 39.0 76-77 31.339314786052185 35.0 31.0 36.5 18.0 39.0 78-79 30.831718527134388 34.5 30.5 36.0 16.0 39.0 80-81 30.341661999932775 34.0 30.0 36.0 11.5 37.5 82-83 29.918445574889823 34.0 29.5 35.0 7.5 37.0 84-85 29.55025517468383 34.0 29.0 35.0 7.0 36.5 86-87 29.22450235070204 34.0 29.0 35.0 2.0 36.0 88-89 28.799884319964384 34.0 29.0 35.0 2.0 36.0 90-91 28.371237465617853 34.0 29.0 35.0 2.0 35.0 92-93 27.910945716001706 34.0 27.0 35.0 2.0 35.0 94-95 27.398860513596528 33.0 26.5 35.0 2.0 35.0 96-97 26.805934056037593 33.0 25.0 35.0 2.0 35.0 98-99 26.068107889391115 33.0 24.5 35.0 2.0 35.0 100-101 23.111782176522407 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28230648880907694 1101 2 0.21421245193268135 1101 3 0.3408554913116397 1101 4 0.39798883992263967 1101 5 0.29613168236470244 1101 6 0.3206624466264998 1101 7 0.3429031496643091 1101 8 0.37459129118107626 1101 9 0.2975901860106873 1101 10-11 0.46429201738098413 1101 12-13 0.46333976105729846 1101 14-15 0.4236675157853753 1101 16-17 0.5723985907623046 1101 18-19 0.37003508322974454 1101 20-21 0.4817870639762134 1101 22-23 0.4211482488439344 1101 24-25 0.3342688074156115 1101 26-27 0.32090794320588856 1101 28-29 0.3610931398250372 1101 30-31 0.48219214684095846 1101 32-33 0.360391170292921 1101 34-35 0.22308148883770684 1101 36-37 0.17504004353258296 1101 38-39 0.04348490349339329 1101 40-41 0.031590380285898334 1101 42-43 0.30000516903626107 1101 44-45 0.40359432451167976 1101 46-47 0.3154828743422655 1101 48-49 0.35573863879280765 1101 50-51 0.36997873980207174 1101 52-53 0.38257951193772044 1101 54-55 0.45235607726154825 1101 56-57 0.28187704298512983 1101 58-59 0.25211050212308095 1101 60-61 0.30057852671075125 1101 62-63 0.21921913802020754 1101 64-65 0.07541516270862303 1101 66-67 0.3116702555099451 1101 68-69 0.24261660371453075 1101 70-71 0.24204531863056644 1101 72-73 0.15970443134275314 1101 74-75 0.06738351097832762 1101 76-77 0.2872982704166418 1101 78-79 0.22757234673534654 1101 80-81 0.09947560457732152 1101 82-83 -0.0031689645479957562 1101 84-85 0.2546499882826545 1101 86-87 0.19204778513130805 1101 88-89 0.24839537265431488 1101 90-91 0.3676960952767736 1101 92-93 0.3625217935884635 1101 94-95 0.3300340047435846 1101 96-97 0.38979868283852426 1101 98-99 0.34605787246410813 1101 100-101 0.43251750417381984 1102 1 0.25065884867368027 1102 2 0.313643470925939 1102 3 0.29994013063938496 1102 4 0.25831353115743383 1102 5 0.3910516892614382 1102 6 0.4040367671615428 1102 7 0.3914310133992629 1102 8 0.3879684702331403 1102 9 0.5024360529580605 1102 10-11 0.49217254044422987 1102 12-13 0.487268384341057 1102 14-15 0.41186478975834717 1102 16-17 0.27514707756320433 1102 18-19 0.30504703909060993 1102 20-21 0.28214653383473376 1102 22-23 0.3122706251336709 1102 24-25 0.37999158117533227 1102 26-27 0.12159512162272534 1102 28-29 -0.08336451328711547 1102 30-31 0.1874230061202553 1102 32-33 0.09016364595015602 1102 34-35 0.21130224348620175 1102 36-37 0.06943984570505535 1102 38-39 0.04714040228192573 1102 40-41 -0.210115723328542 1102 42-43 0.022794128808307335 1102 44-45 0.16679318654966835 1102 46-47 0.24190941045486625 1102 48-49 0.10340429666007367 1102 50-51 0.40497855830892604 1102 52-53 0.3364621040228073 1102 54-55 0.1688457503016494 1102 56-57 0.15806264694894168 1102 58-59 0.24603524599087478 1102 60-61 0.12620240939357785 1102 62-63 0.2826188902820732 1102 64-65 0.37681742411319874 1102 66-67 0.2631780098045269 1102 68-69 0.29756093069257616 1102 70-71 0.2678704318369185 1102 72-73 0.1384467065408046 1102 74-75 0.17232114539668686 1102 76-77 0.2890307359102593 1102 78-79 0.29662016564148885 1102 80-81 0.40281190229867647 1102 82-83 0.3784793561866877 1102 84-85 -0.009602554890026482 1102 86-87 0.15638857934530748 1102 88-89 0.3602729646032792 1102 90-91 0.2836626143290175 1102 92-93 0.29043657348265484 1102 94-95 0.19624644700607874 1102 96-97 0.3973617290194582 1102 98-99 0.34838166530783 1102 100-101 0.527676401421477 1103 1 0.3090440312984093 1103 2 0.16453066991199705 1103 3 0.2606018812250923 1103 4 0.3126823140433217 1103 5 0.27722905224488414 1103 6 0.39505915837404615 1103 7 0.4068312131150478 1103 8 0.4104643414932667 1103 9 0.18196134389019392 1103 10-11 0.4078963181451911 1103 12-13 0.12398680428190545 1103 14-15 0.42076558478081694 1103 16-17 0.553570090967213 1103 18-19 0.7391432291278619 1103 20-21 0.5537019165593193 1103 22-23 0.38540357055749297 1103 24-25 0.554045331607405 1103 26-27 0.5126155924910947 1103 28-29 0.5292454264140858 1103 30-31 0.3580811080521187 1103 32-33 0.45942325727250477 1103 34-35 0.6834971008046296 1103 36-37 0.8095251089842392 1103 38-39 0.7803825192692884 1103 40-41 0.4707715716747245 1103 42-43 0.4871535020079776 1103 44-45 0.60737677912946 1103 46-47 0.7626116803016245 1103 48-49 0.5814859688957412 1103 50-51 0.5375120683139158 1103 52-53 0.7566574030203839 1103 54-55 0.6595479241311963 1103 56-57 0.791115610039931 1103 58-59 0.7869011291571439 1103 60-61 0.6850647975335349 1103 62-63 0.7644097071964566 1103 64-65 0.8085357480368458 1103 66-67 0.8270284918108999 1103 68-69 0.8848540136130296 1103 70-71 0.6902414807288793 1103 72-73 0.5115854129970003 1103 74-75 0.6931573592462108 1103 76-77 0.762764749045413 1103 78-79 0.7067917483891151 1103 80-81 0.8473571648097185 1103 82-83 0.7121717938715193 1103 84-85 0.7502473702165524 1103 86-87 0.9042027476442662 1103 88-89 0.7342033329252793 1103 90-91 0.8588992967425035 1103 92-93 0.6527709438018157 1103 94-95 0.7256982847754081 1103 96-97 0.8351435395198976 1103 98-99 0.9956221307992479 1103 100-101 0.9341085940703984 1104 1 0.2765324750580369 1104 2 0.44853169713336527 1104 3 0.26659400586128257 1104 4 0.3546614272088746 1104 5 0.39986216301358724 1104 6 0.5028746009352503 1104 7 0.47106678921502976 1104 8 0.46233046030854297 1104 9 0.4517096746554756 1104 10-11 0.5424023958716049 1104 12-13 0.5161456811865506 1104 14-15 0.37152316053839485 1104 16-17 0.2840486049203008 1104 18-19 0.4710598529936405 1104 20-21 0.1569547842189678 1104 22-23 0.31963144249723285 1104 24-25 0.4427715771133123 1104 26-27 0.3865027693443821 1104 28-29 0.3104772360357231 1104 30-31 0.3741485622551579 1104 32-33 0.38417073201997454 1104 34-35 0.37622094946068074 1104 36-37 0.14659496003713457 1104 38-39 0.17386822377194733 1104 40-41 0.010751883265207596 1104 42-43 0.15798041520777417 1104 44-45 0.011280220321033596 1104 46-47 -0.13037753387403228 1104 48-49 0.2778350529566893 1104 50-51 0.010556067629110544 1104 52-53 0.18610013542922133 1104 54-55 -0.028763152739195164 1104 56-57 -0.08322525625008836 1104 58-59 0.07110760065175015 1104 60-61 0.06904442430977298 1104 62-63 0.23002347196276673 1104 64-65 0.2318237124265039 1104 66-67 0.3018316077157124 1104 68-69 0.3473518735685204 1104 70-71 0.1288093629408209 1104 72-73 -0.03261506637296918 1104 74-75 0.12455181225083223 1104 76-77 -0.009538794868301892 1104 78-79 0.09386587953104453 1104 80-81 0.2156374250391302 1104 82-83 0.13846010066830416 1104 84-85 -0.1095497535636234 1104 86-87 -0.12151202722305143 1104 88-89 0.20823175271641148 1104 90-91 0.029846052435022585 1104 92-93 -0.12764764890491165 1104 94-95 0.06794276346056449 1104 96-97 0.3051058747364692 1104 98-99 0.36679573121013576 1104 100-101 0.27545425406012924 1105 1 0.30091057951674927 1105 2 0.2229997252865914 1105 3 0.29059233955431196 1105 4 0.2580348623274702 1105 5 0.37466596205964464 1105 6 0.4069122166440451 1105 7 0.4860468219723515 1105 8 0.5067644763927532 1105 9 0.4339206766312884 1105 10-11 0.5139881814031781 1105 12-13 0.301981737325697 1105 14-15 0.2753826018571246 1105 16-17 0.33666735020453586 1105 18-19 0.42184566060879547 1105 20-21 0.34772685651260105 1105 22-23 0.1535565992154062 1105 24-25 0.33005954540663396 1105 26-27 0.13235122240588026 1105 28-29 0.01884089247534604 1105 30-31 0.03407722557918191 1105 32-33 -0.16214749915167914 1105 34-35 0.1451609707746826 1105 36-37 0.15083643067014663 1105 38-39 0.08888447695691326 1105 40-41 -0.012289495018265484 1105 42-43 0.12542218229619806 1105 44-45 0.07442840285860797 1105 46-47 0.14631516465657057 1105 48-49 0.18307597317303959 1105 50-51 0.20280999875843264 1105 52-53 0.1800731566478646 1105 54-55 0.028809760738319312 1105 56-57 0.09731851860440344 1105 58-59 0.24426466681384085 1105 60-61 0.3123712496771205 1105 62-63 0.17060356762484474 1105 64-65 0.24277307242075352 1105 66-67 0.4069473876092715 1105 68-69 0.4757252668621419 1105 70-71 0.3747502491952943 1105 72-73 0.1565521816608353 1105 74-75 0.31128735628500337 1105 76-77 0.33997578448120436 1105 78-79 0.6058458068894268 1105 80-81 0.7041292833896513 1105 82-83 0.3498301362529901 1105 84-85 0.5206229501198187 1105 86-87 0.3357215184138518 1105 88-89 0.3654309182245292 1105 90-91 0.451996391468267 1105 92-93 0.3795822161342599 1105 94-95 0.5533855811923445 1105 96-97 0.5657607869123886 1105 98-99 0.7627255756714213 1105 100-101 0.8999609284895094 1106 1 0.3888136534037976 1106 2 0.3293350869061946 1106 3 0.3167104117861754 1106 4 0.2840285671414051 1106 5 0.24475779276632892 1106 6 0.491181811066042 1106 7 0.6106538300763518 1106 8 0.5426795310077281 1106 9 0.43972936662171946 1106 10-11 0.5321418617338054 1106 12-13 0.5739150593196527 1106 14-15 0.5649453101649229 1106 16-17 0.5533079240992009 1106 18-19 0.5364360620046398 1106 20-21 0.28004110859193787 1106 22-23 0.536729718051518 1106 24-25 0.4283779333122695 1106 26-27 0.48156746381523163 1106 28-29 0.3696161465293315 1106 30-31 0.47414432302043963 1106 32-33 0.3355259461489126 1106 34-35 0.30387437154686125 1106 36-37 0.22899876185204704 1106 38-39 0.25478771666718103 1106 40-41 0.3729458977541569 1106 42-43 0.43333457154671606 1106 44-45 0.128068253105603 1106 46-47 0.1034765332102694 1106 48-49 0.09996455033052598 1106 50-51 -0.023547998950526505 1106 52-53 0.059711101147080115 1106 54-55 0.27522703415338157 1106 56-57 0.3045062542699881 1106 58-59 0.10429431990799998 1106 60-61 0.41438027119207277 1106 62-63 0.3291494686515435 1106 64-65 0.2872367104969342 1106 66-67 0.2974892759578225 1106 68-69 0.4175932532865829 1106 70-71 0.28605829570627606 1106 72-73 -0.015163405905106231 1106 74-75 0.23640978808257884 1106 76-77 0.17715018113157655 1106 78-79 0.039640732332991035 1106 80-81 0.32007919136385965 1106 82-83 0.16950225740636427 1106 84-85 0.29074258060227365 1106 86-87 0.07191978324451398 1106 88-89 0.187936056001071 1106 90-91 0.2130216545144954 1106 92-93 0.3578136504868574 1106 94-95 0.1932539651048124 1106 96-97 0.15840110109862593 1106 98-99 0.31347769372780476 1106 100-101 0.3974063668572221 1107 1 0.22748265482177743 1107 2 0.1806323648272503 1107 3 0.23808861294910955 1107 4 0.21619198704452458 1107 5 0.3492201496597147 1107 6 0.32773335799740266 1107 7 0.38575605475175223 1107 8 0.32447735296391045 1107 9 0.5377908251434036 1107 10-11 0.5274306444591801 1107 12-13 0.3379227741500799 1107 14-15 0.22113795252607105 1107 16-17 0.3442180964457293 1107 18-19 0.3137152202252764 1107 20-21 0.3791941267972945 1107 22-23 0.31421284979050057 1107 24-25 0.2391506217974353 1107 26-27 0.4412536681630783 1107 28-29 0.5227782118960178 1107 30-31 0.3788737805397062 1107 32-33 0.16460216499378788 1107 34-35 0.3093745190549271 1107 36-37 0.33542392767966334 1107 38-39 0.29269205530764 1107 40-41 0.43445501629037153 1107 42-43 0.34657825804290354 1107 44-45 0.30444919823577976 1107 46-47 0.5294837912415247 1107 48-49 0.6377991002088734 1107 50-51 0.4580907363502149 1107 52-53 0.5666086100054883 1107 54-55 0.6384428051447273 1107 56-57 0.3110223040991613 1107 58-59 0.44889393860757565 1107 60-61 0.4225112964207156 1107 62-63 0.5572448381671862 1107 64-65 0.4370368608599975 1107 66-67 0.5084058244594232 1107 68-69 0.7022568555921467 1107 70-71 0.2695900511791436 1107 72-73 0.25914148216323696 1107 74-75 0.48176804234841697 1107 76-77 0.4149090736473653 1107 78-79 0.2677230957611414 1107 80-81 0.40428662894256817 1107 82-83 0.4704580462255663 1107 84-85 0.2943793681621081 1107 86-87 0.2866030215699631 1107 88-89 0.19469811121667036 1107 90-91 0.4229123082131423 1107 92-93 0.47961994226098525 1107 94-95 0.6836078892951818 1107 96-97 0.43856761074057715 1107 98-99 0.5685933686009967 1107 100-101 0.4581198935054296 1108 1 0.2892555423824028 1108 2 0.21241555907205267 1108 3 0.1909794827436322 1108 4 0.46800737675093984 1108 5 0.17040917562635371 1108 6 0.20152990611996557 1108 7 0.25085534707206847 1108 8 0.18481523295825752 1108 9 0.35873579985928217 1108 10-11 0.5120011245989957 1108 12-13 0.3233021049394935 1108 14-15 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2316 74-75 -1.5371248203478949 2316 76-77 -1.5752738942684594 2316 78-79 -2.286545291736914 2316 80-81 -2.537697510382575 2316 82-83 -2.739866942112169 2316 84-85 -2.2908354766002397 2316 86-87 -2.1622229774970556 2316 88-89 -2.586712244871027 2316 90-91 -2.6064143905895314 2316 92-93 -3.202142873267963 2316 94-95 -3.082099880362204 2316 96-97 -3.300946720882287 2316 98-99 -4.2523831513803465 2316 100-101 -4.893720152106969 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2859.0 3 1071.0 4 1077.0 5 1168.0 6 1341.0 7 1639.0 8 1944.0 9 2604.0 10 3260.0 11 4145.0 12 5242.0 13 6245.0 14 7281.0 15 8581.0 16 10021.0 17 12108.0 18 10525.0 19 8864.0 20 9205.0 21 10059.0 22 11066.0 23 12617.0 24 14719.0 25 16879.0 26 20030.0 27 23700.0 28 28405.0 29 34574.0 30 42219.0 31 52254.0 32 67017.0 33 85913.0 34 114498.0 35 156898.0 36 222573.0 37 295546.0 38 235485.0 39 33124.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8808001796332 18.693904269206225 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23.038554404845037 44-45 22.626315093501375 26.72568712119266 27.72946611730585 22.918531668000114 46-47 23.294820508673713 27.35999264308331 27.450622246111063 21.89456460213192 48-49 22.81668264888835 27.196921265994906 27.673565659025645 22.312830426091104 50-51 21.891782443560253 27.568943685353652 28.38992873422302 22.149345136863083 52-53 22.74190039658454 27.91827047788659 27.011637501561793 22.328191623967076 54-55 22.21457955163845 27.124218449723912 27.589628547182233 23.071573451455404 56-57 22.86383383542096 27.093515141905815 27.217155541461867 22.82549548121135 58-59 23.578840213868467 26.966676603220712 27.080302155809395 22.37418102710142 60-61 22.60498624199564 27.30560337815533 28.23087951336906 21.85853086647997 62-63 22.226834369850973 27.46281284207936 27.97950726619371 22.330845521875954 64-65 22.019833213783553 28.22177150580086 27.36282058843862 22.39557469197697 66-67 22.480286765634048 27.36814128193271 26.926777965326526 23.22479398710671 68-69 22.284959958427702 27.362465455558343 27.407419627365766 22.94515495864819 70-71 23.07258602877906 27.150909847779133 27.03998685169062 22.736517271751186 72-73 22.669233906596194 26.74726979678801 28.037095369516997 22.5464009270988 74-75 22.05954424295792 28.28828937422304 27.155415378144355 22.496751004674685 76-77 22.355073463617423 27.294221462649347 27.064827070822027 23.285878002911204 78-79 22.60819073425963 27.40675400826879 27.28560957732714 22.699445680144432 80-81 23.111818099010115 27.25433060944804 26.971225119962778 22.662626171579063 82-83 22.766646128620625 27.169804376697797 27.716241194920865 22.347308299760712 84-85 22.456958521662433 27.7295495293768 27.37362854497713 22.439863403983637 86-87 22.434017362775176 27.83052216146918 27.07704204181821 22.658418433937435 88-89 22.18433725500893 27.80330872149654 26.5972769131371 23.41507711035743 90-91 22.518822550451222 27.126615893709893 27.008381122662467 23.346180433176418 92-93 23.140931278092797 27.51252013736706 27.15184756278783 22.194701021752323 94-95 22.59502551182221 27.39057572228523 27.253026278064908 22.761372487827654 96-97 21.70010777359046 27.421623099451743 28.130619815497194 22.747649311460602 98-99 22.265255545920915 27.94270359896707 26.741651889494992 23.050388965617017 100-101 22.145478167294847 27.22965809174823 27.025987515101548 23.598876225855374 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 88.0 1 61.5 2 60.0 3 74.0 4 71.5 5 90.5 6 138.5 7 270.5 8 448.5 9 565.0 10 570.0 11 553.5 12 602.5 13 693.5 14 799.0 15 928.5 16 1035.0 17 1146.5 18 1312.0 19 1475.5 20 1727.0 21 2078.0 22 2506.0 23 3212.0 24 4155.0 25 5345.5 26 7126.5 27 9416.5 28 11664.0 29 14336.5 30 17552.0 31 21220.0 32 25096.5 33 28206.5 34 31176.0 35 34020.0 36 37218.5 37 40624.0 38 43188.5 39 45115.0 40 47433.0 41 49832.0 42 51666.5 43 53478.5 44 55092.5 45 57346.0 46 59931.5 47 70122.0 48 89270.5 49 86648.0 50 65970.5 51 55731.0 52 52873.0 53 51021.0 54 48205.0 55 44198.0 56 39837.0 57 35296.5 58 31823.5 59 28274.0 60 23300.5 61 19293.0 62 15739.5 63 11779.0 64 8342.5 65 6240.0 66 4707.0 67 3685.5 68 3051.5 69 2479.5 70 1970.0 71 1445.5 72 1099.5 73 926.0 74 748.5 75 587.5 76 445.0 77 296.5 78 199.0 79 141.5 80 101.0 81 68.0 82 35.5 83 25.0 84 23.0 85 15.0 86 10.0 87 8.5 88 6.5 89 4.5 90 4.0 91 3.5 92 2.0 93 0.5 94 1.0 95 1.5 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01376237265841474 2 0.01281105657603584 3 0.0150942151737452 4 0.020231322018591253 5 0.03367658931621302 6 0.052512647747315226 7 0.00678605472096948 8 0.026256323873657613 9 0.03672080077982551 10-11 0.042714092098812564 12-13 0.03434251057387826 14-15 0.029839614450618137 16-17 0.031456851790662266 18-19 0.024290270636741225 20-21 0.05707896494273394 22-23 0.013667241050176849 24-25 0.0029807903914538837 26-27 0.0014903951957269419 28-29 6.342107215859327E-5 30-31 0.0027271061028195107 32-33 0.002188026989471468 34-35 0.0 36-37 2.536842886343731E-4 38-39 3.1710536079296635E-5 40-41 0.0014269741235683486 42-43 0.003551580040881223 44-45 0.0013635530514097553 46-47 9.830266184581958E-4 48-49 6.976317937445259E-4 50-51 0.0037735537934363 52-53 0.0031710536079296637 54-55 0.005644475422114801 56-57 8.244739380617126E-4 58-59 0.005898159710749174 60-61 0.0028222377110574005 62-63 5.707896494273394E-4 64-65 8.878950102203057E-4 66-67 0.018011584493040488 68-69 0.0452509349851563 70-71 0.05676185958194097 72-73 0.014713688740793637 74-75 0.032122773048327495 76-77 0.02781014014154315 78-79 0.060757387127932355 80-81 0.08742594797062082 82-83 0.05238580560299805 84-85 0.018360400389912754 86-87 0.021848559358635382 88-89 0.02241934900806272 90-91 0.041953039232909446 92-93 0.02552698154383379 94-95 0.01515763624590379 96-97 0.019660532369163916 98-99 0.054066464015200766 100-101 0.07480515461106076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1576763.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.6708778879661 #Duplication Level Percentage of deduplicated Percentage of total 1 81.68268523002182 53.64173647301935 2 11.47474965578351 15.071137668798801 3 3.295035706547294 6.491636625634664 4 1.3184339440472175 3.4633085817149745 5 0.6757374820530038 2.2188136834112244 6 0.39302272190755994 1.548608830655246 7 0.23300824374013146 1.071129914508333 8 0.17287161921541008 0.9082104796632136 9 0.1279839825858849 0.7564338442811904 >10 0.5770373202226546 6.574940287579271 >50 0.03237857607904314 1.428423021125386 >100 0.01368313839822141 1.7340283244465187 >500 0.0010598906680108415 0.43002586227772954 >1k 0.0020234276389297884 3.018976444251435 >5k 1.9270739418378934E-4 0.8362598287025416 >10k+ 9.635369709189467E-5 0.8063301299301218 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12743 0.8081747225169541 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7508 0.4761654097667183 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5708 0.3620074798812504 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4849 0.3075287788970188 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3989 0.25298665684062854 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3443 0.21835875144203665 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3375 0.2140461185352523 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3239 0.2054208527216836 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3035 0.19248295400133056 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2727 0.17294926377648387 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2643 0.167621893715162 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2587 0.1640703136742808 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2536 0.16083583899419254 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2108 0.1336916201103146 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1597 0.10128345223727345 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 6.342107215859327E-5 0.0 9 0.0 0.0 0.0 6.342107215859327E-5 0.0 10-11 0.0 0.0 0.0 6.342107215859327E-5 0.0 12-13 6.342107215859327E-5 0.0 0.0 6.342107215859327E-5 0.0 14-15 6.342107215859327E-5 0.0 0.0 6.342107215859327E-5 3.1710536079296635E-5 16-17 6.342107215859327E-5 0.0 0.0 6.342107215859327E-5 6.342107215859327E-5 18-19 6.342107215859327E-5 0.0 0.0 6.342107215859327E-5 1.902632164757798E-4 20-21 6.342107215859327E-5 0.0 0.0 9.513160823788991E-5 1.902632164757798E-4 22-23 6.342107215859327E-5 0.0 0.0 1.9026321647577982E-4 1.902632164757798E-4 24-25 1.5855268039648317E-4 0.0 0.0 4.7565804118944953E-4 1.902632164757798E-4 26-27 2.2197375255507645E-4 0.0 0.0 6.02500185506636E-4 1.902632164757798E-4 28-29 2.536842886343731E-4 0.0 0.0 0.0010147371545374923 1.902632164757798E-4 30-31 3.1710536079296634E-4 0.0 0.0 0.0019026321647577981 1.902632164757798E-4 32-33 3.1710536079296634E-4 0.0 0.0 0.0037735537934363 1.902632164757798E-4 34-35 3.1710536079296634E-4 0.0 0.0 0.005263948989163242 1.902632164757798E-4 36-37 3.1710536079296634E-4 0.0 0.0 0.00669092311273159 1.902632164757798E-4 38-39 3.1710536079296634E-4 0.0 0.0 0.009164344926916729 1.902632164757798E-4 40-41 3.1710536079296634E-4 0.0 0.0 0.012303687998767094 1.902632164757798E-4 42-43 3.1710536079296634E-4 0.0 0.0 0.01807500556519908 1.902632164757798E-4 44-45 3.1710536079296634E-4 0.0 0.0 0.029141982656873608 1.902632164757798E-4 46-47 3.1710536079296634E-4 0.0 0.0 0.04125540743916492 1.902632164757798E-4 48-49 3.1710536079296634E-4 0.0 0.0 0.06354791430291046 1.902632164757798E-4 50-51 3.1710536079296634E-4 0.0 0.0 0.0875845006510173 1.902632164757798E-4 52-53 3.1710536079296634E-4 0.0 0.0 0.1130797716587718 1.902632164757798E-4 54-55 3.1710536079296634E-4 0.0 0.0 0.16121636542714407 1.902632164757798E-4 56-57 3.1710536079296634E-4 0.0 0.0 0.212841118164239 1.902632164757798E-4 58-59 3.805264329515596E-4 0.0 0.0 0.2662416609217746 2.2197375255507645E-4 60-61 3.805264329515596E-4 0.0 0.0 0.32538181070966277 2.536842886343731E-4 62-63 3.805264329515596E-4 0.0 0.0 0.4019944658772434 2.536842886343731E-4 64-65 3.805264329515596E-4 0.0 0.0 0.45951737832508754 2.536842886343731E-4 66-67 3.805264329515596E-4 0.0 0.0 0.551287669738572 2.536842886343731E-4 68-69 3.805264329515596E-4 0.0 0.0 0.6785737615608687 2.536842886343731E-4 70-71 3.805264329515596E-4 0.0 0.0 0.7666022097169961 3.1710536079296634E-4 72-73 3.805264329515596E-4 0.0 0.0 0.8967739603225089 3.1710536079296634E-4 74-75 3.805264329515596E-4 0.0 0.0 1.0534874296263927 3.1710536079296634E-4 76-77 3.805264329515596E-4 0.0 0.0 1.1727507558206276 3.1710536079296634E-4 78-79 3.805264329515596E-4 0.0 0.0 1.2947094775816024 3.1710536079296634E-4 80-81 3.805264329515596E-4 0.0 0.0 1.4765059809242098 3.1710536079296634E-4 82-83 3.805264329515596E-4 0.0 0.0 1.6758384107186686 3.805264329515596E-4 84-85 3.805264329515596E-4 0.0 0.0 1.9011100590259917 3.805264329515596E-4 86-87 3.805264329515596E-4 0.0 0.0 2.204738441985257 3.805264329515596E-4 88-89 3.805264329515596E-4 0.0 0.0 2.550510127393908 3.805264329515596E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4570 0.0 23.90978 1 TACATGG 4660 0.0 22.531187 2 GAGTACT 3800 0.0 21.628502 12-13 ACATGGG 4650 0.0 21.147915 3 GTACTTT 4215 0.0 19.781097 14-15 GTATAGC 780 0.0 19.490335 1 AGAGTAC 6370 0.0 18.309189 10-11 CATGGGG 2700 0.0 17.946781 4 AGTGTCG 160 0.0011902411 17.81482 8 AGTACTT 4120 0.0 17.584766 12-13 GTCTAGG 585 0.0 17.054043 1 GTATTAG 530 0.0 17.03106 1 GTGTACG 230 4.2666157E-5 16.524416 1 GTGTAGC 1010 0.0 16.463062 1 ACTTTTT 5225 0.0 16.41227 16-17 GTATAGG 465 1.2732926E-11 16.346733 1 GTAAGCG 210 8.58563E-10 15.838662 94-95 TATAGCC 810 0.0 15.8364 2 GTATAAG 600 0.0 15.835897 1 ATAGCCC 690 0.0 15.835396 3 >>END_MODULE