FastQCFastQC Report
Thu 26 May 2016
SRR936463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576763
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT136020.8626534235011857No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT76110.48269778019905335No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT62400.39574749026962197No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53010.33619510351270293No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT44190.28025771786882364No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT39900.2530500779127871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38830.24626402319181767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35070.2224177000601866No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33120.2100505909892609No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA31320.19863479800071412No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30580.19394163866097822No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27790.17624715952873068No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23840.15119583602608636No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23310.14783451920168092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.12176845854449908No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT16640.10553266407189921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC1102.7325932E-630.2296943
GTATAGA5450.022.6645221
GAGTACT55800.020.98410612-13
CACGACC1856.5519E-620.5415344
GTACTTT57950.020.00096914-15
TACATGG42000.019.906992
GTACATG42500.019.8976061
GGACCGT1200.00476643219.7901156
AGTACTT58700.019.25957112-13
GTGTAGC9700.019.1012861
GCGCTAC2251.6656395E-619.003331
TAAGGTG7750.019.000925
GTAAGGT6250.019.000924
AGGCGAC3054.5638444E-918.6894285
AGAGTAC80400.018.46268510-11
TAGATCG650.00756248218.27069574-75
ACATGGG42800.018.2023933
TATTACG1350.009395855517.5945592
TACTTTT65150.017.17082214-15
TAGACCG1700.001787382816.7655165
Produced by FastQC (version 0.11.5)